ST6Gal-I Predicts Postoperative Clinical Outcome for Patients with Localized Clear-cell Renal Cell Carcinoma

Liu, Hai-Ou,Wu, Qian,Liu, Wei-Si,Liu, Yi-Dong,Fu, Qiang,Zhang, Wei-Juan,Xu, Le,Xu, Jie-Jie Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.23

Hyperactivated ${\alpha}2$-6-sialylation on N-glycans due to overexpression of the Golgi enzyme ${\beta}$-galactoside: ${\alpha}2$-6-sialyltransferase (ST6Gal-I) often correlates with cancer progression, metastasis, and poor prognosis. This study was aimed to determine the association between ST6Gal-I expression and the risk of recurrence and survival of patients with localized clear-cell renal cell carcinoma (ccRCC) following surgery. We retrospectively enrolled 391 patients (265 in training cohort and 126 in validation cohort) with localized ccRCC underwent nephrectomy at a single center. Tissue microarrays were constructed for immunostaining of ST6Gal-I. Prognostic value and clinical outcomes were evaluated. High ST6Gal-I expression was associated with Fuhrman grade (p<0.001 and p=0.016, respectively) and the University of California Los-Angeles Integrated Staging System (UISS) score (p=0.004 and p=0.017, respectively) in both cohorts. Patients with high ST6Gal-I expression had significantly worse overall survival (OS) (p<0.001 and p<0.001, respectively) and recurrence free survival (RFS) (p<0.001 and p=0.002, respectively) than those with low expression in both cohorts. On multivariate analysis, ST6Gal-I expression remained associated with OS and RFS even after adjusting for the UISS score. Stratified analysis suggested that the association is more pronounced among patients with low and intermediate-risk disease defined by the UISS score. High ST6Gal-I expression is a potential independent adverse predictor of survival and recurrence in ccRCC patients, and the prognostic value is most prominent in those with low and intermediate-risk disease defined by the UISS score.

Command Filter-based Backstepping Control for the Speed and Tension System of the Reversible Cold Strip Rolling Mill Using Disturbance Observers

Le Liu,Nuan Shao,Suyan Ding,Yi-ming Fang 제어·로봇·시스템학회 2020 International Journal of Control, Automation, and Vol.18 No.5

This paper investigates the decoupling and coordinated tracking control problem for the speed and tension system of the reversible cold strip rolling mill. Using a diagonal matrix decoupling network and neural network disturbance observers, we propose a command filter-based backstepping control strategy. First, the diagonal matrix decoupling network is constructed to weaken the coupling between the speed and tension of the rolling mill system, which reduces the complexity of the system model effectively. Second, controllers are designed by combining the backstepping with the command filter, which solves the “explosion of complexity” problem in backstepping procedure, and optimizes the system’s control structure. Next, neural network disturbance observers are developed to observe the uncertain items of the system, which improve the tracking control precision of the system effectively. Theoretical analysis shows that all signals in the closed-loop system are uniformly ultimately bounded. Finally, simulation research is carried out on the speed and tension system of a 1422 mm reversible cold strip rolling mill; results show that using the proposed control strategy increased the dynamic response speed of the system by approximately 1s, and the stability precision improved by approximately 2000 N.

FTESO-based Nonsingular Fast Integral Terminal Sliding Mode Finite Time Control for the Speed and Tension System of the Cold Strip Rolling Mill

Le Liu,Ranyang Deng,Zhuang Ma,Yiming Fang 제어·로봇·시스템학회 2023 International Journal of Control, Automation, and Vol.21 No.1

To weaken the influences of uncertainties on the finite time tracking control performance of the speed and tension system of the reversible cold strip rolling mill driven by alternating current (AC) asynchronous motors, a dynamic surface backstepping nonsingular fast integrated terminal sliding mode control (DSBNFITSMC) strategy is proposed based on finite time extended state observers (FTESOs). Firstly, the FTESOs are constructed to dynamically observe the system’s mismatched uncertainties, and the observation errors can converge to zero in finite time. Next, the dynamic surface backstepping control is combined with the nonsingular fast integrated terminal sliding mode finite time control to complete the controller designs for the speed and tension system of the reversible cold strip rolling mill, which solve the “differential explosion” problem in conventional backstepping control, and simplify the design processes of the system controllers. Again, theoretical analysis shows that the proposed control strategy can guarantee the closed-loop system is practical finite time stability in the Lyapunov sense. Finally, the simulation research is carried out on the speed and tension system of a reversible cold strip rolling mill by using the actual data, and results show the validity of the proposed control strategy.

Finite-time Prescribed Performance Fault-tolerant Control for a Class of Nonlinear System Using Super-twisting Disturbance Observer

Le Liu,Xin Wang,Yueheng Sun,Yiming Fang 제어·로봇·시스템학회 2023 International Journal of Control, Automation, and Vol.21 No.12

To improve the performance of a class of nonlinear system with actuator fault and external disturbance, a fault-tolerant control method with finite-time prescribed performance is proposed based on the super-twisting disturbance observer in this paper. First, the actuator bias fault and external disturbance of the system are regarded as a lumped disturbance, and it is estimated by the designed super-twisting disturbance observer in finite time. Second, a finite-time prescribed performance fault-tolerant controller under full state constraints is designed by combining the backstepping method, the modified barrier Lyapunov function (MBLF) method and the improved prescribed performance function (IPPF) method. Moreover, the “explosion of complexity” problem in the controller design is solved by the command filters with error compensation. Theoretical analysis shows that the closed-loop system can converge to the neighborhood near the equilibrium point in finite time, and all states are always within the constraint boundaries. Finally, the effectiveness and feasibility of the proposed method are verified by the comparisons of simulations and experiments based on a permanent magnet synchronous motor (PMSM) position system.

Ganoderic acid A attenuates high‑fat‑diet‑induced liver injury in rats by regulating the lipid oxidation and liver inflammation

Fuli Liu,Kejian Shi,Jiaojiao Dong,Zhousheng Jin,Yiquan Wu,Yaoyao Cai,Tingting Lin,Qianqian Cai,Le Liu,Yujian Zhang 대한약학회 2020 Archives of Pharmacal Research Vol.43 No.7

Ganoderic Acid A (GA) has many pharmacologicaleffects such as anti-tumor, antibacterial, anti-inflammatory,and immunosuppressive effects. However, the protectiveeffect of GA on liver injury has not been reported. Thisstudy aimed to investigate the action of GA on insufficientmethionine and choline combined with high-fat diet (HFD)-induced non-alcoholic fatty liver disease (NAFLD) in rats. NAFLD model was established by insufficient methionineand choline combined with high fat feeding to rats. The levelsof Acetyl-CoA carboxylase, fatty acid synthase, sterolregulatory element binding protein, liver X receptors, AMPactivatedprotein kinase, peroxisome proliferator-activatedreceptor α, PPARg coactivator 1α and NF-κB pathway inthe liver were detected by western blot. The results of thisstudy demonstrated that the expression of GA can not onlysignificantly decrease the live weight and liver weight perbody weight of HFD mice, but also restore the alanine aminotransferase,aspartate aminotransferase, total bilirubinlevels, triglyceride and cholesterol in serum. In addition,the expression of GA increased the levels of high-densitylipoprotein cholesterol in serum, ameliorated pathologicalchanges and decreased NAS score of mice’s liver. In conclusion,the treatment with GA could improve NAFLD in ratsby regulating the levels of signaling events involved in freefatty acid production, lipid oxidation and liver inflammation.

CEO 위기책임성과 SNS 상호작용성이 위기 대응 전략 인식에 미치는 영향

유로(Le Liu),최유진(Youjin Choi) 한국콘텐츠학회 2020 한국콘텐츠학회논문지 Vol.20 No.3

선행 위기관리 연구의 초점은 주로 기업의 위기책임성에 맞추어져 있었으나 CEO에 관한 연구는 부족하다. 본 연구에서는 CEO 위기 발생시, 위기책임성과 위기 대응 전략이 CEO 태도, 기업 태도, 제품 구매의도에 미치는 영향을 규명하였다. 또 CEO의 평소 SNS 상호작용이 위기책임감과 위기 대응 전략의 영향을 어떻게 조절하는지 탐색하였다. 서울에 거주하는 중국인 학생 200명을 대상으로 2(위기책임성: 고 vs. 저) X 2(위기 대응 전략: 방어 vs. 사과) X 2(SNS 상호작용성: 고 vs. 저) 설계 실험을 실시하였다. CEO 위기책임성 수준에 따른 대응 전략의 효과를 분석한 결과, 위기책임성과 상관없이 사과 전략이 방어 전략보다 더 호의적인 CEO 태도, 기업 태도, 제품 구매의도를 나타냈다. 평상시 CEO의 SNS 상호작용이 미치는 조절 역할을 검증한 결과 CEO의 책임성이 높은 위기 경우에는 평상시 SNS 상호작용성이 높으면서 사과 전략을 구사할 때 가장 호의적인 반응이 나타났다. 그러나 CEO의 책임성이 낮은 위기 경우에는 SNS 상호작용성에 상관없이 사과 전략이 방어 전략보다 더 호의적인 반응을 이끌어 냈다. Previous crisis management research has mainly focused on business organizations’ responsibility for crises, and yet research on CEOs in crisis still lacks. This research examined the influences of crisis responsibility and response strategies on attitudes toward a CEO, attitudes toward a corporate, and purchase intentions in the case of CEOs in crisis. In addition, this study explored how a CEO’s SNS interactivity during normal times moderates the influences of crisis responsibility and response strategies. A 2(responsibility: high vs. low)X2(strategy: defensive vs. apology)X2(interactivity: high vs. low) experimental design was conducted with 200 Chinese students in Seoul. According to the analysis results from effects of response strategies by CEO’s crisis responsibility levels, regardless of the crisis responsibility levels, the apology strategy was more effective than the defensive strategy in terms of CEO attitudes, corporate attitudes, and purchasing intentions. The analysis results about the moderating role of SNS interactivity during normal times show that under the CEO’s high responsibility condition, keeping high SNS interactivity during normal times and using the apology after crisis brought out most favorable responses. Under the CEO’s low responsibility condition, regardless of the SNS interactivity levels, the apology strategy brought out more favorable responses than the defensive strategy.

Preparation of 2D/2D g-C3N4/Ti3C2 MXene composites by calcination synthesis method for visible light photocatalytic degradation of tetracycline

Qiao Le-Le,Zhang Feng-Jun,Kai Chun-Mei,Liu Chao,Wang Ying-Rui,Oh Won-Chun 한국세라믹학회 2023 한국세라믹학회지 Vol.60 No.5

In this work, an ultrathin 2D/2D g-C3N4/Ti3C2 heterojunction was synthesized by direct calcination of a mixture of urea and multilayer Ti3C2 for photocatalytic degradation of tetracycline. Among them, urea is the precursor for the generation of g-C3N4 and generates gas to peel the multilayer Ti3C2 into fewer layers while reacting, solving the problem of low yield for the preparation of fewer layers of Ti3C2. The experimental results of tetracycline degradation under visible light showed that pure g-C3N4 (UCN) exhibited weak photoactivity; however, its photocatalytic performance was enhanced when Ti3C2 was coupled with g-C3N4. The best sample (5TC) could degrade 90.1% of tetracycline within 30 min. After four cycles of stability test, the photocatalytic performance did not change significantly, indicating that the prepared 2D/2D g-C3N4/Ti3C2 heterojunction possesses strong photocatalytic performance along with good stability.

Protective effect of centaureidin-3,5-O-biglucoside (C3G) on 5-fluorouracil(5-FU)-induced mucositis in BALB/c mice

Junyang Liu,Zixian Wang,Wanying Li,Zhewen Dong,Chaojie Chong,Yaqi Wu,Shumei Zhong,Haokun Chen,Zhiqiang Liu,Qi Qi Pang,Jia-Le Song 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10

To explore the effect of C3G on intestinal mucosal injury in 5-FU induced BALB/c mice. Mice were randomly divided into normal, model and C3G groups. After the last administration, mice were anesthetized, and calculated the thymus and spleen indexes. The ileum was used to stain with H&E observation. Serum levels of IL-1β, IL-6, IL-10 and TNF-α were determined by ELISA kits. Compared with the normal group, the model group had diarrhea, body weight and thymus and spleen index decreased significantly, intestinal villus epithelial cells fell off, crypt structure was destroyed, and inflammatory cell infiltration was observed. The body weight, thymus and spleen indexes of the treatment group and the prevention group decreased less, and the levels of TNF-α, IL-1β and IL-6 were significantly decreased, and the level of IL-10 was significantly increased relative to the model group. The intestinal villi structure was relatively complete, and a small amount of crypt structure was destroyed, with a small amount of inflammatory cell infiltration, which reduced the mucosal damage. The administration of C3G could protect intestinal mucosa from 5-FU-induced injury.

Effect of Antrodia camphorata polysaccharides on letrozole-induced polycystic ovary syndrome (PCOS) model rats

Zhiqiang Liu,Chaojie Chong,Zhewen Dong,Yaqi Wu,Wanying Li,Haokun Chen,Shumei Zhong,Junyang Liu,Qi Qi Pang,Jia-Le Song,Yanyuan Zhou 한국식품영양과학회 2021 한국식품영양과학회 학술대회발표집 Vol.2021 No.10

To investigate the effect of ACP on letrozole-induced PCOS rats. All rats were divided into normal groups, PCOS group, metformin group and ACP group. The body weight (BW) was recorded every three days, and the final body weight as well as ovarian weight were recorded. Serum levels of TC, TG, HDL-C, LDL-C and E2, T, FSH and LH were measured by ELISA assay, and the LH/FSH ratio was calculated. The results showed that ACP decreased the BW and the ratio of ovarian to BW. Compared with the normal group, the serum levels of T, LH, LH/FSH, TG, TC, LDL-C increased, and the levels of FSH, E2, HDL-C decreased in PCOS group. ACP effectively improves the serum levels of FSH, LH/FSH, TG, TC, LDL-C and other related factors. The results of H&E staining of ovarian tissues showed that the ACP group showed different degrees of improvement in the reduced number of corpus luteum, thinning of granulosa cell layer, follicular capsular dilatation and follicular atresia. ACP not only reduces BW and the ratio of ovarian to BW, but also it can elevate the level of FSH and reduce LH/FSH, TG, TC and HDL-C levels. Furthermore, ACP can protect ovarian tissue to some extent.

N-isopropyl acrylamide/sodium acrylate hydrogel as draw agent for forward osmosis to concentrate esterification wastewater

Yan Le,Yanbin Yun,왕만상,Wenli Liu,Shuangshuang Dong,Kai Yang,Syed Taj Ud Din,Woochul Yang,Guicheng Liu 한국화학공학회 2021 Korean Journal of Chemical Engineering Vol.38 No.5

In recent years, a temperature-sensitive hydrogel was reported as a promising draw agent in forward osmosis (FO) technology. PEG, acts as porogen, as an enabler to improve the swelling property of hydrogels. From FO test, the addition of porogen to the hydrogel can improve the water flux of FO by increasing the swelling properties of the hydrogel. And the hydrogel modified with porogen improves the concentration efficiency of wastewater from 1.09 to 1.124 times, indicating that the modification of the hydrogel by the porogen has positive significance for FO technology. In this study, an advanced hydrogel was synthesized via physical copolymerization by using N-isopropylacrylamide and sodium acrylate. The internal structure was investigated by SEM test where it was found that that porogens have different mechanisms of action on hydrogel performance: Porogen affects the swelling property of hydrogel by changing the internal network structure through physical “occupation”. The effect of porogen concentration is to act on the porosity of hydrogel, while the main effect of the molecular weight of porogen on the hydrogel structure is by altering the pore size.