운동실조를 동반하지 않은 급성외안근마비 1례

신대수,이승회,이한샘,박성욱,박현영,장혁,김요식,조광호 圓光大學校 醫科學硏究所 2004 圓光醫科學 Vol.19 No.2

등가영역 적분법에 의한 균열선단 적분계수의 산정

신성진,홍종현,우광성 嶺南大學校 工業技術硏究所 1996 工業技術硏究所論文集 Vol.24 No.1

In this study, an equivalent domain integral (EDI) method is presented to estimate the crack-tip integral parameter, J-value, for two dimensional cracked elastic bodies which may quantify the severity of the crack-tip stress fields. The conventional J-integral method based on line integral has been converted to equivalent area or domain integrals by using the divergence theorem. It is noted that the EDI method is very attractive because all the quantities necessary for computation of the domain integrals are readily available in a finite element analysis. The detail and implementation of EDI method are extened to both h-version finite element model with 8-node isoparametric element and p-version finite element model with high order hierarchic element using Legendre type shape fuctions. The variations with respect to the different path of domain integrals from the crack-tip front and the choice of S-function have been tested by several examples.

Quinacrine 형광을 이용한 토마토 뿌리조직 마이크로솜의 수소이온이동 활성측정

신대섭,조광현,김영기 충북대학교 한국과학재단 지정 첨단원예기술개발 연구센터 2002 연구보고서 Vol.6 No.-

Quinacrine은 수소이온 농도변화에 민감한 형광 probe로서 양성자와 결합하지 않은 형광형이나, 양성자와 결합한 비형광형으로 존재한다. 따라서, quinacrine은 H+-ATPase에 의한 수소이온이동 활성측정에 이용된다. 본 연구에서는 토마토 뿌리조직에서 분리한 마이크로솜에서 quinacrine의 형광성을 이용한 H+-ATPase 활성측정의 최적 조건을 조사하였다. Quinacrine의 형광변화는 반응용액 중의 단백질 함량이 0.43㎍/㎕에서 25-26% 감소하여 10%의 quinacrine 형광을 감소시키는 데 약 100 nmol/min의 H+-ATPase 활성이 필요함을 알 수 있었다. Quinacrine의 최대 형광변화는 pH 7.0-7.2 범위와 2 mM Mg2+ 조건에서 일어났다. 이것은 기존에 보고한 H+-ATPase의 특성과 잘 일치하여, quinacrine의 형광변화가 H+-ATPase의 활성을 잘 반영하고 있음을 보인다. 원형질막 및 액포막 H+-ATPase들의 선택적 저해제인 vanadate와 NO3-는 각각의 효소에 의한 수소이온이동 활성을 저해하는데 성공적임을 확인하였다. 이상의 결과로 quinacrine이 토마토 뿌리조직에서 분리한 마이크로솜의 수소이온이동 활성측정에 유용하게 이용될 수 있음을 확인하였다. Quinacrine, a pH-sensitive fluorescence probe, which exists either as an unprotonated fluorescence form or protonated nonfluorescence form, can be used to measure the proton transport activity of H+-ATPase. Quinacrine was used to determine the optimal conditions for measuring the activity of microsomal H+-ATPases prepared from the roots of tomato plants. The amount of quinacrine fluorescence quenching obtained at 0.43 ㎍/㎕ of microsomal protein concentration was 25-26%, which shows that the enzyme activity of 100 nmol/min decreases 10% of quinacrine fluorescence. Maximal fluorescence quenching was obtained at pH 7.0-7.2 and 2 mM Mg2+. Because the activity of microsomal H+-ATPase is also maximal at these conditions, the quinacrine fluorescence well represents the activity of H+-ATPase. Vanadate and NO3-, specific inhibitors of plasma and vacuolar H+-ATPase, respectively, were successfully applied to inhibit the quinacrine fluorescence quenching mediated by the corresponding H+-ATPase. These results imply that quinacrine is a useful tool for measuring the proton transport activities of microsomes obtained from the root tissued of tomato plants.

GT rotary file을 이용한 만곡 근관형성시 작업 효율 및 file 변형 발생에 관한 연구

신주희,백승호,배광식,임성삼,윤수한,김병현 대한치과보존학회 2001 Restorative Dentistry & Endodontics Vol.26 No.5

Root canal preparation process is of utmost importance in successful treatment of root canal. Also, one of the most important purpose of the root canal preparation is to enlarge the root canal three dimensionally without changing the curvature of the root canal. However, as the curvature of the root canal increases, there are many difficulties involved in formation of optimum root canal. Therefore, in order to solve the above mentioned problems, new developments in methods of root canal preparation and equipments for such purposes were made. Recently, vigorous studies about newly introduced engine-driven nitckel-ti-tanium rotary file are conducted. As shown in research results to dates, it is well established that the use of nickel-titanium file is better suited for curved root canal than stainless steel file in maintaining the curvature of root canal and reducing the deformation of root canal. However, it is also acknowledged that there are a few discrepancies in research results according to protocol, due to failure to remove variables in experiments. In addition, although it is recommended by the manufacturer that the GT rotary file should maintain a low rotational speed of 150∼350rpm and 'light pressure' as light as not to break the lead of a pencil, academic studies about the vertical force which is not yet standardized are not sufficiently explored. Therefore, this research devised and utilized a special research equipment to standardize the appropriate range of vertical force for GT rotary file through experiments by breaking of the lead of a pencil as expressed by the manufacturer and to accurately measure factors involved through repeating and recreating the environment of root canal preparation. Forming nine experimental groups by varying the vertical forces (150g, 220g, 300g) and rpm (150rpm, 250rpm, 350rpm), the effects of changing vertical forces and rpm on working efficiency were measured in terms of time expended in root canal preparation by crown-down method using a transparent resin block with 35 degree curvature and GT rotary file (z-test). The following research using this special research equipment that involved nine experimental groups and varying the vertical force for root canal preparation from 300g which is within the normal vertical force range to 700g and 1000g which fall outside the normal rpm range. The results were as follows : 1.Analysis of the experiment results revealed that the tome spent in root canal preparation decreased as the vertical forces and rpm increased (p<0.05). Also, the effects of rpm were greater than those of the vertical forces within the normal vertical force range (β-weight test) . 2.Observation of the deformation of GT rotary file revealed that deformation increases in a direct correlation with the vertical force increase and in a reverse correlation with the rpm decrease, In the case of the vertical forces close to the normal range, the probability of GT rotary file deformation were quite different depending on the rpm changes. In the case of◎eater tier◎cal forces, the occurrences of deformation of the file were more frequent regardless of the rpm changes. 3.Deformation and breakage of file were also commonly observed in the expended time measurement experiments and GT rotary file deformation experiments in which low speed rpm (150rpm) was used and at the curved portion of the resin block. Key words : GT rotary file, Crown-down method, Working efficiency, Vertical force, Rpm, Deformation and breakage of file

Hg^2+에 의한 토마토 뿌리조직 마이크로솜 H^+-ATPase의 가역적 저해

신대섭,조광현,김영기 충북대학교 첨단원예기술개발연구센터 1999 연구보고서 Vol.4 No.-

토마토 뿌리조직의 마이크로솜 ATPase 활성에 대한 중금속의 효과를 조사하기 위하여 뿌리조직으로부터 마이크로솜을 분리하였고, enzyme-coupled assay를 이용하여 마이크로솜 이온펌프(ATPase)의 활성을 측정하였다. 여러 가지 중금속 이온들 중 Hg2+은 마이크로솜 ATPase 활성을 농도 의존적으로 저해하였으며, Gd3+ 과 Fe3+, La3+, Zn2+ 그리고 Pb2+ 등은 마이크로솜 ATPase의 활성을 현저히 저해하면서 동시에 assay에 사용된 효소를 저해하였다. 그러나, CS+과 BA2+은 마이크로솜 ATPase 활성에 영향을 미치지 않았다. Hg2+은 원형질막과 액포막에 위치하는 H+-ATPase들의 활성을 10 μM 이상의 농도에서 급격히 저해하였고, 1 mM 이상의 농도에서 완전히 저해하였으며, 두 효소들에 대한 활성저해의 Ki 값은 각각 80 , μM, 58 μM로 나타났다. Hg2+에 의해 저해된 ATPase의 활성은 DTT의 농도를 증가시킴에 따라 회복되어, HG2+에 의한 ATPase 활성저해는 가역적임을 확인하였다. 이러한 결과들은 Hg2+이 원형질막과 액포막에 위치한 H+-ATPase들을 비선택적이고 가역적으로 저해함을 보여준다. In order to characterize the effects of heavy metal ions on the microsomal ATPase activities, microsomes were prepared from the roots of tomato plant and the activity of microsomal ATPase was measured by an enzyme-coupled assay. Hg2+ inhibited the activity of microsomal ATPase in a dose-dependent manner, while Gd3+, Fe3+, La3+, Zn2+, and Pb2+ inhibited not only the ATPase activity but also the activities of enzymes used in the assay. However, Cs+ and Ba2+ showed no significant effect. Hg2+ inhibited the activities of both plasma membrane and vacuolar membrane H+-ATPases. In the dose-response to Hg2+, the activities of both microsomal H+-ATPases were severely inhibited at the concentration of Hg2+ above 10μM and were completely inhibited at 1 mM Hg2+. Apparent Ki values of Hg2+ on the inhibitions of plasma membrane and vacuolar membrane H+-ATPases were 80 μM and 58 μM, respectively. The Hg2+-induced inhibitions were reversible since the addition of dithiothreitol completely reversed the inhibitory effects of Hg2+.These results suggest that the inhibitory effects of Hg2+ on both plasma membrane and vacuolar membrane H+-ATPases are nonselective and reversible.

初期 韓國敎會의 査經會를 통한 信仰敎育에 관한 小考

신현광 안양대학교 신학연구소 1996 神學硏究 Vol.4 No.-

고혈압 환자에서 Leukoaraiosis 정도와 망막증과의 연관성 : 예비연구 Preliminary Results

신동익,한현정,서대희,이광훈 關東大學校 醫科大學 醫科學硏究所 2002 關東醫大學術誌 Vol.6 No.1

Changes in the cerebral white matter (leukoaraiosis;LA) are detected with increasing frequency by computed tomography (CT) and magnetic resonance imaging (MRI) in elderly patients with diabetes mellitus and hypertension. The purpose of this study is to determine whether the severity of retinopathy is correlated with degree of LA in patients with hypertension. Twenty-three consecutive patients with patients with hypertension admitted in the department of neurology were evaluated for degree of LA and severity of retinopathy. 8patients were 15female. The age distribution was between 52 and 85 years(mean 69.1years). The duration of hypertension was between 1 and 30 years(mean8.2 years). The degree of LA was correlated with severity of hypertensive retinopathy(p<0.05), especially in relatively young patient, short periods of disease, and female paitents(p<0.05). We can make a rough estimation of degree of LA with fundus examination without CT or MRI evaluations in hypertensive patients, especially in relatively young and female patients with short durations of disease. However, more broad study should be made for these value and relationship.

인간화를 위한 기독교 교육에 대한 우리의 이해

신현광 안양대학교 신학연구소 1995 神學硏究 Vol.3 No.-

양측 상완 원위부에 발생한 Kimura씨 병의 치험 1례 : 증례보고 A case report

신현대,이광진,이승진 충남대학교 의과대학 지역사회의학연구소 1997 충남의대잡지 Vol.24 No.2

Angiolymphoid hyperplasia with eosinophilia(ALHE), or Kimura's disease, is an uncommon lesion of unknown etiology with a predilection for skin of the head and neck region. It usually presents as one or a few smooth-surfaced nodules which are often asymptomatic. Histologically the nodules reveal two components : abnormal proliferating vessels and a cellular infiltrate consisting of lymphocytes and eosinophils, involving both the dermis and the subcutaneous tissue. A case of sixteen years old boy is presented of an unusual lesion around the elbow joint followed from December 1994 to September 1997 at Chungnam national university hospital. We had marginally resected symetrically developed lesion around elbow joint measuring 3x5x7㎝, and then medicated with prednisolone 60mg per oral medication to postoperative 10 days.

12주 근력강화훈련 동안 표면근전도 신호의 특성

신화경,조상현,차광석 한국전문물리치료학회 2003 한국전문물리치료학회지 Vol.10 No.1

Ⅰ. 서론 Ⅱ. 연구방법 1. 연구대상 2. 실험설계 3. 근력강화훈련 4. 최대 근력의 평가 5. 표면근전도 신호 수집 가. 장비 설정 나. 표면근전도 신호 수집 6. 신호 처리 가. 주파수 스펙트럼 분석 나. 적분EMG 7. 통계분석 가. 정규화 나. 통계분석 Ⅲ. 결과 1. 근육 둘레의 변화 2. 적분EMG와 근수축력의 변화 가. 등척성 운동 나. 등장성 운동 다. 12주 동안 근력과 적분EMG의 변화 3. 초기중앙주파수의 변화 4. 피로지수의 변화 5. 회귀직선 기울기의 변화 Ⅳ. 고찰 Ⅴ. 결론 This study tested whether repeated measurement of median frequency (MDF)-related variables could express the muscle power changes during a 12-week DeLome strengthening program, by using consecutive overlapping FFT (Fast Fourier transformation) and integrated EMG (IEMG) from surface EMG data for isometric and isotonic exercise. To evaluate the effect of training, the following were recorded every 3 weeks for the elbow flexors and knee extensors of 5 healthy male volunteers: MVC, 1RM, limb circumference, and surface EMG during isometric MVC or isotonic contraction at 10RM load. From the EMG data, IEMG and variables from a regression analysis between MDF and time were obtained. MVC, 1RM, IEMG, and initial MDF increased linearly over the training period. The fatigue index and slope of the regression line increased temporarily until the 6th week and decreased thereafter. From these results, there appeared to be enhanced neural recruitment of fast twitch fibers in the first 6 weeks and continued enhancement in the recruitment and hypertrophy of fast twitch fibers, which led to increased fatigue resistance, over the last 6 weeks. Accordingly, the MDF and IEMG analysis technique could demonstrate the effect of the program detected significant changes in both isometric and isotonic contractions. EMG analysis methods can be used to estimate the electrophysiological and histological changes in skeletal muscles during a strengthening program.