중증 재생불량성 빈혈 환자의 동종골수이식 후에 병발한 간정맥폐색질환(Hepatic Veno-Occlusive Disease)의 임상 경과 및 혈액응고 인자들의 변화

이제환,이규형,최성준,민영주,김신,이정신,김상희,박찬정,지현숙,김우건 대한조혈모세포이식학회 1999 대한조혈모세포이식학회지 Vol.4 No.2

연구배경: 재생불량성 빈혈 경우에는 조혈모세포 이식 후 VOD의 발생에 관한 보고가 거의 없는 실정이다. 저자 등은 cyclophosphamide와 antithymocyte globulin으로 전처치 후에 동종골수이식을 시행한 재생불량성 빈혈 환자들을 대상으로 하여 VOD의 발생과 임상 양상, 혈액응고 인자들의 변화에 관하여 분석하여 보았다. 방법: 재생불량성 빈혈로 동종골수이식을 시행 받은 13예 환자를 대상으로 하였다. 임상적 기준에 따라 VOD를 진단하였으며 임상 경과에 따라서 경중을 분류하였다. Protein C, protein S, antithrombin Ⅲ, tPA, PAI-1 등의 혈액응고 인자들을 전처치 투여전, 골수 주입당일, 이식 후 제7, 14, 21일에 측정하였다. 결과: 7예(53.8%)에서 VOD가 발생하였으며, 경증 5예, 중등도 2예였다. VOD군에서 혈소판치가 20.000/㎕ 이상을 유지하는 데까지 걸리는 시간이 더 길었다(p=0.026). 또한 VOD군에서 적혈구 및 혈소판의 수혈 요구량이 각각 유의하게 많았다(p=0.025, 0.015). 혈액응고 인자들의 변화를 보면, 제 0일에 VOD군에서 PAI-1의 혈중 농도가 유의하게 더 높았다.(p=0.034). 결론: 재생불량성 빈혈환자에서도 조혈모세포 이식 후에 VOD의 높은 발생률을 관찰하였으며, 대부분이 경증이었다. VOD 환자에서의 혈액응고 인자들의 변화는 PAL-1이 VOD의 감별 진단을 위하여 이용될 수 있음을 시사하고 있다. Background: There are few reports about the occurrence of hepatic veno-occlusive disease(VOD) after bone marrow transplantation (BMT) for severe aplastic anemia (SAA). We analyzed the incidence and clinical outcomes of VOD and changes of coagulation factors in patients with SAA who were conditioned with cyclophosphamide plus ATG and underwent allogeneic BMT. Methods: Thirteen patients with SAA who underwent allogeneic BMT were included. A diagnosis of VOD was made according to clinical criteria, and severity of VOD was classified according to clinical course. Plasma levels of protein C, protein S, antithrombin Ⅲ, t-PA, and PAI-1 were determined before preparative chemotherapy, in the day of marrow infusion, and days 7, 14, and 21. Results: VOD occurred in 7/13(53.8%). Five were mild VOD and two were moderate VOD. Time to platelet ≥20,000/L was slower (p=0.026) and red cell and platelet transfusion requirements were higher (p=0.025,0.015) in patients with VOD . The mean plasma level of PAI-1 at 0 were higher in patients with VOD (p=0.034). Conclusion: We observed the high incidence of VOD after allogeneic BMT in patients with SAA. A majority of cases of VOD were mild. The changes of PAI-1 after BMT may be useful marker of hepatic VOD

(128, 122) Extended Reed-Solomon Codec 구현

김제환,이찬호 崇實大學校 1999 論文集 Vol.29 No.1

A (128, 122) extended Reed-Solomon cosec is designed based on 'Digital Video Transmission for Cable Television' of SCTE. The decoder is designed using a transform decoding method, and Berlekamp-Massey algorithm is used to compute the error-locator polynomial. The decoder is composed of syndrome calculation unit, a frequency domain error vector calculation unit, and a inverse Fourier transform unit. The codec is implemented in a CPLD chip and the encoded and decoded words are measured and verified to be correct.

실리카흄을 흔입한 고강도 콘크리트 개발

홍창우,김태경,김경진,김성환,김남윤,심도식,이정호,윤청호,백민경,원치문,박제선,이주형,정경일 강원대학교 석재복합신소재제품연구센터 1996 석재연 논문집 Vol.1 No.-

콘크리트 구조물이 대형화됨에 따라 설계단면이 증대되어 상대적으로 많은 경제적 손실을 부담하게 된다. 따라서 단면을 감소시키면서도 소요의 하중에 안전한 구조물을 건설하기 위해서는 우선적으로 구조물 건설에 기초가 되는 고품질, 고내구성의 고강도 콘크리트 개발이 절실히 요구된다 본 연구에서는 일정 시멘트비 및 혼화재 비율하에서 소요의 워커빌리티가 확보되는 고강도 콘크리트를 시간과 온도의 변화에 따라 증기 양생하여, 압축, 인장, 휨강도 뿐만 아니라 파괴특성을 실험적으로 연구하였다. 시료 제작시 시멘트 입자 사이의 공극 및 불연속 영역을 충전하여 고밀도화하기 위한 콘크리트용 혼화재로 시멘트 비표면적이 상당히 작은 초미립 분말인 실리카흄을 이용하였다. 또한 AE감수제 및 고성능 유동화제를 사용하여 혼화재의 첨가에 의하여 발생될 수 있는 워커빌리티의 감소를 방지하였다. 실험결과에 의하면 일정 양생 지속 시간하에서 온도의 증가에 따라 콘크리트의 압축, 인장 및 휨강도가 전반적으로 증가하였다. 동일하게 일정 온도하에서 양생 지속시간이 커짐에 따라 강도들이 증가됨을 알 수 있었다. 따라서 상대적으로 높은 온도와 긴 지속 양생 조건하에서 파괴에 대한 저항력이 크게 나타났다. The compressible, tensible, and flexibleresistance of the high strength concrete is analyzed by the experimentation in the present study. For the test, we cure several samples with the silica fume as a mixture being become dense the spaces between the particle of cement under the variation of both the temperature and the curing-interval. Then, the superplasticizer and the ezcon are also used to satisfy the required workability for construction. The compressible, tensible, and flexible resistances to a stress are increased as increasing the temperature and the time interval for the curing. Therefore it is concluded that the overal fractural and mechanical properties is improved by mixing the silica fume into the cement.

악성 혈액질환에서 성공적인 동종골수이식 후 숙주 기질 미세환경의 구축

조상희,이제중,남찬은,최경상,정익주,이일권,김진희,박종태,김형준 대한조혈모세포이식학회 2002 대한조혈모세포이식학회지 Vol.7 No.1

인체의 골수는 간엽모세포를 함유하고 있으며 이들은 골수미세환경의 주된 세포들로 분화가 가능하여 조혈기능을 지지한다. 본 연구에서는 성별이 다른 동종조혈모세포이식 환경에서 조혈모세포의 완전 생착을 보이고, 이식 후 1년에서 8년이 지난 11예의 재생불량성 빈혈 및 백혈병 환자들을 대상으로 하여 골수에서 MSC를 분리하고 체외 확장을 통해 배양된 MSC에서 X 염색체 탐식자를 이용한 FISH 및 microsatellite polymorphism PCR 기법으로 그 기원을 확인하였다. 그 결과 조혈모세포는 완전히 공여자 기원으로 대치되었음에도 불구하고 MSC는 모두 수여자 기원임을 알 수 있어, 동종조혈모세포이식에서 미세환경의 구축은 수여자의 자가 생산에 의한 골수 간질세포에 의한 것으로 생각된다. Background: Human bone marrow (BM) contains mesenchymal stem cells (MSC) that can differentiate into various cells of mesenchymal origin. It remains a matter of controversy whether donor-derived stromal cells are capable of engraftment following hematopoietic stem cell transplantation (HSCT) or not. Methods: To determine if donor-derived stromal cells are transferred to the recipients of allogeneic HSCT, we investigated the characterization of MSC in 11 patients 1 to 8 years after sex mis-matched allogeneic HSCT in severe aplastic anemia and leukemia. Results: All patients had complete engraftment with donor- derived stem cells as shown by detection of donor type DNA in peripheral blood mononuclear cells. Following culture, MSC showed the expression of SH2 and SH4, but none of the hematopoietic markers of CD14, CD34, or CD45. MSC which can be differentiated to osteogenic lineage showed the genotype of recipient completely using FISH or PCR analysis. Conclusion: This study confirmed that MSC isolated from recipients of allogeneic HSCT in severe aplastic anemia and leukemia are not of donor genotype despite of full hematopoietic engraftment with donor type. Donor cells did not contribute to reconstitute the marrow microenvironment.

북한 벼 품종의 지대별 출수반응

양원하,김덕수,전용희,조영찬,김제규,이문희 한국국제농업개발학회 2004 韓國國際農業開發學會誌 Vol.16 No.1

북한벼의 출수 반응을 검토하기 위하여, 작물시험장 인공기상실과 표고가 다른 수원, 철원, 진부 등 3 지역에서 이앙기를 달리하여 1998년과 1999년에 시험 수행한 결과를 요약하면 다음과 같다. 가. 지대별 이앙기 변동에 다른 출수일수 변화는 표고가 낮은 지역일수록, 이앙기가 늦어질수록 출수일수가 짧아졌으며, 지역과 이앙기 이동에 따른 경향을 살펴보면, 표고가 높은 진부지역에서 이앙기가 늦어질수록 출수일수가 단축되는 정도가 뚜렷하게 나타났다. 2. 북한 벼의 기본영양생장성은 조생종은 대조 품종인 오대벼보다 짧은 편이었고, 중생종은 오대벼에 비하여 긴 편이었다. 다. 감온성은 대비품종인 오대벼보다 대체로 예민한 것으로 나타났으며 극조생종인 올벼 2호는 감온성이 매우 예민한 반응을 보여 ′98년에는 42일, ′99년에는 39일이었다. The experiment was carried out to determine heading response of North Korean rice cultivars at NCES(national crop experiment station). RDA. South Korea in 1998 and 1999. This experiment was tested at three different locations which were Suwon, Cheolwon and Jinbu with 5 transplanting times with 10 cultivars. Duration of heading stage of the rice plant was shortened at lower altitude and in later transplanting date, respectively. Bases on the heading days, it was classified as the basic vegetative phase(BVP) and thermo-sensitive phase(TSP) in tested cultivars. BVPs and TSPs among treated varieties were grouped with four groups. BVPs of North Korean rice cultivars for early maturity were shoter than that of Odaebyeo of Korean rice cultivars, however, those for medium maturity were longer than that of the control cultivar, Odaebyeo. Meanwhile, TSPs of North Korean rice cultivars were more sensitive than that of the check cultivar of Odaebyeo, Correlation between 1998 and 1999 was more significant in basic vegetative phase than in thermo-sensitive stage. Therefore, the heading time of the rice plant primarily depended on the period of basic vegetative phase growth.

Effects of Bacillus sp. PM8313 and β-glucan on the growth, immunity, immune-related gene transcription, intestinal microbial community, and edwardsiellosis resistance in red sea bream (Pagrus major)

Su-Jeong Lee,Won Je Jang,Mi-Hyeon Jeon,Da-In Noh,Young-Sun Lee,Ga-Hyun Park,Sang Woo Hur,Seunghan Lee,Bong-Joo Lee,Kang Woong Kim,Chan-Hee Kim,Jong Min Lee,Eun-Woo Lee 한국수산과학회 양식분과 2022 한국수산과학회 양식분과 학술대회 Vol.2022 No.5

CHARACTERIZATION OF A NOVEL DIVERGENT CALMODULIN ISOFORM FROM SOYBEAN

Lee, Sang-Hyoung,Kim, Jong-Cheol,Lee, Mal-Soon,Cheong, Yong-Hwa,Yoon, Hae-Won,Lim, Chae-Oh,Hong, Jong-Chan,Bahk, Jeong-Dong,Lee, Sang-Yeol,Hwang, In-Hwan,Cho, Moo-Je Plant Molecular Biology & Biotechnology Research C 1993 Plant molecular biology and biotechnology research Vol.1993 No.

Discovery of an integrative network of microRNAs and transcriptomics changes for acute kidney injury

Lee, Chan Gyu,Kim, Jin Geol,Kim, Hyun Joo,Kwon, Hyuk-Kwon,Cho, Il Je,Choi, Dal Woong,Lee, Woo Hyung,Kim, Won Dong,Hwang, Se Jin,Choi, Sangdun,Kim, Sang Geon International Society of Nephrology 2014 Kidney international Vol.86 No.5

The contribution of miRNA to the pathogenesis of acute kidney injury (AKI) is not well understood. Here we evaluated an integrative network of miRNAs and mRNA data to discover a possible master regulator of AKI. Microarray analyses of the kidneys of mice treated with cisplatin were used to extract putative miRNAs that cause renal injury. Of them, miR-122 was mostly downregulated by cisplatin, whereas miR-34a was upregulated. A network integrating dysregulated miRNAs and altered mRNA expression along with target prediction enabled us to identify Foxo3 as a core protein to activate p53. The miR-122 inhibited Foxo3 translation as assessed using an miR mimic, an inhibitor, and a Foxo3 3′-UTR reporter. In a mouse model, Foxo3 levels paralleled the degree of tubular injury. The role of decreased miR-122 in inducing Foxo3 during AKI was strengthened by the ability of the miR-122 mimic or inhibitor to replicate results. Increase in miR-34a also promoted the acetylation of Foxo3 by repressing Sirt1. Consistently, cisplatin facilitated the binding of Foxo3 and p53 for activation, which depended not only on decreased miR-122 but also on increased miR-34a. Other nephrotoxicants had similar effects. Among targets of p53, Phlda3 was robustly induced by cisplatin, causing tubular injury. Consistently, treatment with miR mimics and/or inhibitors, or with Foxo3 and Phlda3 siRNAs, modulated apoptosis. Thus, our results uncovered an miR integrative network regulating toxicant-induced AKI and identified Foxo3 as a bridge molecule to the p53 pathway.

Magnetically-separable and highly-stable enzyme system based on crosslinked enzyme aggregates shipped in magnetite-coated mesoporous silica

Lee, Jinwoo,Na, Hyon Bin,Kim, Byoung Chan,Lee, Jin Hyung,Lee, Byoungsoo,Kwak, Ja Hun,Hwang, Yosun,Park, Je-Geun,Gu, Man Bock,Kim, Jaeyun,Joo, Jin,Shin, Chae-Ho,Grate, Jay W.,Hyeon, Taeghwan,Kim, Jungb Royal Society of Chemistry 2009 Journal of materials chemistry Vol.19 No.42

<P>A magnetically-separable and highly-stable enzyme system was developed by adsorption of enzymes in superparamagnetic hierarchically ordered mesocellular mesoporous silica (M-HMMS) and subsequent enzyme crosslinking. Superparamagnetic nanoparticles were homogeneously incorporated into hierarchically-ordered mesocellular mesoporous silica (HMMS) by the decomposition of a preformed iron propionate complex. The size of the incorporated superparamagnetic nanoparticles was around 5 nm, generating a magnetically separable host with high pore volumes and large pores (M-HMMS). α-chymotrypsin (CT) was adsorbed into M-HMMS with high loading (∼30 wt%) in less than 30 minutes. Glutaraldehyde (GA) treatment of adsorbed CT resulted in nanometer scale crosslinked enzyme aggregates in M-HMMS (CLEA-M). The activity of these CT aggregates in M-HMMS (CLEA-M-CT) was 34 times than that of simply adsorbed CT in M-HMMS, due to an effective prevention of enzyme leaching during washing <I>via</I> a ship-in-a-bottle approach. CLEA-M-CT maintained the initial activity not only under shaking (250 rpm) for 30 days, but also under recycled uses of 35 times. The same approach was employed for the synthesis of CLEA-M of lipase (CLEA-M-LP), and proven to be effective in improving the loading, activity, and stability of enzyme when compared to those of adsorbed LP in M-HMMS.</P> <P>Graphic Abstract</P><P>Crosslinked enzyme aggregates in magnetitie-coated mesoporous silica, fabricated <I>via</I> a two-step procedure of enzyme adsorption and crosslinking, were highly active, highly stable, and easily recyclable using a magnet. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=b909109b'> </P>

Anticancer effects of an extract from the scallop <i>Patinopecten yessoensis</i> on MCF-7 human breast carcinoma cells

Lee, Chu,Chun, Wonjoo,Zhao, Rongjie,Kim, Young Dae,Nam, Myung Mo,Jung, Dae Hwa,Cho, Il Je,Jegal, Kyung Hwan,Lee, Tae Hoon,Kim, Young Woo,Park, Sang Mi,Ju, Seong A.,Lee, Chul Won,Kim, Sang Chan,An, Won D.A. Spandidos 2017 Oncology letters Vol.14 No.2

<P><I>Patinopecten yessoensis</I>, is a species of scallop and a marine bivalve mollusk. In traditional East Asian medicine, scallop meat is used as a drug for the treatment of diabetes, pollakisuria, and indigestion. The present study was conducted in order to examine the potential anticancer effects of scallop flesh extract (SE) on MCF-7 human breast cancer cells. An MTT assay was used to evaluate cell viability and flow cytometry was used for the assessment of cell cycle distribution and apoptosis. The alteration in protein expression level was determined by western blot analysis, and the amounts of docosahexaenoic acid and eicosapentaenoic acid in the SE were measured by gas chromatography. SE inhibited the growth of MCF-7 human breast cancer cells in a dose-dependent manner by inducing G0/G1 phase arrest. The cell cycle arrest was associated with the upregulation of p53 and p21, and downregulation of G1 phase-associated cyclin D1/cyclin-dependent kinase (Cdk) 4 and cyclin E1/Cdk 2. In addition, SE-mediated cell cycle arrest was associated with the promotion of apoptosis, as indicated by the expression of apoptosis-associated proteins and changes in nuclear morphology. SE appeared to induce the mitochondrial apoptotic cascade, as indicated by a decreased expression of Bcl-2, activation of Bcl-2 associated X protein, release of cytochrome c, decrease in procaspase-3, and an increase in cleaved-poly (ADP-ribose) polymerase (PARP). Furthermore, the expression levels of Fas-associated via death domain and cleaved caspase-8 were increased in a SE dose-dependent manner. Taken together, these results suggest that the intrinsic and extrinsic pathways of apoptosis are associated with the anticancer effects of SE on MCF-7 cells. Thus, SE may be a suitable candidate for the treatment and prevention of human breast cancer.</P>