한국에서 분리된 유행성이하선염 바이러스 변이에 관한 연구

민경일(Kyung Il Min),김도근(Do Keun Kim),조수열(Soo Yeul Cho),안광수(Kwang Soo Ahn),민복순(Bok Soon Min),김병국(Myoung Guk Kim),반상자(Ssang Ja Ban),허숙진(Sook Jin Hur),박순희(Sue Nie Park),이길웅(Kil Ung Lee) 대한바이러스학회 2000 Journal of Bacteriology and Virology Vol.30 No.2

국내 사람 로타바이러스의 혈청형 항원부위의 염기서열 비교분석에 관한 연구

민복순,김재근,백선영,신진호,김재옥,민경일,류승렬,김병국,김도근,박미경,안미진,신계명,이석호,박순희 식품의약품안전청 2000 식품의약품안전청 연보 Vol.4 No.-

사람 로타바이러스는 영 ·유아 설사질환의 주요 원인체로 매년 1억 2천 5백만 명 이상의 설사 환자를 유발시키고 있다. 발병률이 높음에도 불구하고, 국내는 유행 사람 로타바이러스의 발병률과 특성에 관한 연구 자료가 미흡한 실정이다. 따라서 현재 외국에서 개발되어 시판되고 있는 사람 로타바이러스 진단제제와 향후 국내 ·외에서 개발될 백신 품질효능 평가시 많은 어려움이 있다. 이에 본 연구에서는2000년 1월에서 4월 사이에 서울, 대전 등 총 9개 지역에서 로타바이러스 감염이 의심되는 국내 영 · 유아 설사 환자를 대상으로 하여 총 가김물 30f건을 수거하여 실험에 사용하였다 가검물에서 사람로타바이러스 양성를을 조사하기 위하여 라텍스응짇법(L.AT), 효소면역법(ELISA),폴리아크릴아마이드 겔 전기영동법(PAGE), 핵산 증폭법(RT-PCR)시험을 시행하였다. 그 결과 LAT는 71.9%, ELISA 64.9%, PAG료 53.0%, VPf-RT-PCR 68.5%, VP4-RT-PCR 69.5%이었다. VP7-PCR과 LAT간의 일치도는 96.7%,'#P4-PCR과 LAT간의 일31도는 98.0%를 나타내었다. PAGE시험으로 group을 조사한 결과 총 302건 중 159건이 전세계적으로 사람에게 감염하는 A 잉cuP을 나타내었으며,1건은 극히 일부에서 발생하는 C: group을 나타내었다. RT-PfR에 의한 VP7 혈청형은 C2 type 50건, Gl type 44건, G4 type 26건이며, VP4의 혈청형은 PIB[41 type 줬건, P2A(6) type 47건, PIA(8) type 템건이었다. 현재 세계적으로 Gl type과 PIA[81type의 혈청형이 유행하는 것으로 알려져 있으나, 이와 다르fl 본 실험의 경우 G2 type과 PIB14T type이 가장 높은 경향을 나타내었다. Human rotaviruses are the leading cause of severe diarrheal disease of infants and children younger than 2 :fears of age worldwide. Thousands of children in less devetopedcountries died from rotavirus infecdon every year. Current research efforts are directed atimproving the effectiveness of rotavirus vaccines and reducing adverse effects of vaccination.It is expected that safer and more effective vaccines as weIB as a variety of diagnostic kitsincludhg serotyping Icits will be commercially available in the near future. In this study weinvestigated human rotavinls infection in Korean infants and young children who visil~edhospital due to diarrheal illness. A total of 302 fecal samples were collected from 9 urban areasacross the national terftory from January to April 2000. Delfction (If rotaviruses in fecalsamples was peformed using latex agglutination test (LAT), enzyme-linked immunosorbentassay (ELISA), polyacrylamide gel electrophoresis (PAGE), and reverse transcription-polymerasechain reaction (RT-PCR). G and P typug analysis was performed by multiplex PCR andsequencing. Of 30f samples tested and the positive rates were 71.9% (fA7), 64.9% (196), 53.0%(t60), 69.5% (211), and 68.5% (207) by LAT, ELISA, PAGE, VPT-PCR, and VP4-PCR,respectively The G(VP7) and P(VP4) typing and phylogenetic analysis revealed that Gl, C2,G4, PIA141, PIB[87, and P2A[6] types of gioup A rotavirus were prevailing in the givensampling frame during the study period. The most prevalent type was G2 and PIB[47 type. Itwas an unexpected finding in that the world-most prevalent type has been reported to be Gl,PIA[8B. A further study with greater sample size and expanded time period of sample collectionis necessary to confirm the difference.

수두백신의 역가시험법 표준화를 위한 연구

민경일,백선영,신진호,김재옥,류승렬,민복순,김병국,김도근,김훈,이석호,박순희 식품의약품안전청 2001 식품의약품안전청 연보 Vol.5 No.-

수두(chicltenpo즈) 및 대상포진(shingles)의 예방을 위해 사용되는 수두 생 바이러스 백근은 현재 국내 -외 제조사들이 정상사람 2배체 폐 태아 세포 주들을 이용하여 바이러스를 배양 · 증식시켜 생산하고 있으나, 품질관리를 쒸한 역가 시험에는 t·fRC튼 띤 LB딘EL 세포 주외에 제조사가 설정한 세포 주를 이용하고 있으며, 역파신험 방법인 fH Ui'rFO Plaque 3SSaIT에도 조사별로 다소 차이가 있는 시헌법을 사용하고 있다. 그러므로 일반적으로 세포 주간우 감수성 차이, 계대력(passa딩e level)간의 차이,varicelta-zoster virus(4'2V) diluent medium 등에 의한 차이에 의해 역가시힘 결과에 많은 차이를 나타낼 수 있다. 이에 본 연구에서는 역가 시험 결과꼭 편차를 촉소화 할 수 있는 시헌방법을 극힙하고자 Ok퍼 백신 주와 세로 주간 및 기타 요인에 따른 차이를 비교 분석하고 이를 표준확하여 일관겅있는 국가 및 제조사의 품질관리예 활용하고 나아가 수두 생 바이러스 백신 역가시헌 국가 표준품 거달에 활용하고자 하는데 목적이 운.다. 수두 생 바이러스 빅신의 역가 시험에 영향을 띠칠 수 꿀.는 모민으로 우선 바이러스 흡착에 사용되는 1#Z)·'diluent medium이 가장 큰 오인으로 확인되.고. 현개까기 최종적으로 if4 sucrose, 10% FBS, 0.1% sodiun) rrlonog)utamate in PBS(-), fIH 7.2가 가당 두수글 L'z).「 린luent medium 조성으로 확인되었단. 또한 '·:~l포 주별로 다소의 차이는 잇.으나 최대 바이러스 흡착시간은 90분으론:÷잰착인 되었다. 다른 negati)Fe effect를 보일 수 있는 요인으로 항생물질 및 항츤 제인 fuugiBone이 곡이러스의 역가에 영향을 미치는 것으로 확인되었다 따라서 이러한 항생물 띤 항균제를 standard solid agarose overla?·(5.tO) 시헌방법에는 사용하지 않는 것이 역가 시험의 편차를 줄일 수 있는 요인이라 판단된다. 최적의 조건하데서 Oka 수두 백신 주의 j 종의 세포 쭈에 대한 역가시험 수행 결과 각 세포 주의 감수성의 차이는 크지 않았으나, 반복시헌에 대한 표준편차는 다소 차이를 나타났다. Until now in Korea, the international and domestic vaccine maufacturers produce and sale live attenuated daricella vaccines, used to prevent chickenpox and shinglees, which are propagated in and done the potency test with various originated human diploid embryonic lung cells. Also manufacturers perform in vitro plaque assay with a little different methods to their quality control tests. Therefore, in general, there is slight or big difference among in vitro plaque assay results because of the difference in susceptibility of various originated human diploid embryonic lung cells and in assay methods. The purpose of this study is to confirm what factors occurring deviation to establish the standardized method which minimize deviation intracellularly and intercellularly. Eventually the standadized method will be used in consistent national and manufacturer's quality control and moreover intra and inter laboratory's validation assay of national standard for live varicella vaccine candidate. We confirmed VZV diluent medium was the biggest factor influencing in vitor plaque assay and the excellent composition of VZV diluent medium was final 5% sucrose, 10% FBS, 0.1% sodium monoglutamate in PBS(-), pH 7.2. We confirmed 90 minutes was maximum and optimum VZV adsorption time, but a little different among cells Also we recommended not use antibiotics and antimycotic, fungizone in standard solid agarose overlay assay in varicella potency test. Eventually 5 different originated human diploid embryonic lung cells showed little difference in susceptibility to Oka vaccine strain, but showed a lot of difference in reproducibility.

Efficient Solar Cells Based on Light-Harvesting Antimony Sulfoiodide

Nie, Riming,Yun, Hyun-sung,Paik, Min-Jae,Mehta, Aarti,Park, Byung-wook,Choi, Yong Chan,Seok, Sang Il Wiley-VCH 2018 ADVANCED ENERGY MATERIALS Vol.8 No.7

<P> Although antimony sulfoiodide (SbSI) exhibits very interesting properties including high photoconductivity, ferroelectricity, and piezoelectricity, it is not applied to solar cells. Meanwhile, SbSI is predominantly prepared as a powder using a high-temperature, high-pressure system. Herein, the fabrication of solar cells utilizing SbSI as light harvesters is reported for the first time to the best of knowledge. SbSI is prepared by solution processing, followed by annealing under mild temperature conditions by a reaction between antimony trisulfide, which is deposited by chemical bath deposition on a mesoporous TiO<SUB>2</SUB> electrode and antimony triiodide, under air at a low temperature (90 °C) without any external pressure. The solar cells fabricated using SbSI exhibit a power conversion efficiency of 3.05% under standard illumination conditions of 100 mW cm<SUP>-2</SUP>. </P>

A SYMMETRIC FINITE VOLUME ELEMENT SCHEME ON TETRAHEDRON GRIDS

Nie, Cunyun,Tan, Min Korean Mathematical Society 2012 대한수학회지 Vol.49 No.4

We construct a symmetric finite volume element (SFVE) scheme for a self-adjoint elliptic problem on tetrahedron grids and prove that our new scheme has optimal convergent order for the solution and has superconvergent order for the flux when grids are quasi-uniform and regular. The symmetry of our scheme is helpful to solve efficiently the corresponding discrete system. Numerical experiments are carried out to confirm the theoretical results.

Dynamic Characteristic Simulation of Helicopter Tail Drive Shaft System

Nie Junfeng,Yu Guangbin,Song Ye,Qu Zhigang,Zheng Minli,Zhao Xingfu 보안공학연구지원센터 2016 International Journal of Smart Home Vol.10 No.6

Helicopter with the advantage of light, flexible and hovering in the air, is widely used. In both military and civilian domain, it gets more and more attention. It becomes the main subject of research in the world. In the power transmission system of helicopter, the tail rotor shaft is an important part. It is one of the key technologies to predict the dynamic characteristics of the shaft accurately for improving the overall performance of the helicopter. Based on the theory of elastic mechanics, rotor dynamics and the finite element analysis, the former main drive shaft is taken as an example. The dynamic characteristics of the transmission shaft is calculated by the multi/few degrees of freedom finite element method. The validity of the finite element method with few degrees of freedom is determined. Then the structure characteristics of the helicopter tail drive shaft are analyzed, and the dynamic characteristics of the helicopter tail rotor shaft are calculated by using the finite element method with few degrees of freedom. The research results provide the basis and guidance for the design of the helicopter tail drive system.

Study on Occurrence of Titanium Element in Anatase Ores of Shazi, Qinglong, Guizhou Province

Min Zhang,Aiguo Nie,Fei Xie,Zhuru Zhang 보안공학연구지원센터 2016 International Journal of u- and e- Service, Scienc Vol.9 No.2

The tectonic position of anatase deposit in Shazi area, Qinglong, Guizhou Province is located at the transitional area between Yangtze Platform and the western part of South China orogenic belt. Three industrial ore bodies in this deposit are yielded above the limestone Karst unconformity of the Middle Permian Maokou Formation and at the bottom of Emeishan basalt. Genetic type of deposit is hot water -eluvium talus sedimentary which is related to the eruption of Emeishan basalt. Based on the analysis of chemistry, X-ray phase, electron probe and microscopic identification, etc., the data suggest that TiO2 in anatase ores are mainly present in the form of microgranular inclusions in silicate and quartz; secondly, Ti occurs as isomorphism in limonite; few are as independent minerals existing in the anatase with particle size greater than 0.04mm. According to the occurrence of Ti, it is determined that the anatase ores are extremely refractory, using conventional processing methods (heavy, magnetic, flotation) are not effective in enrichment anatase, but "roasting- acid leaching-alkaline leaching" beneficiation process works and obtain anatase with 42.32% TiO2. Extraction method can also enrich the associated element Sc, obtaining 99.99% scandium oxide products, and the leaching rate of Sc is 90%, which can produce iron oxide red and poly aluminum silicate salt (PSA) coagulant by-product. Mineral resources can be effectively developed and utilized without emission, which can obtain good environmental benefits.

Research on Nonlinear Vibration Characteristics of Spiral Bevel Gear

Nie Junfeng,Yu Guangbin,Song Ye,Qu Zhigang,Zheng Minli,Zhao Xingfu 보안공학연구지원센터 2016 International Journal of Multimedia and Ubiquitous Vol.11 No.2

Based on the concentrated parameters theory, a 7-freedom coupled vibration dynamic model of the spiral bevel gear transmission system is established, which includes transmission error, time-varying mesh stiffness and the tooth backlash clearance. In the model, the axial vibration, the torsion oscillation of the gear pair aroused by tooth meshing force and the lateral oscillations resulting from flexional deformation of the gear shaft are taken into account. The mesh stiffness fluctuation is developed as 5-order Fourier series and the tooth backlash clearance is fitted by 7-order polynomial function. Through the Gear method, the dynamic response of the system is obtained, and the vibration characteristics are analyzed.

Effect of phosphate on peroxymonosulfate activation: Kinetics, mechanism and implication to in-situ chemical oxidation for water decontamination

Min Tang,Yulin Huang,Na Tian,Yulun Nie 대한환경공학회 2024 Environmental Engineering Research Vol.29 No.2

A symmetric finite volume element scheme on tetrahedron grids

Cunyun Nie,Min Tan 대한수학회 2012 대한수학회지 Vol.49 No.4

We construct a symmetric finite volume element (SFVE) scheme for a self-adjoint elliptic problem on tetrahedron grids and prove that our new scheme has optimal convergent order for the solution and has superconvergent order for the flux when grids are quasi-uniform and regular. The symmetry of our scheme is helpful to solve efficiently the corresponding discrete system. Numerical experiments are carried out to confirm the theoretical results.