계피로부터 항진균물질 AF-001 의 분리 정제 및 특성

방규호,이영하,민병선 ( Kyu Ho Bang,Young Ha Rhee,Byung Sun Min ) 한국균학회 1997 韓國菌學會誌 Vol.25 No.4

Saccharomyces cerevisiae Hansen CBS5926의 고농도 배양 및 동결건조 보존

방규호,김갑진,오덕환,이영하,Bang, Kyu-Ho,Kim, Gap-Jin,Oh, Deok-Hwan,Rhee, Young-Ha 한국미생물학회 1999 미생물학회지 Vol.35 No.4

정장용 효모로 알려져 있는 Saccharomyces cerevisiae Hansen CBS5926의 생균제 제조를 목적으로 생균수 증대를 위한 배양조건과 배양 후 얻은 균체의 효과적인 동결건조 보존법에 관하여 조사하였다. 포도당을 탄소원으로 하는 회분식과 유가식 배양에 비해 에탄올을 탄소원으로 하는 유가식 배양이 장점이 많은 것으로 나타났으며, 배양 72시간 후 2.2${\times}$10^9 cfu/ml의 생균수와 38g/l의 건조 균체량을 얻을 수 있었다. 에탄올을 탄소원으로 하는 유가식 배양으로부터 얻은 균체의 동결보존을 위한 보호제의 효능을 비교한 결과, 20% 백당(w/v)과 30% 유당(w/v)에서의 생존율이 16.3%로서 비교된 다른 종류의 동결보호제보다 효과적인 것으로 나타났다. 그러나, 백당, 유당 및 탈지분유의 혼합에 의한 동해방지 보호 효과를 비교한 결과 생존율이 12.4~16.5%로서 보호제의 혼합에 따른 생존율의 상승효과는 나타나지 않았다. Production of biomass by fed-batch culture of Saccharomyces cerevisiae Hansen CBS5926, which is used to treat intestinal disorders, was investigated using ethanol as the sole carbon source. Ethanol was a better carbon source than glucose for high cell density culture of the st-rain since it could decrease the frequency of contamination while increasing the efficiency and final productivity of the fermentation process. Under optimal conditions, 38 g/ℓ of dry cell weight with $2.2{\times}10^{9}$ cfu/㎖ of maximum viable cell count was achieved after 72h cultivation. Freeze-drying of the cultured yeast cells resulted in severe reduction of viability. Of the freeze-drying protectants tested, 20% sucrose and 30% lactose were most effective for the preservation of yeast cells with a viability level of 16.3%. A combination of skim milk and lactose with 20% sucrose(w/v) exerted no synergistic influence upo the viability of the cells during cryopreservation by freeze-drying.

trans- Cinnamaldehyde 유도체의 항진균활성 검색

방규호(Kyu Ho Bang),조근희(Gune Hee Jo),이영하(Young Ha Rhee) 대한약학회 2001 약학회지 Vol.45 No.5

Antifungal activities of trans-cinnamaldehyde (CA) derivatives including commercial CA derivatives as well as synthesized CA derivatives against various human pathogenic fungi were investigated. Among the derivatives tested, α- chlorocinnamaldehyde, α-bromocinnamaldehyde and α-phenyl-2,4,6-heptatrienal were more potent than CA in antifungal activity α-Bromocinnamaldehyde was the most effective in inhibiting the growth of representative fungi of dermatomycosis with minimum inhibitory cocentration (MIC) of 0.61∼9.76μg/ml. In the structure-activity relationship, introduction of the chlorine and bromine group into the C-2 of CA resulted in the decrease of MIC. Derivative with more double bands exhibited the increase of antifungal activity against various pathogenic fungi.

동물세포배양기술을 이용한 유전자재조합의약품 산업 현황과 발전 방안

방규호(Kyu Ho Bang),김경수(Kyeong Soo Kim) 대한약학회 2021 약학회지 Vol.65 No.1

This study was undertaken to suggest a strategy for improving the recombinant pharmaceutical industry using animal cell culture technology. The status of markets and policies of domestic and global biosimilar recombinant products and the media industry were investigated. Based on this investigation, we suggest seven ideas for improving technical development and the licensing, production, and market launch phases as a strategy for improving the recombinant pharmaceutical industry using animal cell culture technology.

Cinnamaldehyde 유도체의 항진균 활성

방규호(Kyu Ho Bang),민병선(Byung Sun Min),이영하(Young Ha Rhee) 한국균학회 1998 韓國菌學會誌 Vol.26 No.4

N/A

의약품 안정성시험 및 바이오의약품 분석지원센터 구축 연구

방규호(Kyu Ho Bang) 대한약학회 2023 약학회지 Vol.67 No.3

This study was undertaken to suggest the necessity of establishing stability test and biopharmaceutical analysis support center. Feasibility study on center establishment was performed as online survey to factory manager and person in charge in 39 pharmaceutical companies in Korea. More than seventy percent of 39 companies responded that they need to establish it. Eighty percent of respondents answered that they would entrust the entire test items of stability test and 71.8% of respondents answered that they would entrust biopharmaceutical cell bank and protein analysis. The construction cost of two centers with 2 stories and 1 basement floor and 3000m2 was 6.37 billion won. The stability test center need to 173 EA of 71 types of equipment and 4.086 billion won in budget for equipment purchases. The biopharmaceutical analysis support center need to 169 EA of 77 types of equipment and about 7.2 billion won in budget for equipment purchase. Total cost for construction and equipment purchase was 17.65 billion won. The establishment of two centers with expertise and efficiency would provide the competitiveness of the pharmaceutical industry in Korea.

미세결정셀룰로오스와 호화전분을 이용한 암로디핀말레이트 정제의 수분에 대한 안정성 개선

박호석 ( Ho Seock Park ),황우신 ( Woo Sin Hwang ),방규호 ( Kyu Ho Bang ),박정숙 ( Jeong Sook Park ),조정원 ( Cheong Weon Cho ),황성주 ( Sung Joo Hwang ) 韓國藥劑學會 2008 Journal of Pharmaceutical Investigation Vol.38 No.3

난용성 항진균제 이트라코나졸의 액상제제화에 의한 생체이용율 개선

황우신,권광일,방규호,Hwang, Woo-Sin,Kwon, Kwang-Il,Bang, Kyu-Ho 대한약학회 2000 약학회지 Vol.44 No.6

This study was to develop an effective itraconazole liquid preparation which exhibits an enhanced bioavailability. The solubility of itraconazole was increased (72-fold) in itraconazole liquid preparation as compared with itraconazole powder. The dissolution rate of itraconazole was higher for itraconazole liquid preparation filled into a hard gelatin capsule with 90% release within 20 min as compared to 55% for $Sporanox^{\circledR}$capsules. The oral absorption of itraconazole liquid preparation and $Sporanox^{\circledR}$tablets were studied in the rat. The area under the concentration-time curve $(AUC_{0-24hr})$ of itraconazole liquid preparation ($90.25\;{\pm}\;8.36\;{\mu}g{\cdot}hr/ml$) increased by 6.2 times compared to that of Sporanox tablets ($14.58\;{\pm}\;1.26\;{\mu}g{\cdot}hr/ml$) after oral administration of itraconazole 15 mg/rat each. $C_{max}$ also increased to $6.87\;{\pm}\;1.15\;{\mu}g/ml$ after administration of liquid preparation $1.58\;{\pm}\;0.16\;{\mu}g/ml$ of $Sporanox^{\circledR}$tablets. These results indicate that in vivo bioavailability of itraconazole liquid preparation was significantly enhanced as compared with $Sporanox^{\circledR}$tablets.

온단세트론을 함유한 새로운 구강붕해필름 제형의 개발

이정균(Jeong-Gyun Lee),방규호(Kyu-Ho Bang),김경수(Kyeong Soo Kim) 한국산학기술학회 2021 한국산학기술학회논문지 Vol.22 No.11

본 연구의 목적은 항암요법 및 마취와 관련하여 유발된 구토의 방지를 위하여 사용되고 있는 온단세트론을 함유하는 새로운 구강붕해필름(Orally Disintegrating Film, ODF) 처방을 개발하는 것이다. 이러한 목표를 달성하기 위하여 온단세트론에 대한 HPLC 분석 방법을 확립한 후, 다양한 pH 조건에서의 용해도 시험을 진행하였으며 실험 결과 온단세트론은 낮은 pH에서 용해도가 증가하는 것을 확인하였다. 다양한 수용성 고분자를 평가하여 필름의 베이스 처방을 선정하였으며, 가소제를 추가하여 필름의 물리적 특성과 붕해속도를 고려하여 최종적으로 구강붕해필름 조성물을 선정하였다. 최종 선정된 구강붕해필름의 조성물은 온단세트론, 히프로멜로오스, 포비돈, 글리세롤, 폴리에틸린글리콜 1500, 폴리소르베이트 20, 수크랄로즈, 포도향분말 및 적색산화철의 질량비가 4/20/5/3/3/4/0.3/0.4/0.3인 필름 처방으로 가장 우수한 내절강도 및 가장 빠른 붕해시간을 기록하였다. 시판 구강붕해정인 온세란정<SUP>®</SUP>과의 비교용출시험 결과 pH 1.2 완충액 및 pH 6.8 완충액에서 15분 시점까지 유의적으로 빠른 용출속도를 보여서, 구강붕해필름으로 사용 시 더 빠른 흡수를 기대할 수 있을 것이다. 따라서, 본 연구를 통해 얻어진 온단세트론 구강붕해필름 조성물은 시판 제품 보다 복약순응도가 우수하고, 용출속도가 빨라 시판 구강붕해정을 대신할 수 있는 우수한 의약품으로 활용될 수 있을 것이다. The purpose of this study was to develop a novel orally disintegrating film (ODF) formulation containing ondansetron as an antiemetic for cancer treatment-induced and anesthesia-related nausea and vomiting. For this purpose, an HPLC analytical method of ondansetron was established, and then an aqueous solubility test with various pH buffers was performed. Ondansetron showed an increased solubility as the pH of the buffer decreased. After the hydrophilic polymers were assessed for the formation of a base film, various plasticizers were evaluated for their effect on physical properties and in vitro/in vivo disintegrating time. Among the tested ondansetron ODF formulations, the one composed of ondansetron, hypromellose (HPMC), povidone, glycerol, polyethylenegylcol 1500, polysorbate 20, sucralose, grape flavor, and ferric oxide red in the weight ratio of 4/20/5/3/3/4/0.3/0.4/0.3 showed the highest folding endurance value and the fastest disintegrating time. As a result of a comparative dissolution test (pH 1.2 and pH 6.8 buffer) with commercial ODT Onseran<SUP>®</SUP>, the final selected ODF formulation showed significantly faster dissolution results until 15 minutes than commercial ODT. Thus, this novel ondansetron ODF formulation could be an effective alternative to the commercially available ODT with a faster dissolution rate and increased compliance.

Candida와 Penicillium속 진균에 대한 천연물의 항진균 효과 검색

민병선(Byung Sun Min),방규호(Kyu Ho Bang),이준성(Jun Sung Lee),배기환(Ki Hwan Bae) 대한약학회 1996 약학회지 Vol.40 No.5

For the research of antifungal active constituents from natural products, 226 plants were extracted with ether and methanol, separately, and screened antifungal activity against Candida albicans and Penicillium avellaneum cells. The results demonstrated that 30 samlpes showed antifungal activity in ether or methanol extracts and 17 samples in ether extracts and 20 samples in methanol extracts against C. albicans. Against P. avellaneum, 19 samlpes showed antifungal activity in ether or methanol extracts and 17 samples in ether extracts and 11 samples in methanol extracts, respectively. The antifungal activity of natural products against C. albicans were showed more sensitive than P. avellaneum, and the polarity of the solvent was not specific in antifungal activity.