단감에서 Deltamethrin의 안전사용기준 설정을 위한 잔류량 평가

심재한,이강봉,서용택,양광록 全南大學校 農漁村開發硏究所 1990 農業科學技術硏究 Vol.25 No.-

Deltamethrin의 단감 재배기간중 살포횟수 및 처리시기에 따른 잔류수준을 시험하여 다음과 같은 결과를 얻었다. 1. Deltamethrin의 잔류분석 회수율은 단감시료에 대해 0.1ppm과 1.0ppm에서 각각 평균 90.20%, 90.73%를 보였고 검출한계는 0.01ppm이었다. 2. Deltamethrin의 단감에서의 잔류는 Mills 등의 방법으로 분석한 결과 수확 3일전 6회 처리구에서만 0.16ppm으로 잔류 허용기준을 초과하였고 이로 미루어 deltamethrin의 단감중 안전사용은 수확 3일전 5회 이내의 사용으로 추천할 수 있었다. The effects of application number on the persistence of deltamethrin in sweet persimmons was analyzed. Deltamethrin EC(1%) was sprayed on the sweet persimmon trees at regular intervals (400 L/10a, 1,000 times diluted deltamethrin EC was sprayed on 60th, 45th, 30th, 15th, 7th, 3rd days before harvest). The procedure of residue analysis was based on the multiresidue analytical method using a polarized acetonitrile solution as the extracting solvent. Residue of deltamethrin were determined by alternate eluate (acetonitrile, hexane, dichloromethane 1.5 : 48.5 : 50 v/v) from the Florisil column to gas chromatographic analysis (ECD). Residue level of deltamethrin in sweet persimmons were proved to 0.16 ppm when the deltamethrin EC (1%) was sprayed 3 days before harvest. The recoveries were above 90% in spiked sweet persimmon samples, regardless of concentration.

위암종과 Epstein-Barr 바이러스와의 연관성에 대한 연구

심광용,김호영,김효열,백순구,권상옥,조미연,이창훈,이종인 건국대학교 의과학연구소 2004 건국의과학학술지 Vol.14 No.-

Objectives: Epstein-Barr virus (EBV) has been known to be linked to a spectrum of neoplastic conditions, including nasopharyngeal carcinoma, Burkitt's lymphoma, peripheral T-cell lymphoma and Hodgkin's disease. This study aims to investigate the association of EBV with gastric carcinoma in Korea. Methods: Fifty-three cases of gastric adenocarcinoma were studied for evidence of EBV infection by EBV-encoded small RNA (EBER) in situ hybridization (ISH) on the paraffin sections and amplifying the EBV genome encoding envelop glycoprotein (gp220) with polymerase chain reaction (PCR) in fresh gastric cancer specimens. Results: EBER was detected in 7 (13.2%) of 53 gastric carcinomas and all cases were male. In 6 (85.7%) of 7 EBER-positive cases, the tumors were located in the upper and middle part of the stomach. EBER ISH study showed strong positivity in all the tumor cells, but negativity in surrouning lymphocytes, stromal cells and normal gastric mucosa. DNA PCR was positive in 23 (43.4%) of 53 gastric carcinomas, including all of 7 EBER-positive cases. Conclusion: We could observe some association of EBV with gastric carcinoma, and our findings about the charateristics of EBV-positive gastric cancers involving sex, site and histological type are similar to those in Japan. However, EBV-positive rate of gastric cancer is higher than in Japan and lower than in Western countries. Further studies to elucidate oncogenic mechanism of EBV in gastric cancer should be performed.

Chitosan 濾過 System을 利用한 새로운 中水道 工程의 開發과 溫泉水 淨化에 관한 硏究

김용범,백광욱,안병제,조영주,심재용 서울産業大學校 1994 논문집 Vol.39 No.1

각종 금속이나 중금속을 비롯하여 각종 오염물(SS, COD, BOD, CI 등)을 강력히 흡착제거하는 성능을 가진 chitosan(Cs)응집제와 여과사(SF) 및 활성탄 column(ACC)을 병용하여 hotel잡수와 온천사용수등의 정화실험을 행하여 생물학적 처리과정을 생략할 수 있는 사용수의 재활용 process(Fig. 1)를 개발하였다. 이 process에서 hotel 잡수의 경우 20ppm의 chitosan 투입으로 1차응집 처리하여 약 60%이상의 오염물을 제거한 후 여과사와 활성탄 column을 이용하여 최종적으로는 80~99%의 오염물을 제거할 수 있었다. 또한 온천수의 경우 20ppm의 chitosan 투입으로 1차응집 처리하여 약 50%이상의 COD와 BOD의 제거율을 달성할 수 있었다. 따라서 이러한 process를 산업현장에 적용할 경우 종전의 생물학적 공정을 생략하거나 소규모화하여 보다 간편하며 처리공간이나 경비등을 절감하면서도 사용수를 방류수 또는 재활용수 기준이내로 처리할 수 있음을 확인하였다. 본 연구는 수자원 보호나 수자원 재활용면에서 크게 기여 되리라 믿는다. A simplifid and economic process for recycling the used water discharged from a large scale residence(e. g. hotel and apartment etc.) was developed. The used water was purified through adsorption and flocculation of contaminants by chitosan at the first stage where the average removal rates of COD and BOD of the used water were found more than 60%. The previously treated water through flocculation by chitosan was subjected to filtration systems of a sand filter and an activated carbon column, and the average removal rates of COD and BOD were found more than 80%. The experimental results suggest that the biological process which is very popular in the treatment of the used water for the removal of COD and BOD can be omitted and expected to reduce a spacial and running expenditure. On the other hand, testing the used water discharged from hot springs with chitosan proves a possibility of development of a simple and economical process for the treatment of the used water to the criteria of recycled water quality without any addition of more complicated equipments and facilities.

Al-8.7Zn-2.8Mg-2.6Cu+0.1wt.%Sc Al 합금 압출재의 시효 거동 및 기계적 성질

김광삼,심성용,임수근,이상용 慶尙大學校 經營行政大學院 2004 工學硏究院論文集 Vol.20 No.-

7000계 알루미늄 합금은 시효경화에 의해 기계적 성질이 크게 향상됨으로 항공기 등 수송기기의 구조재료로서 이용되고 있으며, 합금이 개발된 이후 지속적인 성능향상을 위한 연구가 진행중이다. Al 합금에 있어 Sc 첨가는 Al_(3)Sc 상의 생성에 의한 결정립 미세화 및 분산 강화에 의해 효과적으로 강도를 향상 시킬 수 있다. 따라서 본 연구에서는 Al-8.7Zn-2.8Mg-2.6Cu+0.1wt.%Sc 합금 압출제를 이용하여 시효 열처리를 실시하여 시효 거동과 기계적 성질을 조사하였으며, 열처리에 따른 기계적 성질을 조사하였다. 압출재의 미세 조직 관찰 결과 Al_(3)Sc상이 기지 전반에 압출 방향으로 분포되어 있음을 알 수 있었고, 시효 거동 관찰 결과 최대경도 지점이 2400분에서 나타났으며, 시효 경화성 또한 기존의 Al7075 합금 보다 우수하였으며, 강도 증가에 있어서도 T6 처리한 경우 인장강도 657MPa로 나타났으며, 연신율의 경우 5%이었다.

여성들의 자기효능감과 건강증진행위에 관한 연구

정숙희,하안례,심선녀,이용환,김광혁 고신대학교보건과학연구소 2003 보건과학연구소보 Vol.13 No.-

The results were as follows : The higher the degree of self-efficacy feeling, one of the recognition factor was the more did they do health-promoting activities (γ=0.48, p=0.00). The conducting degree of health-promotion activities resulted in 3.79 points in the perfect score of 6point, which was a quiet high result. The subordinate areas under the health-promoting activity were adjustment of personal relationshiP (4.42), nutrition (3.93), stress control (3.87), self-realization (3.64), alcoholic drink/smoking (3.38), and responsibility for health. As for the differences of self-efficacy feeling according to subjects general characters, significant differences were found in the areas of age, occupation, whether or not they have taken healthrelated lectures, family and geriatric diseases of family members or relatives. The above study results tell that the self-efficacy feeling has a great effect on their putting the health-promoting activities into practice, in daily life.

자살 시도에 영향을 미치는 정신사회적 요인

허지원,최광연,이분희,심세훈,양종철,김용구 大韓神經精神醫學會 2007 신경정신의학 Vol.46 No.2

Objectives : A lots of studies have investigated the psychosocial characteristics of suicidal attempters in order to find eff-cient coping strategy and treatment intervention. The purpose of this study was to examine the psychosocial factors affecting the suicide attempt in psychiatric patients. Methods : Two hundred eighty patients who attempted suicide and admitted in the emergency room in 3 university hospitals from December 2003 to May 2006 were recruited in this study. The patients were interviewed using Structured Clinical Inter-view for DSM-IV Axis I disorder (SCID-RV), Lethality of Suicide Attempt Ratings Scale (LSARS) and Risk-Rescue Rating system (RRR). Results : The suicide attempters have psychosocial characteristics of female preponderance, age 20-39, high school in educational level, married in marriage condition, inoccupation, house makers in occupation. Most of attempters used the non-severe methods such as drug ingestion or wrist cutting, and selected home as the place of attempt. The delay until discovery was less than 4 hours for most suicide attempters, and the rate of asking for help directly or dropping clues were high. Major depressive disorder was the most common psychiatric illness among attempters. Conclusions : The present study would be the early stage to explore the risk factor and protect factor of suicidal attempt. This study suggests that the closed observation to potential-suicidal attempter in daily life is critical to protect against the fatal results due to suicidal attempts.

분무 열분해에 의한 Ba1-xSrxFe12O19계 자성 미립자의 제조 및 특성 변화

김동현,김광만,김경남,최세영,심인보,이용근 大韓齒科器材學會 2004 대한치과재료학회지 Vol.31 No.1

Magnetic particles with alternating magnetic field are expected to be useful as thermoseeds in hyperthermic cancer treatment, since they can be targeted and confined to the cancer site. Hard magnetic ferrites such as barium ferrite and strontium ferrite are good candidates for thermoseeds of hyperthermia because of their great hysteresis loss. In order to verify the effect of Sr-substitution to Ba-site, synthesized Ba1-xSrxFe12O19 microspheres with various compositions through spray pyrolysis followed by sol synthesis using barium nitrate, strontium acetate and iron nitrate. Coercive force was increased with increasing substitution amount of Sr whereas magnetic saturation was almost constant. Spherical microspheres with average diameter of 11.7~17.0 ㎛ were produced by spray pyrolysis at 400~1000℃. The mean size was dependent on the pyrolysis temperature and flow rate of carrier gas. In order to obtain a single phase of SrFe12O19 crystal, the spray pyrolyzed spheres were undertaken subsequent heat-treatment above 1000℃. During the subsequent heat-treatment, however, spherical microspheres were agglomerated because of necking between particles. Further study has to be continued working on synthesis of pure SrFe12O19 crystal by means of improvement of pyrolysis temperature scheme and extend the heating period.

한국인 두경부암종 환자에서 Cytochrome P450 1A1,2E1 및 N-acetyltransferase 2 효소의 다형성 분석에 따른 유전적 감수성에 대한 연구

심광섭,김태균,우순섭,이영수,공구 대한악안면성형재건외과학회 2000 Maxillofacial Plastic Reconstructive Surgery Vol.22 No.4

Individual genetic susceptibilities to cancers may result from several factors including differences in xeno-biotics metabolism to chemical carcinogens, DNA repair, altered oncogenes and suppressor genes, and environmental carcinogen exposures. Among them, genetic polymorphisms of metabolizing enzymes to chemical carcinogens have been recognized as a major important host factors in human cancers. They have two main types of enzymes: the· phase I cytochrome P-450 mediating enzymes (CYPs) and phase Ⅱ conjugating enzymes. The purpose of this study is to determine the frequencies of genotypes of phase I (CYP1A1 and CYP2E1) and phase Ⅱ (NAT2) metabolizing enzymes in healthy control and head and neck cancer patients of Korean and to identify the relative high risk genotypes of these metabolizing enzymes to head and neck cancer in Korean. The author has analyzed 132 head and neck cancer patients and 113 healthy controls using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) . The results were as following; 1.The frequencies of genotypes of CYP1A1, CYP2El and NAT2 in healthy control were as following; CYP1A1 exon 7 polymorphism; Ile / Ile: Ile/Val: Va1/Va1 = 59.3%: 36.3%: 4.4% CYP2El Pst I polymorphism, C1/Cl: C1/C2: C2/C2 = 61.1%: 32.1%: 6.2% NAT2 polymorphism; F/F: F/S: S/S = 43.4%: 48.7%: 8.0% 2.In analysis of phase I enzyme, Val/Val genotype in CYP1A1 exon 7 polymorphism and C2/C2 genotype in CYP2El Pst I polymorphism were associated with relative high risks to head and neck cancers (Odds' ratio: 2.09 and 1.37, respectively). 3.Among the genotypes of NAT2 enzyme polymorphism, S/S genotype of NAT2 enzyme had 1.03 times of relative risk to head and neck cancers. 4.In combined genotyping of CYP1A1, CYP2El, and NAT2 enzymes polymorphisms, the patients with Va1/Va1 and Cl/Cl, C2/C2 and fast acetylator, and Va1/Va1 and fast acetylator had higher relative risks than the patients with each baseline of combined genotypes (Odds' ratio: 2.82, 1.98 and 2.1, respectively) . These results suggest the combined genotypes of Va1/Va1 and Cl/Cl , C2/C2 and fast acetylator, and Val/Val and fast acetylator were more susceptible to head and neck cancers in Korean. And genotyping of metabolizing enzymes could be useful for predicting individual susceptibility to head and neck cancer.

Role of the renin-angiotensin system in hepatic fibrosis and portal hypertension

( Kwang Yong Shim ),( Young Woo Eom ),( Moon Young Kim ),( Seong Hee Kang ),( Soon Koo Baik ) 대한내과학회 2018 The Korean Journal of Internal Medicine Vol.33 No.3

The renin-angiotensin system (RAS) is an important regulator of cirrhosis and portal hypertension. As hepatic fibrosis progresses, levels of the RAS components angiotensin (Ang) II, Ang-(1-7), angiotensin-converting enzyme (ACE), and Ang II type 1 receptor (AT1R) are increased. The primary effector Ang II regulates vasoconstriction, sodium homoeostasis, fibrosis, cell proliferation, and inflammation in various diseases, including liver cirrhosis, through the ACE/Ang II/AT1R axis in the classical RAS. The ACE2/Ang-(1-7)/Mas receptor and ACE2/Ang-(1-9)/AT2R axes make up the alternative RAS and promote vasodilation, antigrowth, proapoptotic, and anti-inflammatory effects; thus, countering the effects of the classical RAS axis to reduce hepatic fibrogenesis and portal hypertension. Patients with portal hypertension have been treated with RAS antagonists such as ACE inhibitors, Ang receptor blockers, and aldosterone antagonists, with very promising hemodynamic results. In this review, we examine the RAS, its roles in hepatic fibrosis and portal hypertension, and current therapeutic approaches based on the use of RAS antagonists in patients with portal hypertension.

High Dose of FGF-2 Induced Growth Retardation via ERK1/2 De-phosphorylation in Bone Marrow-derived Mesenchymal Stem Cells

Kwang Yong Shim,Fatema Tuj Saima,Young Woo Eom 대한의생명과학회 2017 Biomedical Science Letters Vol.23 No.2

Fibroblast growth factor (FGF)-2 is one of the most effective growth factors to increase the growth rate of mesenchymal stem cells (MSCs). Previously, we reported that low dose of FGF-2 (1 ng/ml) induced proliferation of bone marrowderived mesenchymal stem cells (BMSCs) through AKT and ERK activation resulting in reduction of autophagy and senescence, but not at a high dose. In this study, we investigated the effects of high dose FGF-2 (10 ng/ml) on proliferation, autophagy and senescence of BMSCs for long term cultures (i.e., 2 months). FGF-2 increased the growth rate of BMSCs in a dose dependent manner for a short term (3 days), while during long term cultures (2 months), population doubling time was increased and accumulated cell number was lower than control in BMSCs when cultured with 10 ng/ml of FGF-2. 10 ng/ml of FGF-2 induced immediate de-phosphorylation of ERK1/2, expression of LC3-II, and increase of senescence associated β-galactosidase (SA-β-Gal, senescence marker) expression. In conclusion, we showed that 10 ng/ml of FGF-2 was inadequate for ex vivo expansion of BMSCs because 10 ng/ml of FGF-2 induced growth retardation via ERK1/2 de-phosphorylation and induction of autophagy and senescence in BMSCs.