东亚地区侵权法实现一体化的基础及研究任务

杨立新(Yang LiXin) 경희법학연구소 2010 경희법학 Vol.45 No.4

Taurocholic acid promotes hepatic stellate cell activation via S1PR2/p38 MAPK/YAP signaling under cholestatic conditions

Jing Yang,Xujiao Tang,Zhu Liang,Mingzhu Chen,Lixin Sun 대한간학회 2023 Clinical and Molecular Hepatology(대한간학회지) Vol.29 No.2

Background/Aims: Disrupted bile acid regulation and accumulation in the liver can contribute to progressive liver damage and fibrosis. However, the effects of bile acids on the activation of hepatic stellate cells (HSCs) remain unclear. This study investigated the effects of bile acids on HSC activation during liver fibrosis, and examined the underlying mechanisms. Methods: The immortalized HSCs, LX-2 and JS-1cells were used for the in vitro study. in vitro, the adeno-associated viruses adeno-associated virus-sh-S1PR2 and JTE-013 were used to pharmacologically inhibit the activity of S1PR2 in a murine model of fibrosis induced by a 3,5-diethoxycarbonyl-1,4-dihydrocollidine (DDC) diet. Histological and biochemical analyses were performed to study the involvement of S1PR2 in the regulation of fibrogenic factors as well as the activation properties of HSCs. Results: S1PR2 was the predominant S1PR expressed in HSCs and was upregulated during taurocholic acid (TCA) stimulation and in cholestatic liver fibrosis mice. TCA-induced HSC proliferation, migration and contraction and extracellular matrix protein secretion were inhibited by JTE-013 and a specific shRNA targeting S1PR2 in LX-2 and JS-1 cells. Meanwhile, treatment with JTE-013 or S1PR2 deficiency significantly attenuated liver histopathological injury, collagen accumulation, and the expression of fibrogenesis-associated genes in mice fed a DDC diet. Furthermore, TCAmediated activation of HSCs through S1PR2 was closely related to the yes-associated protein (YAP) signaling pathway via p38 mitogen-activated protein kinase (p38 MAPK). Conclusions: TCA-induced activation of the S1PR2/p38 MAPK/YAP signaling pathways plays a vital role in regulating HSC activation, which might be therapeutically relevant for targeting cholestatic liver fibrosis.

Inactivation of MuxABC-OpmB Transporter System in Pseudomonas aeruginosa Leads to Increased Ampicillin and Carbenicillin Resistance and Decreased Virulence

Liang Yang,Lin Chen,Lixin Shen,Michael Surette,Kangmin Duan 한국미생물학회 2011 The journal of microbiology Vol.49 No.1

Resistance-Nodulation-Cell Division (RND) pumps play important roles in bacterial resistance to antibiotics. Pseudomonas aeruginosa is an important human pathogen which exhibits high level resistance to antibiotics. There are total of 12 RND pumps present in the P. aeruginosa PAO1 genome. The recently characterized MuxABC-OpmB system has been shown to play a role in resistance to novobiocin, aztreonam, macrolides,and tetracycline in a multiple knockout mutation. In this study, we examined the expression levels of all the 12 RND pump gene clusters and tested the involvement of MuxABC-OpmB in pathogenicity. The results indicated that in addition to the four known constitutively expressed RND pumps, mexAB-oprM, mexGHIopmD,mexVW, and mexXY, relatively high levels of expression were observed with mexJK and muxABCopmB in the conditions tested. Inactivation of muxA in the muxABC-opmB operon resulted in elevated resistance to ampicillin and carbenicillin. The mutant also showed attenuated virulence in both Brassica rapa pekinensis and Drosophila melanogaster infection models. The decreased virulence at least in part was due to decreased twitching motility in the mutant. These results indicate that the RND pump MuxABC-OpmB is associated with ampicillin and carbenicillin susceptibility and also involved in pathogenesis in P. aeruginosa.

Multiple Node Flip Fast-SSC Decoding Algorithm for Polar Codes Based on Node Reliability

Guo Rui,Yang Pei,Ying Na,Wang Lixin 한국인터넷정보학회 2022 KSII Transactions on Internet and Information Syst Vol.16 No.2

This paper presents a fast-simplified successive cancellation (SC) flipping (Fast-SSC-Flip) decoding algorithm for polar code. Firstly, by researching the probability distribution of the number of error bits in a node caused by channel noise in simplified-SC (SSC) decoder, a measurement criterion of node reliability is proposed. Under the guidance of the criterion, the most unreliable nodes are firstly located, then the unreliable bits are selected for flipping, so as to realize Fast-SSC-Flip decoding algorithm based on node reliability (NR-Fast-SSC-Flip). Secondly, we extended the proposed NR-Fast-SSC-Flip to multiple node (NR-Fast-SSC-Flip-ω) by considering dynamic update to measure node reliability, where ω is the order of flip-nodes set. The extended algorithm can correct the error bits in multiple nodes, and get good performance at medium and high signal-to-noise (SNR) region. Simulation results show that the proposed NR-Fast-SSC-Flip decoder can obtain 0.27dB and 0.17dB gains, respectively, compared with the traditional Fast-SSC-Flip [14] and the newly proposed two-bit-flipping Fast-SSC (Fast-SSC-2Flip-E2) [18] under the same conditions. Compared with the newly proposed partitioned Fast-SSC-Flip (PA-Fast-SSC-Flip) ( =4) [18], the proposed NR-Fast-SSC-Flip-ω (ω=2) decoder can obtain about 0.21dB gain, and the FER performance exceeds the cyclic-redundancy-check (CRC) aided SC-list (CRC-SCL) decoder (L=4).

A Robust Mesh Growing Surface Reconstruction Algorithm based on Octree

Liu Xumin,Yang Lixin,Li Cailing 보안공학연구지원센터(IJSIP) 2014 International Journal of Signal Processing, Image Vol.7 No.3

DDAB-MODIFIED TPGS-b-(PCL-ran-PGA) NANOPARTICLES AS ORAL ANTICANCER DRUG CARRIER FOR LUNG CANCER CHEMOTHERAPY

TIEJUN ZHAO,HEZHONG CHEN,LIXIN YANG,HAI JIN,ZHIGANG LI,LIN HAN,FANGLIN LU,ZHIYUN XU 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2013 NANO Vol.8 No.2

Oral chemotherapy is a great way to cancer treatment because it is less stressful being that the patient will have less hospital visits and can still maintain a close relationship with health care professionals. In this research, three types of nanoparticle formulation from commercial PCL and self-synthesized TPGS-b-(PCL-ran-PGA) diblock copolymer were fabricated for oral delivery of antitumor agents, including DDAB-modified PCL nanoparticles, unmodified TPGS-b-(PCL-ran-PGA) nanoparticles and DDAB-modified TPGS-b-(PCL-ran-PGA) nanoparticles. Firstly, the TPGS-b-(PCL-ran-PGA) diblock copolymer was synthesized and characterized. DDAB was adopted to increase retention time at the cell surface, thus increasing the chances of nanoparticle uptake by the gastrointestinal mucosa and improving drug absorption. The TPGS-b-(PCL-ran-PGA) nanoparticles were found by FESEM of spherical shape and around 200 nm in diameter. The surface charge of TPGS-b-(PCL-ran-PGA) nanoparticles was reversed from anionic to cationic after DDAB modification. The DDAB-modified TPGS-b-(PCL-ran-PGA) nanoparticles have significantly higher level of the cell uptake than that of DDAB-modified PCL nanoparticles and unmodified TPGS-b-(PCL-ran-PGA) nanoparticles. In vitro cell viability studies showed advantages of the DDAB-modified TPGS-b-(PCL-ran-PGA) nanoparticles over Taxotere® in terms of cytotoxicity against A549 cells. In conclusion, oral chemotherapy by DDAB-modified TPGS-b-(PCL-ran-PGA) nanoparticle formulation may provide a promising outcome for lung cancer patients.

Pyramid-like CdS nanoparticles grown on porous TiO₂ monolith: An advanced photocatalyst for H₂ production

Du, Jimin,Wang, Huiming,Yang, Mengke,Li, Kaidi,Zhao, Lixin,Zhao, Guoyan,Li, Sujuan,Gu, Xiaolei,Zhou, Yalan,Wang, Le,Gao, Yating,Wang, Weimin,Kang, Dae Joon Pergamon Press 2017 Electrochimica Acta Vol. No.

<P><B>Abstract</B></P> <P>Efficient production of H<SUB>2</SUB> via solar-light-driven water splitting by a semiconductor-based photocatalyst without noble metals is crucial owing to increasingly severe global energy and environmental issues. However, many challenges, including the low efficiency of H<SUB>2</SUB> evolution, low solar light absorption, excited electron–hole pair recombination, and slow transport of photoexcited carriers, must be resolved to enhance the H<SUB>2</SUB> photoproduction efficiency and photocatalyst stability. Here, a two-step method is used to synthesize advanced H<SUB>2</SUB>-generating photocatalysts consisting of pyramid-like CdS nanoparticles grown on a porous TiO<SUB>2</SUB> monolith, which show promising photocatalytic activity for the hydrogen evolution reaction. Furthermore, the stability of the photocatalysts is examined through long-term tests to verify their good durability. Without noble metals as cocatalysts, the photocatalyst can reach a high H<SUB>2</SUB> production rate of 1048.7μmolh<SUP>−1</SUP> g<SUP>−1</SUP> under UV–vis irradiation when the ratio of the CdS nanoparticles to TiO<SUB>2</SUB> is 5mol%. This unusual photocatalytic activity arises from the wide-region light adsorption due to the narrow band gap of CdS, effective separation of electrons and holes due to conduction band alignment at the CdS–TiO<SUB>2</SUB> interface, and favorable reaction sites resulting from the porous structure.</P>

Identification and characterization of a novel goose-type and chickentype lysozyme genes in Chinese rare minnow (Gobiocypris rarus) with potent antimicrobial activity

Yingying Zhang,Hui Yang,Weijia Song,Dan Cui,Lixin Wang 한국유전학회 2018 Genes & Genomics Vol.40 No.6

Lysozymes act as important innate immunity molecule against various pathogen infections. In the present study, a new c-type and g-type lysozymes were isolated from rare minnow Gobiocypris rarus. The deduced amino acid sequence of c-type lysozyme contained 145 residues and possessed conserved catalytic residues ( Glu53 and Asp72). The deduced g-type lysozyme contained 185 residues and possessed three conserved catalytic residues ( Glu73, Asp86 and Asp97). qPCR analysis revealed that GrlysG and GrlysC were constitutively expressed in all examined tissues. GrlysG was most abundant in kidney and liver, while GrlysC was predominantly expressed in liver. The transcripts of GrlysG and GrlysC genes could be significantly up-regulated after Aeromonas hydrophila and poly I:C infection in the kidney and liver. The recombinant GrlysG and GrlysC proteins were successfully produced and purified. The optimal pH and temperature for rGrlysG and rGrlysC protein lytic activities was determined to be 6.5 and 32 °C, respectively. From the minimal inhibitory concentration test, the rGrlysG and rGrlysC exhibited apparent antibacterial activities against both Gram-positive and Gram-negative bacteria at different concentrations. In conclusion, the present study indicated that c-type and g-type lysozymes participated the innate immune response to A. hydrophila and virus infections in fish.

Effects of actinobacteria on plant disease suppression and growth promotion

Palaniyandi, Sasikumar Arunachalam,Yang, Seung Hwan,Zhang, Lixin,Suh, Joo-Won Springer-Verlag 2013 Applied microbiology and biotechnology Vol.97 No.22

<P>Biological control and plant growth promotion by plant beneficial microbes has been viewed as an alternative to the use of chemical pesticides and fertilizers. Bacteria and fungi that are naturally associated with plants and have a beneficial effect on plant growth by the alleviation of biotic and abiotic stresses were isolated and developed into biocontrol (BCA) and plant growth-promoting agents (PGPA). Actinobacteria are a group of important plant-associated spore-forming bacteria, which have been studied for their biocontrol, plant growth promotion, and interaction with plants. This review summarizes the effects of actinobacteria as BCA, PGPA, and its beneficial associations with plants.</P>

The Mathematic Model of "Pressing Complexion" Differential Coefficient Countermeasure Decision for Collision-avoidance

Cai Feng,Shi Aiguo,Yang Baozhang,Zhou Lixin 한국항해항만학회 2001 한국항해항만학회 학술대회논문집 Vol.3 No.-

In this article, we have done some analysis about the collision-avoidance specialty of "pressing complexion" and "pressing danger" in the meet process of two boats, and offered a mathematic model of differential coefficient countermeasure decision for collision-avoidance. which adapt to the right complexion. The basal idea is, in the right condition whatever do the coming boat do, and our boat will always adopt dynamic, continuous and the best countermeasure. When both the controlling capabilities of two boats have advantage and inferior position, we can working-out with the qualitative differential coefficient countermeasure.