팔당호 연안생태계의 수생식물상과 생태적 특성

임용석,마선미,나성태,최홍근,신현철 한국육수학회 2005 생태와 환경 Vol.38 No.1

팔당호 연안대의 수생식물상과 식생을 파악하기 위하여 2003년 4월부터 2004년 4월까지 9곳의 조사 지점을 선정하여 선방형구법을 이용하여 조사하였다. 팔당호 연안대에는 128종류의 관속식물이 생육하고 있는 것으로 조사되었고, 이중 수생식물은 38종류였다. 수생식물의 경우 정수식물이 21종류로 가장 많았고, 침수식물은 8종류 였고, 침수식물 종류수는 이전 조사보다 감소한 것으로 파악되었다. 팔당호 주요 식생은 애기부들, 줄, 갈대가 우점하는 정수식물대로 파악되었고, 특히 두물머리, 광동교 근처에 넓게 정수식물대가 넓게 발달하였다. 귀화식물은 11종류가 분포하고 있었으나, 출현 빈도는 상대적으로 높았다. 팔당호 연안대에 분포하는 식물의 출현 빈도를 조사 지점을 기준으로 살펴보면, 조사 지점당 수생식물은 2.7종류, 습생식물은 2.5종류, 육지식물은 1.8종류로 파악 되었고, 수직대상분포 식생이 팔당호 연안대에서 발견되었다. 이러한 조사 결과는 팔당호 연안대가 수생태계의 전형적인 특성들을 지니고 있는 것으로 풀이된다. To investigate the flora and vegetation structure of vascular plants in the littoral zone of Paldang Reservoir, from April, 2003 to April 2004, nine sampling sites were selected. Along the Paldang Reservoir, 128 taxa, consisted of 51 families and 96 genera, were identified, among them, hydrophytes were confirmed as 38 taxa, which was comprised 29.7% to total taxa, whereas hygrophytes were 44 taxa and terrestrial plants were 46 taxa. Emergent hydrophytes consists of 21 taxa, including Phragmites australis and Typha angustifolia, and next, submerged hydrophytes were 8 taxa. However, the kinds and vegetation area of submerged hydorphytes were reduced compared to previous studies. In the littoral zone of Paldang Reservoir, the aquatic vegetation was widely developed near Dumulmori, Yangsuri, and Kwangdong Bridge, downstream of Kyungancheon. The average number of hydrophyte per sampling sites were 2.7 taxa, whereas hygrophytes were 2.5, and land plants were 1.8. In addition, the hydrophytes in the littoral zone of Paldang Reservoir showed the typical vertical zonation pattern like a natural swamp. These results mean that the littoral zone of Paldang Reservoir has the typical characteristics of aquatic plant ecosystem.

식도를 포함한 전위장관을 침범한 전이성 악성 흑색종 1예

최정영,김준기,신성하,고영춘,조상욱,서강석,강명원,임연근,여향순,김경수 全南大醫大 2001 전남의대학술지 Vol.37 No.1

소아에서 출혈을 동반한 단일 연소성 용종의 내시경적 절제술

김준기,조상욱,신성하,고영춘,최정영,서강석,강명원,임연근,여향순,김경수 全南大醫大 2001 전남의대학술지 Vol.37 No.3

Isolation and Identification of Two Unreported Sordariomycetes Fungi in Korea: Pestalotiopsis clavata and Botryotrichum iranicum

Seong-Keun Lim,Leonid N. Ten,Diane Avalos-Ruiz,류정주,In-Kyu Kang,Seung-Yeol Lee,Hee-Young Jung 한국균학회 2022 韓國菌學會誌 Vol.50 No.3

Two fungal strains, designated KNUF-21-006 and KNUF-21-028, were isolated from soil samples collected from Gyeongbuk Province, Korea. The strain KNUF-21-006 was similar to other Pestalotiopsis species in terms of morphological characteristics, including whitish to pale brown mycelium, conidial shape, and size. The isolate had aerial hyphae that produced black fruiting bodies on the mycelium. The conidia were fusoid to ellipsoid, four-septate, and appendage-bearing. Phylogenetic analysis using the internal transcribed spacer (ITS) region, translation elongation factor 1-alpha (TEF), and β-tubulin (TUB) gene sequences confirmed that the closest relationship of the isolate at the species level was with Pestalotiopsis clavata. The strain KNUF-21-028 exhibits similar morphological characteristics to other Botryotrichum species, including white aerial mycelium with sulcate and irregular margins, conidial shape, and size. The conidia were globose, single, and hyaline. Upon molecular analysis—using the ITS region, large subunit (LSU) rRNA gene, and TUB gene sequences—the fungus was identified as Botryotrichum iranicum. This is the first record of these fungal species in Korea.

Increase of Production Ratio of Pre-selected Superior Dairy Female Offspring by Combination of OPU derived Oocytes and X-bearing Semen

Seong-Su Kim,Byung-Hyun Choi,Kyeong-Lim Lee,Jong-In Jin,Tae-Kwang Suh,Cheol-Ho Son,Chan-Ho Park,Seung-Oh Shin,Kwang-Jin Han,Hyun-Tae Lim,Kyu-Woan Cho,Il-Keun Kong 한국수정란이식학회 2015 한국동물생명공학회지 Vol.30 No.2

This study was designed to evaluate the possibility of increase through dairy female offspring’s ratio by transfer of pre-selected transferrable blastocyst that was produced by pre-selected X-bearing semen with OPU derived oocytes. Elite dairy female cow is demanded strongly compared with male, the so called, farmer wants to produce only an elite female dairy offspring as a candidate female dairy cow for producing milk. In our study, we selected 2 elite dairy bull semen from National Agricultural Cooperative Federation to pre-select X-bearing semen and 5 elite dairy female cows as donor for collecting of OPU derived oocytes. OPU derived embryo production system was carried out an aspiration of immature oocytes from 5 donor cows 2 times per week, total 200 times for 2 to 7 months by an ultrasonographic guided follicular aspiration system and then produced in vitro-produced blastocysts by in vitro maturation, fertilization and culture. Dairy donor semen selected H-319, 320 bull in National Agricultural Cooperative federation was sorted X-bearing semen by flow-cytometer and frozen for using IVF with OPU derived oocytes. Donor cows were selected 5 elite dairy cows from Gyeongju Dairy Cow Community and then disease tests such as 4 kinds of disease before selecting was checked. Oocyte proportion of grade 1 to 3 from total collected oocytes was significantly lower in donor A and B than those in donor C, D and E (82.16 and 70.03% vs. 90.0, 91.78 and 93.57%), respectively (p<0.05). However, number of oocytes per session in donor A, C and E was significantly higher than those in donor B and D (7.77 ± 3.26, 5.85 ± 2.10 and 7.03 ± 2.14 vs. 4.68 ± 2.61 and 5.21 ± 1.97 oocytes), but donor A was significantly higher than donor C (p<0.05). Development to blastocyst in donor B, C and E was significantly higher than those in donor A and D (31.0, 25.0 and 25.0% vs. 14.3 and 4.5%), but donor A was not different in donor C and E (p<0.05). Nine out of 10 blastocysts (90.0%) derived from OPU blastocysts were confirmed male embryos that was induced with Y-bearing semen to confirm sex ratio only. Total 96 blastocysts derived from female bearing semen were transferred into synchronized recipients and then confirmed 42 recipients (43.8%) pregnancy rate, 36 offspring (37.5%) and 91.7% female sex ratio (33 female vs. 3 male offspring). Taken together all data, elite dairy female offspring could be produced effectively by in vitro production system between pre-selected x-bearing semen and OPU derived oocytes that would be influential breeder in the breeding of dairy farm to increase effectively elite dairy offspring ratio as well as net income in the dairy farmer.

Morphological and Phylogenetic Analyses Reveal a New Species of Genus Monochaetia Belonging to the Family Sporocadaceae in Korea

Lim Seong-Keun,Lim Seong-Keun,Hong Soo-Min,Suh Sang Jae,Lee Seung-Yeol,Jung Hee-Young 한국균학회 2023 Mycobiology Vol.51 No.2

The fungal strain belonging to the genus Monochaetia of the family Sporocadaceae was isolated from hairy long-horned toad beetle (Moechotypa diphysis) during the screening of microfungi associated with insects from Gangwon Province, Korea. The strain KNUF-6L2F produced white, light brown to dirty black surface, and olivaceous green colonies with the higher growth, while the closest strain M. ilicis KUMCC 15–0520T were light brown to brown, and M. schimae SAUCC 212201T light brown to brown toward center. The strain KNUF-6L2F produced shorter (5.7–14.0 μm) apical appendages than M. ilicis (6.0–24.0 μm), but similar to M. schimae (7.0–12.5 μm). Three median cells of KNUF-6L2F were light brown to olivaceous green, whereas brown and olivaceous cells were observed from M. ilicis and M. schimae, respectively. And the strain KNUF-6L2F produced larger conidiogenous cells than M. ilicis and M. schimae. Additionally, phylogenetic analyses based on molecular datasets of internal transcribed spacer (ITS) regions, translation elongation factor 1-alpha (TEF1α), and β-tubulin (TUB2) genes corroborated the strain’s originality. Thus, the strain is different from other known Monochaetia species, according to molecular phylogeny and morophology, hence we suggested the new species Monochaetia mediana sp. nov. and provided a descriptive illustration.

Improved Tools for Proteome Application of Basic Seed Proteins in Soybean (Glycine max L. Merr.)

Seong-Woo Cho,Hye-Lim Kim,Keun-Yook Chung,Hong-Sig Kim,Sun-Hee Woo 한국육종학회 2012 한국육종학회 심포지엄 Vol.2012 No.07

We improved the separation of the basic proteins from the soybean cotyledon, Glycine max L. Merr. by searching N-terminal sequences data in proteins isolated by two-dimensional electrophoresis (2-DE). After removed Hexane, proteins were extracted from cotyledon with a urea/Triton/2-mercaptoetanol solution. Using this method, the highly reproducible isoelectric focusing (IEF) can formed with polyacrylamide gels with pH 4.0-9.8. The IEF tube gels were used as the first dimension, and proteins were visualized by second-dimensional gel electrophoresis, and identify a number of soybean cotyledon proteins using mass spectrometry in the proteome analysis. These instruments of 2-DE and IEF tube gels were used 27 cm and investigate under various conditions. The total number of spots and features was obtained by PDQuest software (Bio-Rad). In this experiments performed, the IEF tube gels and instruments afforded good reproducibility in the number of PDQuest-detected spots from gel to gel while IPG offered better reproducibility in the total number of manually detected spots from gel to gel. In conclusion, we have separated of the basic 13 proteins in soybean. The glycinin subunit separations are also considered to play important roles in soybean breeding and biochemical characterization. The improved technique will be useful to dissect the genetic control of glycinin expression in soybean.

Multiplex PCR 을 이용한 요로병원성 대장균의 독성 유전자에 관한 연구

임성준(Seong Joon Lim),김소영(So Young Kim),임인석(In Seok Lim),윤신원(Shin Won Yoon),채수안(Soo Ahn Chae),이동근(Dong Keun Lee),최응상(Eung Sang Choi),유병훈(Byoung Hoon Yoo),송미옥(Mi Ok Song),김원용(Won Yong Kim) 대한신장학회 2002 Kidney Research and Clinical Practice Vol.21 No.4

배 경 : 요로감염을 일으킨 대장균의 분리주에서 독성 유전자를 증명하고 독성유전자의 보유상태와 숙주인자와의 연관성을 파악하여 감염증의 중증도와 재발성을 예측할 수 있는지 알아보고자 하였다. 방 법 : 2001년 4월부터 2001년 6월까지 중앙대학교 용산병원에 요로감염이 의심되어 소변배양을 시행한 환자에서 대장균이 자란 45명 중 임상 기록이 적합한 39명의 소변배양균주에서 DNA를 추출하여 각 DNA에서 요로병원성 대장균의 독성 유전자로 알려진 pap, sfa, afa, cnf, hly 유전자를 multiplex PCR의 방법을 사용하여 입증하였다. 그리고 환자의 임상기록 즉 환자의 나이, 성, 방사선학적 소견, 최종진단, 재발여부, 신경인성 방광 등의 위험요인을 후향적으로 분석하여 독성 유전자와의 연관성을 보았다. 결 과 : 요로감염을 일으킨 실험균주에서 pap+ 균주는 17예, sfa^+는 4예, afa^+는 7예, cnf^+와 hly^+는 각각 6예, 8예였다. 유전자간의 연관성에 있어서 sfa+인 균주는 cnf gene을 함께 보유하는 경우가 많았으며(p<0.001), afa+는 cnf나 hly gene을 함께 보유하는 경우가 많았다(p=0.026, <0.001). cnf^+는 sfa나 afa 또는 hly도 보유하는 경우가 많았다(p<0.001, 0.026, 0.002). 유전자형의 종류는 모두 10개였고 이중 pap^+sfa^-afa^-cnf^-hly^-가 가장 우세하였고(14예 : 36%), 독성 유전자가 없는 pap^-sfa^-afa^-cnf^-hly^-도 12예(31%)였다. 특정 독성 유전자나 유전자형은 남녀에 차이가 없었다. 연령군별로 보았을 때는 1세 이하군에서 pap^-sfa^-afa^+cnf^+hly^+형, 2-15 세 군에서는 pap^-sfa^-afa^-cnf^-hly^-형, 16-40세 군에서는 pap^+sfa^-afa^-cnf^-hly^-형이 많았다. 질환에 따라 특정 독성 유전자를 보유하고 있는 경우나 유전자형은 통계학적으로 의미가 없었다. 재발성 감염증의 경우 초감염보다 독성 유전자의 보유가 통계학적으로 의미있게 더 많거나 적지 않았다. 신경인성 방광이 있는 경우는 sfa+가 의미있게 많았고(p=0.019), 유전자형 pap^+sfa^+afa^-cnf^+hly^-는 모두 신경인성 방광 환자에서 분리된 균주였다(p<0.001). 결 론 : 본 multiplex PCR을 이용한 요로병원성 대장균의 독성 유전자의 연구를 통해 가장 우세한 요로병원 대장균의 유전자형을 알 수 있었고 독성 유전자의 보유 자체만은 요로감염의 중증도를 시사하지 않는다는 것과 독성 유전자가 없다는 것이 재발성의 가능성이 있음을 시사하지 않는다는 것을 알 수 있었다. 또 신경인성 방광과 같이 요로계 질환이 있는 경우 독성 유전자가 없는 대장균주에 의해 요로감염이 일어난다는 기존의 주장과 달리 sfa^+ 균주가 의미있게 많았으나 이에 대해서는 더 자세한 연구가 필요하다고 생각된다. Background : We identified virulence genes in uropathogenic E. coli isolates and studied the association between virulence gene and clinical characteristics in order to predict the severity and recurrency. Methods : 39 Escherichia coli strains from patients with urinary tract infection were clinically and genotypically characterized. The strains were examined genotypically by using the multiplex polymerase chain reaction for presence of 5 urovirulence genes : pyelonephritis-associated pili(pap), S. fimbriae(sfa), afimbrial adhesin(afa), cytotoxic necrotizing factor (cnf), and α-hemolysin(hly). The patient`s clinical characteristics were determined retrospectively. Results : 17 pap^+, 4 sfa^+, 7 afa^+, 6 cnf^+, and 8 hly^+ strains were identified. And there were 10 genotypes. Among them, genotype pap^+sfa^-afa^-cnfhly^- was most dominant(36%). But no urovirulence gene was detected in 12 strains(31%). When the data was analyzed, it was apparent that an association among various urovirulence genes exists. sfa gene was frequently associated with cnf gene(p<0.001). And afa gene was associated cnf and hly gene(p=0.026, <0.001). An association between cnf gene and hly gene was observed(p=0.002). Positive rates of virulence genes were not different between male and female. In infancy, pap^-sfa^-afa^+cnf^+hly^+ genotype wasdominant. In 2-15 years old age group, papsfa^-afa^-cnf^-hly^- genotype was dominant. And in 16-40 years old age group, pap^+sfa^-afa^-cnf^-hly^- was dominant. So, some virulence genotype might be associated with specific age group. Presence of virulence gene or specific genotype was not different among diseases(acute pyelonephritis, cystitis, asymptomatic bacteriuria). So, virulence genes were not associated with severity of urinary tract infection. Virulence genes were not associated with susceptibility of recurrent infection. In neurogenic bladder patients, there were significantly more sfa^+ strains (p=0.019). And all isolates of neurogenic bladder patients were genotype pap^+sfa^+afa^-cnf^+hly^-(p<0.001). Conclusion : In this study, We found which genotype is most dominant in uropathogenic Escherichia coli, and that virulence genes do not suggest severity or recurrency of urinary tract infection. In neurogenic bladder patients, some virulence genes were more prevalent.

중,소규모 사업장의 국소배기장치 설치 실태와 문제점 및 개선방안

임성근 ( Seong Keun Lim ),박두용 ( Doo Yong Park ),김원기 ( Won Ki Kim ),김수근 ( Soo Geun Kim ) 한국산업위생학회 2010 한국산업보건학회지 Vol.20 No.1

신경망과 절삭력신호 특성을 이용한 공구이상상태 감지에 관한 연구

임근영(Keun-Young Lim),문상돈(Sang-Don Mun),오성훈(Seong Hun Oh),김성일(Seong-Il Kim),김태영(Tae-Young Kim) Korean Society for Precision Engineering 1997 한국정밀공학회지 Vol.14 No.12