태권도 품새 수련이 아동의 심박수, 심근산소소비량, 에너지 대사에 미치는 영향

정성태,전태원,박익렬,정덕조,우재홍,이광희,이동기,강현주 서울대학교 체육연구소 2000 서울大學校 體育硏究所論集 Vol.21 No.1

The Taegeuk Poomse 1 through 8 of Taekwondo are based upon eight major branches of philosophical theories. The propose of this study was to investigate the nfluence of Taekwondo poomse trainning on heart rate, MVO2, energy metabolism in primary school students. Subjects for this study were eight male the fifth∼sixth grades students who participated in exercise program consist of Taekwondo poomse trainning in dojang which is located in Shinlim-2dong, Kwanak-Gu, Seoul(12.7± 0.5years, 150.8 ±4.6cm, 45.1 ± 9.4kg, 24.9 ± 9trainning length). The results of thet this study are as follows: 1) The average of heart rate was Taegeuk Poomse 1 through 8 of Taekwondo 90.57± 4.gbeats/min in rest and 139.7 ± 17.5beats/min in exercise. The minimum heart rate was 89.0±2.8, peak heart rate was 167.1 ±6.4 in exercise. It was ranged from 42% to 80% 2) of the max heart rate and ranged 42 to 65%HRmax. Taegeuk 1 Jang was differenced with others Jangs significantly(p<0.05), 2) The time of the Taegeuk 1 through 8 Jang of Taekwondo was 5minutes 12seconds. Heart rate was ranged from 90.6 ± 7.85beats/min to 171.8 ±8.Ibeats/min and increased linearly. 3) The average of the MVO2 was 11.1 ±0.78mmHg · bpm-3 in rest and 19.1 ± 1.2mmHg ㆍbpm-3 in exercise. MVO2 was ranged from 10.5mmHg · bpm-3 to 20.3mmHg · bpm-3. There were not difference between Taegeuk Poomses(p<0.05). 4) The energy metabolism was as follows: Mean VO2 Respiratory exchange ratio, energy consumption were 0.51(V02L/min),0.71(RQ),2.32Ckca1/min) in rest respectively. but In exercise Mean VO2 Respiratory exchange ratio, energy consumption were 0.89 ± 0.18( V 02L/min), 0.79±0.08(RQ), 4.26±0.51(kcal/min) respectively . The difference was only between Taegeuk 1 Jang and Taegeuk 3 Jang(p<0.05). This study showed that the Taekwondo Poomse trainning was proper exercise for fitness, body composition and weight control in normal, abnormal(obese) children because the Taekwondo trainning are dynamic and exciting sport.

Dimethyl-β-Cyclodextrin을 이용한 Omeprazole의 대체투여경로 개발

정미분,박승현,김하형,이광표 중앙대학교 약학연구소 1996 약학 논총 Vol.10 No.-

For the purpose of increaing the stability and bioavailability of omeprazole(OME), inclusion complexes of OME with demethyl-β-cycoldextrin(DMβCD) and hydroxypropyl-β-cyclodextrin(HPβCD) were prepared by freeze drying method. The interactions of OME with DMβCD and HPβCD in aqueous solutions and in solid states were investigated by solubility study. IR spectroscopy and DSC. The type of OME-DMβCD inclusion complex was found to be classified as Bs-type on phase solubility diagram, and the stoichiometric ratio of OME-DMβCD incousion complex was a 1:2 and stability constant was 359.46M^-1. The OME-HPβCD inclusion complex appeare to be AL type. The stoichiometric ratio was found to be 1:1, and stability constant was 34M^-1. Clear differences in a IR spectra and DSC curves were observed between the inclusion complex formation at pH 6.8. The mean dissolution time(MDT). which represents the degree of the rapidity of dissolution rate, was increased in the order of OME-DMβCD I.C(0.60) < OME-HPβCD I.C.(0.71) < OME-DMβCD P.M.(7.23) < OME-HPβCD P.M.(10.06). In order to compare simultaneously the intrinsic absorptivity of OME in the jejunum and nasal cavity of rats, the in situ nasal and jejunal perfusion method was employed as a new trial. The in situ recirculation study revealed that OME-CDs inclusion complexes rate proportionally increased the absorption of OME in nasal cavity(N) and the jejunum(J) of rats. The decreasing order of apparent OME permeability(Papp:cm/sec×10-5), corrected for surface area of absorption was as follows : 8.77±0.18(N, OME-DMβCD). > 5.97±0.17(N. OME-HPβCD) > 4.84±0.02(J. OME-DMβCD). 3.50±0.25(J. OME-HPβCD) at IPB pH 7.4. The present results suggest that OME-DMβCD inclusion complex may serve as a potential means for the nasal delivery of OME.

치환용매 변화에 따른 비대칭 Ceellulose Acetate 막의 기체투과특성

정광보,장승현,남석태 한서대학교 산학협력연구원 부설 환경연구소 2000 환경연구소 논문집 Vol.3 No.-

Gas permeation behavior was examined for asymmetric cellulose acetate membrane prepared by changing the acetone evaporation time and drying solvent. By changing the acetone evaporation time, the gas permeation rate decreased for the first 2 minutes and then increased gradually for the membrane of i-PrOH exchange drying. Gas permeation rate was in the order of He>N₂>O₂ and separation factor was about 1.9 for the membrane of 9 minute acetone evaporation. The permeation mechanism appears to be the combination of Knudsen flow and viscous flow. The effect of Knudsen flow was increased with acetone evaporation time, while the effect of viscous flow increased for the membrane of EtOH exchange drying.

ECAP 공정을 거친 1050 Al 합금 판재의 전기화학 특성에 관한 연구

정민경,최윤석,김정구,김영만,이재철,석현광 대한금속재료학회 2003 대한금속·재료학회지 Vol.41 No.8

The effect of equal channel angular pressing (ECAP) pass number on the electrochemical properties of AA 1050 (UNS A91050) in 0.1M Na₂SO₄ solution containing 100 ppm chloride were investigated by electrochemical techniques (potentiodynamic test, potentiostatic test, electrochemical impedance spectroscopy (EIS) and surface analyses (OM, SEM, EDS). The ECAPed specimens that have a various numbers of passes from N=l to N=5 were selected for electrochemical experiments in this study. The results of potentiodynamic test indicated that the breakdown potential increased with increasing the ECAP pass number. EIS measurements also showed that the corrosion resistance of the AA 1050 increased with increasing the ECAP pass number at the passivation and breakdown states. The results of surface analyses (SEM, EDS) showed that silicon-containing impurities were observed on the surface of the alloy. It was found that the size of these Si-containing impurities decreased with increasing the ECAP pass number. The Si-containing impurities induced the microgalvanic reaction(i) between the Al matrix and the Si-containing mixed oxide and (ii) between the Al matrix and the Si-containing impurities. Due to its ultrafine-grained microstructure, ECAPed AA 1050 had smaller Si-containing impurities, and reduced microgalvanic currents. Thus, in the case of ECAPed AA 1050, it has increasing corrosion resistance with increasing ECAP pass number because of its small cathodic impurities.

초기 다발성 신경병증의 진단에 족지간 신경전도검사의 유용성에 관한 연구

이광우,이성현,정재면 대한신경과학회 1998 대한신경과학회지 Vol.16 No.2

다원적 기법에 의한 중소기업의 자금조달 실태

권광현,정정현,현영하 昌原大學校 産業經濟硏究所 1996 産經硏究 Vol.13 No.-

노화가 인체 중간엽 줄기세포로부터 조골세포로의 증식 및 분화에 미치는 영향

백기현,태현정,오기원,이원영,조정기,권순용,강무일,차봉연,이광우,손호영,강성구,김춘추 대한내분비학회 2003 Endocrinology and metabolism Vol.18 No.3

연구배경: 일반적으로 골다공증과 연관된 위험인자로는 연령, 폐경, 약물, 불충분한 칼슘섭취, 만성질환 및 운동부족 등이 있는데, 특히 노화가 진행할수록 골밀도가 감소하는 것은 잘 알려져 있다. 노화와 관련하여 진행되는 골소실은 조골세포 및 전구조골세포의 기능적 결핍에 의한 골형성의 감소가 주요한 요인으로 여겨지고 있다. 그 동안 연령이 조골모 세포의 양과 조골모 세포로부터 성숙조골세포로의 분화 및 증식에 미치는 영향에 대한 일부 보고들이 있었으나 아직 일치된 견해는 없는 형편이다. 방법: 다양한 연령의 사람으로부터 골수를 채취, 중간엽 줄기세포가 포함된 단핵세포를 분리한 후 조골세포로 분화하기 좋은 조건하에서 배양하였다. 대상군은다시 젊은군과 노령군으로 구분하여 다양한 변수를 비교 분석하였다. 일차배양에서는 CFU-F를 계수하여 골수내 중간엽 줄기세포의 수를 추산하였고, 칼슘측정을 통하여 기질의 무기화 정도를 비교하였다. 계대배양후 이차배양에서는 시기별로 알카리성 포스파타제 활성도를 측정하고 오스테오칼신 mRNA의 발현을 관찰하여 젊은군과 노령군 사이의 증식능 차이를 비교하였다. 또한 이차배양 시기별로 MTT 측정을 하여 양군간에 증식능 차이가 있는지 알아보았다. 결과: 1. 일차배양 15일째에 평균 CFU-F의 수는 젊은군에서 유의하게 많았다(젊은군 148.3±28.9, 노령군 54.3±9.1, p=0.02). CFU-F의 평균면적은 젊은군에서 넓은 경향을 보였으나 통계적으로 유의하지는 않았다. 2. 일차배양 17일 경과 후 양군간에 기질 칼슘 침착정도는 유의한 차이를 보이지 않았다(젊은군 103.6±50.6, 노령군: 114.0±56.5, p=NS). 3. 이차배양 10일째에 젊은군에서 알카리성 포스파타제 활성도가 고령군에 비해 유의하게 높았다(젊은군: 935.5±115.0 U/mg, 노령군: 578.4±115.7U/mg,p.0.05). 고령군에서는 시간 경과에 따른 변화가 미약했으며 전반적으로 알카리성 포스파타제의 활성도가 젊은군에 비해 낮았다. 4. 이차배양도중 오스테오칼신 mRNA의 발현은 배양시기별로 젊은군에 비해 고령군에서 더 낮은 경향을 관찰할 수 있었다. 5. 이차배양 10일과 15일에 젊은군에서 노령군보다세포증식이 유의하게 증가된 양상을 보였다(10앓 젊은군 0.73±0.05, 노령군 0.58±0.04, p=0.05, 15일; 젊은군 0.80±0.05, 노령군 0.70±0.03, p=0.05).결론: 이상의 연구에서 저자들은 노령군에서 젊은군보다 골수 내 중간엽줄기세포의 수가 적고, 노령군에서 유래한 전구조골세포의 성숙조골세포로의 증식 및 분화가 젊은군 보다 감소해 있는 것을 관찰할 수 있었다. Background: Osteoblasts originate from osteoprogenitor cells in bone marrow stroma, termed mesenchymal stem cells (MSCs) or bone marrow stromal cells. Each MSC forms colonies (colony forming units-fibroblasts [CFL-Fs]) when cultured ex vivo. There are some reports about the age-related changes of the number and osteogenic potential of osteoprogenitor cells, but any relationship has not been clearly established in humans. In this study, we counted MSCs using CFU-Fs count and examined the proliferative capacity and differentiation potential of osteoprogenitor cells. Finally, we analyzed how these parameters varied with donor age. Methods: Bone marrow was obtained from the iliac crest of young (n=6, 27.2±8.6 years old) and old (n= 10, 57.4k6.7 years old) healthy donors. Mononuclear cells, including MSCs, were isolated and cultured in osteogenic medium. In primary culture, we compared the colony-forming efficiency of MSCs between the two groups and determined the matrix calcification. When primary culture showed near confluence, the cells were subcultured. Alkaline phosphatase activity, osteocalcin expression by RT-PCR and proliferative potential by MTT assay were examined by the time course of secondary culture. Results: At the 15th day of primary culture, the mean number of CFU-Fs was significantly higher in the younger donors (young: 148.3±28.9, old: 54.3±9.1, p=0.02) and the mean size of CFL-Fs was also larger in the younger donors than the older donors. However, matrix calcification was not different between the two groups (young: 103.6±50.6, old: 114.0±56.5, p=NS). In secondary culture, alkaline phosphatase activities were significantly lower in the older donors. The younger donors showed peak alkaline phosphatase activity at day 10, while the older donors didn't showed a remarkable peak (young: 935.5±115.OU/mg, old: 578.4±115.7U/mg, p<0.05). Total cell number as a proliferative index increased progressively during the secondary culture and a significantly greater cell number was noted in the younger donors. Osteocalcin expression was generally upregulated in the younger donors, but this was not statistically significant. Conclusion: Our study shows that the number of osteoprogenitor cells is decreased during aging and that the proliferative capacity and differentiation potential of osteoprogenitor cells seem to be reduced during aging (J Kor SOC Endocrinol 18:296-305, 2003).

골아세포 MC₃T₃-E₁의 증식과 기능에 Insulin Like Growth Factor Ⅰ, Ⅱ 및 부갑상선 호르몬이 미치는 영향

윤현구,정호연,양인명,김성운,김진우,김광원,김영설,최영길,서광식 대한내과학회 1992 대한내과학회지 Vol.42 No.1

MC₃T₃-E₁골아세포군을 이용하여 IGF-Ⅰ과 Ⅱ를 투여하고 DNA합성과 골아세포기능을 측정하기 위해 PTH에 대한 C-AMP반응정도, alkaline phosphatase 활성도를 측정하여 다음과 같은 결과를 얻었다. 1) IGF-Ⅰ과 Ⅱ는 모두 DNA합성이 어느 농도까지는 증식효과를 나타내지만 그 이후에는 억제되는 양상을 보였다. 2) PTH에 대한 골아세포의 C-AMP반응으로 증가되었지만 IGF-Ⅰ과 Ⅱ의 투여시 농도변화에 따른 의미있는 변화는 없었다. 3) 본 실험의 조건에서 alkaline phosphatase 활성도는 증가되지 않았다. 이상의 결과로 MC₃T₃-E₁ 골아세포에 IGF-Ⅰ과 Ⅱ는 세포의 증식을 자극하여 PTH는 C-AMP생성을 촉진하는 효과가 있었다. The effect of insulin-like growth factor (IGF) Ⅰ and Ⅱ on cloned MC₃T₃-E₁ cells that had osteoblastic activity was examined by [³H] thymidine incorporation into DNA, cyclic AMP responsiveness to parathyroid hormone (PTH), and alkaline phosphatase activity. IGF Ⅰ and Ⅱ stimulated the growth and proliferation of osteoblastic cells at 20ng/㎖ and 50ng/㎖, respectively. We next examined the effect of IGF-Ⅰ and Ⅱ on the expression of osteoblastic activity. Adenylate cyclase response of PTH to IGF-Ⅰ and Ⅱ-treated cells showed maximal stimulation at 10 and 50㎚PTH, respectively, but IGF Ⅰ and Ⅱ induced limited activity of osteoblastic function. In conclusion, IGF-Ⅰ and Ⅱ were potent stimulators to the growth of osteoblastic cells but did not have an immediate effect on expression of osteoblastic function in this experiment.

Syntheses of New Azo Calix[4]arenes Containing Benzo Crown Ether

장승현,정광보 大邱大學校附設 基礎科學硏究所 1998 基礎科學硏究 Vol.15 No.1

New azo calix[4]arenes, 5-benzo-15-crown-5-azo-25,26,27,28-tetrahydroxy calix[4]arene(1), and 5,17-bis(benzo-15-crown-5-azo)25,26,27,28-tetrahydroxy calix[4]arene (2) were prepared by reaction of diazonium salt of 4-aminobenzo-15-crown-5 and clix[4]arene. 4-Aminobenzo-15-crown-5 was synthesized by reduction of 4-nitrobenzo-15-crown-5, obtained from the nitration of monobenzo-15-crown-5.

보건관리 대행사업 평가도구의 타당성, 신뢰도 및 인과관계 검증에 관한 연구

박정일,조병희,구정완,김광종,김현욱,원종욱,이명숙,정문희 가톨릭대학교 산업의학센터 2001 韓國의 産業醫學 Vol.40 No.1

In order to verify the validity, reliability and relativeness of preliminary evaluation tool for the group occupational health services(GOHS), a questionnaire survey for doctors, nurses and hygienists of 18 GOSH institutions, and employers, health managers and employees of 340 enterprises was conducted. A factor analysis and correlation test for the statistical validity of the variables in the evaluation tool, a Chronbach's Alpha value for the reliability in the evaluation indices and a regression analysis for the relations of the evaluation model were performed. The results were as follows: 1.In factor analysis, thirty-three variables among 43 variables were selected as significant variables, ie 9 out of 11 for input index, 11 out of 14 for throughout index and 13 out of 18 for out put index. 2.Three evaluation(outcome) scales(evaluation by employer, health manager and employee) which composed of 16 identical items were used. 3.The Chronbach's Alpha values of input, throughout, out put and outcome indices were 0.5221, 0.5473, 0.7122 and 0.8546 respectively. 4.Regression equations of independent variables on the evaluation scales of employer, health manager and employee were all statistically significant.