Effect of Graphene on Growth of Neuroblastoma Cells

( Park,Hye Bin ),( Hyo Geun Nam ),( Hong Gi Oh ),( Jung Hyun Kim ),( Chang Man Kim ),( Kwang Soup Song ),( Kwang Hwan Jhee ) 한국미생물 · 생명공학회 2013 Journal of microbiology and biotechnology Vol.23 No.2

The unique properties of graphene have earned much interest in the fields of materials science and condensedmatter physics in recent years. However, the biological applications of graphene remain largely unexplored. In this study, we investigated the conditions and viability of a cell culture exposed to graphene onto glass and SiO2/Si, using a human nerve cell line, SH-SY5Y. Cell viability was 84% when cultured on glass and SiO2/Si coated with graphene as compared with culturing on polystyrene surface. Fluorescence data showed that the presence of graphene did not influence cell morphology. These findings suggest that graphene may be used for biological applications.

한국(韓國)GSK 상임고문최현식(常任顧問崔賢植)의 기업활동(企業活動)과 경영이념(經營理念)

박광서 ( Kwang Sur Park ),최승현 ( Seung Hyun Choi ) 아시아.유럽미래학회 2006 유라시아연구 Vol.3 No.2

The aims of this paper are to review Mr. Choi Hyun Sik``s business activities and analysis of his management thoughts. Mr. Choi Hyun Sik is now a general advisor of Galxo Smith Cline(GSK) Korea, one of the biggest pharmatical company in the world. And he is one of the toppest chief executive officer in Korea. He was passed as a semi-salesman, one of 3D jobs at that time, by the Choong Wae Pharamatical Corporation in 1966 and he met Mr. Lee Chong Ho who was a section chief of planning and management section anf he is now chairman of the corporation. He worked hard with chairman Lee. At last he promoted as a CEO of Choong Wae Pharmatical Corporation in 1998 and Vice Chairman of the Company in 2004. After 38 years services, he resigned from CW in 2004. At the same time, He was selected as a general advisor of GSK Korea(President Mr. Kim Jin Ho) on April 2004. As a GA of GSK Korea, As a matter of fact, Mr. Choi concertrate into his best efforts as the fellowing points; ● Focus an execution of plan. ● Develop new strategic business opportunities ● Strengthen partnership with external stakeholders. ● Optimize commercial structure and capability ● Improve working capital management & operational excellence ● Build organizational capabilities to support growth. ● Develop people pipeline, and etc. And last, his management thoughts and stratagies are 5S and 5C. That is, 5S means Speed, System, Simplicity, Strategy and Spurt. On the other hand, 5C are Creation, Credit, Competition, Customer and Communication.

國內産 茶葉의 脂肪酸 組成에 관한 硏究

朴章鉉(Jang-Hyun Park),金正鳳(Jung-Bong Kim),金廣植(Kwang-Sik Kim) 한국차학회 1996 한국차학회지 Vol.2 No.1

Fatty acid composition was analyzed and compared in tea shoot of korean native tea plant (Camellia sinensis Var. Sinensis) picking from thirteen places and Yabukita(Camellia sinensis Var. Yabukita), an excellent Japanese variety. Results of this investigation are summarized as follow. Palmitic acid, Stearic acid, Oleic acid, Linoleic acid, Linolenic acid were confirmed thirteen places of korean native tea plant. The content of total fatty acid at Shunchang-gun tea leaves usually was the highest as 3555.3mg/100g, and that of Yong Kwang-gun tea leaves was the lowest as 2089.1mg/100g. The content of total fatty acid of Yabukita tea leaves usually was 3084.9mg/100g. The composition of fatty acid had order of C18 : 3 > C18 : 2 > C16 : 0 > C18 : 1 > C18 : 0.

전력선 통신 채녈의 단 구간 변화에 대한 분석

박종연,최원호,정광현 江原大學校 産業技術硏究所 2007 産業技術硏究 Vol.27 No.B

The power line channel has time-variant characteristics caused by various kind of electrical devices. This characteristics are synchronized with the main voltage by their own characteristics. The main factors of disturbance are the variation of the channel impedance and noises, In other papers [2] [3], the synchronous noise modeling has been achieved. But the modeling is not satisfied simultaneously with the time domain and the frequency domain and there are not any discussion about short-term variations of the channel impedance which cause to the signal fading. Therefore, this paper researched to solve problems about the signal fading by analyzing the short-term variation of the channel impedance, and proposed the synchronous noise modeling which is satisfied simultaneously in the time domain and the frequency domain.

조갑진균증에서 Ciclopirox Nail Lacquer의 치료효과

박경찬,김정애,방형돈,조광현,윤재일,김병수 대한감염학회 1995 감염 Vol.27 No.3

배경 및 목적:조갑진균증의 치료에 있어 국소용 항진균제는 조갑판을 통과하지 못하기 때문에 치료율이 낮은 것으로 알려졌으나 최근 조갑판을 통과하여 조상에서도 높은 치료농도를 유지할 수 있는 국소용 항진균제인 ciclopirox nail lacquer가 개발되었다. 이에 본 저자들은 8% ciclopirox nail lacquer 단독 도포의 조갑진균증에서의 치료 효과와 안전성을 알아 보기 위한 연구를 수행하였다. 방법:표적 조갑판의 감염된 면적의 비율이 50%이하인 경증의 조갑진균증 환자 27명을 평가 대상으로 하였으며 8% ciclopirox nail lacquer 1일 1회 도포요법으로 6개월간 치료하였다. 매 방문시마다 감염면적 비율을 기록하였고 치료 전과 치료 후 진균 검사를 시행하였다. 결과: 1)표적조갑판의 감염 면적이 치료 전 평균 28.9%에서 치료 종료시 4.7%로 통계적으로 유의하게 감소하였다(p<0.001). 2)KOH 직접 도말 검사와 진균 배양 검사에서 모두 음성인 진균학적 완치율은 치료 종료시 77.8%였다. 3)부작용으로는 3명(11.1%)에서 일시적 작열감, 조갑 감입 등을 호소하였다. 결론:경증의 조갑진균증 치료에 있어 8% ciclopirox nail lacquer는 비교적 좋은 치료 효과를 보이며 부작용이 적은 약제로 생각된다. Background: Topical antifungal agents have been ineffective in the treatment of onychomycosis because they could not reach the infected nail bed. 8% ciclopirox nail lacquer is a new topical antimycotic which is characterized by excellent penetration into the nail plate. We performed a study to assess the therapeutic efficacy and safety of 8% ciclopirox nail lacquer in the treatment of onychomycosis. Methods: We assessed 27 onychomycotic patients whose affected area of target nail plate was not more than 50%. The patients were instructed to apply 8% ciclopirox nail lacquer once daily and evaluated monthly for 6 months. Mycological examinations were done before and after the treatment. Overall efficacy, tolerability, cosmetic acceptance and adverse effects were assessed at the end of 6 months' treatment. Results: The mean percentage of affected area decreased from 28.9% to 4.7%. Mycological cure rate was 77.8% at the end of the treatment. Overall efficacy assessed by physician and patients were similar and evaluated as complete cure in 48% and 44%, respectively. Tolerability and cosmetic acceptance were excellent or good in most of the patients. No systemic adverse effects were found, but 3 patients complained of mild prickling sensation or ingrowing toe nail. Conclusions: These data suggest that 8% ciclopirox nail lacquer is safe and effective in the treatment of mild onychomycosis.

一部 農村住民의 高血壓 危險要因에 對한 疫學的 硏究

박광희,김기순,안현옥,노희경 조선대학교 부설 의학연구소 1998 The Medical Journal of Chosun University Vol.23 No.2

To find risk factors of hypertension among farmers whose age were 30 or more living at a rural area in the suburbs of Kwangju, a case-control study was done toward hypertensive patients and age-sex matched controls. Case groups were composed of mild hypertensive group of 70 patients whose blood pressure were 140/90 mmHg or more and below 160/100mmHg, and moderate hypertensive group of 54 patients whose blood pressure were 160/100mmHg or more. As control groups same number of persons were chosen from normotensive patients by age and sex frequency matching. The results are as follows : 1. No significant differences of educational level, marriage state, occupation and monthly Living expenses were found between case and control groups. 2. Among the mild hypertensive group the odds ratio for drinking more than 50 gm of alcohol daily versus non-drinker was 5.06 (95% confidence interval 〔CJ〕1.39 - 18.38): odds ratio for persons with high score to take salty food versus persons with low score to take salty food was 2.45 (95% CI 1.23-4.88): odds ratio for persons who were at sitting position for more than :t. 5 hours a day versus persons who were at sitting position for less than 4.5 hours a day was 2.26 (95% CI 1.15-4.47) respectively. 3. Among the moderate hypertensive group a trend was found to increase the odds ratios for drinking alcohol 20- 39.9 gm versus non-drinker, high body mass index (BMI) versus low BMI, high waist-hip ratio (WHR) versus low WHR, persons who took more fatty food versus persons who took less fatty food, and for persons who slept less than 7 hours or more than 9 hours a day versus persons who slept for 7 - 9 hours a day. The odds ratios for persons who worked for more than 10 hours a day versus persons who worked for less than 10 hours a day showed 0.59. But the confidence interval values were not statistical1y significant. In conclusion excess drinking. high salt intake and inadequate activity were found to be the significant risk factor for mild hypertension of the farmers. So appropriate education to change the life stile are necessary.

다중계층망에서 적응적인 채널할당 방법에 관한연구

박윤종,이치문,이광배,김현욱 明知大學校 産業技術硏究所 1999 産業技術硏究所論文集 Vol.18 No.-

This paper proposes a spatially distributed control scheme for a multi-layered cellular system to achieve flexible and high capacity wireless communication system under any traffic conditions including nonuniform traffic conditions. In the proposed system, terminals with lower mobility are connected to the wideband microcell systems to support variety of service including high bit rate data transmission and terminals with higher mobility are connected to the narrowband macrocell systems to reduce delay. To flexibly cope with temporal and spatial variations of traffic conditions, the ratio of the microcell and macrocell terminals as well as system bandwidth are adaptively and autonomously shared by both system Numerical simulation confirms that proposed scheme expressed flexible distributed location.

지능적인 퍼지 학습 알고리즘에 관한 연구

박수현,김광백 東西大學校 1996 동서논문집 Vol.2 No.1

본 논문에서는 인공지능분야에서 지능을 구현하는데 중요한 개념인 Minsky의 "Mind of Society"를 인간의 뇌를 모방하여 생리학적 뉴런구조를 바탕으로 하여 지능을 구현하려는 인공 신경망과 결합하여 지능적인 퍼지 학습 알고리즘을 제안하였다. 지능 퍼지 학습 알고리즘의 학습구조는 생리학적 뉴런 구조에서 흥분책을 분석하여 퍼지 논리학습 구조로 구성하였다. 즉, 본 논문에서 제안한 학습 알고리즘은 억제 뉴런구조를 전방향 억제 뉴런과 역방향 억제 뉴런으로 구분하여 전방향 억제 뉴런을 퍼지 논리곱 구조로 역방향 억제 뉴런구조를 퍼지 논리 부정 구조로 구성하였다.

유방암과 관련된 부수종양성 Stiff-Person Syndrome 1예

방현,이상암,최은정,김광국,박성혜 대한신경과학회 1999 대한신경과학회지 Vol.17 No.5

Escherichia coli에서 발현된 재조합 인간 상피세포 증식인자의 정제 및 특성

박세철,유광현 한국미생물생명공학회 ( 구 한국산업미생물학회 ) 1996 한국미생물·생명공학회지 Vol.24 No.4

E. coli BL21(DE3, pYHB101)을 이용한 rhEGF의 최적 생산조건은 25℃, 48시간 배양에서 변형된 MBL 배지에 glucose를 10g /l 첨가한 배지로 배양한 경우이었고 유도물질로 1mM IPTG를 사용하여 2시간 배양 후에 유도 배양하였을 때 최대 rhEGF가 68.7 mg/l가 발현되었다. 배양액으로부터 Amberlite XAD-7, ultrafiltration, DEAE Sepharose column chromatography 정제 과정을 거쳐 rhEGF가 정제되었으며 이때 정제도는 267배이었으며 66.6%의 회수율을 보였다. 정제된 rhEGF는 HPLC 분석 결과 2개의 분획으로 나누어졌으며 N말단 아미노산 서열 분석결과 Asn-Ser-Asp-Ser-Glu-Cys-Pro-Leu-Ser-His로 나타났다. 또한 5'-bromo-2'-deoxy-uridine (BrdU)의 삽입에 의한 DNA 생성능을 조사하였다. 상기의 결과로 E. coli BL21(DE3, pYHB101)에 의하여 생산된 rhEGF는 nhEGF와 동일한 것으로 추정되었다. Recombinant human epidermal growth factor (rhEGF) was produced strain was cultured at 25℃ for 48 hours in the modified MBS medium containing 10 g/l glucose with 1 mM IPTG induction at 2 hours after inoculation. The rhEGF was purified upto 267 folds by Amberlite XAD-7 chromatography, ultrafiltration, and DEAE Sepharose fast fow ion exchange chromatography with an overall yield of 66.6%. The purfied rhEGF was further separated into two fractions by HPLC. The N-terminal amino acid sequence of the second fraction was Asn-Ser-Asp-Ser-Glu-Cys-Pro-Leu-Ser-His. The effect of rhEGF on the DNA synthesis was examined using in vitro biological assay based on the incorporation of 5'-bromo-2'-deoxy-uridine (BrdU). The purified rhEGF shows no difference with natural human epidermal growth factor (nhEGF) in N-terminal amino acids residues and biological activity. From the results, we concluded that rhEGF produced from E. coli harboring the plasmid pYHB101 was apparently the same as nhEGF.