Cloing and Expression of Bdi Ⅰ Methylase Gene in E . coli

노현모,김용석,최경래 한국유전학회 1984 Genes & Genomics Vol.6 No.3

The gene for the Bdi I modification enzyme, which is a counterpart of Bdi I restriction endonuclease, from Brevibacterium divaricatum FERM 5948 was cloned and expressed in E. coli. First, we have isolated Bdi I restriction endonuclease by cleavage assay in vitro with three column steps and have proved that Bdi I restriction enzyme is an isoschizomer of Cla I whose recognition sequence is 5' ATCGAT 3'. For cloning of Bdi I methylase gene, we have initially used three cloning sites (Sal I, Bam HI and Eco RI) of pBR 322 and adopted the retransformation method after Bdi I restriction endonuclease cleavage. Selection of transformants carrying the gene was based on the resistance of the modified plasmid encoding the enzyme to cleavage by Bdi I restriction enzyme. Among colonies after retransformation, the recombinant plasmid, pBDIM 116 containing 5.2 Kb EcoRI insert was proved to carry the gene. Using the restriction map constructed, we inserted λ-Sal I fragment containing another Bdi I site into pBDIM 116 and subcloned various DNA fragment for Bdi I methylase gene mapping. The expression of Bdi I methylase gene in E. coli and the relationship between Bdi I restriction-modification genes will be also discussed.

Isolation of polyunsaturated compounds showing anti-inflammatory effect from the marine sponge Axinella rugosa

Kyung Lee,ah-ram Lee,Jung-Rae Rho,Jung-ja Sim,Ok-Hwa Kang 한국해양환경·에너지학회 2012 한국해양환경공학회 학술대회논문집 Vol.2012 No.5

Effect of Oranic Fertilizer Application on Growth Characteristics and Saponin Content in Codonopsis lanceolata

Kyung Gon Mun(문경곤),Min Ju Lee(이민주),Young Guk Kim(김영국),Yeong Son Cho(조영손),Il Rae Rho(노일래) 한국약용작물학회 2017 한국약용작물학회 학술대회논문집 Vol.2017 No.1

Isolation of polyunsaturated compounds showing anti-inflammatory effect from the marinesponge Axinella rugosa

kyung Lee,ah-ram Lee,Jung-Rae Rho,Jung-ja Sim,Ok-Hwa Kang 대한조선학회 2012 대한조선학회 학술대회자료집 Vol.2012 No.5

소아 호흡기 감염 치료에 있어서의 Zithromax^(�)의 유효성 및 안전성에 관한 연구

노영일,박동호,문경래,박영봉 최신의학사 1995 最新醫學 Vol.38 No.7

이중 박동형 생명 구조장치의 개발(T-Pls)

노양래,이혁수,황창모,최성욱,이정찬,최혁,이환성,오혜정,안상수,손호성,백광제,원용순,선경,최민주,민병구 제주대학교 인공심장이식연구소 2001 인공심장 연구 Vol.2 No.2

심혈관 질환 특히 협심증과 급성 심근 경색증 등 관상동맥 질환이 급증함에 따라 병원 밖이나 응급실 중환자실 심도자 검사실등에서 급성 심장 쇼크에 빠지거나 심장마비가 발생하는 경우가 많이 발생하고 있다. 이러한 심혈관 질환에는 정류성 체외 생명보조장치가 널리 사용되고 있으나 이러한 장치는 그 무게와 크기로 인해 응급상황에 대처할 수 있는 능력이 작고, 막형 산화기의 전단에 인가되는 압력이 크며, 시스템 구성상 혈액이 공기에 노출된다. 그리하여 박동성 혈류를 공급할 수 있는 공기에 노출되지 않는 시스템(Close System)이 필요하다. 박동성 혈류를 공급하기 위하여 단일 구동펌프를 이용한 시도가 있었으나 회로내의 순간 압력 상승등으로 인한 단점이 도출되었다. 이러한 기존의 체외생명보조장치의 단점을 극복하고자, 한국형 인공심장의 원리를 이용한 박동식 이중 구동형 혈액펌프를 개발하였고, T-Pls(Twin-Pls)라 명칭하였다. 이 시스템의 구동원리는 막형산화기를 중심으로 전 ㆍ 후에 혈액펌프를 위치하여 혈액펌프를 Actuator가 한번씩 짜주는 것이다. 이런 방법으로 구동할 경우 막형산화기 후단에 있는 펌프가 음압을 동시에 일으킴으로, 막형산화기에 인가되는 큰 양압을 줄인다는 가정하에 이 시스템을 개발하였고, in vitro 실험을 통하여 각 부분의 압력을 측정하고, 총 12건의 동물실험(대형동물 : 6건, 소형동물 : 6건)을 통하여 그 장점을 검증하였다. Many cases of Acute cardiac shock and Cardiac arrest in Emergency room and ICU have been increasing. In this case, ECMO with Centrifugal pump has been used generally. However, due to the heavy weight and big size, the system is not adequate for Emergency cases. And other defects of this system are that Membrane Oxygeantor's pressure is high and blood are exposed to the air. There was some tries of ECMO using pulsatile pump, but it was found that the weak point of these system is high peak pressure and hemolysis. To overcome these defects, we have developed Twin Pulsatile Life System and we named 'T-Pls'. The mechanism of 'T-Pls' is that Membrane oxygenator Outlet Pump(MOP) make negative pressure when Membrane oxygenator Inlet Pump(MIP) provides high positive pressure, and the negative pressure will decrease positive pressure of Membrane Oxygeantor. Our group analyzed this advantage through In-vitro and 12 Cases In- Vivo test(Big animal : 6 cases, small animal : 6 cases).

더덕의 유기질 비료 시용이 생육 및 사포닌 함량에 미치는 영향

Kyung Gon Mun,Min Ju Lee,Young Guk Kim,Yeong Son Cho,Il Rae Rho 한국약용작물학회 2017 한국약용작물학술대회 발표집 Vol.2017 No.05

Background : Codonopsis lanceolata is currently used as vegetable, as well as materials for traditional medicines. However, consumers have nagative views on using pesticides and chemical fertilizer in C. lanceolata cultivation. Therefore, this research was conducted to select the appropriate organic fertilizer to improve the growth and saponin components of C. lanceolata by some organic fertilizers application. Methods and Results : Organic fertilizers were applied as 4 types: mixed organic matter, fermentation cake, bacterial culture and rice husks, excluding conventional chemical fertilizer and non-treatment used as control. The result analyzed in soil after fertilizer application showed that soil pH was acidified in fermentation cake and chemical fertilizer treatment, especially, chemical fertilizer treatment showed very high phosphoric-acid content than other treatments, and total N content was higher in fermentation cake, mixed organic matter and chemical fertilizer. Growth of C. lanceolata showed superior tendency in the treatment of mixed organic matter and fermentation cake. Lancemacides could be identified as foetidissimoside A, lancemaside A, lancemaside B, and lancemaside D. However, among them, quantitative analysis could not be conducted on foetidissimoside A due to its very low content, and lancemaside A was the most abundant saponin in the root from all the treatments. The content of lancemaisde A according to organic fertilizer application showed the highest value in the treatment of mixed organic matter, followed by the fermentation cake, bacterial culture, non-treatment, rice husks and chemical fertilizer, in that order. The content of lancemaside B and D was very low compared to lancemaside A, and there was no difference among treatments. Conclusion : The growth of C. lanceolata was superior in application of mixed organic matter and fermentation cake, and the major saponin, lancemaside A, was also increased.

A new sesterterpenoid showing anti-inflammatory effect from the Marine Sponge Haliclona species

Lee, Kyung,Rho, Jung-Rae Korean Magnetic Resonance Society 2015 Journal of the Korean Magnetic Resonance Society Vol.19 No.1

Four spiroketal sestertepenoids (1 ~ 4) were isolated from the marine sponge Haliclona species. Their planar structures were completely determined from a combination of extensive 1D and 2D NMR experiments, and also the relative stereochemistry on the chiral centers were established by the ROESY experiment. Compounds 1 ~ 3 were determined as the same planar structures with different stereochemistry on the chiral centers C-11 and C-13. Of these, 1 was identified as a new stereoisomer. Four compounds showed the inhibition effect of nitric oxide (NO) production in LPS-stimulated RAW 264.7 cells (0.7~2.0 g/ml).

한국형 인공심장에 대한 동물실험(AnyHeart™)

선경,손호성,이성호,신재승,이혜원,김승철,원용순,이경갑,정종태,김종원,노양래,이혁수,민병구 제주대학교 인공심장이식연구소 2001 인공심장 연구 Vol.2 No.2

말기심부전증의 새로운 치료대안으로 제시되는 한국형 인공심장(AnyHeartTM)의 효용성과 안정성을 평가하기 위하여 동물실험을 실시하였다. 지난 2년간 고려대학교 안암병원과 제주대학교 동물병원에서는 총 28마리(송아지 18, 면양 4, 실험견 3, 말 2, 산양 1마리)의 다양한 동물에서 한국형 인공심장 이식실험이 실시되었으며, 좌심보조장치로 적용한 1예와 체외휴대형으로 적용한 각 1예를 제외하고 모두 심장보존형 인공심장(이식형 양심보조장치)의 형태로 완전 이식하였다. 수술방법은 주로 우측개흉법을 (Korea University Technique)을 사용하였다. 펌프 박출량은 최대 6 L/min로 평균 3-3.5 L/min를 유지되었으며, Fitting test 4례와 초기 적응과정의 2마리를 제외하고 모두 수술에서 회복되었다. 최장 관찰기간은 37일로 합병증 없이 건강한 상태에서 기초자료 확보를 위해 관찰을 임의종료하였다. 모든 생존동물에서 기계장치와 관련된 부작용은 발견되지 않았고, 임상적으로 나타나는 혈전전색 소견이나 간기능, 신기능, plasma hemoglobin, fibrinogen 등에 이상은 없었다. 적절하게 조기부검이 이루어진 경우에는 인공심장 내부에 혈전이 발견되지 않았고, 간/콩팥/비장 등의 중요장기에 병리검사에서도 전색소견은 발견되지 않았다. 결론으로, 한국형 인공심장은 생체 내에 이식하였을 때 안정적이며 기능이 우수한 것을 알 수 있었다. 한국형 인공심장은 현 상태에서 임상에 적용할 수 있다고 판단되며, 가까운 시일 내에 인체 내 안정성와 유효성에 대한 평가가 가능할 것으로 기대한다. Korean artificial heart (AnyHeartTM) is a new vision in the management of end stage heart failure. We performed the animal experiments to evaluate the performance and safety of AnyHeart. For last 2 years, 28 various animals (cattle 18, sheep 4, canine 3, goat 1) underwent AnyHeart implantation at Korea University Medical Center and Cheju University Animal Hospital. AnyHeart was applied as an implantable BVAD except a case of implantable LVAD or wearable BVAD. A standard technique of implantation was a right thoracotomy approach (Korea University Technique). The pump output was 6 L/min in maximum and 3-3.5 L/min in average. Other than 4 cases of fitting tests and 2 in the early learning period, all animals were recovered from the operation. The longest observation was made until 37th postoperative day, and the observation was terminated to get a control data while the animal was healthy without complications. All survivors were free from device-related morbidity, and there were no evidence of clinically significant thromboembolic episode, liver functions, renla function, plasma hemoglobin, fibrinogen abnormalities. Appropriate autopsy did not show thrombus in the device or embolism in the organs. Conclusively, AnyHeart has been proved efficient and safe during in-vive implantation. The authors believe that AnyHeart can be applied to clinical situations at the moment and expect to perform a clinical trial to test safety and efficacy in the human body within near future.

통원 백내장 수술 후 개별 교육이 불안과 자가관리 수행에 미치는 영향

조경숙,노창래,Kyung-Sook Cho,Chang Rae Rho 대한안과학회 2012 대한안과학회지 Vol.53 No.3

Purpose: To investigate the influence of additional postoperative individualized teaching on the anxiety levels and self-care compliance of postoperative care in patients after one-day cataract surgery. Methods: The present study included 80 cataract patients who underwent phacoemulsification and posterior intraocular lens insertion performed by a single operator between January 2011 and February 2011. The control group consisted of patients who received postoperative education from an ophthalmology coordinator based on previous hospital policy. The experiment group consisted of patients who received additional individualized postoperative education from an operator in the recovery room. Trait anxiety on operation day, state anxiety on postoperative day 1, and self-care compliance and postoperative satisfaction at postoperative 1 month were evaluated. Results: There were no significant differences of trait anxiety levels on operation day or state anxiety on postoperative day 1 between the control and experiment groups. The experiment group revealed a higher score on the postoperative self-care compliance questionnaire, especially regarding care of the operation site. Conclusions: Individualized postoperative education increases self-care compliance level and contributes to improve postoperative recovery after one-day cataract surgery. J Korean Ophthalmol Soc 2012;53(3):412-419