Meeting report: The biology of genomes and proteomes

Han, Kyudong,Jin, Han Jun,Kim, Heui-Soo,Kim, Nam-Soo Springer-Verlag 2015 Genes & Genomics Vol.37 No.7

A Proposal of Performance Indicator of Lane Keeping Assist System (LKAS)

Kyudong Park,Sung H. Han,Jiyoung Kwahk,Minseok Song,Dong Gu Choi,Hyeji Jang,Dohyeon Kim,Young Deok Won,In Sub Jeong 대한인간공학회 2017 대한인간공학회 학술대회논문집 Vol.2017 No.4

The main purpose of this study is to derive the performance indicator of the LKAS (Lane Keeping Assist System) in consideration of the sense of heterogeneity felt by the driver, and to propose the necessary measurement data for an objective evaluation. As consumers’ interest in automobile safety has increased, research on Advanced Driver Assistance System (ADAS) has been actively conducted worldwide. There is a need to consider the user experience on the driver’s side in future development direction. The heterogeneity was reenacted and observed using a test vehicle that can change the detailed settings of LKAS, and the heterogeneity was summarized through workshops and interviews with ADAS performance evaluation experts. Based on this, four performance indicators (lane keeping performance, vehicle behavior stability, steering stability, driver non-interference performance) were derived. In addition, the measurement data for each indicator was proposed. A program that automatically collects the proposed measurement data and supports the statistical analysis is under development, and it is expected to be useful for LKAS performance evaluation in the future.

Touch or Push?: Button Design for VR HMD using Hand Interaction

Kyudong Park,Dohyeon Kim,Sung H. Han 대한인간공학회 2018 대한인간공학회 학술대회논문집 Vol.2018 No.5

Virtual reality (VR) enhances immersion by allowing users to view and manipulate virtual situations, landscapes, or objects through devices such as HMDs (Head-Mounted Displays). The aim of this study is to investigate the effects of button depth on the usability of virtual buttons in VR environments using hand interaction. The experiment was conducted in a desktop setting with Oculus Rift and Leap motion, and was designed with a balanced Latin Square. A total of 18 subjects performed a button selection task. Task completion time, number of errors, and subjective preferences were used to test different levels of button depth. The touch method that has 0-mm depth took a shorter task completion time with higher scores of subjective preferences. However, the push method that has 10-mm depth recorded a smaller number of errors, due to the visual push-feedback. The results can be applied to many different application areas.

An Eye Movement Analysis with Process Mining Techniques for Usability Evaluation

Kyudong Park,Sung H. Han 대한인간공학회 2017 대한인간공학회 학술대회논문집 Vol.2017 No.11

The main purpose of this study is to propose an analysis and visualization method of eye movement that can be employed for usability evaluation. Eye tracking in usability evaluation has become more common over the past few years. A gaze plot and a heat map are popularly used to visualize the pattern of gaze movements, but it is difficult to integrate the gaze data of many subjects. We proposed a new method with process mining techniques, and a case study was conducted on K-MOOC website with 12 participants. As a result, a process model was derived from the event log based on the eye movements, and a gaze movement pattern could be obtained by using the developed model. We also found potential usability problems through simulation of the model and the bottlenecks. The proposed method might help to provide novel insights in usability evaluation.

차선 유지 보조 시스템으로 인해 발생하는 이질감의 계측에 대한 연구

박규동(Kyudong Park),곽지영(Jiyoung Kwahk),한성호(Sung H. Han),송민석(Minseok Song),최동구(Dong Gu Choi),장혜지(Hyeji Jang),김도현(Dohyeon Kim) 대한인간공학회 2018 大韓人間工學會誌 Vol.37 No.4

Objective: The objective of this study is to investigate the intrusive feeling caused by a Lane Keeping Assistance System (LKAS) and measure it objectively through the sensor log data of a vehicle. Background: LKAS, one of the Advanced Driver Assistance System (ADAS), directly intervenes in the steering wheel control to assist the driver to keep the lane. Due to the torque generated by the LKAS, the driver has a different and undesirable feeling from a usual situation, which can affect the driving experience. Nevertheless, the performance of the LKAS in most studies focused only on how well the system keeps the lane. Method: Through an actual driving test using a test vehicle equipped with an LKAS, two ADAS experts and two human factor experts observed the intrusive feeling while collecting sensor log data at the same time. After the types of the intrusive feeling were classified, variables that could account for the feeling were derived. Then, this study compared the data collected from the vehicle with and without the intrusive feeling. Results: Three types of the intrusive feeling were observed: abrupt lateral change, steering wheel vibration, and heavy steering. The lateral speed (LS), the high-pass filtered steering angle (FSA), and the interrupt torque (IT) were then derived as the variables that could explain each feeling, respectively. It is found that the patterns of the derived variables are different in driving with and without the intrusive feeling. Conclusion and Application: The intrusive feeling found in this study can be used as a performance index in the development stage of the LKAS. It will be helpful to systematically reduce the intrusive feeling.

Influence of yeast hydrolysate supplement on growth performance, nutrient digestibility, microflora, gas emission, blood profile, and meat quality in broilers

( Vetriselvi Sampath ),( Kyudong Han ),( In Ho Kim ) 한국축산학회(구 한국동물자원과학회) 2021 한국축산학회지 Vol.63 No.3

A total of 1512 Ross 308 broilers (one - day - old) were assigned (random blocks) to 1of 3 dietary treatments with 28 replicates of 18 chicks/cage. The dietary treatments were Corn-soybean-meal based basal diet supplemented with 0%, 0.1%, and 0.2% of commercial yeast hydrolysate (YH [Saccharomyces cerevisiae]). The graded level of YH supplementation has linearly increased broilers body weight gain on d 21, 35, and overall (p = 0.044, 0.029, and 0.036, respectively) experimental period. In addition, the increased level of YH supplementation has linearly reduced feed conversation ratio of broilers on d 21, 35, and overall trial period (p = 0.041, 0.052, and 0.032, respectively). However, the feed intake and mortality of broilers were not affected by the graded level of YH supplementation. Though nutrient digestibility of dry matter (p = 0.012) and nitrogen (p = 0.021) was linearly increased in broilers fed YH supplementation, at the end of the trial it fails to affect the total track digestible energy. Dietary inclusion of YH supplementation showed a beneficial effect on the microbial population as linearly improved lactobacillus (p = 0.011) and reduced Escherichia coli counts (p = 0.042). An increasing level of YH supplementation has tended to decrease NH3 (p = 0.069) and linearly decrease H2S (p = 0.027) of noxious gas emission in broilers. Moreover, dietary YH supplements trend to increase the glucose (p = 0.066) and reduced cholesterol (p = 0.069) level. At the end of the test, YH supplementation elicited a linear reduction in drip loss on days 5 and 7, respectively (p = 0.045, and 0.021). Furthermore, dietary inclusion of YH supplementation had linearly increased villus height (p = 0.051) but fails to affect crypt depth. Therefore, in terms of positive effects on the broiler’s overall performance, we suggest that dietary supplements containing graded YH levels in the broilers diet could serve as a potential alternative for growth promoters.

AbaR is a LuxR type regulator essential for motility and the formation of biofilm and pellicle in Acinetobacter baumannii

Man Hwan Oh,Kyudong Han 한국유전학회 2020 Genes & Genomics Vol.42 No.11

Background Acinetobacter baumannii is a major opportunistic pathogen causing nosocomial infections. Acinetobacter baumanniipossesses a quorum sensing system consisting of abaI, encoding an autoinducer synthase, and abaR, encoding aputative LuxR type regulator. AbaI is required for motility and biofilm formation in A. baumannii. However, the functionsof AbaR on the expression of abaI, motility, and the formation of biofilm and pellicle have not yet been explored. Objective The aim of this study was to investigate the effects of abaR mutation on the expression of abaI, motility, and theformation of biofilm and pellicle. Methods Functions of AbaR were assessed by the construction of an isogenic mutant and by evaluating the effects of abaRmutation on the expression of abaI, motility, and the formation of biofilm and pellicle. Results The abaR mutant revealed a significant decrease in the expression of abaI. The disruption of abaR resulted in substantialdefects in motility and the formation of biofilm and pellicle. Introduction of abaR in trans complemented the defects. Conclusions AbaR of A. baumannii is required for the expression of abaI and plays important roles in motility and the formationof biofilm and pellicle. AbaR may be considered to be a target of anti-biofilm agents.

A Genome-Wide Methylation Approach Identifies a New Hypermethylated Gene Panel in Ulcerative Colitis

Kang, Keunsoo,Bae, Jin-Han,Han, Kyudong,Kim, Eun Soo,Kim, Tae-Oh,Yi, Joo Mi MDPI AG 2016 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol.17 No.8

<P>The cause of inflammatory bowel disease (IBD) is still unknown, but there is growing evidence that environmental factors such as epigenetic changes can contribute to the disease etiology. The aim of this study was to identify newly hypermethylated genes in ulcerative colitis (UC) using a genome-wide DNA methylation approach. Using an Infinium HumanMethylation450 BeadChip array, we screened the DNA methylation changes in three normal colon controls and eight UC patients. Using these methylation profiles, 48 probes associated with CpG promoter methylation showed differential hypermethylation between UC patients and normal controls. Technical validations for methylation analyses in a larger series of UC patients (<I>n</I> = 79) were performed by methylation-specific PCR (MSP) and bisulfite sequencing analysis. We finally found that three genes (<I>FAM217B</I>, <I>KIAA1614</I> and <I>RIBC2</I>) that were significantly elevating the promoter methylation levels in UC compared to normal controls. Interestingly, we confirmed that three genes were transcriptionally silenced in UC patient samples by qRT-PCR, suggesting that their silencing is correlated with the promoter hypermethylation. Pathway analyses were performed using GO and KEGG databases with differentially hypermethylated genes in UC. Our results highlight that aberrant hypermethylation was identified in UC patients which can be a potential biomarker for detecting UC. Moreover, pathway-enriched hypermethylated genes are possibly implicating important cellular function in the pathogenesis of UC. Overall, this study describes a newly hypermethylated gene panel in UC patients and provides new clinical information that can be used for the diagnosis and therapeutic treatment of IBD.</P>

Comparison of genetic variation between primary colorectal cancer and metastatic peritoneal cancer

Shin Wonseok,Yun Jeongseok,Han Kyudong,Park Dong-Guk 한국유전학회 2023 Genes & Genomics Vol.45 No.8

Background Among cancer metastases by primary colorectal cancer (CRC), peritoneal metastasis is the second most common metastatic lesion after liver metastasis. In treating metastatic CRC, it is very important to differentiate targeted-therapy and chemotherapy according to the characteristics of each lesion because the genetic variation of the primary and metastatic lesions are different. However, there are few studies of genetic characteristics on peritoneal metastasis caused by primary CRC, so molecular-level studies are continuously required. Objective We propose an appropriate peritoneal metastasis treatment policy by identifying the genetic characteristics between primary CRC and synchronous peritoneal metastatic lesions. Methods Primary CRC and synchronous peritoneal metastasis samples were analyzed in pairs from six patients using Comprehensive Cancer Panel (409 cancer-related genes, Thermo Fisher Scientific, USA) and next-generation sequencing (NGS). Results The mutations were commonly found on the KMT2C and THBS1 genes in both primary CRC and peritoneal metastasis. The PDE4DIP gene was mutated in all cases except for on a sample of peritoneal metastasis. As a result of analysis using the mutation database, we confirmed that the gene mutations of primary CRC and the peritoneal metastasis derived from it showed the same tendency, although we did not accompany the gene expression level or epigenetic study. Conclusion It is thought that the treatment policy through molecular genetic testing of primary CRC can also be applied to peritoneal metastasis treatment. Our study is expected to be the basis for further peritoneal metastasis research.

Sound feedback of mid-air pointing gesture interaction with a head-mounted display

Bora Kang,Sung H., Han,Jiyoung Kwahk,Kyudong Park 대한인간공학회 2016 대한인간공학회 학술대회논문집 Vol.2016 No.11

The aim of this study is to verify the effectiveness of sound feedback and to improve usability of mid-air pointing gesture interaction with a head-mounted display. Recently, the mid-air gesture interaction on three-dimensional space has become more prevalent with the motion recognition technology. In the interaction, sounds have been actively utilized to provide various information to users. In an experiment, there were two independent variables: FEEDBACKS (no sound feedback / case-triggered sound feedback / continuous sound feedback with varying frequency / continuous sound feedback with varying duration) and TRACKS (curved / straight). The participants performed a path following task while keeping the center of tracks. The results showed that the continuous feedbacks coupled to pointing gesture helped the participants perform the task more accurate and finally get better performance in deviation because they could easily get information about deviation and could keep concentration on their task. The results of this study can be used to enhance user experience of gestural interaction with sound feedback in various areas: virtual reality, augmented reality and large-display interaction.