女仕牌固体饮料对左甲状腺素钠诱导肾阴虚小鼠模型内分泌系统的影响

류석(刘硕),마사요(马思遥),주문이(朱文驿),양가(梁佳),Yujing Shi,Hong Meng 한국피부과학연구원 2020 아시안뷰티화장품학술지 Vol.18 No.2

Purpose: To observe the effects of Nv Shi solid drink on thyroid hormone, sex hormones and cyclic nucleotide of kidney-yin deficiency hyperthyroid mice induced by levothyroxine sodium. Methods: ICR mice were randomly grouped into normal control group, model group, Nv Shi solid drink high dose group, the middle dose group and low dose group, male and female in half. The kidney-yin deficiency mice models were established by intragastric administration of levothyroxine sodium before intragastric administration of corresponding drugs once a day for 10 consecutive days. One day after the last administration, the effects were observed by measuring weight. And tetraiodothyronine (T4), triiodothyronine (T3), thyrotropin (TSH), testosterone (T), estradiol (E2), cyclic adenosine monophosphate (cAMP), cyclic guanosine monophosphate (cGMP) were tested by ELISA method. Results: Compared with the model group, the body weight increased significantly (p<0.05), T3 level decreased significantly in high and medium dose group (p<0.01, p<0.05), and serum T4 and cAMP decreased significantly (p<0.01, p<0.05), TSH increased significantly (p<0.01, p<0.05) in all dose groups, and cAMP/cGMP decreased in the low dose group (p<0.05). The expression of E2 in female decreased significantly in all does groups, the same as the ratio of E2/T in high dose group (p<0.05). There was no significant change in the expression of sex hormones in male in all dose groups (p>0.05). Conclusion: Nv Shi solid drink may improve the kidney-yin deficiency syndrome by adjusting T3, T4, TSH, cAMP and E2 level in the endocrine system of the body and exert the function of nourishing yin and tonifying kidney. 目的: 观察女仕牌固体饮料对左甲状腺素钠致肾阴虚模型小鼠体内甲状腺激素、性激素及环核苷酸的影响。方 法: ICR小鼠随机分为正常组、模型组、女仕牌固体饮料大、中、小剂量组,雌雄各半,采用左甲状腺素钠片灌 胃造成小鼠肾阴虚模型,造模当天灌胃给予样品,每天1次,连续10天。末次给药后1天,观察小鼠体征及体 重,取血,采用Elisa方法检测血清中甲状腺素(thyroxine, T4)、三碘甲状原氨酸(triiodothyronine, T3)、 促甲状腺激素(thyroid-stimulating hormone, TSH)、睾酮(testosterone, T)、雌二醇(estradiol, E2)、 环磷酸腺苷(cyclic Adenosine monophosphate, cAMP)、环磷酸鸟苷(cyclic guanosine monophosphate, cGMP)的表达。结果: 与模型组比较,女仕牌固体饮料大剂量组和中剂量组小鼠体重显著升高(p<0.05);大 剂量和中剂量组T3显著降低(p<0.01,p<0.05),各剂量组血清T4、cAMP均显著降低(p<0.01,p<0.05), TSH均显著升高(p<0.01,p<0.05),小剂量组cAMP/ cGMP比值降低(p<0.05),各剂量组雌鼠E2表达显著 降低,大剂量组雌鼠E2/T比值降低,各剂量组雄鼠性激素表达无显著改变(p>0.05)。结论: 女仕牌固体饮料 可能通过调整机体内神经分泌系统中T3、T4、TSH、cAMP、雌鼠E2这些共同的潜在物质基础,改善HPT轴和 HPG轴功能、平衡环核苷酸系统,从而改善肾阴虚证,发挥滋阴补肾功效。

Edible mayonnaise-like Pickering emulsion stabilized by pea protein isolate microgels: effect of food ingredients in commercial mayonnaise recipe

Meng Shi 한국산업식품공학회 2022 식품과 기계 Vol.19 No.1

Effects of Bedding Material Composition in Deep Litter Systems on Bedding Characteristics and Growth Performance of Limousin Calves

Meng, J.,Shi, F.H.,Meng, Qingxiang,Ren, L.P.,Zhou, Z.M.,Wu, H.,Zhao, L.P. Asian Australasian Association of Animal Productio 2015 Animal Bioscience Vol.28 No.1

The objective of this study was to evaluate the effects of different litter mixture compositions on bedding system temperature, pH and volatile fatty acid and ammonia-N ($NH_3$-N) content, and the serum physico-chemical parameters and growth indices of calves. Thirty-two Limousin calves ($280{\pm}20kg$) were randomly assigned to four groups (n = 8 for each group) according to the bedding system used: i) control with soil only (CTR); ii) mixture with 50% paddy hulls (PH), 30% saw dusts (SD), 10% peat moss (PM) and 10% corn cobs (CC) (TRT1); iii) mixture with 15% PH, 15% SD, 10% PM, 40% CC, and 20% corn stover (CS) (TRT2); iv) mixture with 30% PH, 10% PM, 40% CC, and 20% CS (TRT3). The litter material combinations of different treatments were based on the cost of bedding system materials in China. The cost of four treatments from low to high: Control<TRT1<TRT2<TRT3. The control was no-cost treatment. The diet consisted of 60.8% silage and 39.2% concentrate (dry matter [DM] basis). The $NH_3$-N level (271.83 to 894.72 mg/kg) was lowest for TRT1 (p<0.0001) and highest for TRT2 (p<0.0001). The acetate, propionate and butyrate levels were highest for the control group (p<0.0001). In all the groups, the pH value (6.90 to 9.09) increased at the beginning and later remained stable at below 9.09. The temperature of deep litter increased at the first week and reached the maximum ($42.1^{\circ}C$) on day 38. 3,5,3'-Triiodothyronine ($T_3$) levels in the TRT1 group animals (p<0.0001) were lower than those in the control and TRT2 animals. 3,5,3',5'-Tetraiodothyronine ($T_4$) in the TRT1 group (p = 0.006) was lower than that in the other treatment groups. Cortisol (COR) in the control and TRT1 group was lower (p<0.0001) than that in the TRT2 and TRT3 groups. Corticosterone (CORt) in the control group was higher (p<0.0001) than that in the treatment groups. The findings indicate that the deep litter bedding systems provided better conditions for animal health and growth performance compared with the control system. Furthermore, the litter composition of TRT1 was found to be optimal among the three treatment groups.

Rice Husk as a Food Carrier Forselective Enrichment of Tea Catechins

Meng Shi,Long-Yue Huang,Rong-Rong Han,Qiao Lin, Jian-Hui Ye,Yue-Rong Liang,Jian-Hui Ye 한국차학회 2015 한국차학회지 Vol.- No.S

The potentials of rice husk, oat bran and soya milk cake as food carriers for tea catechins(TC) were studied. Adsorption properties, contact time and temperature effects and the concentration of tea catechins, as well as digestive stability were investigated in this paper. The result showed that the adsorption capacity of rice husk for tea catechins was 32.04 mgg-1 which was 28.1 % and 17.7 % higher than those of oat bran and soya milk cake and no obvious adsorbability to caffeine was observed for these materials. The selectivity coefficient K of EGCg to TC for rice husk was 4.44 in comparison with 1.87 and 1.96 of oat husk and soya milk cake. This guarantees rice husk a selective food carrier to tea catechins with enrichment of EGCg. Also, 20 min contact time and tea extract concentration above 1.75 g L-1 are recommended in order to obtain a high loading amount of tea catechins onto rice husk, and low temperature is favorable for the adsorption processof tea catechins onto rice husk. Digestive study showed that combination of tea catechins with rice husk was propitious to increase the digestive stability of tea catechins in simulated gastrointestinal fluids. Thus, rice husk is a promising food carrier for tea catechins. Further studies would be focusing on the identification and separation of the functional constituent of rice husk and its interaction mechanism with tea catechins.

Analysis of Operation Characteristics of a Diesel Generator Set with Constant‑Power Pulsed Load

Meng Shi,Puyu Wang,Jinquan Wang,Haichao Li,Ye Xu,Kefeng Huang,Jianke Li 대한전기학회 2019 Journal of Electrical Engineering & Technology Vol.14 No.6

To improve the power quality of a diesel generator set with a pulsed load in a microgrid, this paper studied the operation characteristics of the system and proposed pulsed load conditions for an actual project based on test data. For a low average pulse power and a high peak power, a new mathematical model of the pulsed load in diferent operation modes was proposed. Then, a simulation model of the microgrid was established in MATLAB/Simulink, and the operation of the system was studied considering changes in various pulsed load parameters (the peak power Pk, output duty cycle D and switching period Ts). It was verifed by tests that the simulation model is efective and can refect the operation of the system. Based on the simulation model, the operation characteristics of the system with a bivariate pulsed load were studied, and qualifcation conditions for the diesel generator set in an actual project were proposed.

Determination and prediction of the digestible and metabolizable energy contents of corn germ meal in growing pigs

Meng Shi,Zhaoyu Liu,Hong Liang Wang,Chuanxin Shi,Ling Liu,Junjun Wang,Defa Li,Shuai Zhang 아세아·태평양축산학회 2019 Animal Bioscience Vol.32 No.3

Objective: This experiment was conducted to determine the chemical composition, digestible energy (DE) and metabolizable energy (ME) contents of corn germ meals (CGM) and to develop equations to predict the corresponding energy contents based on the chemical characteristics of individual CGM. Methods: Sixty-six barrows (initial body weight = 51.3±4.6 kg) were allotted to 11 diets including a basal diet and 10 CGM test diets in a completely randomized design. In the test diets, CGM was included in replacement of 30% of the energy-providing ingredients in the basal diet, resulting in a final inclusion rate of 29.1%. Each diet was fed to 6 barrows housed in individual metabolism crates for a 7-d acclimation period followed by a 5-d total but separate collection of feces and urine. Results: Considerable variation was observed in acid-hydrolyzed ether extract, ether extract, ash, calcium (Ca) and total phosphorus contents among the CGM samples. On dry matter (DM) basis, the DE and ME contents of the CGM ranged from 10.22 to 15.83 MJ/kg and from 9.94 to 15.43 MJ/kg, respectively. The acid detergent fiber (ADF) contents were negatively correlated with the DE and ME contents of CGM samples. The best-fit prediction equations for the DE and ME values (MJ/kg DM) of the 10 CGM were: DE = 26.85–0.28 insoluble dietary fiber (%)–17.79 Ca (%); ME = 21.05–0.43 ADF (%)–11.40 Ca (%). Conclusion: The chemical compositions of CGM vary depending on sources, particularly in ether extract and Ca. The DE and ME values of CGM can be predicted based on their chemical composition in growing pigs.

Effects of Bedding Material Composition in Deep Litter Systems on Bedding Characteristics and Growth Performance of Limousin Calves

J. Meng,F.H. Shi,Q.X. Meng,L.P. Ren,Z.M. Zhou,H. Wu,L.P. Zhao 아세아·태평양축산학회 2015 Animal Bioscience Vol.28 No.1

The objective of this study was to evaluate the effects of different litter mixture compositions on bedding system temperature, pH and volatile fatty acid and ammonia-N (NH3-N) content, and the serum physico-chemical parameters and growth indices of calves. Thirty-two Limousin calves (280±20 kg) were randomly assigned to four groups (n = 8 for each group) according to the bedding system used: i) control with soil only (CTR); ii) mixture with 50% paddy hulls (PH), 30% saw dusts (SD), 10% peat moss (PM) and 10% corn cobs (CC) (TRT1); iii) mixture with 15% PH, 15% SD, 10% PM, 40% CC, and 20% corn stover (CS) (TRT2); iv) mixture with 30% PH, 10% PM, 40% CC, and 20% CS (TRT3). The litter material combinations of different treatments were based on the cost of bedding system materials in China. The cost of four treatments from low to high: Control<TRT1<TRT2<TRT3. The control was no-cost treatment. The diet consisted of 60.8% silage and 39.2% concentrate (dry matter [DM] basis). The NH3-N level (271.83 to 894.72 mg/kg) was lowest for TRT1 (p<0.0001) and highest for TRT2 (p<0.0001). The acetate, propionate and butyrate levels were highest for the control group (p<0.0001). In all the groups, the pH value (6.90 to 9.09) increased at the beginning and later remained stable at below 9.09. The temperature of deep litter increased at the first week and reached the maximum (42.1°C) on day 38. 3,5,3′-Triiodothyronine (T3) levels in the TRT1 group animals (p<0.0001) were lower than those in the control and TRT2 animals. 3,5,3′,5′-Tetraiodothyronine (T4) in the TRT1 group (p = 0.006) was lower than that in the other treatment groups. Cortisol (COR) in the control and TRT1 group was lower (p<0.0001) than that in the TRT2 and TRT3 groups. Corticosterone (CORt) in the control group was higher (p<0.0001) than that in the treatment groups. The findings indicate that the deep litter bedding systems provided better conditions for animal health and growth performance compared with the control system. Furthermore, the litter composition of TRT1 was found to be optimal among the three treatment groups.

Soluble Epoxide Hydrolase Inhibitory and Anti-inflammatory Components from the Leaves of <i>Eucommia ulmoides</i> Oliver (Duzhong)

Bai, Meng-Meng,Shi, Wei,Tian, Jun-Mian,Lei, Ming,Kim, Jang Hoon,Sun, Ya Nan,Kim, Young Ho,Gao, Jin-Ming American Chemical Society 2015 Journal of agricultural and food chemistry Vol.63 No.8

<P><I>Eucommia ulmoides</I> leaves have been used as a functional food and drink in China. The purpose of this study was to identify the bioactive constituents with soluble epoxide hydrolase (sEH) inhibitory activity and anti-inflammatory properties. Twenty-seven known compounds (<B>1</B>–<B>27</B>) were isolated from the leaves of <I>E. ulmoides</I> Oliver, and their structures were identified by NMR and ESIMS analysis; three of these, 2,5-dimethoxy-3-glucopyranosyl cinnamic alcohol (<B>11</B>), foliasalacioside E<SUB>2</SUB> (<B>26</B>), and icariside F<SUB>2</SUB> (<B>27</B>), were obtained from this plant for the first time. Compounds <B>1</B>–<B>7</B> exhibited soluble epoxide hydrolase (sEH) inhibitory activity at 100 μM; among them, quercetin (<B>1</B>) and kaempferol (<B>5</B>) displayed potential activities with IC<SUB>50</SUB> values of 22.5 ± 0.9 and 31.3 ± 2.6 μM, respectively, with noncompetitive inhibition mode. Nuclear factor kappa B (NF-κB) inhibitory activity of the isolated compounds was evaluated by the NF-κB liciferase assay in HepG2 cells. Compounds <B>1</B>, <B>9</B>, <B>20</B>, and <B>27</B> displayed potent NF-κB inhibitory effects, with IC<SUB>50</SUB> values of 15.14 ± 2.29, 15.23 ± 2.34, 16.88 ± 2.17, and 16.25 ± 2.19 μM, respectively, whereas other compounds showed weak inhibition of NF-κB transcriptional activity ranging from 17.54 to 92.6 μM. A structure–activity relationship of flavonoids <B>1</B>–<B>9</B> was also discussed. The results obtained in this work might contribute to the understanding of pharmacological activities of <I>E. ulmoides</I> leaves and further investigation on its potential application values for food and drug.</P><P><B>Graphic Abstract</B> <IMG SRC='http://pubs.acs.org/appl/literatum/publisher/achs/journals/content/jafcau/2015/jafcau.2015.63.issue-8/acs.jafc.5b00055/production/images/medium/jf-2015-00055j_0004.gif'></P><P><A href='http://pubs.acs.org/doi/suppl/10.1021/jf5b00055'>ACS Electronic Supporting Info</A></P>

Edible mayonnaise-like Pickering emulsion stabilized by pea protein isolate microgels: effect of food ingredients in commercial mayonnaise recipe

Dr. Shi Meng 한국산업식품공학회 2022 학술대회 및 심포지엄 Vol.2022 No.11

Effect of Sophora flavescens on the pharmacokinetics of carbamazepine in rats

Lei Shi,Xue-Liang Dang,Xin-You Liu,Hua-Mei Wei,Meng-Meng Yang,Yan Zhang 대한약학회 2014 Archives of Pharmacal Research Vol.37 No.12

Carbamazepine (CBZ), an antiepilepticwith narrow therapeutic window, is a substrate ofCYP 3A4 which metabolizes CBZ to carbamazepine-10,11-epoxide (CBZE). CBZE is an active and toxicitymetabolite, and it is a substrate of MRP-2. UsingCBZ for a long time can cause hepatic injury. Sophora flavescens (SF) is a medicinal herb used forthe protected hepatic injury. This study investigatedthe acute and chronic effects of SF on the pharmacokineticsof CBZ in rats. The concentrations of CBZand CBZE in plasma and tissues were determined byHPLC method. The results showed that SF whichsignificantly decreased the AUC0-t of CBZ, increasedCBZE conversely. Tissue analysis showed that theconcentrations of CBZ and CBZE in brain and liverwere decreased by SF. In addition, the distribution ofCBZE in kidney was reduced significantly, whichinfluenced the CBZE excretion and increased the drugtoxic potentially. Results in the current study suggestthat patients using CBZ might be cautioned in the useof SF extract or Sophora-derived products. Meanwhile,patients receiving drugs which are substrates ofCYP 3A4 and/or MRP-2 should be advised of thepotential herb–drug interaction to reduce the risk oftherapeutic failure or increased toxicity of conventionaldrug therapy.