Helicobacter pylori 편모 유전자의 클로닝 및 염기서열 분석

이광호,이우곤,조명제,도영미,백승철,강경희,박필성,이상룡 경상대학교 유전공학연구소 1993 遺傳工學硏究所報 Vol.12 No.-

A λgt11 expression libary of H. pylori DNA in E. coli Y1090 was screened with flagellin-specific rabbit antiserum for molecular cloning of the flagellin gene of H. pylori. A positive clone, λHPF4, was obtained and the recombinant antigen expressed from λHPF4 was a fusion protein with the molecular weight of 168kd. Sequence analysis of antigen-encoding DNA showed that an open reading frame composed of 1,536 nucleotides encodes a polypepride with a oredicted molecular size of 54kd. This open reading frame did not show the homology with flaA gene encoding 56kd protein of H. pylori and was confirmed as a unique sequence through homoligy searching. Therefore, the cloned antigen is supposed to be the carboxy-terminal region of the other flagellin protein of H. pylori, flaB, with the molecular weight of 58kd.

흰쥐 장에서 Cis-Diammine Dichloro Platinum(Ⅱ)의 방사선손상에 대한 효과

이경자,이정식 梨花女子大學校 醫科大學 醫科學硏究所 1995 EMJ (Ewha medical journal) Vol.18 No.4

목적 : 흰쥐의 복부에 방사선조사와 cis-DDP를 투여하여 소장과 대장의 조직학적 소견을 토대로 하여 cis-DDP가 장관에서 방사선손상에 미치는 영향을 관찰 한다. 방법 : 흰쥐의 복부에 방사선조사군(6~10 Gy), cia-DDP(2.5mg/kg)투여군, 방사선조사와 cis-DDP 병행군으로 분류하였다. 방사선조사군은 전복부에 선형가속기를 이용하여 단일조사하고 cis-DDP투여군은 복강내 1회 주입하였다. 병행군은 방사선조사 전 30분에 cis-DDP투여군과 방사선조사 직후 cis-DDP투여군으로 분류하며 cis-DDP는복강내 주입하였다 실험완료 후 30일에 동물은 개복하여소장과 대장의 조직학적 소견을 광학현미경과 전자현미경으로 비교 관찰하였다. 결과 : 소장에서 cis-DDP단독군은 점막에 경도의 염증세포침윤과 국소적인 괴사가 관찰되었다. 방사선조사 단독군에서 점막의 괴사는 8 Gy, 방사선 조사와 cia-DDP병행군의 6 Gy에서 관찰되었다. 점막하조직의 섬유화는 방사선조사 단독군은 10 Gy, 방사선조사 전 cis-DDP 투여군은8 Gy, 방사선조사 후 cis-DDP 투여군은 6 Gy에서 나타나기 시작하였으며 근층의 괴사는 3군 모두 8 Gy에서 관찰되었다 소장에서 점막하조직의 섬유화를 정량적 종말점으로 하여 증강율은 방사선조사 전 cis-DDP투여군은1.67, 방사선조사 후 cis-DDP 투여군은 1.25이었다 대장에서 점막하조직의 섬유화는 방사선조사 단독군과 방사선조사 후 cis-DDP투여군의 8 Gy에서 관찰되었으며 방사선조사 전 cis-DDP투여군의 6 Gy에서 관찰되었으며 점막하조직의 섬유화를 정량적 종말점으로 하여 증강율은방사선조사 전 cis-DDP 투여군은 1.33, 방사선조사 후투여군은 1.0이었다. 결론 : 점막하조직의 섬유화를 정량적 종말점으로 하여 증강율이 소장에서 방사선조사 전 cis-DDP투여군은 1.67, 방사선조사 후 cis-DDP투여군은 1.25이었다. 대장에서 증강율은 방사선조사 전 cis-DDP투여군은 1.33, 방사선조사후 cis-DDP투여군은 1.0이었다. Cis-DDP는 소장과 대장에서 방사선의 효과를 증강시키며 특히 cis-DDP를 방사선조사 전에 투여한 경우 방사선손상이 증가되었다. 0bjective : This experimental study was performed for evaluate the effects of cis-di-amminedichloroplatinum(Ⅱ) (cis-DDP) on the radiation injury of rat bowel by histopathologic changes. Method and materials : Rats were exposed to entire abdomen by a single doses of X-ray(6-10 Gy) without or witn cis-DDP(2.5mg/kg). Rats were divided into 3 groups such as radiationalone, cis-DDP alone and combined group. In combined group, cis-DDP was given 30minutes before or immediately after irradiation. Results : Cis-DDP induced the inflammatory cell infiltrations with focal necrosis of the mucosa in the small bowel and no abnormal change in the large bowel. In radiation alone group,mucosal necrosis, subrnucosal fibrosis and muscular necrosis were prominent changes in smallbowel and submucosal fibrosis in the large bowel. The submucosal fibrosis in the small bowelwas appealed in 10 Gy of radiation alone group and 8 Gy of cis-DDP infusion after radiationand 6 Gy of cis-DDP infusion before radiation of combined group. In the large bowel, submucosal fibrosis was noted in 8 Gy of radiation alone group and 8 Gy of cis-DDP infusion after radiation and 6 Gy of cis-DDP infusion before radiation of combined group. In the smallbowel, the enhancement ratio was 1.67 in a group of cis-DDP infusion before radiation and 125 in a group of cis-DDP infusion after radiation as the end point was the submucosal fibrosis,In the large bowel, the enhancement ratio was 1.33 in a group of cis-DDP infusion before radiation and 1.0 in a cup of cis-DDP infusion after radiation as e end point was e submucosal fibrosis. Conclusion : This study suggested that cis-DDP enhance the radiation effect in the small andlarge bowel especially when cis-DDP was infused before radiation.

總輸膽管 結紮術후 Hepatic Glycogen 變化에 관한 形態學的 硏究

李亨周,李永浩,朴景蘭,金源式 충남대학교 의과대학 지역사회의학연구소 1991 충남의대잡지 Vol.18 No.1

For the study on the changes of hepatic glycogen after common bile duct ligation, Sprague -Dawley rats (b.w. 250± 20gm, male) were used as experimental animal. In expermental group, at 3hrs, 6hrs, 9hrs, 12hrs, 24hrs, 48hrs, 72hrs, 96hrs, and 120hrs after ligation, the common bile duct at the point of about 1cm proximal portion from its duodenal opening, the liver specimens were collected under ether anesthesia. Simple laparotomy was performed in control rats. After fixation, the specimens were sectioned 5㎛ thickness and stained with Periodic Acid Schiff, and observed under light microscope. Also, transmission electron microscopic photos were obtained with H-7000(Hitachi Co.) TEM. The results were as followings: 1. PAS reactions were changed into negative 6hours after common bile duct ligation. 2. Glycogen depletion after common bile duct ligation was closely related with functional lobule, but not in simple laparotomized rats. 3. In the common bile duct ligated rats, PAS negativity continued in the zone I until 120hours after ligation.

정신분열병 환자에서 사회인구학적 특징과 종교에 따른 정신병 치료방법에 대한 선호도

이창민,이종일,신경희,이민규,김태현 大韓神經精神醫學會 2001 신경정신의학 Vol.40 No.5

연구목적 : 종교가 정신분열병 치료에 도움을 주기도 하지만 정신병 치료방법에 대한 잘못된 개념을 심어주어 환자의 치료순응도에 나쁜 영향을 미치기도 한다. 그러므로 종교에 따른 정신병 치료방법에 대한 선호도를 조사하여 종교가 정신분열병 치료에 미치는 영향을 알아보고자 이 연구를 시행하였다. 방 법 : DSM-Ⅳ에 의해 진단 받았으며 GAF score가 41이상은 정신분열병 환자 102명을 대상으로 하였다. Allport와 Ross의 내재적-외향적 종교성향척도를 이용하여 종교성향을 설문 조사하였으며 정신병 치료방법(초자연적 치료, 원시적 약물치료, 심리적 치료, 종교적 치료, 생물학적 치료)에 대한 선호도를 설문 조사하였다. 수집된 자료에서 사회인구학적 특성과 종교성향 그리고 정신병 치료방법의 관계를 비교 분석하였다. 결 과 : 1) 정신분열병 환자는 생물학적 치료와 심리적 치료에 가장 높은 선호도를 보였으며 다음으로 종교적 치료와 원시적 약물치료에 높은 선호도를 보였다. 2) 기독교를 믿는 환자군은 종교적 치료에 유의한 선호도를 보인다. 3) 내재적 성향의 환자군이 반종교적 성향의 환자군에 비해 심리적 치료에 유의하게 높은 선호도를 보였다. 그리고 친종교적 성향의 환자는 초자연적 치료, 심리적 치료 그리고 종교적 치료에서 유의하게 높은 선호도를 보였다. 결 론 : 전반적으로 종교성향과 정신병 치료방법은 유의한 상관관계를 보이지 않았다. 그러나 종교성향은 특정한 교리나 사상을 반영하기보다는 개인의 성격을 반영하는 경향이 있으므로 종교과 정신병 치료방법이 전혀 무관하다고 할 수 없다. 환자들은 종교적 치료와 원시적 약물치료에 상당한 선호도를 보이고 있으며 특히, 기독교를 믿는 환자군은 종교적 치료에 유의한 선호도를 보인다. 그러므로 환자와 가족에게 정신분열병에 대한 정신교육을 지속적으로 시행해야 한다고 여겨진다. Objectives : Religion may be beneficial to the treatment of schizophrenia, but religion frequently have bad influence on drug compliance because religion give false concept of the treatment of psychosis. We investigated the influence of religion on the treatment of schizophrenia by assessing the preference for the treatment of psychosis to religion. Methods : 102 patients of schizophrenia who were diagnosed by DSM-Ⅳ and above 41 scores of GAF score were selected. We assessed religious orientation with the Intrinsic and Extrinsic Religious Orientation Scale and assessed the preference for the treatment method of psychosis(supernatural treatment, primitive medicinal treatment, psychological treatment, religious treatment, biological treatment). The relationship among the variables of demographic data, religious orientation, the treatment method of psychosis were analyzed. Results : 1) Schizophrenic patient showed first preference for biological treatment and psychological treatment and they showed second preference for religious treatment and primitive medicinal treatment. 2) Protestant showed preference for religious treatment. 3) Patient group who have intrinsic religious orientation showed more preference for psychological treatment than patient group who have antireligious orientation. Conclusions : Generally the relation between religious orientation and the treatment method of psychosis was not significant. But it couldn't be said that religion and the treatment method of psychosis was not related because religious orientation was not related with religious ideology but was related with personality. Schizophrenic patient showed more preference for religious treatment and primitive medicinal treatment and specifically, protestant showed preference for religious treatment. Therefore, we suggest that it is necessary to give persistent education about schizophrenia to patients and their family.

정신감성 59례에 대한 정신의학적 고찰

이충순,이인표,이주호,성경희 용인정신의학연구소 1994 용인정신의학보 Vol.1 No.1

맞벌이 가정의 가족스트레스에 관한 연구

李賢鈺,金良喜,李慶姬,洪秉淑 中央大學校 家庭文化硏究所 1989 가정문화논총 Vol.3 No.-

The purpose of this study was to understand the contents of family stresses among dual earners in urban area. Selected research objectives included the coping behaviors, adoptation methods, and the effects of family resources with family stresses. For this purpose, 142 samples were selected and interviewed. It was found that there were close relationships among the family resources, coping behavior, adoptation methods and family stresses of dual earners. Different style of coping behavior and adoptation methods seemed to change the degree of family stress, and the family resources were the most important factor that influenced the family stresses of dual easners.

제조업 근로자의 작업환경인지도와 스트레스와의 관련성 연구

오영아,이명선,이경용 大韓産業醫學會 2002 대한직업환경의학회지 Vol.14 No.3

목적 : 한국산업안전공단의 '1999년 근로자건강실태조사'를 분석의 기초자료로 하였으며, 연구대상인 전국의 근로자 6,764명을 대상으로, 신체적 스트레스, 정신적 스트레스와 일반적 특성, 작업조건과의 관련성을 분석하였다. 방법 : 표본으로 추출된 근로자들에게 구조화된 설문지를 통하여 1:1 면접조사를 시행하였으며, 자료는 SPSS 10.0을 이용하여 통계 처리하였다. 결과 : 1. 일반적 특성에 따른 스트레스 수준은 여성이 남성보다 높은 수준을 보여주었으며(p<0.001), 연령이 낮을수록(p<0.001), 규모는 클수록 그 수준이 높았다(p<0.01). 2. 근로조건에 따른 스트레스 수준은 작업시간이 많을수록(p<0.001), 휴식시간이 없을 때(p<0.05) 그 수준이 증가하였고, 작업공간이 좁고 작업밀도가 강할 때 스트레스가 높았다(p<0.001). 물리적 작업 환경에서는 습도와 작업장안전에서 문제가 있다고 느낄 때 스트레스가 높았으며(p<0.01), 소음이 문제가 있고(p<0.01), 분진 및 환기와 휴식공간과 식당, 채광조명에 문제가 있을 때 스트레스 수준이 높았다(p<0.05), 사회적 작업환경에서는 직무요구도와 직무통제성이 높고, 사회적지지가 낮을 때 스트레스 수준이 증가하였고, 이는 통계학적으로 유의하였다(p<0.001). 3. 다단계 중회귀분석을 통한 신체적 스트레스에 영향을 미치는 요인을 분석한 결과 작업장 안전이 5.1%로 가장 높은 설명력을 보여주었고, 다음으로 직무요구도, 연령, 작업밀도, 습도, 성별, 작업시간, 규모, 공식적인 휴식시간 유무의 순으로 선정되었는데 이들 요인에 의한 전체 설명력은 12 %였다. 또한 정신적 스트레스에 영향을 밀치는 요인을 분석한 결과 직무 요구도가 4.8 %로 가장 높은 설명력을 보여주었고 다음으로 습도, 연령, 성별, 직무 통제성, 작업장 안전, 사회적지지, 작업공간, 규모, 채광조건, 공식적인 휴식시간 유무, 작업시간의 순으로 선정되었는데 이들 요인에 의한 전체 설명력은 13.3 %였다. 결론 : 근로자의 작업환경이 스트레스와 관련성이 높은 것을 알 수 있다. 근무조건, 물리적 작업환경, 사회적 작업환경을 꾸준히 관리하고 개선할 때, 근로자의 스트레스가 감소할 것이고, 작업능률을 극대화 할 수 있을 것이다. Objectives : In this study we performed an analysis of the stress associated with the working conditions of 6,764 workers based on 'A survey of healthu condition of workers on 1999' by the Korean Occupational Safety & health Agency. The Purpose of this study was to provide basic data for health education and management for industrial workers' health promotion. Methods : Collected data were analyzed using the t-test. ANOVA and mutiple regression analysis, using the SPSS 10.0 program. Results : When the degrees of stress were analyzed according to demographic characteristics, females were found to have higher levels of stress than males (p<0.001). Furthermore, as age was lowered and as the work apsce became larger, the degrees of a stress were also found to be higher (p<0.001 and p<0.01, respectively). When the degrees of stress were analyzed according to working conditions, longer working hours (p<0.001) and reduced break times (p<0.05), gave rise to higher degrees of stress. Also, as the workspace was reduced in size and as the work intensity was augmented, the degrees of a stress were made higher (p<0.01). In terms of physical working conditions, humidity and a lack of sense of security resulted in higher degrees of stress (p<0.01). When the wokers sense that their environment is noisy or dusty has poor lighting or ventilation or that the rest room and cafeteria facilities are inade-quate, the degrees of stress are found to be higher. As regards the social environment, when the workers sensed that the degree of job demand or job control was higher or that the degree of social support was lower, the degrees of stress were increased (p<0.001). Among those factors which influenced physical stress in the stepwise multiple regression analysis, the primary factor was found to be the safety of the working place (5.1 %). Other factors which influenced physical stress were degree of job demand, age, work intensity, humidity, sexual discrimination, length of working hours, size of work space and the existence or not of a regular break time in this order. Total R^2 due to these factors was 12%. Among those factors which influenced mental stress in the stepwise multiple regression analysis, the primary factor was the degree of job demand (4.8 %). Others factors which infuenced mental stress were humidity, age, sexual deicrimination, the degree of a job control, safety of the working place, degree of social support, a working site, size of the work space, quality of lighting, the existence or not of a regular break time and length of working hours in this order. Total R^2 due to these factors was 13.1 %. Conclusions : The above results shows that working conditions are strongly related to stress. Furthermore, when both physical and social working conditions are contimuously managed and improved, workers may not only reduce their levels of stress but also maximize their working efficiency.

경상도 별미김치의 표준화 연구

한지숙,이숙희,이경임,박건영 동아시아식생활학회 1995 동아시아식생활학회지 Vol.5 No.2

This study was conducted to standardize ingredient ratio and preparation method of major traditional special kimchies in Kyungsang province, Korea. There were about 35 varieties of special kimchi in Kyungsang province. Six varieties of them such as burdock kimchi, wild leek kimchi, green thread onion kimchi, perilla leaf kimchi, Godulbaegi(Korean wild lettuce) kimchi, and red pepper leaf kimchi were selected, because they tasted good and the physiological functions of their main ingredients were excellent. The ingredient ratios of the selected special kimchi were standardized through surveying hereditary preparation of some families in Kyungsang province and using the literatures including cooking books. The standardized ingredient ratio of the burdock kimchi was 15.1 pickled anchovy juice, 6.8 red pepper powder, 5.7 garlic, 2.2 ginger, 18.0 rice flour paste, 13.5 green thread onion, and 1.2 sesame seed in proportion to 100 of burdock. The standardized preparation step of the selected special kimchies was similar except some preprocessing methods of main ingredients. The diagonally cut-up burdock was usually parboiled or soaked in salted water, then it was mixed with the other ingredients. Wild leek and green thread onion were usually pickled with salt or pickled anchovy juice. Sometimes the green thread onion pickled was dried in the sun. General preprocessing of perilla leaf, Korean wild lettuce, and red pepper leaf was soaking them in salted water for about 5-10 days. Sometimes red pepper leaf was heated with steam and dried in the sun, then it was mixed with the other ingredients.

Helicobacter pylori와 대장균의 Shuttle Vector 개발

조명제,이우곤,이상룡,김경희,안영숙,김성희,김현주,류복덕,최여정,윤영혜,백승철,전영석,이광호 경상대학교 유전공학연구소 1997 遺傳工學硏究所報 Vol.16 No.-

In this study, a vehicle vector using cryptic plasmids was constructed for gene transfer in Helicobacter pylori. pHP51(3.9 kb) and pHP489(1.2 kb) were selected for constructing vectors from cryptic plasmid of H. pylori isolates in Korea. The HindⅢ-digestedDNA fragment(1.2kb) of pHP489 and 1.6kb DNA fragment of pHP51 were ligated with a kanamycin resistance gene(aph3'-Ⅲ) from C. jejuni to produce the recombinant plasmids pHP489K and pHP51K, respectively. Transformation frequency of pHP51K by electroporation was low. But pHP489K could be effectively transformed into various H. pylori strains. In order to design an intermdiate vehicle vector for gene transfer into H. pylori, pBlueHP489K was prepared by recloning pHP489K DNA into pBluescript and pTZ19R vector. This vector permitted the DNA fragment containing pHP489 sequence, aph3'-Ⅲ, and cloned DNA to be cut and self-ligated in the SacⅠ site after cloning. ureA and ureB gene were inserted into pBlueHP489K, resulting in pBlueHP489K/AB. The DNA fragment containing pHP489, kanamycin resistance gene(aph3'-Ⅲ), and urease structural gene was cut away from pBlueHP489K/AB and self-ligated to generate pBlueHP489K/AB. pBlueHP489K/AB made urease-negative H. pylori strains restore their urease activity. By this experiment, pBlueHP489K was confirmed to be the vehicle system for transferring H. pylori genes.

유기용제 중독에 의한 중추신경장애 1예

강성규,이경용,정호근,이영진 大韓産業醫學會 1992 대한직업환경의학회지 Vol.4 No.1

We report a case of demyelinating lesion in central nervous system in a 38-year-old female patient who suffered from muscle weakness and disability of standing by herself. She worked for 10 hours in a day at the small footwear factory about 1 year in 1990. Physical examinations showed normal except increased DTRs in the elbows and knees. Clinical laboratory findings were within normal limits. Brain CT and EMC findings were normal. But, brain MRI showed small multiple high signal intensity area on T2 weighted image in right frontal white matter, right fronto-parietal periventricular white matter with localized atrophy in genu and rostrum of corpus callosum. There were no ventilation system in the factory. By the evaluation of work environment 1½ months later, high concentration of toluene, MEK, n-hexane, MIBK, acetone and cyclohexane were detected. We suppose her demyelinating lesion was caused by toluene alone or mixed solvents with toluene and others.