거대 수신증 3예

이규백,문장호,이순호,김세철 중앙대부설한국의과학연구소 1983 한국의과학 Vol.15 No.4

Tyrosine Hydroxylase 및 Tryptophan Hydroxylase와 정신분열병의 관련연구

도규영,방승규,백인호 大韓神經精神醫學會 2000 신경정신의학 Vol.39 No.3

본 연구에서는 세로토닌과 도파민의 속도제한효소인 tryptophan hydroxylase(TPH) 유전자의 A218C 다형성 및 tyrosine hydroxylase(TH) 유전자의 intron 1 VNTR 다형성과 정신분열병 사이의 관련연구를 시행하였다. 환자군은 가톨릭대학교 강남성모병원에 입원하였던 환자 중 정신분열병으로 진단되 374명을 대상으로 하였으며 임상아형에 따랄 양성군과 음성군으로 구분하였다. 대조군은 건강한 지원자를 대상으로 하여 393명을 포함하였다. 말초혈액 단핵구에서 DNA를 분리하였으며, 두 다형성 부위를 각각 중합효소연쇄반응에 의하여 증폭한 후, TPH A 218C 다형성은 RFLP 법으로 그리고 TH intron 1 VNTR 다형성은 은염색으로 확인하였다. TPH 유전자형의 분포는 환자군과 대조군 사이에 차이가 없었으며, 양성군과 음성군 사이에도 차이가 없었다. TH 대립유전자는 Ap : (TCAT)10, Ai : (TCAT)4CAT(TCAT)5, B : (TCAT) 9 C : (TCAT) 8, D : (TCAT) 7, E : (TCAT) 6의 6가지 유형이 확인되었다. 이 대립유전자의 분포는 환자군과 대조군 사이에서 차이가 없었으나, 양성군은 음성군에 비하여 A(Ap/Ai)의 빈도가 유의하게 높은 것으로 나타났다. TPH 유전자형과 TH 대립유전자와의 상호 관계에서는 TPH유전자형이 218C/C인 경우에서만 환자군의 TH 유전자 A형 빈도가 높은 경향을 보였다. 이상의 결과는 양성 정신분열병고 TH 대립유전자 A형 사이에 유의한 관련이 있으며, TPH 유전자형에 따른 TH 유전자의 발현이 일부 정신분열병의 발생에 영향을 미칠 수 있음을 시사한다. Neurochemical investigation has played a major role in the search for the cause of schizophrenia. Among many hypotheses, dopamine hypothesis of schizophrenia prevails despite much criticism and qualification. Recently, evidences showing the atypical antipsychotics act via serotonergic mechanism suggest serotonin system as an etiologic factor for schizophrenia. We examined the possibility of the association of enzymes critical for the synthesis of serotonin(tryptophan hydroxylase, TPH) and dopamine(tyrosine hydroxylase, TH) with schizophrenia. The regions of DNA that has been know to be polymorphic were amplified using polymerase chain reaction from the peripheral blood cells of 374 biologically unrelated schizophrenic patients and 393 healthy controls. RFLP(A218C) and NVTR polymorphism(intron 1) were examined for TPH and TH, respectively. The patterns of polymorphisms and the frequencies of each allele were not significantly different between the control and the patient groups, suggesting no possible associations of the genetic polymorphisms of TPH and TH genes and schizophrenia, However, in schizophrenics, the frequency of A type allele was significantly higher in positive group than negative group. Thess findings suggest the association of positive schizophrenia with A type allele of TH gene.

Dopamine Transporter Gene의 다형성과 알코올 의존증의 관련

최윤정,방승규,김정진,도규영,백인호 大韓神經精神醫學會 1999 신경정신의학 Vol.38 No.4

연구목적 : 도파민계는 알코올 추구와 같은 알코올 의존증과 관련된 행동에 중요한 역할을 한다. Dopamine transporter(DAT1)는 신경연접부위에서 도파민 재흡수에 관여하여 도파민 활성을 종결시키는 물질로 알려져 있다. 그러므로, DAT1 유전자가 알코올 의존증에 대한 감수성에 영향을 줄 수 있다. DAT1 유전자는 다형성을 보이는 반복된 40개의 염기서열(VNTR)을 갖는다. 본 연구에서는 DAT1 유전자 다형성과 알코올 의존증 및 알코올 의존증의 임상변인과의 관련성에 대하여 알아보았다. 방 법 : 알코올 의존증 환자 94명과 정상대조군 113명을 대상으로 말초혈액을 채취하여 분리된 DNA의 DAT1 유전자를 DNA 중합효소연쇄반응으로 증폭하였다. 증폭된 산물을 15% polyacrylamide gel에서 전기영동하여 나타나는 띠의 크기에 따라 유전자형을 구분하였다. 자료의 통계적 처리는 Fisher's exact test, oneway ANOVA를 사용하였다. 결 과 : 환자군과 정상대조군의 유전자형은 모두 Hardy-Weinberg equilibrium의 예상치와 유의한 차이가 없었다. 대립유전자는 7 repeats, 9 repeats, 10 repeats, 11 repeats의 4가지 종류로 구분되었고, 유전자형은 7/7 repeats, 7/10 repeats, 9/10 repeats, 10/10 repeats, 10/11 repeats의 5가지 형태를 보였다. 환자군과 대조군간에 DAT1 유전자형 및 대립유전자 빈도는 유의한 차이가 없었고, DAT1 유전자형에 따른 음주 시작 연령, 알코올 의존증의 발병 연령 및 잠복기간에서도 차이가 없었다. 알코올 의존증의 가족력이 있는 환자군과 없는 환자군은 DAT1 유전자형의 빈도에서 차이가 있는 경향을 보였고(χ²=6.912, p=0.081), DAT1 대립유전자의 빈도에서도 유의한 차이가 있었으며(χ²=10.193, p=0.008), lambda값은 0.046였다. 결 론 : DAT1 유전자의 다형성이 알코올 의존증의 유전적 소인이 될 가능성은 적은 것으로 보이나, 알코올 의존증의 가족내 유전과는 관련있을 것으로 시사된다. Objectives : Alcoholism is known to be a heritable disease. It has been hypothesized that dopaminergic systems play an important heritable role in human behavor related to alcohol dependence, such as alcohol seeking. Therefore, genes involved in this pathway, including dopamine transporter(DAT1), which is responsible for taking released dopamine back up into presynaptic terminals and terminating dopaminergic activity, are potential candidate that may affect susceptibility to alcoholism. Analysis of a 40-base pair(bp) repeat(VNTR) in the 3' untranslated region of the DAT1 gene revealed variable number of the repeat ranging from 3 to 11 copies. Therefore, in the present study, we examined the association between alcoholism and VNTR polymorphism of DAT1. Methods : Genomic DNA analysis with polymerase chain reaction(PCR) was used to identify the presence of a VNTR polymorphism. It was carried out within a group of 94 alcoholic patients and 113 normal controls. Results : 1) There were no significant differences in allelic or genotype frequencies between the group of alcoholic patients and controls. 2) There were no significant differences in the first drinking age, onset age and latency of alcoholism according to DAT1 genotypes. 3) There was a significant difference in allelic frequencies between alcoholics with family history and those without family history. Conclusions : These results suggested that VNTR polymorphism of DAT1 is unlikely to be a factor in the genetic etiology of alcoholism, but might be related to familial transmission of alcoholism.

Retinoic Acid가 흰쥐 태자 혀조직의 분화와 Laminin 합성에 미치는 영향에 대한 연구

김혜주,정호삼,서윤경,백두진,김원규,윤지희,이방헌 대한췌담도연구회 2000 대한췌담도연구회지 Vol.5 No.4

만성 심부전환자의 혈액에서 측정한 Tumor necrosis factor-α 및 Interleukin-6의 임상적 의의

김명구,김성구,박상호,한대희,강진환,변정득,심규혁,최병조,방덕원,온영근,현민수,권영주 순천향의학연구소 2001 Journal of Soonchunhyang Medical Science Vol.7 No.2

Background and objectives: Many conditions are responsible for the pathophysiology and progressive mechanisms of congestive heart failure. More recently, it has also become evidence that another class of biologycally activated molecules generically reffered to as cytokine these are also over expressed in congestive heart failure. Therefore, the purpose of this study was to measure concentrations of tumor necrosis factor-α(TNF-α), interleukin-6(IL-6) in mild to severe symptoms of heart failure and compare their values with those found in normal control and analysed correlation relationship between cytokine level, clinical findings and hemodynamic indicies. Subjects and Methodology: Levels of TNF-α and IL-6 were measured on pulmonary artery during cardiac catheterization in heart failure patients(n=32) and normal subjects(n=8) as well as physical examination and echocardiogram. Cytokines assay were performed on plasma using commercially available ELISA(Enazyme-Linked Immunosorbent Assay) kits. Results: Although the levels of TNF-α and IL-6 tend to increase in congestive heart failure group, the cytokines level was not made significantly statistical difference between congestive heart failure group and controls. When analyzing the correlation between the levels of PCWP(pulmonary capillary wedge pressure) and cytokines(TNF-α, IL-6), respectively, there were statistically significant correlation coefficient 0.32,(p<0.05), 0.39(p<0.01). The cytokine IL-6 and pressure of pulmonary artery were significant correlation.(correlation coefficient 0.36, p<0.02) More significantly, there was correlated with TNF-α and IL-6.(correlation coefficient 0.57, p<0.001) Conclusions: There was tended toward high concentration of TNF-α & IL-6 in congestive heart failure and significant difference for PCWP between TNF-α & IL-6, thus may be correlated with development and progression in congestive heart failure.

Feasibility Study of Laparoscopy-assisted D2 Distal Gastrectomy to Treat Advanced Gastric Cancer (COACT_1001) Design and Rationale

Bang-Ul Eom,Young-Woo Kim,Jun Ho Lee,Keun Won Ryu,Byoung-Ho Nam,Young Kyu Park,Seong Yeob Ryu,Oh Jeong,Wansik Yu,Ho-Young Chung,Sang Ho Lee,Ki Young Yoon,Chang-Hwan Lee,Sang Eog Lee,Taebong Kim,Young- 대한종양외과학회 2011 대한임상종양학회 학술대회지 Vol.2011 No.9

Antifungal Activity of Magnolol and Honokiol

Bang, Kyu-Ho,Kim, Yoon-Kwan,Min, Byung-Sun,Na, Min-Kyun,Rhee, Young-Ha,Lee, Jong-Pill,Bae, Ki-Hwan The Pharmaceutical Society of Korea 2000 Archives of Pharmacal Research Vol.23 No.1

Two neolignan compound, magnolol $(5,5^{l}-diallyl-2,2^{l}-dihydroxybiphenyl, 1)$ and honokiol $(5,5^{l}-diallyl-2,4^{l}-dihydroxybiphenyl, 2)$ were isolated from the stem bark of Magnolia obovata and evaluated for antifungal activity against various human pathogenic fungi. Compound 1 and 2 showed significant inhibitory activities against Trichophyton mentagrophytes, Microsporium gypseum, Epidermophyton floccosum, Aspergillus niger, Cryptococcus neoformans, and Candida albicans with minimum inhibitory concentrations (MIC) in a range of $25-100{\mu}g/ml$. Therefore, compound 1 and 2 could be used as lead compounds for the development of novel antifungal agents.

Functional Chemical Components in Protaetia brevitarsis Larvae: Impact of Supplementary Feeds

Chul-Ho Yoon,Seung-Ho Jeon,Yeon Jo Ha,Sam Woong Kim,Woo Young Bang,Kyu Ho Bang,Sang Wan Gal,Il-Suk Kim,Young-Son Cho 한국축산식품학회 2020 한국축산식품학회지 Vol.40 No.3

The goal of this study was to evaluate the influence of various supplementary feeds on the chemical composition and production of bioactive substances in Protaetia brevitarsis larvae. The primary feed—oak-fermented sawdust—was supplemented with a variety of substances, including aloe, apple, banana, sweet persimmon (S. persimmon) and sweet pumpkin (S. pumpkin). Crude protein and fat content were the highest in the control and S. pumpkin group, respectively. Supplementary feeds increased the content of unsaturated fatty acids, except in the group receiving S. pumpkin, in which oleic acid was the most abundant (58.2%–64.5%). Free essential amino acids in larvae receiving supplementary aloe were higher compared with the control group except for Lys and His. Polyphenol and flavonoid contents and the antioxidant activities of ABTS and DPPH were higher in all treated groups compared with the control group. Although supplementary feeds led to a decreased crude protein content in the treated larvae when compared with the control group, these treatments generally improved the levels of unsaturated fatty acids and antioxidative activity. Therefore, we suggest that among the supplementary foods tested, aloe is a better resource for P. brevitarsis based on crude protein content, free amino acids and other bioactive compounds such as unsaturated fatty acids and antioxidants.

trans- Cinnamaldehyde 유도체의 항진균활성 검색

방규호(Kyu Ho Bang),조근희(Gune Hee Jo),이영하(Young Ha Rhee) 대한약학회 2001 약학회지 Vol.45 No.5

Antifungal activities of trans-cinnamaldehyde (CA) derivatives including commercial CA derivatives as well as synthesized CA derivatives against various human pathogenic fungi were investigated. Among the derivatives tested, α- chlorocinnamaldehyde, α-bromocinnamaldehyde and α-phenyl-2,4,6-heptatrienal were more potent than CA in antifungal activity α-Bromocinnamaldehyde was the most effective in inhibiting the growth of representative fungi of dermatomycosis with minimum inhibitory cocentration (MIC) of 0.61∼9.76μg/ml. In the structure-activity relationship, introduction of the chlorine and bromine group into the C-2 of CA resulted in the decrease of MIC. Derivative with more double bands exhibited the increase of antifungal activity against various pathogenic fungi.

ADSL 모뎀용 CMOS 아날로그 송수신 필터 설계

방준호(Bang Jun-Ho),이근호(Lee Cun-Ho),유영규(Yoo Young-Kyu),윤창훈(Yun Chang-Hoon) 대한전기학회 2000 대한전기학회 학술대회 논문집 Vol.2000 No.7