뇌동맥류 환자의 임상분석

강삼석,박중욱,류재광,신동철,김봉환 全南大醫大 1983 전남의대학술지 Vol.20 No.1

한국산 긴몰개(Squalidus gracilis majime, Cyprinidae)의 난발생

박경서,홍영표,문운기,최신석,안광국 한국육수학회 2005 생태와 환경 Vol.38 No.1

한국산 긴몰개 (Squalidus gracilis majimae)의 난발생 과정을 연구하기 위하여, 긴몰개의 친어와 생태적 조사는 충청남도 보령시에 위치한 보령댐과 웅천천에서 실시하였다. 자연 산란에 의해 수정된 난과 배 발생의 형태학적 설명은 다음과 같이 요약된다. 수정란의 형태는 둥글고, 점착성이 있으며 투명하였고, 수정란의 평균직경은 2.9±0.3 ㎜ (n = 30)였고, 유구가 존재하지 않았으며, 수정란의 발생은 수온 26±1.5℃에서 관찰되었다. 수정 후 20분에 배반이 형성되었으며,48분 뒤 2세포로 나뉘어졌고, 포배기는 수정 후 5시간 40분에 나타났으며, 낭배기는 수정 후 6시간 55분에 관찰되었다. 배체의 형성은 수정 후 12시간 58분부터 시작되었고, 안포와 9개의 체절이 수정 후 17시간 05분에 발견되었다. 수정 후 23시간 30분에 이포가 생겼으며, 25~26개의 체절과 함께 안포안의 렌즈가 수정 후 32시간 35분에 발견되었다. 수정 후 37시간 27분에 뇌의 분화와 함께 배체가 꿈틀거리기 시작했으며, 심장박동과 안포내의 흑색소포가 수정 후 44시간 46분에 발견되었다. 수정 후 50시간 36분 가슴지느러미의 형성과 함께 몸 부분의 흑색소포가 형성되는 것을 끝으로하여 수정 후 57시간 49분에 수정란은 부화되었다. 이때 갓 부화된 자어는 전장이 3.3±0.2 ㎜(n= 120)이었다. 본 연구 결과는 최근 수질오염 및 종 다양성 감소가 시급한 상황에서 종 및 개체군 보존에 기여할 것으로 사료된다. This study was conducted, based on the field survey and laboratory observations, to elucidate egg developmental processes and their characteristics of the Korean slender gudgeon, Squalidus gracilis majimae. For the experiments, the mature adults were collected at the Woongcheon-Cheon Stream and Boreung Reservoir located in Boreung City, Chungnam Province and eggs were obtained from the natural spawning area. Morphological characteristics of the egg and embryonic development were su㎜arized as follows: The shape of the fertilized egg was spherical, adhesive and transparent. The fertilized egg was 2.9±0.3 ㎜ (n = 30) in mean diameter under water temperature of 26 ± 1.5℃, light white in color and had no oil droplets. After 20 minutes from the time of fertilization, a blastodisc was formed and divided into two cells at 48 minutes after fertilization. The blastular stage occurred at 5 hours 40 minutes after fertilization and the gastrular stage was detected at 8 hours 41 minutes after fertilization. The beginning of embryo formation was observed at 12 hours 58 minutes after fertilization and optic vesicles and 9 somites were discovered at 17 hours 05 minutes after fertilization. Differentiation of brains and embryo wiggling were observed at 37 hours 27 minutes after fertilization. Heart beating and the formation of melanophores in optic vesicles were detected at 44 hours 46 minutes after fertilization. The formation of pectoral fins and melanophores in the body were discovered at 50 hours 36 minutes after fertilization. Hatching occurred at 57 hours 49 minutes after fertilization. The newly hatched larvae were 3.3 ±0.2 ㎜ (n = 120) in total length. We believe that these results may contribute the species and population conservations under the situation of accelerated water pollution and the decreases of its diversity.

성균관대학교 물리학과 방음실의 설계 및 제작

朴興秀,尹錫旺,李聖壽,朴光俊,吳世珍 성균관대학교 기초과학연구소 1986 論文集 Vol.37 No.1

An anechoic chamber was constructed to make to condition of acoustic free field at the Department of Physics, Sung Kyun Kwan University. In this paper the criteria for the design and construction of the anechoic chamber are described. 48cm long wedges of density 15.3kg/㎥ glasswool attached to the 40×20×20㎤ base were used for the lining of anechoic chamber with the cutoff frequency of 125Hz. The interior space of the chamber is 3.56m long, 3.38m wide and 2.37m high.

남자 펜싱선수의 연령에 따른 운동반응연구

박인기,우운수,현광석 忠南大學校體育科學硏究所 1997 體育科學硏究誌 Vol.15 No.1

The purpose of this study was to identify the difference between the Whole Body Reaction Time at offensing movement and that at defencing movement among three age groups of male fencing players. The subjects of this study were twenty-eight male fencing players in Taejon City(10 middle school players, 10 high school players and 8 collegiate players). Base upon the results of this study, the following conclusions were obtained. 1. Whole Body Reaction Time at offensing and defencing movement showed fast record in the order of high school group, college group and middle school, but there was no significant difference. 2. Whole Body Reaction Time at offensing movement showed fast record in the order of college group, high school group and middle school. And there was significant difference between college·high school group and middle school group in the comparison of offensing WBRT. 3. Whole Body Reaction Time at defencing movement showed fast record in the order of college group, high school group and middle school. And there was significant difference between college·high school group and middle school group in the comparison of defencing WBRT. 4. WBRT at offensing movement was faster than that of defensing movement. But there was no significant difference between offense movement and defense movement in the comparison of WBRT.

반응표면분석법에 의한 동충하초 첨가 증편 제조의 최적화

박금순,윤광섭,황성희,조현정,김정숙 한국조리과학회 2003 한국식품조리과학회지 Vol.19 No.4

To optimize the preparation process of Jeung-Pyun, an experiment was derived, using a central composite design, to find the optimal mixing conditions. The addition of Paecilomyces japonica powder, the volume of Tak-Ju and the second fermentation time were independent variables, with the pH, volume, color, sensory and texture properties of the Jeung-Pyun selected as response variables. As the Paecilomyces japonica powder and fermentation time were increased, the b values and volume after the second fermentation also increased. The color of the Jeung-Pyun became deeper with increases in the Paecilomyces japonica powder and fermentation time, and was deepest on the addition of 30% Tak-Ju. The pH increased with increasing fermentation time of the Jeung-Pyun, and on the addition of 3% Paecilomyces japonica rapidly increased more than with the other groups. The optimal mixing conditions for the best quality Jeung-Pyun, with restricted conditions above a b value of 3, a color intensity above 3, and a volume of 40㎖ and a pH of 4.02~4.04 after the second fermentation, were 4.0%, 20% and 45 mins for the Paecilomyces japonica powder, Tak-Ju and second fermentation time, respectively.

대장암환자에서 자연살해세포의 변화

김광호,심강섭,박응범,강은숙,홍기숙 대한대장항문학회 1998 Annals of Coloproctolgy Vol.14 No.3

단기배양을 통한 말초혈액 CD34 양성세포의 체외증폭

박상준,김철희,배광봉,김현수,김종숙,윤환중,조덕연,김삼용 충남대학교 의과대학 지역사회의학연구소 1997 충남의대잡지 Vol.24 No.1

Background: It is suggested that clinical practice in the areas of bone marrow transplantation and gene therapy might rely on the ex vivo expansion of hematopoietic stem/progenitor cells. However, the condition for ex vivo expansion of hematopoietic progenitor cells is not well established. The authors pursued a series of experiments to define the proper conditions for the expansion of hematopoietic cells in the short-term liquid suspension culture of mobilized peripheral blood CD34+ cells. Methods: 1.0ml cultures were initiated with 9×10^3 PB CD34+ cells, which were isolated from PB mononuclear cells (MNCs) by high-gradient cell sorting, in 12 well plates with the various combinations of hematopoietic growth factors(HGF). The following recombinant human HGFs were used: stem cell factor(SCF) 100ng/ml, granulocyte colony-stimulating factor(G-CSF) 100ng/ml, GM-CSF(granulocyte, macrophage colony-stimulating factor) 100ng/ml, interleukin-1 beta(IL-1B) 1ng/ml, interleukin-3(IL-3) 20ng/ml, interleukin-6 (IL-6) 100ng/ml. At the end of culture, colony-forming cells were evaluated by semisolid clonogenic assay. Results: 1) Using the high-gradient magnetic sorting system, CD34^+ cells were isolated with a yield of 40 3% 2) In 7 day culture of PB CD34^+ cells(9×10^3 cells), nucleated cells expanded mean 10×10^3(range, 9 to 20×10^3) with the addition of SCF alone, 35×10^3(range, 10 to 60×10^3) with SCF plus G-CSF plus GM-CSF, and 130×10^3(range, 40 to 300×10^3) with the combination of SCF, G-CSF, IL-1, IL-3, IL-6, GM-CSF. In 14 day culture, nucleated cells expanded 10×10^3 to 1,860×10^3 with combination of human hematopoietic growth factors. 3) In 10 day culture without medium change of PB CD34^+ cells, CFU-GM numbers expanded 16. 5 fold(range, 7 to 59 fold) with the addition of SCF plus G-CSF plus Il-1 plus IL-3, 31.3 fold(range, 20.5 to 101.1 fold) with the combination of SCF, G-CSF, IL-1, IL-3, GM-CSF. In 14 day culture with or without medium change of PB CD34^+ cells was inferior to 10 day culture for CFU-GM expansion. 4) There was no significant difference for CFU-GM expansion between five growth factors(SCF,G-CSF,IL-1,IL-3,GM-CSF) and six growth factors(five growth factors plus IL-6). Conclusion: The authors could confirm that short-term suspension culture of peripheral blood CD34+ cells could expand hematopoietic progenitor cells. Ten-day culture with medium change of CD34+ cells with the addition of five growth factors, i.e. SCF, G-CSF, IL-1B, IL-3, and GM-CSF, might be the most efficient in this system.

Polytertrafluoroethylene의 직경에 따른 신생내피의 조직학적 변화에 대한 실험적 연구

박해일,이광석 고려대학교 의과대학 1998 고려대 의대 잡지 Vol.35 No.2

In synthetic arterial grafts the regeneration and proliferation on the inner surface of graft is a part of the normal healing process. However, there are controversies in the source of these cells and the pattern of proliferation. Many investigators have suggested that endothelium might be derived from arterial sources at the ends of grafts, capillaries growing through the graft matrix from the outside, and endothelial cells can circulate in the blood and settle onto the luminal surface. The purpose of this study was to investigate the patency rates and the histologic findings of neoendothelialization according to the diameter of polytetrafluoroethylene(PTFE) which is the most widely used for synthetic graft. Ten mongrel dogs, weighing 20 to 25kg, were used for this study. Under the operating microscope, all grafts were implanted in both femoral arteries of dogs by interrupted end-to-end microanastomosis. They were divided into two groups according to the diameter of implanted PTFE. Each group comprised five dogs. The lengths of implanted PTFE were 20mm in both groups. All implanted grafts were 25㎛ in fibril length and 0.39mm in wall thickness. The internal diameter of graft in group Ⅰ was 3mm and that of graft in group Ⅱ was 5mm. Two grafts of one dog per group were harvested at 1, 2, 4, 8, and 12 weeks after implantation respectively. All PTFE grafts were observed for patency rates and the histologic findings with light microscope and scanning electron microscope. The results obtained were as follows : 1. The patency rate was higher when the diameter of implanted PTFE was larger than that of recipient vessel, and the patency rate in grafts without infection was higer than that in grafts with infection. 2. Light microscopically, in group Ⅰ, a few cells of neointima is noted at the end of the inner surface of PTFE at 1 week after implantation and the histologic findings could not be observed due to occlusion of grafts at 2, 4, 8, and 12 weeks after implantation. In group Ⅱ, the neointimal lining is advanced across the anastomosis at 1 week after implantation and the histologic findings could not be observed due to occlusion of grafts at 2 and 8 weeks after implantation. At 4 weeks after implantation, neointimal lining was more advanced to the midportion of implanted PTFE. At 12 weeks after implantation, the entire inner surface of implanted PTFE was lined completely by neointima and subintimal tissue. 3. Scanning electron microscopically, in group Ⅰ, the inner surface of implanted PTFE was lined by amorphous fibrin layer at anastomosis site at 1 week after implantationin and the histologic findings could not be observed due to occlusion of grafts at 2, 4, 8, and 12 weeks after implantation. The histologic findings of group Ⅱ were similar to those of group Ⅰ at 1 week after implantation and could not be observed due to occlusion of graft at 2 weeks after implantation. At 4 weeks after implantation, flat layer like normal endothelium was noted at anastomosis site. At 8 weeks after implantation, flat neointima was noted in the midportion of implanted PTFE. However, the inner surface of implanted PTFE was still noted at 12 weeks after implantation. 4. Neoendothelium was noted only in the patent cases with neointimal covering of the inner surface of PTFE. In conclusion, at 1 week after implantation neoendothelium started to grow from both anastomoses and at 8 weeks neoendothelium was formed in the entire inner surface of implanted PTFE. Because PTFE is not elastic like normal vessel, PTFE with larger diameter than that of recipient vessel is recommended as the grafts fot implantation.

다운증아 어머니의 기대성향이 비계화 교수와 문제해결학습에 미치는 효과

여광응,윤숙경,박현옥 한국특수교육문제연구소 2003 특수교육저널 : 이론과 실천 Vol.4 No.1

이 연구는 어머니-다운증아의 상호작용을 통한 문제해결과정에서 어머니의 기대성향이 어머니 교수중재의 양과 수준에 미치는 영향과 다운증아의 적목피라미드 문제해결과제에 미치는 효과를 밝히는데 두고 다운증아 어머니의 기대성향에 따른 어머니의 비계화 교수와 아동의 문제해결학습간의 관계를 탐색하고자 하는 것으로,만 6세인 다운증아 남아 3 명과 여아 3명인 전체 6 명 그리고 그 어머니들을 대상으로 하였다. 어머니-아동의 문제해결상호작용과정을 비디오로 촬영하여,어머니의 교수중재는 중재 수준,중재 양,유관 및 비유관 여부를 중심으로 그리고 아동의 문제해결은 성패를 중삼으로 양적 · 질적 분석을 병행 하였다. 연구결과 어머니의 기대성향이 긍정적일수록 아동의 문제해결 성패에 기반하여 중재 양과 중재 수준을 조절하는 유관 중재를 많이 사용하고 문제해결학습에서 부정적 기대성향의 다운증 자녀보다 더 긍정적 학습결과를 보인다. 또한 어머니의 비계화 교수 변인과 자녀의 문제해갤학습 변인사이에는 상호 관련작 양방향성 효과가 있다. The purpose of this study was to examine the relationships between maternal expectancy and maternal use of scaffolded instruction and problem-solving skills of children with Down syndrome. For this purpose, three boys and three girls with Down syndrome whose age were six years old and their mothers participated in this study. The mother-child interaction regarding problem-solving tasks was videotaped and analyzed. The qualitative and quantitative analyses were conducted to find out the condition and quantity of scaffolded instruction used by mothers, and degree of their contingency of the tasks. In addition, it was analyzed whether these children resolved given problems successfully or not. As a result, it was found that the mothers who had more positive expectancy relied more on contingent intervention that regulated the quality and quantity of instructions based on the success or failure of their child' s problem solving tasks, and that their children produced better learning outcome than the children of the mothers who had more negative expectancy. In addition, two-way interrelationships between the scaffolded instruction variable of the mothers and their children ’ s problem-solving learning variable were found.

도재브라켓의 제거방법에 따른 법랑질표면의 주사전자현미경학적 관찰

박미숙,윤영주,김광원 대한치과교정학회 1996 대한치과교정학회지 Vol.26 No.5

도재브라켓의 제거방법에 따라 법랑질표면에 미치는 영향을 비교 평가하기 위하여 80개의 발거된 소구치를 대상으로 통법에 의해 도재브라켓을 부착시키고 일주일 후 각각 40개의 기계적 방법에 의한 제거군과 전기열전도 방법에 의한 제거군으로 구분하여 도재브라켓의 제거를 유도한 후, 그 탈락양상 및 도재브라켓과 법랑질 표면의 주사전자현미경 관찰을 시행하였으며, 전기열전도 방법에 의한 제거군을 다시 각각 10개씩 고속 tungsten carbide bur에 의한 연마군, 저속 sof-lex disc에 의한 연마군, 고속 resin polishing bur에 의한 연마군, 그리고 초음파 치속제거기에 의한 연마군으로 분류하여 잔여레진의 연마를 시행한 후, 잔여레진의 연마를 시행한 후, 잔여레진의 평가에 의해 다음과 같은 결론을 얻었다. 1) 결찰와이어 절단용 겸자를 이용한 도재브라켓의 기계적 제거시, 0.69의 평균 잔여레진 부착지수를 보임으로써 법랑질과 레진 경계부에서 파절이 가장 빈발한 양상을 보였다. 2) 전기열전도 방법을 이용한 도재브라켓의 제거시 2.19의 평균 잔여레진 부착지수를 보임으로써 브라켓과 레진 경계부에서의 파절이 가장 빈발한 양상을 보였다. 3) 기계식 방법으로 도재브라켓의 제거시, 법랑질표면의 주사현미경 관찰소견은 실험군의 7.5%에서 법랑질표면의 탈락과 패임 등의 손상을 보였다. 4) 잔여레진의 제거의 고속 resin polishing bur 사용군에서 가장 적은 잔여레진막을 보였다. The purposes of this study were to evaluate and compare the frequency of ceramic bracket fracture, frequency of enamel fracture, bond fracture site, adhesive remnant index after mechanical and electrothermal debracketing, to evaluate effectiveness of high and low speed rotary instrument and ultrasonic instrument during residual adhesive remnants removal, and to measure resin film surface(percentage) using by image analyser(Leco 300). Bond fracture site, bracket fracture, and enamel surface damage were examined by scanning electron microscope. The following results were obtained: 1. In the mechanical debracketing group, the bond failed predominantly at enamel-adhesive interface with the bulk of adhesive remaining on bracket base. 2. In the electrothermal debracketing group, the bond failed predominantly at adhesive-bracket interface with the bulk of adhesive remaining on enamel surface. 3. The most effectiveness of residual resin removal was obtained by means of the resin polishing bur and the order of scratch formation was the precedure using tungsten carbide bur, ultrasonic scaler, sof-lex disc, and polishing bur. 4. The order of the resin film surface percentage was ultrasonic scaler, tungsten carbide bur, sof-lex disc, and resin polishing bur.