Re: Effect of BKCa Channel Opener LDD175 on Erectile Function in an In Vivo Diabetic Rat Model

Li, Jinhong,Yang, Xingliang,Yuan, Jiuhong Elsevier 2017 The journal of sexual medicine Vol.14 No.4

CALM1 rs3179089 polymorphism might contribute to coronary artery disease susceptibility in Chinese male: a case–control study

Huang Jingyan,Huang Siyun,Li Jinhong,Li Minhua,Gong Lin,Li Tongshun,Gu Lian 한국유전학회 2022 Genes & Genomics Vol.44 No.4

Background: Calmodulin 1 (CALM1) mutations are involved in the development of coronary artery disease (CAD). However, the relationship of CALM1 rs3179089 polymorphism with CAD is unknown. Objective: This study aimed to identify the relationship of CALM1 rs3179089 polymorphism with CAD susceptibility, CALM1 expression, blood pressure, blood glucose, blood coagulation and serum lipid levels of CAD patients. Methods: 550 CAD patients and 550 control subjects were genotyped for CALM1 using Sequenom MassARRAY technology. CALM1 expression level was measured by quantitative real time polymerase chain reaction (qRT-PCR). Results: CALM1 mRNA expression was higher in CAD patients than that in control subjects (P < 0.001). CAD patients with CC genotype had higher CALM1 mRNA expression level than control subjects with CC genotype (P = 0.006). Genotypic frequency of rs3179089 was different between male patients of CAD and control subjects (P = 0.045). Rs3179089 polymorphism was related to CAD risk of males in recessive model (P = 0.039). Moreover, rs3179089 polymorphism was associated with systolic blood pressure (SBP), diastolic blood pressure (DBP), fasting plasma glucose (FPG), and D-Dimer (D-D) level of patients with CAD in recessive model (P = 0.013 for SBP; P = 0.034 for DBP; P = 0.004 for FPG; P = 0.046 for D-D). In addition, rs3179089 polymorphism was correlated with low-density lipoprotein cholesterol (LDL-C) and total cholesterol (TC) serum levels of patients with CAD in both addictive (P = 0.025 for LDL-C; P = 0.001 for TC) and recessive models (P = 0.001 for LDL-C; P = 0.001 for TC). Conclusion: CALM1 expression is associated with development of CAD. CALM1 rs3179089 polymorphism affects CAD susceptibility in males, and blood pressure, blood glucose, blood coagulation and serum lipid of CAD patients.

Dynamical Expression of MicroRNA-127-3p in Proliferating and Differentiating C2C12 Cells

Li, Jie,Wang, Gaofu,Jiang, Jing,Zhou, Peng,Liu, Liangjia,Zhao, Jinhong,Wang, Lin,Huang, Yongfu,Ma, Youji,Ren, Hangxing Asian Australasian Association of Animal Productio 2016 Animal Bioscience Vol.29 No.12

MicroRNAs (miRNAs) are highly conserved, short non-coding RNAs that regulate gene expression at the posttranscriptional level. Although many miRNAs are identified in muscles and muscle cells, their individual roles are still not fully understood. In the present study, we investigated a muscle highly-expressed miRNA, miR-127-3p, in C2C12 myoblasts and tissues of goats with different muscle phenotypes (Boer vs Wushan black goats). Our results demonstrated that i) miR-127-3p was extensively expressed in tissues of goats; ii) miR-127-3p was higher expressed in muscle, spleen, heart, and skin in the muscular goats (Boer goats) than the control (Wushan black goats). Then we further characterized the dynamical expression of miR-127-3p, MyoD, MyoG, Myf5, Mef2c, and Myosin in the proliferating and differentiating C2C12 myoblasts at day of 0, 1, 3, 5, and 7 in culture mediums. Especially, we found that miR-127-3p was significantly higher expressed in the proliferating than differentiating cells. Our findings suggest that miR-127-3p probably plays roles in the proliferation and differentiation of myoblasts, which further underlies regulation of muscle phenotype in goats.

Crystal Structure Transformation and Dielectric Properties of Polymer Composites Incorporating Zinc Oxide Nanorods

Jinhong Yu,Wei Wu,Dan Dai,Yingze Song,Chaoyang Li,Nan Jiang 한국고분자학회 2014 Macromolecular Research Vol.22 No.1

Zinc oxide (ZnO) nanorods were synthesized using a modified wet chemical method. Poly(vinylidene fluoride-co-hexafluoropropylene), P(VDF-HFP), nanocomposites with different ZnO nanorods loadings were prepared viaa solution blend route. Field emission scanning electron microscopic (FE-SEM), X-ray diffraction (XRD), Fouriertransform infrared spectroscopy (FTIR) were used to investigate the structure and morphology of the nanocomposites. XRD and FTIR data indicate that the incorporation of ZnO nanorods promote the crystalline structure transformationof P(VDF-HFP). As the content of ZnO nanorods increases, the β phase structure increases while the α phasedecreases. In addition, the dielectric properties of the P(VDF-HFP) and its composites were systematically studied.

Effects of Ti and La Additions on the Microstructures and Mechanical Properties of B-Refined and Sr-Modified Al–11Si Alloys

Chenlin Li,Ye Pan,Tao Lu,Lijun Jing,Jinhong Pi 대한금속·재료학회 2018 METALS AND MATERIALS International Vol.24 No.5

The effects of Ti and La additions on the microstructures and mechanical properties of B-refined and Sr-modifi ed Al–11Sialloys were investigated in the present work. The interactions among Ti, La, B and Sr elements were discussed employingmicrostructure observation, thermal analysis and tensile test, respectively. It was found that the addition of 0.05 wt% Binduces a transformation of eutectic Si from fi nely fi brous to coarsely plate-like morphology in the Al–11Si alloy with 0.02wt%Sr modifi cation, owing to the poisoning of IIT mechanism, and the eutectic Si grows only with TPRE mechanism. Bothtitanium and lanthanum can neutralize the co-poisoning effect between Sr and B in the Al–11Si alloy, but the neutralizingeffect of La is dependent on the addition sequence. The combinative addition of La and B elements promotes the effectiverefi nement of α-Al grains, but an inhomogeneous modifi cation of eutectic Si phases is also observed, leading to a slightlydecrease in the elongation.

Evolution of the Transient Saturated Zone and Stability Analysis of Slopes under Rainfall Conditions

Xiang Qiu,Jinhong Li,Huangbin Jiang,Jian Ou,Jiqian Ma 대한토목학회 2022 KSCE JOURNAL OF CIVIL ENGINEERING Vol.26 No.4

To study slope stability and instability modes under rainfall conditions, an improved Bishop method is proposed according to saturated-unsaturated seepage flow theory. It can simultaneously consider the depth effect of rock and soil mass shear strength parameters, transient water pressure, groundwater pressure, saturated water softening, unsaturated strength, and pore water unit weight. The variation law of the transient saturated zone, potential slip surfaces and stability coefficient under rainfall conditions are analysed with this method. The results indicate that after rainfall starts, the transient saturated zone of the slope continuously expands, and the stability coefficient gradually decreases. After rainfall stops, the transient saturated zone of the slope dissipates rapidly, and the stability coefficient continues to increase. During the early rainfall period, the slope instability mode is deep-layer integral slippage. During the later rainfall period, the slope instability mode is shallow-layer local slippage. The depth effect and unsaturated strength are beneficial to the slope stability, while the transient water pressure, saturated water softening, and pore water unit weight are not. The application of this method in engineering practice is of great significance to slope stability analysis and protection under rainfall conditions.

Efficient Immobilization of Enzymes on Amino Functionalized MIL-125-NH2 Metal Organic Framework

Zichen Wang,Yang Liu,Jinhong Li,Guoqing Meng,Daoyu Zhu,Jiandong Cui,Shi Ru Jia 한국생물공학회 2022 Biotechnology and Bioprocess Engineering Vol.27 No.1

As a series of metal organic framework (MOFs), materials of institute lavoisier frameworks (MILs) are expected to be excellent supports for enzyme immobilization due to their good water-stability and acid tolerance. However, enzyme loading on MIL-125-NH2 is very low due to small pore size and few amino groups on the surface of the MIL-125-NH2. In this work, catalase (CAT) was immobilized on the MIL-125-NH2 and amino functionalized MIL-125-NH2 by adsorption (CAT@MIL-125-NH2) and covalent binding (CAT@Amino MIL-125-NH2), respectively. Compared with the CAT@MIL-125-NH2 and free CAT, the CAT@Amino MIL-125-NH2 displayed high activity recovery, good pH stability, stability against denaturants, and thermostability. Furthermore, activity recovery of CAT@Amino MIL-125-NH2 was 56% higher than CAT@MIL-125-NH2. The CAT@Amino MIL-125-NH2 still retained 50% residual activity for 14 days at room temperature, whereas free CAT lost activity after storage for 1 day at the same storage conditions. Furthermore, the CAT@Amino MIL-125-NH2 maintained 62% of its initial activity after 4 consecutive uses, showing good reusability. The results showed that the amino functionalized MIL- 125-NH2 is an excellent carrier of enzyme immobilization.

Sensorless Control Strategy of Permanent Magnet Synchronous Motor Based on Fuzzy Sliding Mode Controller and Fuzzy Sliding Mode Observer

Zou Xinhong,Ding Hongchang,Li Jinhong 대한전기학회 2023 Journal of Electrical Engineering & Technology Vol.18 No.3

This paper proposes a permanent magnet synchronous motor sensorless control strategy based on fuzzy sliding mode controller and fuzzy sliding mode observer. In order to improve the response speed of the system and the anti-interference ability of the system, a sliding mode controller (SMC) is used to replace the PI regulator in the speed loop. In order to suppress the chattering caused by sliding mode variable structure control and reduce the error caused by chattering, the SMC and sliding mode observer (SMO) are improved. An integral sliding mode surface is used in the SMC, and a continuous saturation function is used instead of a discontinuous sign function. The square of the speed error is introduced in the switch term of the exponential approaching law to improve the dynamic quality of approaching motion. The switching function in the SMO also uses a saturation function to suppress chattering caused by switching. Fuzzy rules are established to adaptively adjust the switching gain of the SMO and the parameters in the SMC’s reaching law, which further improves the performance of the control system. Simulations and experiments prove that the proposed control strategy has the characteristics of small chattering, fast response speed, and strong robustness.