Effects of Allogeneic Blood Transfusion in Patients with Stage II Colon Cancer

Meng, Jin,Lu, Xiao-Bo,Tang, Yuan-Xin,Sun, Gong-Ping,Li, Xin,Yan, Yi-Fei,Liang, Gao-Feng,Ma, Si-Ping,Li, Xiao-Xia Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.1

The aim of the present study was to determine whether allogeneic red blood cell transfusions showed a deleterious effect and what might be preoperative risk factors for blood transfusion in patients with TNM stage II colon cancer. Total 470 patients who fulfilled inclusion criteria were selected for a further 10-year follow-up study. We found that there were statistical significance between non-transfused and transfused group in mortality (P=0.018), local recurrence (P=0.000) and distant metastasis (P=0.040). Local recurrence and distant metastasis between 1 to 3 units and more than 3 units group did not show any significant differences. There was no difference in survival rate between non-transfused and 1 to 3 units group (log rank=0.031, P=0.860). The difference between different blood transfusion volume in transfused patients was found (78.77% vs 63.83%, P=0.006). Meanwhile, the significant difference of survival rate was existed between non-transfused group and more than 3 units group (84.83% vs 63.83%, P=0.002 ). Univariate analysis showed the following 3 variables to be associated with an increased risk of allogeneic blood transfusions: preoperative CEA level (P<0.05), location of tumor (P<0.01) and diameter of tumor (P<0.01). Multivariate analysis revealed that location of tumor and diameter of tumor are two independent factors for requirement of perioperative transfusions. Therefore, allogeneic transfusion increase the postoperative tumor mortality, local recurrence and distant metastasis in patients with stage II colon cancer. The postoperative tumor mortality, local recurrence and distant metastasis were not associated with the blood transfusion volume. The blood transfusion volume was associated with the survival rate. Location of tumor and diameter of tumor were the independent preoperative risk factors for blood transfusion.

Heat-Shock Protein 70 as a Tumor Antigen for in vitro Dendritic Cell Pulsing in Renal Cell Carcinoma Cases

Meng, Fan-Dong,Sui, Cheng-Guang,Tian, Xin,Li, Yan,Yang, Chun-Ming,Ma, Ping,Liu, Yun-Peng,Jiang, You-Hong Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.20

Immunological functions of heat shock proteins (HSPs) have long been recognized. In this study we aimed to efficiently purify HSP70 from renal cell carcinoma and test it as a tumor antigen for pulsing dendritic cells in vitro. HSP70 was purified from renal cell carcinoma specimens by serial column chromatography on Con A-sepharose, PD-10, ADP-agarose and DEAE-cellulose, and finally subjected to fast protein liquid chromatography (FPLC). Dendritic cells derived from the adherent fraction of peripheral blood mononuclear cells were cultured in the presence of IL-4 and GM-CSF and exposed to tumor HSP70. After 24 hours, dendritic cells were phenotypically characterized by flow cytometry. T cells obtained from the non-adherent fraction of peripheral blood mononuclear cells were then co-cultured with HSP70-pulsed dendritic cells and after 3 days T cell cytotoxicity towards primary cultured renal cell carcinoma cells was examined by Cell Counting Kit-8 assay. Dendritic cells pulsed in vitro with tumor-derived HSP70 expressed higher levels of CD83, CD80, CD86 and HLA-DR maturation markers than those pulsed with tumor cell lysate and comparable to that of dendritic cells pulsed with tumor cell lysate plus TNF-${\alpha}$. Concomitantly, cytotoxic T-lymphocytes induced by HSP70-pulsed dendritic cells presented the highest cytotoxic activity. There were no significant differences when using homologous or autologous HSP70 as the tumor antigen. HSP70 can be efficiently purified by chromatography and induces in vitro dendritic cell maturation in the absence of TNF-${\alpha}$. Conspecific HSP70 may effectively be used as a tumor antigen to pulse dendritic cells in vitro.

The Cancer/Testis Antigen CT45A1 Promotes Transcription of Oncogenic Sulfatase-2 Gene in Breast Cancer Cells and Is Sensible Targets for Cancer Therapy

Ping Yang,Yingnan Qiao,Huaidong Liao,Yizheng Huang,Mei Meng,Yu Chen,Quansheng Zhou 한국유방암학회 2023 Journal of breast cancer Vol.26 No.2

Purpose: Invasive breast carcinomas (BRCAs) are highly lethal. The molecular mechanisms underlying progression of invasive BRCAs are unclear, and effective therapies are highly desired. The cancer-testis antigen CT45A1 promotes overexpression of pro-metastatic sulfatase-2 (SULF2) and breast cancer metastasis to the lungs, but its mechanisms are largely unknown. In this study, we aimed to elucidate the mechanism of CT45A1-induced SULF2 overexpression and provide evidence for targeting CT45A1 and SULF2 for breast cancer therapy. Methods: The effect of CT45A1 on SULF2 expression was assessed using reverse transcription polymerase chain reaction and western blot. The mechanism of CT45A1-induced SULF2 gene transcription was studied using protein-DNA binding assay and a luciferase activity reporter system. The interaction between CT45A1 and SP1 proteins was assessed using immunoprecipitation and western blot. Additionally, the suppression of breast cancer cell motility by SP1 and SULF2 inhibitors was measured using cell migration and invasion assays. Results: CT45A1 and SULF2 are aberrantly overexpressed in patients with BRCA; importantly, overexpression of CT45A1 is closely associated with poor prognosis. Mechanistically, gene promoter demethylation results in overexpression of both CT45A1 and SULF2. CT45A1 binds directly to the core sequence GCCCCC in the promoter region of SULF2 gene and activates the promoter. Additionally, CT45A1 interacts with the oncogenic master transcription factor SP1 to drive SULF2 gene transcription. Interestingly, SP1 and SULF2 inhibitors suppress breast cancer cell migration, invasion, and tumorigenicity. Conclusion: Overexpression of CT45A1 is associated with poor prognosis in patients with BRCA. CT45A1 promotes SULF2 overexpression by activating the promoter and interacting with SP1. Additionally, SP1 and SULF2 inhibitors suppress breast cancer cell migration, invasion, and tumorigenesis. Our findings provide new insight into the mechanisms of breast cancer metastasis and highlight CT45A1 and SULF2 as sensible targets for developing novel therapeutics against metastatic breast cancer.

A Diffusion Filter for Discontinuous Surface Measured by High Definition Metrology

Meng Wang,Yi-Ping Shao,Shi-Chang Du,Li-feng Xi 한국정밀공학회 2015 International Journal of Precision Engineering and Vol. No.

High definition metrology can measure three-dimensional surface topography of discontinuous surfaces that have holes and grooves with large field of field and high lateral resolution. However, when separating discontinuous surfaces in to various scale-limited surface such as form, waviness and roughness, filtering techniques such as Gaussian filters cause “boundary distortion problems. This is because the discontinuous surfaces do not have large enough continuous evaluation area for Gaussian filtering. Therefore, this research proposes a modified anisotropic diffusion filter, which is formulated in terms of heat diffusion, to separate discontinuous surfaces into various scale-limited surfaces. An edge detector is proposed to ensure that the diffusion process only takes place inside the surface but not blur the surface boundaries. In addition, the diffusion time is specified considering the linkage with the cutoff wavelength of Gaussian filters. The performance of the proposed filter is validated by simulated and practical discontinuous surfaces. Moreover, the three dimensional areal surface texture features specified in ISO 25178 can be evaluated from the filtered scale-limited surfaces. Results have demonstrated that the proposed approach is an effective tool for separating and evaluating areal surface texture for discontinuous surfaces regardless of the continuity of the evaluation surface.

Differential equations related to family [문자]

Ping Li,Yong Meng 대한수학회 2011 대한수학회보 Vol.48 No.2

Let h be a meromorphic function with few poles and zeros. By Nevanlinna's value distribution theory we prove some new properties on the polynomials in h with the coefficients being small functions of h. We prove that if f is a meromorphic function and if f^m is identically a polynomial in h with the constant term not vanish identically, then f is a polynomial in h. As an application, we are able to find the entire solutions of the differential equation of the type f^n+P(f)=be^sz+Q(e^z), where P(f) is a differential polynomial in f of degree at most n-1, and Q(e^z) is a polynomial in e^z of degree k ≤max{n-1,s(n-1)/n} with small functions of e^z as its coefficients.

Synthesis and Electrochemical Characteristics of Fe-P Alloy Prepared by Electrothermal Reduction Method

Meng Zhao,Gui-Xin Wang,Xiu-Li Li,Rui Liu,Kang-Ping Yan 대한금속·재료학회 2010 METALS AND MATERIALS International Vol.16 No.6

Microscale Fe-P alloy was prepared using Ca3(PO4)2 and Fe2O3 in an electrothermal reduction process, and the electrochemical performance was investigated in detail. The initial discharge capacity could reach ~566.8mAh/g at 0.3 C rate, and the fade trend was so slight that the normalized capacities from 1.0 to 2.0 C rate were adjacent. The energy density and the power density could reach ~1133.6 Wh/kg and ~912.4 W/kg,respectively. The rate capability and the cycle performance were comparable to those of the Fe-P alloy synthesized using zerovalent iron and phosphorus. At 0.5 mV/s scan rate, the oxidation peak and the reduction peak for the reaction of lithium ions with P were positioned at ~1.3 and ~0.5 V, respectively. The reaction occurred under diffusion control, and the lithium ion diffusion efficient was ~1.5×10−9 cm2/s.

Protein Kinase C Mediates the Corticosterone-induced Sensitization of Dorsal Root Ganglion Neurons Innervating the Rat Stomach

( Meng Li ),( Lu Xue ),( Hong-yan Zhu ),( Hongjun Wang ),( Xue Xu ),( Ping-an Zhang ),( Geping Wu ),( Guang-yin Xu ) 대한소화기기능성질환·운동학회 2017 Journal of Neurogastroenterology and Motility (JNM Vol.23 No.3

Background/Aims Gastric hypersensitivity contributes to abdominal pain in patients with functional dyspepsia. Recent studies showed that hormones induced by stress are correlated with visceral hypersensitivity. However, the precise mechanisms underlying gastric hypersensitivity remain largely unknown. The aim of the present study was designed to investigate the roles of corticosterone (CORT) on excitability of dorsal root ganglion (DRG) neurons innervating the stomach. Methods DRG neurons innervating the stomach were labeled by DiI injection into the stomach wall. Patch clamp recordings were employed to examine neural excitability and voltage-gated sodium channel currents. Electromyograph technique was used to determine the responses of neck muscles to gastric distension. Results Incubation of acutely isolated DRG neurons with CORT significantly depolarized action potential threshold and enhanced the number of action potentials induced by current stimulation of the neuron. Under voltage-clamp mode, incubation of CORT enhanced voltage-gated sodium current density of the recorded neurons. Pre-incubation of GF109203X, an inhibitor of protein kinase C, blocked the CORT-induced hyperexcitability and potentiation of sodium currents. However, pre-incubation of H-89, an inhibitor of protein kinase A, did not alter the sodium current density. More importantly, intraperitoneal injection of CORT produced gastric hypersensitivity of healthy rats, which was blocked by pre-administration of GF109203X but not H-89. Conclusions Our data strongly suggest that CORT rapidly enhanced neuronal excitability and sodium channel functions, which is most likely mediated by protein kinase C but not protein kinase A signaling pathway in DRG neurons innervating the stomach, thus underlying the gastric hypersensitivity induced by CORT injection. (J Neurogastroenterol Motil 2017;23:464-476)

The Effects of Nurse Retention by a Supportive Group in New Graduate Nurses

Meng-Ru Tseng,H L Huang,Tsui-Ping Chu,Bih-O Lee 한국간호과학회 2015 한국간호과학회 학술대회 Vol.2015 No.10

A MobileNet-SSD Model with FPN for Waste Detection

Meng Jing,Jiang Ping,Wang Jianmin,Wang Kai 대한전기학회 2022 Journal of Electrical Engineering & Technology Vol.17 No.2

Over the past few years, as environmental problems have been progressively deteriorating, waste classifi cation has also become a research hotspot. As an algorithm with better detection accuracy and speed, SSD (Single Shot MultiBox Detector) has made great progress in many aspects. However, it can’t achieve a good detection eff ect for small objects because it does not make full use of high-level semantic information. In this paper, we propose a MobileNet-SSD model with FPN to solve the problem of waste detection, which can reduce parameters, narrow internal space and improve performance for small objects compared with SSD model. Besides, Focal Loss is adopted to reduce the imbalance between foreground and background samples to enhance detector eff ect. To verify the eff ectiveness of the approach proposed here, a dataset for waste detection is collected and annotated. As revealed from the experimental results, the mAP of our method is 93.63% and the speed is 102 FPS, which can beat other methods.

Study on Nano-Metal Oxide and Carbon Nanotube Composites on Thermal Decomposition of Potassium Perchlorate

Ping-An Liu,Meng-Jun Wang,Lu Wang,Jun Wang,Ting Wang 대한화학회 2019 Bulletin of the Korean Chemical Society Vol.40 No.4

In this study, novel carbon nanotube (CNT) and transition-metal oxides (TMOs) nanoparticle catalysts were prepared and their effects on the thermal decomposition of potassium perchlorate (KP) were investigated. Nanocatalysts have been widely studied because of their excellent properties, such as the high surface energies of nanoparticles and their ease of agglomeration adversely affect their catalytic performances. Good dispersion is the key to good nanocatalytic activity. The composite catalysts were prepared by loading a nanocatalyst on CNTs; this improves nanoparticle dispersion, promotes electron transfer during the reaction, and increases the catalytic effect. Different types of CNT-based composite catalysts and their corresponding single nanocatalysts were each added to KP. The effects of the catalysts on the thermal decomposition of KP were studied using thermogravimetric analysis (TGA) and differential scanning calorimetry (DSC). The activity of each catalyst was evaluated on the basis of the changes in the thermal decomposition peaks and the apparent decomposition heats. The results show that the activity of a composite catalyst in KP thermal decomposition is much higher than that of its corresponding single nanocatalyst.