Arachidonic acid 대사산물이 배양골조직의 골흡수에 미치는 영향에 관한 연구

오귀옥,김세원 대한구강생물학회 1989 International Journal of Oral Biology Vol.13 No.2

This experiment was performed to study the arachidonic acid metabolites (prostaglandin E_2, leukotriene B_4 and HETE) and indomethacin of the bone resorption and the effect of indomethacin on the PGE_2-stimulated bone resorption in the organ culture. Ulnae and radii or calvaria were removed from 19-day fetal rats prelabelled by subcutaneous injection of 200 μCi ^45CaCl_2 into mother rats at the 17th day of gestation. The control groups were cultured in fresh BGJb media, while experimental groups were cultured in media containing PGE_2 (10^-6M), indomethacin (10^-5M), leukotriene B_4 (0.1pM, 1pM, 10pM, 100pM and 1nM) or HETE (0.1pM, 1pM, 10pM, 100pM and 1nM) for 48 or 72 hours. Radioactivities of ^45Ca released into media and remained in bone after culture were respectively determined. The effects of each drugs were observed by the ratios of %-release of ^45Ca between control and experimental group. The observed results were as follows. 1. PGE_2, at concentration of 10^-6M, increased ^45Ca release significantly after 24 and 48 hours of culture. 2. Indomethacin, at concentration of 10^-5M, decreased ^45Ca release after 24 and 48 hours of culture, but there was no significant difference. 3. LT and HETE, at all concentrations, had no effect on the bone resorption after 72 hours of culture. 4. Indomethacin, at concentration of 10^-5M, had no effect on the PGE_2-stimulated bone resorption after 72 hours of culture.

Streptococcus mutans에서의 세포외다당류 합성효소의 활성에 미치는 수종약물의 영향

오귀옥,정동균 대한구강생물학회 1980 International Journal of Oral Biology Vol.4 No.1

Streptococcus mutans synthesize insoluble glucans from sucrose by the cell-associated and extracellular glucosyltransferase and these glucans gives the characteristic adhesive nature to the plaque which contains S. mutans. As the insoluble glucans in dental plaque apparently play a cardinal role in the cariogenic otential of S. mutans inhibition of glucan synthesis could be a contributory factor in the prevention of dental plaque, and ultinatively dental caries. The water extract of capsicum and several antibacterial compounds, including surface-active agents previously shown to be plaque inhibiting, were tested for their effect on glucan synthesis by an extracellular glucosyltransferase preparation from S. mutans B-13. The results were as follows. 1. Crude enzyme preparation was found to contain 0.23 unit/ml of enzyme activity (1 unit transforms 1 μmole of sucrose in 1 minute) as measured turbidimetrically and also by measuring the incorporation into polysaccharide of radioactivity from radioactive sucrose. 2. Surface-active agents (chlorhexidine, cetylpyridinium, sodium lauryl sulfate) were found to be potent inhibitors of enzyme activity. Both electrostatic and hydrophobic interactions appear to be occurring between the cation of test agents and the glucosyltransferases, with hydrophobic interaction being more important for enzyme inhibition. 3. Iodine preparation (providone iodine) was also found to inhibit enzyme activity perhaps through the iodination mechanism. 4. Some unknown agents in water extract of capsicum could stimulate the enzyme activity in the presence of strong inhibitory effect of glucose.

손상자극하에서 Diazepam이 흰쥐 뇌부위의 Dopamine 함량에 미치는 영향

오귀옥,김명수 대한구강생물학회 1987 International Journal of Oral Biology Vol.11 No.1

This experiment was carried out to observe the effects of diazepam on dopaminergic neuronal activties of rat brain regions, for which rats were sacrificed and brains were dissected by the method of Glowinski and Iversen(1966) following administration of diazepam to non-traumatized or traumatized (mandibular angle fracture) rats. Regional amines were extracted and detected by HPLC-EC method. The results were as follows. 1. There were some decreases in dopamine contents of frontal cortex (15.1%) and cerebellum (29.1%) of traumatized rats without statistical significance, but not of hypothalamus and medulla and pons. 2. By traumatic stress norepinephrine contents were significantly in the order of hypothalamus (53.4%, p<0.01 ), frontal cortex (29.2%, p<0.05), medulla and pons(22.3%, p<0.01), and cerebellum(15.1%,NS). 3. All the brain regions except the frontal cortex under study showed dose-dependent increase in dopamine contents by administration of diaxepam with significance. 4. Diazepam-induced increase in dopamine contents were more conspicuoous in traumatized rats than non-traumatized rats; in traumatized gorup, diazepam had produced significant change in dopamine contents of hypothalamus, medulla and pons, and cerebellum, however, in traumatized group, only hypothalamus showed statistically significant change.

Lidocaine이 흰쥐 뇌부위의 Monoamine교체율에 미치는 영향에 관한 HPLC 전기화학분석적 연구 : BY REVERSE-PHASE ION-PAIR HIGH PERFORMANCE LIQUID CHROMATOGRAPHY WITH ELECTROCHEMICAL DETECTION METHOD

오귀옥,정동균 대한구강생물학회 1984 International Journal of Oral Biology Vol.8 No.1

This experiment was performed to elucidate the mechanism of lidocaine-induced systemic convulsion by studying the effect of lidocaine on noradrenaline (NA) turnover in the rat brain regions. Lidocaine HCI (50 mg/kg), α-methyl-p-tyrosine (250mg/kg: AMPT), AMPT + lidocaine HCI(25 mg/kg), or AMPT + lidocaine HCI (50 mg/kg) were administered, via I.P., to the groups of albino rats which were sacrificed by cervical dislocation 0, 2, 4, and 6 hours later to determine the NA content in 4 areas in the brain, namely, hippocampus, frontal cortex, striatum, and hypothalamus, The brains were dissected by the method of Glowinski and Iversen(1966), and the NA contents were determined by reverse phase high performance liquid chromatography-electrochemical detection method after homogenization and amine extraction by aluminum oxide adsorption. The results were as follows: 1. Calibration curves for noradrenaline and dopamine obtained from HPLC-EC analysis were accurately linear in the range from 100 pg to 100 ng. 2. Disappearance of noradrenaline after synthesis inhibition (AMPT treatmint) showed linearity on the semilogarithmic scale in hippocampus. frontal cortex and striatum, but not in hypothalamus. It was also shown that hippocampus had the most rapid amine turnover rate. 3. Lidocaine-treated groups showed higher concentration of noradrenaline in hippocampus at 4 hour (50 mg/kg, p < 0.1) and 6 hour (25 mg/kg, P < 0.1; 50 mg/kg, p <0.05), and hypothalamus at 4 hours (50 mg/kg, p < 0.1) after drug injection, when compared with respective AMPT-treated groups. 4. By the injection of lidocaine 25, 50 mg/kg, turnover rates (2 to 4 hour) of noradrenaline were decreased by 33% (p < 0.1), 57% (p<0.05) in hippocampus respectively, when compared with AMPT treatment.

Putative proinflammatory cytokine유전자의 발현양상과 수용체 분자의 cloing

오귀옥,송요한,서영석,이동환,문대희,김형섭,Oh, Kwi-Ok,Song, Yo-Han,Seo, Young-Seok,Lee, Dong-Whan,Moon, Dae-Hee,Kim, Hyung-Seop 대한치주과학회 1994 Journal of Periodontal & Implant Science Vol.24 No.3

Cytokines expressed specifically in leukocytes subsets and in activated cells, which are involved in chemotaxis and activation of leukocytes, are recently defined as chemokines. Macrophage inflammatory $protein-1{\alpha}(MIP-1{\alpha})$ and $MIP-1{\beta}$ are members of C-C chemokine subfamily which produces wide immunomodulatory, proinflammatory, and hematopoietic modulatory actions. We have studied their gene expression by using Northern blot analysis in various blood cells such as cytolytic T lymphocyte(CTL), helper T lymphocyte(HTL), macrophage, and B lymphocyte. Resting CTL line CTLL-R8 expressed $MIP-1{\alpha}$ mRNA which was downregulated by ConA stimulation. Both of resting and ConA stimulated HTL line Hut78 and Jurkat did not express $MIP-1{\alpha}$ mRNA. There was detectable $MIP-1{\alpha}$ transcript in HTL hybridoma 2B4.11 which was a little upstimulated by ConA stimulation. B cell line 230, and macrophage cell line RAW264.7 and WR19M.1 showed distinct $MIP-1{\alpha}$ message which were induced after LPS stimulation. Expression pattern of $MIP-1{\beta}$ in all cell lines or cell were almost identical to that of $MIP-1{\alpha}$. Also strategies employed to identify and characterize the biological functions was preceded by receptor cloning to trace the shorcut to the final goal of cytokine research. For the cloning of $MIP-1{\alpha}$ receptor(R), we used synthetic oligonucleotides of transmembrane(T) conserved sequences of already cloned human(h) IL-8-R, and performed reverse transcription-polymerase chain reaction(RT-PCR) amplification using murine(m) macrophage cell line mRNA. Among 5RT-PCR products, we isolated a homologous cDNA with hIL-8-R which were shown to be putative mIL-8-R cDNA.

국소마취제의 중추적 독성과 뇌부위별 dopamine 성 신경활동에 관한 연구

오귀옥,박병건,조남표,송요한,박수정 全北大學校 齒醫學硏究所 1987 전북치대논문집 Vol.5 No.1

Administration of convulsive dose of local anesthetics is believed to change the activity of central aminergic neuron in laboratory animals. In the present study. subconvulsive dose of lidocaine(25, 50 ㎎/㎏ i.p) was injected into rats after synthesis inhibition of catecholamine by α-methyltyrosine administration (250 ㎎/㎏ i.p), which were killed after 0, 2, 4 hours. Four brain parts were analyzed for dopamine by HPLC-electrochemical detection. Lidocaine induced the significant increase of dopamine concentration in striaturn (2, 4hr) and hypothalamus (4 hr), but dose-dependent effect was observed only in the striatum. Dopamine turnover rate was also decreased in the striatum and hypothalamus with statistical significance. However, hippocampus and frontal cortex showed no significant: change of dopamine concentration and turnover rate.

Expression of Recombinant Macrophage Inflammatory Proteins

Oh, Kwi-Ok,Kim, Kack-Kyun 대한구강생물학회 1990 International Journal of Oral Biology Vol.14 No.2

T 임파구로부터 추출한 cDNA library에서 얻은 몇 가지 미확인 분자 중 L2G25B와 L2G25C는 그 추측 아미노산 배열이 대식세포 염증 단백질 MIP-1α 및 -B(macrophage inflammatory protein-1α, -1β)와 같음이 알려졌다. MIP-1α^1와 -1β는 분자량이 약 5kDa이며 아미노산 배열이 67%까지 일치하고, 골수내에서 조혈을 조절하는 효과를 보이는 것으로 밝혀졌다. 특히 MIP-1α는 초기 전구세포(progenitor cell)에 대하여 억제 효과를 보이며 MIP-1β는 그러한 MIP-1α의 작용을 상쇄하는 것으로 알려져 그 기전을 밝히는 일은 매우 의미있는 일로 생각된다. 따라서 저자는 L2G25B 및 C를 각각 bovine papilloma viral vector 및 baculoviral vector system을 이용, C127 조섬유세포 및 sf21 곤충세포에서 다량 발현, 순수분리하였다.

Arachidonic acid 대사산물의 골흡수능

김형섭,오귀옥,장기완 대한구강생물학회 1991 International Journal of Oral Biology Vol.15 No.2

This experiment was performed to study the effect of the arachidonic acid metabolites(PGE_2, LTB_4, HETEs) on the bone resorption in the organ culture. The observed results were as follows: 1. PGE_2, at concentration of 10^-6M, increased ^45Ca release significantly after 72 hours of culture. 2. LTB_4 or HETEs, at all concentrations, had no effect on the bone resorption after 72 hours of culture. 3. LTB_4 or HETEs did not change the bone resorbing activity of PGE_2 bone resorption after 72 hours of culture.

염증세포의 생물학적 활성에 대한 Granulocyte Macrophage - Colony Stimulating Factor의 효과

문대희,오귀옥,장기완 ( Dae Hee Moon,Kwi Ok Oh,Kee Wan Chang ) 대한예방치과·구강보건학회 1996 大韓口腔保健學會誌 Vol.20 No.2

면역세포 활성에 대한 Substance P의 영향

김형섭,오귀옥,임종득,Kim, Hyung-Seop,Oh, Kwi-Ok,Lim, Chong-Deuk 대한치주과학회 1996 Journal of Periodontal & Implant Science Vol.26 No.2

The neuropeptide substance P(SP) has been recognized to modulate immune systems, with close proximity between peptidergic sensory nerve endings and immune cells. These include the macrophage and neutrophil activation, IL-2 production in T cell, augmentation of Ig synthesis, mast cell degranulation, $PGE_2$ and collagenase secretion in synoviocytes. In this study I examined SP-induced various biological activities such as antimicrobial action, cytokine production, and mast cell degranulation in the presence or absence of other inflammatory cell activators. Antimicrobial studies showed that undifferentiated HL-60 cells were not affected by SP. However, SP significantly enhanced antimicrobial action of TPA-treated or dbcAMP-treated HL-60 cells which had been differentiated into PMN or macrophage/monocyte. I could not find synergistic relationship between SP and LPS in parallel experiments of the above. SP did not induce IL-l production from murine macrophage cell line RAW264.7 whether costimulated with LPS or not. Mast cell degranulation was occured only when stimulated with high dose ($10^{-5}M$) of SP and the degree of this activation was slightly reduced by simultaneous application of $MIP-1{\alpha}$. In addition, CGRP which is known to be a common coexisting neuropeptide with SP within specific fibers did not augment the function of SP on mast cell degranulation. These results suggest that immunoregulatory activities of SP could be mediated through direct upregulation of various functions of immune cells and also upregulation of responsiveness of immune cells to other immune activators.