Bioremediation of imidacloprid using Azospirillium biofertilizer and Rhizobium biofertilizer

Kavita Kulkarni,Aishwarya Chawan,Anand Kulkarni,Sandip Gharat 한국화학공학회 2022 Korean Journal of Chemical Engineering Vol.39 No.10

Imidacloprid is a pesticide used for agricultural purposes. Residue of pesticide in water and soil will affect the water and soil quality. Seepage out of imidacloprid to the ecological system could affect aquatic life as well as human. The toxic pollutants would affect the quality of agriculture run off, in turn contaminating water bodies acting as sink for these runoffs. Hence, there is need for reparation of these pollutants. Azospirillium biofertilizer and Rhizobium biofertilizer were used as adsorbent for the removal of imidacloprid. These biofertilizers have capability to reduce the harmful component as well as be useful for plant growth. Azospirillium bacteria and Rhizobium bacteria are competent for the removal of organic pollutant from wastewater. These biofertilizers maintain biological activity without any adverse effect. The adsorptive removal of imidacloprid by using Azospirillium biofertilizer and Rhizobium biofertilizer was investigated at different conditions using batch experimentation. Optimization of parameters, such as dosage, time, temperature, pH, and agitation speed, was carried out. Equilibrium adsorption was illustrated by Langmuir and Freundlich isotherms. The kinetic data was best described by intraparticle diffusion and pseudo-second-order model. Reusability study showed good removal efficiency of imidacloprid after fourth use also. The investigations show that these materials have potential to be an excellent alternative for removal of pesticides while supporting plant growth.

Mutation analysis and characterisation of F9 gene in haemophilia- B population of India

Sujayendra Kulkarni,Rajat Hegde,Smita Hegde,Suyamindra S. Kulkarni,Suresh Hanagvadi,Kusal K. Das,Sanjeev Kolagi,Pramod B. Gai,Rudragouda Bulagouda 대한혈액학회 2021 Blood Research Vol.56 No.4

Background Hemophilia B (HB) is an X-linked bleeding disorder resulting from coagulation factor IX defects. Over 3,000 pathogenic, HB-associated mutations in the F9 gene have been identified. We aimed to investigate the role of F9 variants in 150 HB patients using sequencing technology. Methods F9 gene sequences were amplified from peripheral blood-derived DNA and sequenced on an Applied Biosystems (ABI) 3500 Sanger sequencing platform. Functional and structural predictions of mutant FIX were analyzed. Results Among 150 HB patients, 102 (68%), 30 (20%), and 18 (12%) suffered from severe, moderate, and mild HB, respectively. Genetic analysis identified 16 mutations, including 3 novel mutations. Nine mutations (7 missense and 2 stop-gain) were found to be pathogenic. Only 3 mutations (c.127C>T, c.470G>A, and c.1070G>A) were associated with different severities. While 2 mutations were associated with mild HB cases (c.304C>T and c.580A>G), 2 (c.195G>A and c.1385A>G) and 3 mutations (c.223C>T, c.1187G>A, and c.1232G>A) resulted in moderate and severe disease, respectively. Additionally, 1 mutation each was associated with mild-moderate (c.*1110A>G) and mild-severe HB disease (c.197A>T), 4 mutations were associated with moderate-severe HB cases (c.314A>G, c.198A>T, c.676C>T, and c.1094C>A). FIX concentrations were lower in the mutated group (5.5±2.5% vs. 8.0±2.5%). Novel p.E66D and p.S365 mutations were predicted to be pathogenic based on changes in FIX structure and function. Conclusion Novel single nucleotide polymorphisms (SNPs) largely contributed to the pathogenesis of HB. Our study strongly suggests that population-based genetic screening will be particularly helpful to identify risk prediction and carrier detection tools for Indian HB patients.

Temporal Variation of Dominant Modes of Oscillations in the Wind Field in the Surface Boundary Layer over a Costal Indian Station

Makarand A. Kulkarni,J. R. Kulkarni,G. V. Rama,P. N. Sen 한국기상학회 2007 한국기상학회 학술대회 논문집 Vol.2007 No.-

Numerical and Experimental Analysis of Transpiration Cooling in Carbon Dioxide Atmosphere at Hypersonic Mach Numbers

V. N. Kulkarni,P. S. Kulkarni,K. P. J. Reddy 한국전산유체공학회 2003 한국전산유체공학회 학술대회논문집 Vol.2003 No.10

Hologram Quantitative Structure Activity Relationship Analysis of JNK Antagonists

Kulkarni, Seema A.,Madhavan, Thirumurthy The Basic Science Institute Chosun University 2015 조선자연과학논문집 Vol.8 No.2

c-Jun N-terminal kinase-3 (JNK3) is a member of the mitogen-activated protein kinase family (MAPK), and plays an important role in neurological disorders. Therefore, identification of selective JNK3 inhibitor may contribute towards neuroprotection therapies. In this work, we performed hologram quantitative structure-activity relationship (HQSAR) on a series of thiophene trisubstituted derivatives. The best predictions were obtained for HQSAR model with $q^2=0.628$ and $r^2=0.986$. Statistical parameters from the generated QSAR models indicated the data is well fitted and have high predictive ability. HQSAR result showed that atom, bond and chirality descriptors play an important role in JNK3 activity and also shows that electronegative groups is highly favourble to enhance the biological activity. Our results could be useful to design novel and selective JNK3 inhibitors.

Application of Docking Methods: An Effective In Silico Tool for Drug Design

Kulkarni, Seema,Madhavan, Thirumurthy The Basic Science Institute Chosun University 2013 조선자연과학논문집 Vol.6 No.2

Using computational approaches we can dock small molecules into the structures of Macromolecular targets and then score their potential complementarity to binding sites is widely used in hit identification and lead optimization techniques. This review seeks to provide the application of docking in structure-based drug design (binding mode prediction, Lead Identification and Lead optimization), and also discussed how to manage errors in docking methodology in order to overcome certain limitations of docking and scoring algorithm.

The label free DNA sensor using a silicon nanowire array

Kulkarni, A.,Xu, Y.,Ahn, C.,Amin, R.,Park, S.H.,Kim, T.,Lee, M. Elsevier Science Publishers 2012 Journal of biotechnology Vol.160 No.3

Biosensors based on silicon nanowire (Si-NW) promise highly sensitive dynamic label free electrical detection of various biological molecules. Here we report Si-NW array electronic devices that function as sensitive and selective detectors of as synthesized 2D DNA lattices with biotins. The Si-NW array was fabricated using top-down approach consists of 250 nanowires of 20μm in length, equally spaced with an interval of 3.2μm. Measurements of photoresistivity of the Si-NW array device with streptavidin (SA) attached on biotinylated DNA lattices at different concentration were observed and analyzed.. The conductivity in the DNA lattices with protein SA shows significant change in the photoresistivity of Si-NW array device. This Si-NW based DNA sensor would be one of very efficient devices for direct, label free DNA detection and could provide a pathway to immunological assays, DNA forensics and toxin detection in modern biotechnology.

The effect of denture base surface pretreatments on bond strengths of two long term resilient liners

Kulkarni, Rahul Shyamrao,Parkhedkar, Rambhau The Korean Academy of Prosthodonitics 2011 The Journal of Advanced Prosthodontics Vol.3 No.1

PURPOSE. Purpose of this study was to evaluate effect of two surface treatments, sandblasting and monomer treatment, on tensile bond strength between two long term resilient liners and poly (methyl methacrylate) denture base resin. MATERIALS AND METHODS. Two resilient liners Super-Soft and Molloplast-B were selected. Sixty acrylic resin (Trevalon) specimens with cross sectional area of $10{\times}10$ mm were prepared and divided into two groups of 30 specimens each. Each group was surface treated (n = 10) by sandblasting (250 ${\mu}$ alumina particles), monomer treatment (for 180 sec) and control (no surface treatment). Resilient liners were processed between 2 poly(methyl methacrylate) surfaces, in the dimensions of $10{\times}10{\times}3$ mm. Tensile strength was determined with Instron Universal testing machine, at a crosshead speed of 5 mm/min; and the modes of failure (adhesive, cohesive or mixed) were recorded. The data were analyzed using one-way ANOVA, followed by Tukey HSD test (${\alpha}$= 0.05). RESULTS. Monomer pretreatment of acrylic resin produced significantly higher bond strengths when compared to sandblasting and control for both resilient liners (P < .001). Sandblasting significantly decreased the bond strength for both the liners when compared to monomer pretreatment and control (P < .001). Mean bond strength of Super-Soft lined specimens was significantly higher than Molloplast-B in various surface treatment groups (P < .05). CONCLUSION. Surface pretreatment of the acrylic resin with monomer prior to resilient liner application is an effective method to increase bond strength between the base and soft liner. Sandblasting, on the contrary, is not recommended as it weakens the bond between the two.

The plastic optical fiber cantilever beam as a force sensor

Kulkarni, Atul,Na, Jeonggil,Kim, Youngjin,Kim, Taesung Wiley Subscription Services, Inc., A Wiley Company 2009 MICROWAVE AND OPTICAL TECHNOLOGY LETTERS Vol.51 No.4

<P>This study reports use of inexpensive intensity-based plastic optical fiber (POF) in the form of cantilever beam to monitor the force. The cantilever consists of POF surface bonded on the surface of metal beams viz. spring steel (SS) and mild steel (MS) and subjected to force. The performance of the force sensor is evaluated during macro-bending caused because of the deflection of the beam by applied force. Experimentally obtained detector output of POF, which could be measured with negligible hysteresis is compared with finite element analysis in the range between 0.0098 and 19.613 N. The reproducibility of the sensor is observed in the limit of ±1%. The proposed sensor can replace stain gauge load cell to certain extent in some applications. © 2009 Wiley Periodicals, Inc. Microwave Opt Technol Lett 51: 1020–1023, 2009; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/mop.24217</P>

Comparative genome analysis to identify SNPs associated with high oleic acid and elevated protein content in soybean

Kulkarni, Krishnanand P.,Patil, Gunvant,Valliyodan, Babu,Vuong, Tri D.,Shannon, J. Grover,Nguyen, Henry T.,Lee, Jeong-Dong,Belzile, F. National Research Council of Canada, Conseil natio 2018 Genome Vol. No.

<P> The objective of this study was to determine the genetic relationship between the oleic acid and protein content. The genotypes having high oleic acid and elevated protein (HOEP) content were crossed with five elite lines having normal oleic acid and average protein (NOAP) content. The selected accessions were grown at six environments in three different locations and phenotyped for protein, oil, and fatty acid components. The mean protein content of parents, HOEP, and NOAP lines was 34.6%, 38%, and 34.9%, respectively. The oleic acid concentration of parents, HOEP, and NOAP lines was 21.7%, 80.5%, and 20.8%, respectively. The HOEP plants carried both FAD2-1A (S117N) and FAD2-1B (P137R) mutant alleles contributing to the high oleic acid phenotype. Comparative genome analysis using whole-genome resequencing data identified six genes having single nucleotide polymorphism (SNP) significantly associated with the traits analyzed. A single SNP in the putative gene Glyma.10G275800 was associated with the elevated protein content, and palmitic, oleic, and linoleic acids. The genes from the marker intervals of previously identified QTL did not carry SNPs associated with protein content and fatty acid composition in the lines used in this study, indicating that all the genes except Glyma.10G278000 may be the new genes associated with the respective traits. </P>