http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Improved Micropropagation of Root Chicory, Cichorium intybus L. var. sativus.
Lim,Jung-Dae,Yang,Deok-Chun,Lee,Hyeon-Yong,Kim,Jong-Dai,Lee,Jin-Ha,Sung,Eun-Soo,Yu Chang-Yeon 한국자원식물학회 2004 Plant Resources Vol.7 No.1
The establishment of an efficient protocol for plant regeneration and micropropagation from leaf explant cultures of Chicory, Cichorium intybus L. var. sativus. is reported. Callus formation rate appeared 100% from explant in all growth regulators, but calli formed in the prensence of naphthaleneacetic acid (NAA) were appeared very compact and non-embryogenic state. The regenerated shoots were obtained from leaf explant cultures on solid MS medium containing different concentrations of cytokinins and auxin. The highest number of shoots (5.7) per explant and shoot growth (2.8cm) was obtained on MS medium containing 0.1 mg BAP L^-1 and 0.1 mg NAA L^-1. Indole acetic acid was the most suitable auxin for root formation among three auxins tested. 2,4-D had no effect on shoot and root formation.
Jin‑Sung Park,Joo‑Il Kim,Hyun‑Jin Lim,Soo‑Kyung Ryu,Euna Kwon,Kang‑Min Han,Ki‑Taek Nam,Han‑Woong Lee,Byeong‑Cheol Kang 한국유전학회 2020 Genes & Genomics Vol.42 No.9
Background p19arf, primarily known as a tumor suppressor, has also been reported to play an essential role in normal development of mouse eyes. Consistently, lack of p19arf has been associated with ocular defects, but the mixed background of the knockout (KO) mouse strain used raised a concern on the accuracy of the phenotypes observed in association with the targeted gene due to genetic heterogeneity. Object We carried out a study to investigate into the efect of genetic background on the manifestation of p19arf KO associated phenotypes. Methods We characterized the phenotypes of novel p19arf KO mouse lines generated in FVB/N and C57BL/6J using a transcription activator-like efector nuclease (TALEN) system in comparison to the reported phenotypes of three other p19arf-defcient mouse lines generated using homologous recombination. Results Ninety-fve percent of FVB/N-p19arf KO mice showed ocular opacity from week 4 after birth which worsened rapidly until week 6, while such abnormality was absent in C57BL/6J-p19arf KO mice up to the age of 26 weeks. Histopathological analysis revealed retrolental masses and dysplasia in the retinal layer in FVB/N-p19arf KO mice from week 4. Besides these, both strains developed normally from birth to week 26 without increased tumorigenesis except for a subcutaneous tumor found in a C57BL/6J-p19arf KO mouse. Conclusion Our fndings demonstrated surprisingly variable manifestation of p19arf-linked phenotypes between FVB/N and C57BL/6J mice, and furthermore between our mouse lines and the established lines, indicating a critical impact of genetic background on functional study of genes using gene targeting strategies in mice.
Dae-Weon Lee,Il Sung Moon,강재순,Hyun Jung Choe,최재영,제연호,이시혁,Hye Rim Han,Kook-Jin Lim6,Young Ho Koh 한국응용곤충학회 2007 Journal of Asia-Pacific Entomology Vol.10 No.2
Bursaphelenchus xylophilus has been recognized as a causal pathogen of pine wilt disease (PWD). In order to identify relevant molecular biomarkers, we selected a variable region of endo-β-1,4-glucanase (β14-gcn) which was cloned from the cDNA of B. xylophilus and B. mucronatus. We then identified three novel β14-gcn isoforms: Bm-β14-gcn and Bm-β14-gcni from B. mucronatus, and Bx-β14-gcn from B. xylophilus. Bm-β14-gcn and Bx-β14-gcn were found to be identical, whereas Bm-β14-gcni was unique to B. mucronatus. These isoforms are Bursaphelenchus-specific and may be employed as molecular markers for the diagnosis of PWD. Our phylogenetic analysis showed that these β14-gcns from Bursaphelenchus species were associated most closely with fungal β14-gcns.
Park, Jinbong,Jeon, Yong-Deok,Kim, Hye-Lin,Lim, Hara,Jung, Yunu,Youn, Dong-Hyun,Jeong, Mi-Young,Kim, Hyun-Ju,Kim, Sung-Hoon,Kim, Su-Jin,Hong, Seung-Heon,Um, Jae-Young Hindawi Publishing Corporation 2013 Evidence-based Complementary and Alternative Medic Vol.2013 No.-
<P>Obesity has become a major health threat in developed countries. However, current medications for obesity are limited because of their adverse effects. Interest in natural products for the treatment of obesity is thus rapidly growing. Korean Medicine (KM) is characterized by the wide use of herbal formulas. However, the combination rule of herbal formulas in KM lacks experimental evidence. According to <I>Shennong's Classic of Materia Medica</I>, the earliest book of herbal medicine, <I>Veratrum nigrum</I> (VN) has antagonistic features against <I>Panax ginseng</I> (PG), and the PG-VN pair is strictly forbidden. In this study, we have shown the effects of PG, VN, and their combination on obesity in high-fat (HF) diet-induced obese mice and in 3T3-L1 cells. PG, VN, and PG-VN combination significantly reduced weight gain and the fat pad weight in HF diet-induced obese mice. They also significantly decreased lipid accumulation and the expressions of two major adipogenesis factors, PPAR<I><I>γ</I></I> and C/EBP<I><I>α</I></I>, in 3T3-L1 cells. In addition, the PG-VN combination had synergistic effects compared with the mixture of extracts of PG and VN on inhibition of PPAR<I><I>γ</I></I> and C/EBP<I><I>α</I></I> expressions at lower doses. These results indicate a new potential anti-obese pharmacotherapy and also provide scientific evidence supporting the usage of herbal combinations instead of mixtures in KM.</P>
Lim, Jung Yeon,Park, Sang In,Kim, Seong Muk,Jun, Jin Ae,Oh, Ji Hyeon,Ryu, Chung Hun,Jeong, Chang Hyun,Park, Sun Hwa,Park, Soon A,Oh, Wonil,Chang, Jong Wook,Jeun, Sin-Soo Pergamon Press ; Elsevier Science Ltd ; Elsevier S 2011 CELL TRANSPLANTATION Vol.20 No.11
<P>The ability of mesenchymal stem cells (MSCs) to differentiate into neural cells makes them potential replacement therapeutic candidates in neurological diseases. Presently, overexpression of brain-derived neurotrophic factor (BDNF), which is crucial in the regulation of neural progenitor cell differentiation and maturation during development, was sufficient to convert the mesodermal cell fate of human umbilical cord blood-derived MSCs (hUCB-MSCs) into a neuronal fate in culture, in the absence of specialized induction chemicals. BDNF overexpressing hUCB-MSCs (MSCs-BDNF) yielded an increased number of neuron-like cells and, surprisingly, increased the expression of neuronal phenotype markers in a time-dependent manner compared with control hUCB-MSCs. In addition, MSCs-BDNF exhibited a decreased labeling for MSCs-related antigens such as CD44, CD73, and CD90, and decreased potential to differentiate into mesodermal lineages. Phosphorylation of the receptor tyrosine kinase B (TrkB), which is a receptor of BDNF, was increased significantly in MSC-BDNF. BDNF overexpression also increased the phosphorylation of β-catenin and extracellular signal-regulated kinases (ERKs). Inhibition of TrkB availability by treatment with the TrkB-specific inhibitor K252a blocked the BDNF-stimulated phosphorylation of β-catenin and ERKs, indicating the involvement of both the β-catenin and ERKs signals in the BDNF-stimulated and TrkB-mediated neural differentiation of hUCB-MSCs. Reduction of β-catenin availability using small interfering RNA-mediated gene silencing inhibited ERKs phosphorylation. However, β-catenin activation was maintained. In addition, inhibition of β-catenin and ERKs expression levels abrogated the BDNF-stimulated upregulation of neuronal phenotype markers. Furthermore, MSC-BDNF survived and migrated more extensively when grafted into the lateral ventricles of neonatal mouse brain, and differentiated significantly into neurons in the olfactory bulb and periventricular astrocytes. These results indicate that BDNF induces the neural differentiation of hUCB-MSCs in culture via the TrkB-mediated phosphorylation of ERKs and β-catenin and following transplantation into the developing brain.</P>
( Jin Kim ),( Jung Eun Kim ),( Ji Young Lim ),( Sang Hee Yoon ),( Sun Joo Lee ),( Soo Nyung Kim ),( Soon Beom Kang ),( Seung Hyuk Shim ) 대한산부인과학회 2012 대한산부인과학회 학술대회 Vol.99 No.-
To determine the relationship between preoperative hypoalbuminemia and the development of complications after gynecological cancer surgery, as well as postoperative bowel function and hospital stay. The medical records of 585 patients with elective gynecological cancer surgery at Konkuk University Hospital during 2005 and 2013 were reviewed. The patients had pre-operative serum albumin assessment. Albumin less than 3.5g/dL was defined as hypoalbuminemia. All peri- and postoperative complications within 30 days after surgery, including mortality, complications, time to resumption of normal diet and length of hospital stay, were analyzed. The median age was 47 (range, 21-79) years. Thirty-four patients (17%) had hypoalbuminemia. Hypoalbuminemic patients had significantly higher consumption of alcohol >2 standard drinks per day and higher rate of preoperative systemic infection compared with non-hypoalbuminemic patients (14.8% vs 1.6%; p=0.001, 0% vs 11.1%; p<0.001, respectively). Overall complication rate within 30 days after surgery was 21.9%. Hypoalbuminemic patients were more likely to develop at least 1 postoperative complication compared to non-hypoalbuminemic patients (37% vs 24%; p=0.039). Time to resumption of normal diet in hypoalbuminemic patients was significantly longer than that in non-hypoalbuminemic patients (3.3 vs 2.6 days, p=0.020), whereas length of hospitalization was not significantly different between the two groups. In univariate analysis, hypoalbuminemia, laparotomy (vs laparoscopy) and intraoperative estimated blood loss were risk factors for postoperative complications. In multivariate analysis, after controlling for age, complexity of surgery, cancer site, Charlson morbidity index, smoking, body mass index, operative time, preoperative chemotherapy or radiotherapy, and American Society of Anesthesiology physical status classification scores, hypoalbuminemia (odds ratio 3.219, 95% CI 1.246-8.314; p=0.016) and estimated blood loss (odds ratio 1.001, 95% CI 1.000-1.002; p=0.013) remained as significant risk factors. Preoperative hypoalbuminemia in patients with elective surgery for gynecologic malignancy is an independent predictor of postoperative morbidity. Although the causes of hypoalbuminemia are multifactorial, identification of this subset of patients and preoperative optimization of nutritional status may improve surgical outcomes in this high risk population.
( Jin-sun Lee ),( Gyeong-hwuii Kim ),( Jaegon Kim ),( Tae-hyun Lim ),( Yong Won Yoon ),( Sung-sik Yoon ) 한국미생물 · 생명공학회 2021 Journal of microbiology and biotechnology Vol.31 No.10
Cronobacter sakazakii is an opportunistic pathogenic bacterium found in powdered infant formula and is fatal to neonates. Antibiotic resistance has emerged owing to overuse of antibiotics. Therefore, demand for high-yield bacteriophages as an alternative to antibiotics has increased. Accordingly, we developed a modified mass-production method for bacteriophages by introducing a two-stage self-cycling (TSSC) process, which yielded high-concentration bacteriophage solutions by replenishing the nutritional medium at the beginning of each process, without additional challenge. pH of the culture medium was monitored in real-time during C. sakazakii growth and bacteriophage CS01 propagation, and the changes in various parameters were assessed. The pH of the culture medium dropped to 5.8 when the host bacteria reached the early log phase (OD540 = 0.3). After challenge, it decreased to 4.65 and then recovered to 4.94; therefore, we set the optimum pH to challenge the phage at 5.8 and that to harvest the phage at 4.94. We then compared phage production during the TSSC process in jar-type bioreactors and the batch culture process in shaker flasks. In the same volume of LB medium, the concentration of the phage titer solution obtained with the TSSC process was 24 times higher than that obtained with the batch culture process. Moreover, we stably obtained high concentrations of bacteriophage solutions for three cycles with the TSSC process. Overall, this modified TSSC process could simplify large-scale production of bacteriophage CS01 and reduce the unit cost of phage titer solution. These results could contribute to curing infants infected with antibiotic-resistant C. sakazakii.