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Effective Methods to Improve the Biocompatibility of Poly(dimethylsiloxane)
박중열,황창모,이상훈 한국바이오칩학회 2008 BioChip Journal Vol.2 No.1
Poly (dimethylsiloxane) (PDMS) has become one of the popular materials in the field of bio-related microfluidic systems, and has been used for various biological assays. While the material is known to be biocompatible in general, it also has been reported that the attachment and survival rate of cells are limited on a PDMS substrate. In this paper, a simple and relevant method to improve heiocompatibilityof PDMS is proposed; the effects of various treatments using ethanol, water, and boiling-water were evaluated. The results show that the boiling-water treatment is the most appropriate, time saving, and simple method to improve the biocompatibility of PDMS.
세포 함유 젤라틴 파이버 응용을 통한 골 재생 유도용 인산칼슘 생체재료 세포 탑재 연구
김선화,황창모,박상혁 대한의용생체공학회 2022 의공학회지 Vol.43 No.1
Natural and synthetic forms of calcium phosphate cement (CPC) have been widely used in bone repair and augmentation. The major challenge of injectable CPC is to deliver the cells without cell death in order to regen- erate new bone. The study objective was to investigate for the potential of stem cell-laden gelatin fibers containing injectable, nanocrystalline CPC to function as a delivery system. Gelatin noddle fiber method was developed to deliv- ered cells into nCPC. Experimental groups were prepared by mixing cells with nCPC, mixing cell-laden gelatin fibers with nCPC and mixing cell-laden gelatin fibers containing BMP-2 with nCPC. Media diffusion test was conducted after dissolving the gelatin fibers. SEM examined the generated channels and delivered cell morphology. Fibers mixed with nCPC showed physical setting and hardening within 20 min after injection and showed good shape main- tenances. The gelatin fibers mixed nCPC group had several vacant channels generated from the dissolved gelatin. Particularly, proliferation and attachment of the cells were observed inside of the channels. While live cells were not observed in the cell mixed nCPC group, cells delivered with the gelatin fibers into the nCPC showed good viability and increased DNA content with culture. Cell-laden gelatin fiber was a novel method for cell delivery into nCPC with- out cell damages. Results also indicated the osteogenic differentiation of gelatin fiber delivered cells. We suggest that the cell-laden gelatin fibers mixed with nCPC can be used as an injectable cell delivery vehicle and the addition of BMP-2 to enhances osteogenesis.
선경,황창모,이정주,박용두,이규백,곽영태 한국생체재료학회 2005 생체재료학회지 Vol.9 No.1
The primary function of natural heart is pumping blood to the whole body and the pulmonary circulation. The heart beats 100,000 times a day and around 35 million times a year. Pumping blood by heart supplies nutrients and oxygen to the essential organs and tissues. When the heart function is deteriorated, heart patients suffer from various side effects. Heart transplantation is the only approach in the clinical treatments. However, the number of donor heart is quite small compared to the required heart for transplantation. In 2001, 700,000 deaths are directly caused by heart disease in U.S.A. and there are 5,000,000 congestive heart failure patients. Moreover, 550,000 new congestive heart failure patients are diagnosed and 55,000 patients are died of congestive heart failure. There are 200,000 heart disease patients who are out of the conventional medical treatments. Therefore, new approaches substituting failing heart are required for the dying patients. Since late 1950s, the artificial heart development program for substituting natural heart was launched aiming recovering heart function and longer survival of the patients. This paper reviews the brief history of artificial heart development and the current status of artificial hearts which are on clinical application or under development.
진공성형을 이용한 삼엽식 고분자 심장판막의 제작과 혈류역학적 성능평가
김경현,황창모,정기석,안치범,김범수,이정주,남경원,선경,Kim, K.H.,Hwang, C.M.,Jeong, G.S.,Ahn, C.B.,Kim, B.S.,Lee, J.J.,Nam, K.W.,Sun, K. 대한의용생체공학회 2006 의공학회지 Vol.27 No.6
In the artificial heart application, productivity and hemodynamic properties of artificial heart valves are crucial in successiful application to long term in vivo trials. This paper is about manufacture and assessment of trileaflet polymer heart valves using vacuum forming process(VFP). The VFP has many advantages such as reduced fabrication time, reproducibility due to relatively easy and simple process for manufacturing. Prior to VFP of trileaflet polymer heart valves, polyurethane(Pellethane 2363 80AE, Dow Chemical) sheet was prepared by extrusion. The sheets were heated and formed to mold shape by vacuum pressure. The vacuum formed trileaflet polymer heart valves fabrication is composed of two step method, first, leaflet forming and second, conduit forming. This two-step forming process made the leaflet-conduit bonding stable with any organic solvents. Hydrodynamic properties and hemocompatibility of the vacuum formed trileaflet polymer heart valves was compared with sorin bicarbon bileaflet heart valve. The percent effective orifice area of vacuum formed trileaflet polymer heart valves was inferior to bileaflet heart valve, but the increase of plasma free hemoglobin level which reflect blood damage was superior in vacuum formed trileaflet polymer heart valves Vacuum formed trileaflet polymer heart valves has high productivity, and superior hemodynamic property than bileaflet heart valves. Low manufacturing cost and blood compatible trileaflet polymer heart valves shows the advantages of vacuum forming process, and these results give feasibility in in vivo animal trials in near future, and the clinical artificial heart development program.
Multilayered Engineered Tissue Sheets for Vascularized Tissue Regeneration
홍소영,정보영,황창모 한국조직공학과 재생의학회 2017 조직공학과 재생의학 Vol.14 No.4
A major hurdle in engineering thick and laminated tissues such as skin is how to vascularize the tissue. This study introduces a promising strategy for generatingmulti-layering engineered tissue sheets consisting of fibroblasts and endothelial cells co-seeded on highly micro-fibrous, biodegradable polycaprolactone membrane. Analysis of the conditions for induction of the vessels in vivo showed that addition of endothelial cell sheets into the laminated structure increases the number of incorporated cells and promotes primitive endothelial vessel growth. In vivo analysis of 11-layered constructs showed that seeding a high number of endothelial cells resulted in better cell survival and vascularization 4 weeks after implantation.Within one week after implantation in vivo, red blood cells were detected in the middle section of three-layered engineered tissue sheets composed of polycaprolactone/ collagen membranes. Our engineered tissue sheets have several advantages, such as easy handling for cell seeding, manipulation by stacking each layer, a flexible number of cells for next-step applications and versatile tissue regeneration, and automated thick tissue generation with proper vascularization.