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단치소요산이 구속 스트레스를 가한 흰쥐의 뇌내 catecholamine 함량변화에 미치는 영향
황귀서,이기선,박종형 한국환경독성학회 1998 환경독성보건학회지 Vol.13 No.2
To elucidate the preventive effect of Danchisoyosan (DS) on stress, we investigated the physiological change of rats which were applied immobilization stress. For immobilization stress, rats were placed in restrainer for 12 hours a day for 3 days. During application of stress, body weight of rats was measured. After sacrifice, 8 organs were taken for measurement of organ weight. Brain was sectioned into 4 parts that are Frontal Cortex, Corpus Striatum, Hypothalamus and Hippocampus. Each part was homogenated and its catecholamine and serotonin contents were measured with HPLC. In our study, stress mainly induced increase of concentration of neurotransmitters in brain without other significant physical change of rats. DS inhibited stress induced changes of neurotransmitter content in brain.
이종흔,정동균,김중수,이효재,김각균,오귀옥,정태영 대한구강생물학회 1979 International Journal of Oral Biology Vol.3 No.1
Mineral analyses of water samples from several areas of the Mankyung River and the Dongjin River regions in Chonrabukdo where dental fluorosis was observed, which had been conducted between June in 1978 and January in 1979, have shown the following results. In general, the mean calcium concentrations were high in the areas where the mean concentrations of phosphorus and fluoride were low. And in the same area where dental fluorosis was particularly observed, seasonally a few minerals were variable─namely, calcium, phosphorus, and fluoride. Those samples of high fluoride concentration have higher phosphorus concentration, but calcium concentrations were the opposite phenomenon. Particularly, fluoride concentrations were remarkably greater during the cold season(January) than during the rainy season(July).
Agrobacterium tumefaciens를 이용한 amorpha-4,11-dience 합성 유전자의 상추로의 도입
정귀미,송명종,백남인,박영두 경희대학교식량자원개발연구소 2005 硏究論文集 Vol.24 No.-
Amorpha-4,11-diene synthase(ADS) 유전자를 상추에 도입하기 위해 ADS유전자를 pILTAB357에 삽입하여 binary vector pADS를 제작하였다. 상추 형질전환은 자엽 절편체를 pADS 벡터를 운반하는 A. tumefaciens LBA4404에 접종시키고 MS배지에 200 mg·L 세파탁심과 50 mg·L 가나마이신이 첨가된 선발 재분화배지에서 선발하였다. 선발된 신초는 뿌리 유기 배지에서 뿌리를 유기한 후 뿌리가 발생한 신초는 토양으로 이식하여 재배하였다. 형질전환상추에 T-DNA가 전이되었는지를 확인하기 위해 genomic DNA를 이용하여 PCR과 Southern 혼성화 분석을 실시하였다. PCR 분석 결과 9개의 재분화 개체로부터 기대했던 1.6 kb의 산물을 확인할 수 있었으며 Southern 혼성화 분석 역시 각 재분화 개체에 ADS 유전자가 1개 내지 3개의 copy수로 전이된 것을 확인할 수 있었다. 형질전환 상추내의 artemisinin 함량을 GC와 GC/MS 방법으로 분석하였다. Artemisinin 표준물은 retention time이 10분 7초로 나타났으며 이 peak가 artemisinin인지 확인하기 위하여 GC/MS를 측정한 결과 m/z 282가 정확하게 나타나 artemisinin으로 확인되었다. 한편 형질전환 상추와 일반 대조구 상추의 시료도 GC chromatogram에서 artemisinin의 retention time인 10분 7초 부근에서 여러 peak이 겹쳐서 나타났으나 각 peak에 대하여 GC/MS를 측정한 결과 m/z 282을 나타내는 peak는 관측되지 않아 형질전환 상추와 일반 상추에는 artemisinin이 함유되어 있지 않은 것으로 확인되었다. To introduce amorpha-4,11-diene synthase (ADS) gene into lettuce plant, binary vector pADS was constructed by the insertion of an ADS gene to pILTAB357. Cotyledon explants were infected by A. tumefaciens LBA4404 carrying pADS vector to transform lettuce plant with ADS gene. Transformed shoots were selected on selective regeneration medium containing MS salts, 200 mg·L cefotaxim, and 50 mg·L kanamycin. The selected shoots were transferred to rooting medium, and regenerated shoots were transplanted to pots after roots appeared. PCR and Southern hybridization analysis of genomic DNA were performed to confirm expected T-DNA fragments in the transgenic lettuce plants. PCR analysis revealed the presence of the expected 1.6 kb product from the nine regenerants. Southern hybridization analysis also showed that ADS gene was transferred into transgenic lettuce plants with 1~3 copies. Artemisinin in lettuce was identified by GC and GC/MS analysis. Standard artemisinin was found at 10 min 7 sec in the retention time by GC analysis and confirmed m/z 282 by GC/MS. There were several peaks at retention time of both non-transformed and transformed lettuce plants. However, GC/MS analysis showed that peak with m/z 282 was not found in transgenic lettuce plants.
신경회로망을 이용한 NARMAX 모델의 교류 서어보 모터의 적응 제어와 식별에 관한 연구
박양수,이종헌,최부귀 東亞大學校 附設 情報通信硏究所 1996 情報通信硏究所論文誌 Vol.4 No.1
This paper proposes the use of Artificial Neural Networks(ANNs) to identify and control the NARMAX model of the AC serve motor. Two systems are presented : a system to adaptively control the stator currnets via identification of the electrical dynamics, and a system to adaptively control the rotor speed via identification of the motor and currnet-fed system dynamics. Various advantages of these control schemes over conventional schemes are cited and the combined speed and current control scheme is compared with the standard vector control scheme.
丁東均,李種昕,李孝宰,金各均,吳貴玉,鄭泰英 대한구강생물학회 1979 International Journal of Oral Biology Vol.3 No.1
The urinary fluoride concentration and the degree of dental fluorosis of all the schoolboys attending Chimoon primary school in Baikku-myun, Kimjae-kun and the fluoride concentration of drinking water of those areas were investigated in an effort to elucidate the relation between them. The urinary fluoride concentration and the fluoride concentration of driking water were measured by fluoride ion electrode, and dental fluorosis was surveyed and analysed by Dean and McKay's and Horowitz's method. 1) The mean urinary fluoride concentration and the mean fluorosis index of all the schoolboys were 1.89ppm (0.12-12.2ppm) and 0.79 (0-1.99). The mean fluoride concentration of drinking water of those areas was 0.61ppm (0.027-2.66ppm). 2) The mean urinary fluoride concentration and the mean fluorosis index of the schoolboys residing in Yookang-ri 5-ku, Baikku-myun (Dongja-po) were the highest (3.23ppm, 1.99) among the statistics for the schoolboys residing in different areas. Incidentally the fluoride concentration of drinking water for that area was also the highest (1.99ppm). 3) The urinary fluoride concentration was two to ten times as high as the fluoride concentration of drinking water regardless of the resident area of schoolboys. 4) There was no significant seasonal change in fluoride concentration of drinking water collected from the well in the resident area of schoolboys. 5) There showed a relatively parallel relationship between the urinary fluoride concentration and the fluorosis index of schoolboys. 6) The urinary fluoride concentration and the fluorosis index of schoolboys tend to increase with age increasing.
정동균,이종흔,김각균,이공훈,오귀옥,정태영 대한구강생물학회 1980 International Journal of Oral Biology Vol.4 No.1
Those studies were performed to elucidate the effects of several dietary spices on dental caries and to determine which component of spices induced the change of incidence of dental caries in Sprague-Dawley rats which were supplied from SNU animal house. For this study, 132 rats (rats weighing 90-140 gm) were chosen at random with the same number of both sexes and divided into eleven experimental groups which contained the differential components of drinking water and diets for control and experimental group. Control group was fed standard cariogenic diet and tap water. In experimental groups, rats were fed standard cariogenic diet supplemented with capsicum (10%), ash capsicum (1, 10%), garlic (5%), common salt (3%), and analytical salt (3%) for 80 days. Drinking water was tap water containing water extract of capsicum (1, 10%), water extract of garlic (1.1%), and fluoride (50ppm). The results were as follows. 1. 54.6 in caries score was recorded in control group. 2. Rats which drank water containing 50ppm of fluoride showed 18.4% decrease in caries score. 3. Capsicum-fed rats showed 10.7% (capsicum 10%) increase in caries score and rats which drank water containing water extract of capsicum (1, 10%) showed 14.1% (w.e. capsicum 1%) and 0.16% (w.e. capsicum 10%) increase in caries score. But ash capsicum-fed rats showed 18.7% (ash capsicum 1%) and 7.3% (ash capsicum 10%) decrease in caries score. 4. Garlic-fed rats showed 22.1% (garlic 5%) decrease in caries score. But rats which drank water containing 1.1% of water extract of garlic showed 24.1% increase in caries score. 5. 58.4% and 68.0% increase in caries score were recorded in rats which were fed diet containing common salt (3%) and analytical NaCl (3%), respectively.
정동균,정태영,이종흔,김중수,오귀옥,이효재 대한구강생물학회 1980 International Journal of Oral Biology Vol.4 No.1
This experiment was conducted to elucidate the relation between the urinary fluoride concentration and the fluorosis index of residents in Mankyung River valley, especially in 3 areas (Yookang-ri, Koje·Yoochun, Shinbok·Yongkang) with high fluoride concentration in drinking water. The urinary fluoride concentration was measured by fluoride ion electrode, and dental fluorosis was surveyed and analysed by Dean and McKay's and Horowitz's method. 1. There was no reletionship between the urinary fluoride concentration and age. 2. 11-20 years age groups has revealed the highest fluofosis index. In this group : 3. There showed a parallel relationship between the fluorosis index and the urinary fluoride concentration. 4. DMF rates of residents in Yookang-ri, Koje and Shinbok are 59.2, 48.4 and 47.1%, respectively. 5. There showed a parallel relationship between the years of residence within 8 years after birth and the fluorosis index.
간편 퍼지와 디지털 PI+D를 이용한 제어 시스템의 설계
박윤명,권태익,박종오,임영도,최부귀 동아대학교 정보기술연구소 2000 情報通信硏究所論文誌 Vol.8 No.1
본 논문에서는 퍼지 디지털 PI+D제어기를 이용하여 외란, 잡음, 플랜트 변화 등에 강한 제어시스템을 설계한다. 퍼지제어는 간단한 4개의 규칙과 소속함수를 유도하여, 간편 퍼지를 이용한 디지털 PI+D 제어기를 설계하였으며 또한 플랜트는 2차 선형 플랜트를 적용하였다. 본 논문에서 제안한 제어 시스템과 디지털 PID 제어, 디지털 PI+D 제어 시스템을 시뮬레이션을 통해 각 시스템의 특성을 비교 하였다.
안기형,박현규,최부귀,이종헌 東亞大學校 附設 情報通信硏究所 1996 情報通信硏究所論文誌 Vol.4 No.1
This paper consist of new secure communication system from using synchronization methode of chaotic signal which Pecora and Carroll have shown, and experimentally proved the validity of this communication system. This communication system transmits the informational signals via the modulation of chaotic signals, and the receiver recovers the original informational signals from modulate chaotic signals by synchronization of chaotic signals driven together Chua circuit was used in generating chaotic signals and nonlinear resistor of this Chua circuit was confirmed by using two OP amps and six resistors.
정태영,정동균,이효재,김각균,오귀옥,이종흔 대한구강생물학회 1979 International Journal of Oral Biology Vol.3 No.1
Insoluble extracellular polysaccharides synthesized by Streptococcus mutans type d strain B-13 were analysed in an effort to elucidate their chemical composition. Dextransucrase produced in supernatant was also studied for its activity. 1. The microorganism produced 2.10g of total insoluble fraction which contained 1.59g of insoluble polysaccharide and 0.41g of bacterial mass per 800ml of culture medium. 2. Insoluble fraction contained 26.1㎍/mg of nitrogen and 746.4㎍/mg of insoluble extracellular polysaccharides. Ketohexose content of polysaccharides was 7.12%. 3. Streptococcus mutans strain B-13 was capable of producing 4.10g insoluble extracellular polysaccharide per 1.0g of bacterial mass. 4. Dextransucrase liberated 5.11mg of hexose per 1ml of culture supernatant.