한국 농촌거주 정상인의 Leptospira Interrogans에 대한 항체보유율 현황

최강원,최성배,국윤호,장우현,우준희,박경희,강재승,차창룡,김양수,김석용,윤종구,김익상,이우곤,최명식,송영욱 대한감염학회 1988 감염 Vol.20 No.3

It has been well known that leptospiroisis is a one of the prevailing acute febrile diseases in Korea. In order to find out the incidence of past infection of L. interrogans among normal population, a total of 1,171 sera obtained from rural residents and tested by microscopic agglutination test. All of normal resident's sera were collected during July in 1987, just before epidemic season, at Keochang county(248 sera), at Wanju county(145 sera), at Wonseoung county(255 sera), at Cholwon county(411 sera) and at Kongju county(112 sera). Seropositive rate against L. interrogans among rural residents was 12.0% and ranged from 2.7% in Kongju county to 18.5% in Cholwon county. There was a higher seropositive rate in males and the majority of seropositives occurred in the age group over 30 yesrs. Icterohaemorrhagiae was a most commonly reacting serotype of L. interrogans and followed by CH-48 and Canicola. The sera reacted with Cynopteri, Ballum, Australis, and Pyrogenes were present also.

혈청 LDH 효소분석 검사의 효준화에 관한 연구(Ⅴ)

이창규,이승관,조경진,박종성,정수경,유병서,박상숙,류정록,남현철,김석수,김상섭,김재영,이국성 高麗大學校 倂設 保健大學 保健科學硏究所 1998 保健科學論集 Vol.24 No.1

Since the Korean Association of Quality Assurance for Clinical Laboratory was established in 1976, the numbers of the participating hospitals nowadays were increased from 40 to 450 or more hospitals all over the country. In the early day the both internal and external quality control programs were started at the same time, however, the external QC programs prevailed over the internal ones putting the cart before the horse. The accuracy and the precision of the data are not satisfying in spite of the 20 years experiences of quality control programs. All the daily data should be reported after the validity is assured by the self-evaluation not by third party evaluation. It is regret that the CVs of enzymatic tests in external QC are exceeding 10% and there is no sign of diminishing CVs. One of the major reasons is that the types of anaytical instrurments and reagents are diverisified. Moreover, most of them are subject to the standards of the respective country of manufacturer. Accordingly, we can make a suggestion that the reagents and instruments should be imported or supplied meeting the established requirements for Korean standardization.

The influence of budA deletion on glucose metabolism related in 2,3-butanediol production by Klebsiella pneumoniae

Kim, B.,Lee, S.,Yang, J.,Jeong, D.,Shin, S.H.,Kook, J.H.,Yang, K.S.,Lee, J. IPC Science and Technology Press ; Elsevier Scienc 2015 Enzyme and microbial technology Vol.73 No.-

Klebsiella pneumoniae (K. pneumoniae), which is a promising microorganism for industrial bulk production of 2,3-butanediol (2,3-BDO), naturally converts glucose to 2,3-BDO. The 2,3-BDO biosynthesis from glucose is composed of three steps; α-acetolactate biosynthesis by α-acetolactate synthase (budB); acetoin biosynthesis by α-acetolactate decarboxylase (budA); and 2,3-BDO biosynthesis by acetoin reductase (budC). In an effort to understand the influence of blocked 2,3-BDO pathway on K. pneumoniae glucose metabolism by budA deletion, we constructed K. pneumoniaeΔwabGΔbudA (SGSB106). Carbon flux distribution analysis, transcriptome analysis and extracellular amino acid concentration analysis were carried out to understand the effects of the budA deletion, and K. pneumoniaeΔwabG (SGSB100) was used as a control strain. Approximately 50.3% decrease in CO<SUB>2</SUB> emission; and approximately 3.8-fold increase in amino acid production was observed in SGSB106. In addition to, among the amino acids, valine production significantly increased, suggesting that the branched-chain amino acid biosynthesis (BACC) in SGSB106 was activated by deletion of budA. Furthermore, whole genome transcriptome analysis of SGSB106 and SGSB100, correlates with the results from carbon distribution and amino acids concentration analyses.

마이크로 전기·화학 복합형상 제거시스템

이희원,국경훈,김기원,김태곤,유병한,정재원,한민섭,정영훈,민병권,이상조 한국공작기계학회 2005 한국공작기계학회 춘계학술대회논문집 Vol.2005 No.-

Microfactory is effective method for machining micro size component. Electro-chemical machining can be more suitable to a micro factory than other machining methods in terms of maintaining high accuracy. Surface profile of EDM Machined component is predicted by micro EDM simulation using super positioning spark crater. Planar motor and micro pump are developed to construct microfactory system.

Hemophilus Vaginalis 地域 菌株의 生物學的 性狀에 관한 硏究

鞠允鎬,李光浩,張友鉉 최신의학사 1980 最新醫學 Vol.23 No.7

죽초액을 급여한 돼지고기의 저장 중 육질특성 변화

국길,김광현 한국동물자원과학회 2003 한국축산학회지 Vol.45 No.2

본 연구는 평균체중 60.0±1.5㎏인 육성비육돈 30두를 대조구, 죽초액 2% 급여구 및 죽초액 4% 급여구의 3개 처리구로 10두씩 배치하여 4개월간 사양실험을 실시한 후 도축하여 등심육을 시료로 채취하여 저장 중 육질특성 변화를 알아보고자 실시하였다. pH는 저장 3일 이후 죽초액 2%와 4% 급여구가 대조구에 비해 유의적(P<0.05)으로 감소함을 나타냈다. 명도(L)는 저장 1일과 3일에 2%와 4% 급여구가 대조구에 비해 유의적(P<0.05)으로 높게 나타났다. 적색도는 저장 1일과 3일에 죽초액 4% 급여구가 대조구에 비해 유의적(P<0.05)으로 낮게 나타났다. 황색도는 저장기간 동안 죽초액 2%와 4% 급여구가 대조구에 비해 유의적(P<0.05)으로 높게 나타났다. 가열감량은 저장 3일 이후 죽초액 2%와 4%급여구가 대조구에 비해 유의적(P<0.05)으로 높게 나타났다. 전단력은 대조구에 비해 유의적(P<0.05)으로 낮게 나타나 연도 개선효과를 보였다. 지방산패도는 저장 6일과 9일에 죽초액 2%와 4% 급여구가 유의적(P<0.05)으로 감소하였다. 총균수는 죽초액 2%와 4% 급여구가 대조구에 유의적(P<0.05)으로 낮게 나타났다. 냄새는 저장 1일과 3일에 죽초액 2%와 4% 급여구가 대조구에 비해 유의적(P<0.05)으로 높은 점수를 받아 돼지 특유의 이취가 감소하는 효과를 나타냈다. 외관은 저장 1일에 죽초액 2%와 4% 급여구가 대조구에 비해 유의적(P<0.05)으로 증가함을 나타냈다. 맛은 저장 1일과 3일, 6일에 죽초액 2%와 4% 급여구가 대조구에 비해 유의적(P<0.05)으로 높게 나타났다. 이상이 결과를 종합해보면 죽초액 2%와 4% 급여된 돼지고기는 육질특성과 저장안정성이 개선된 것으로 사료된다. This experiment was conducted to investigate the changes in meat quality characteristics on refrigerated(4℃) pork loin fed with supplemental levels of Bamboo vinegar(BV). Thirty pigs were divided into 3 groups of 10 pigs. Dietary levels of Bamboo vinegar 0%(control), 2% and 4% were included in experimental diets of each of the groups. The pH value was decreased(P<0.05) in 2% and 4% BV compared to the control after 3d of storage. Lightness was increased(P<0.05) in 2% and 4% BV compared to the control at 1d and 3d. Redness was decreased(P<0.05) in 4% VB compared to the control at 1d and 3d. The 2% and 4% BV showed a significant(P<0.05) increase in yellowness throughout the whole experiment compared to control. Cooking loss was increased(P<0.05) in 2% and 4% BV compared to the control after 3d of storage. Shear force was decreased(P<0.05) in 2% and 4% BV compared to control at 1d and 3d. TBA was decreased(P<0.05) in 2% and 4% BV at 6d and 9d. Total microbial counts was decreased(P<0.05) in 2% and 4% BV compared to control. The 2% and 4% BV scored higher(P<0.05) in odor evaluation compared to the control at 1d and 3d. The 2% and 4% BV also scored higher(P<0.05) than the control in the evaluation of appearance at 1d. The taste was increased(P<0.05) in 2% and 4% BV compared to control at 1d, 3d, and 6d. These results showed that the addition of 2% or 4% BV improved the meat quality and storage characteristics of refrigerated pork.

무화과 발효물의 급여가 한우의 생산성, 혈액성상 및 육질에 미치는 영향

국길,김광현 한국동물자원과학회 2002 한국축산학회지 Vol.44 No.6

본 연구는 한우 수소 10두를 공시하여 전남 영암군의 지역특산물인 무화과 열매와 잎을 발효시킨 발효물을 10% 첨가하여 출하전 8개월간 급여하였을 때 생산성, 혈액성상, 도체성적 및 육질특성을 조사하였다. 한우 수소에 무화과 발효물 10%을 급여했을 때 사료섭취량은 무화과 발효물 급여구가 대조구에 비해 약간 증가하였으나, 종료체중과 일당증체량에서는 처리구간에 유의적인 차이는 나타내지 않았다. 혈액성상은 처리구간에 유의적인 차이는 없었다. 도체성적에서는 육량등급에는 처리간에 차이는 없었으나 육질등급에서 무화과 발효물 급여구에서 근내지방도가 유의적(P<0.05)으로 증가하여 육질등급 개선 효과가 있었다. 산육성적은 처리간에 유의적인 차이가 없었다. 등심의 이화학적 특성으로 조지방 함량이 무화과 발효물 급여구에서 유의적(P<0.05)으로 증가하였다. 물리적 특성에서는 pH와 육색, 가열감량에는 처리간에 유의적인 차이는 없었으나, 전단력과 cholesterol 함량은 무화과 발효물 급여구에서 유의적(P<0.05)으로 감소하였다. 지방산 조성에서는 등심부위에서 리놀레인산이 무화과 발효물 급여구에서 유의적(P<0.05)으로 증가하였으며 무화과 발효물 급여구의 등심부위 피하지방에서 C16:0이 유의적(P<0.05)으로 감소한 반면 C18:1는 유의적(P<0.05)으로 증가하였다. 따라서 무화과 발효물 급여구에서 포화지방산이 유의적(P<0.05)으로 감소한 반면에 불포화지방산은 유의적(P<0.05)으로 증가하였다. 관능평가에서는 냄새는 무화과 발효물 급여구에서 약간 개선되었으며 외관과 맛에 있어서는 무화과 발효물 급여구에서 유의적(P<0.05)으로 증가하였다. 이상의 결과, 무화과 열매와 잎을 발효시킨 발효물을 한우 수소에 급여시 도체등급 향상에 따른 축산농가의 생산성 향상과 더불어 한우고기의 연도개선과 저콜레스테롤 그리고 외관과 맛에 차별화로 고급육생산에 효과가 있는 것으로 사료된다. We investigated the effect of fig fermented product(FFP) supplementation on growth performance, serum profile, carcass performance, meat performance and meat quality in 10 bulls of Korean cattle. Concentrates diet was supplemented with substrate fermented from fig fruit and leaves at 10% of the diet. The feed intake of FFP were slightly higher than the control, but the final weight showed no sifnificant difference between the two. Daily weight gain and feed intake were increased in FFP. The serum profile had no significant difference in the treatment. In carcass performance, the meat quantity grade of the treatment had no significant difference, but in meat quality grade the marbling score of FFP was significantly(P<0.05) increased therefore it showed a positive effect on meat quality grade. Also there was no significant(P<0.05) difference of meat cut performance in the treatment. Due to the proximate characteristics of longissimuss muscles the crude fat content of the FFP was significantly(P<0.05) increased. There was no significant difference(P<0.05) in physical characteristics ; pH level, meat color and heat loss of the treatment, but the shear force value and the cholesterol content of FFP significantly(P<0.05) decreased. Crude fat was increased(P<0.05) and cooking loss, shear force and cholesterol concentration were decreased. In fatty acids composition of the FFP, the linoleic acid from the longissimus increased significantly(P<0.05). In subcataneous fat of longissimus of the FFP, C16:0(palmitic acid) significantly(P<0.05) decreased, but C18:1 significantly(P<0.05) increased. Therefore in FFP, the concentration of saturated fatty acid significantly decreased (P<0.05), but on the other hand the concentration of unsaturated fatty acids significantly (P<0.05) increased. In sensory evalution of the FFP, the evaluation of odor increased slightly in a positive manner, also the appearance and the taste increased significantly(P<0.05). In conclusion when annexing additional fig fermented product to Hanwoo bulls, the carcass grade improves and the livestock production increases. Also the shear force, lower cholesterol, improved appearance and taste will open the doors to high quality meat production.

죽초액의 첨가수준이 비육 한우암소의 육생산성, 혈액성상 및 육질에 미치는 영향

국길,김광현 한국동물자원과학회 2003 한국축산학회지 Vol.45 No.1

본 시험은 한우 암소에 대한 죽초액의 급여효과를 구명하기 위하여 비육중인 암소 15두를 대상으로 대조구와 죽초액 3% 및 6% 급여구의 3개 처리구에 5두씩 공시하여 4개월간 사양시험을 실시한 결과는 요약하면 다음과 같다. 일당증체량은 죽초액 3% 급여구에서 약간 증가한 반면에 죽초액 6% 급여구에서는 감소하였다. 사료섭취량은 죽초액 6% 급여구에서 유의적(P<0.05)으로 감소하였다. 혈액성상에서 glucose 농도는 죽초액 3% 및 6% 급여구에서 유의적(P<0.05)으로 감소하였다. Total protein과 cholesterol 농도는 죽초액 3% 및 6% 급여구가 유의적(P<0.05)으로 증가하였다. BUN의 농도는 죽초액 3% 급여구에서 유의적(P<0.05)으로 증가하였다. 도체성적에서 등심단면적은 유의적인 차이가 없었으나 등지방 두께가 죽초액 6% 급여구에서 유의적(P<0.05)으로 감소하였다. 근내지방도는 죽초액 3% 급여구에서 유의적(P<0.05)으로 높게 나타나 육질등급이 향상되었다. 죽초액 급여에 의한 부분육 성적에서 별다른 변화가 없었다. 등심의 이화학적 특성에서 조지방 함량이 죽초액 3% 급여구에서 유의적(P<0.05)으로 높게 나타났다. 전단력가와 콜레스테롤 함량은 죽초액 3% 및 6% 급여구에서 유의적(P<0.05)으로 감소하였다. 등심의 지방산 조성에서 16:0은 죽초액 3% 급여구에서 유의적(P<0.05)으로 감소한 반면에 18:1은 죽초액 3% 및 6% 급여구에서 유의적(P<0.05)으로 증가하였다. 따라서 죽초액 3% 급여구에서 포화지방산이 유의적(P<0.05)으로 감소한 반면에 불포화지방산이 유의적(P<0.05)으로 증가하였다. 관능평가에서 죽초액 급여에 의한 냄새와 외관의 개선효과는 없었다. 맛은 죽초액 3%와 6% 급여구가 대조구에 비해 유의적(P<0.05)으로 개선되는 효과를 나타냈다. 이상의 결과를 종합해 보면 한우 암소에 대한 죽초액 3% 급여는 근내지방도 및 조지방 함량의 증가, 전단력가 및 콜레스테롤 함량의 감소, 불포화지방산 비율의 증가, 그리고 관능평가에서 맛에 개선효과를 나타냈다. Effects of supplemental levels of Bomboo vinegar(BV)on growth performance, serum profile and meat quality in 15 Korean native Hanwoo cows was investigated. Concentrate diet was supplemented with Bamboo vinegar with 3%and 6% of the diet. Daily weight gain was increased slightly at 3% BV, but it was decreased in 6% BV. Fee intake was decreased(P<0.05) at 6% BV. Glucose concentration of serum profile was decreased(P<0.05) at 3% and 6% BV. Total protein and cholesterol concentrations were increased(P<0.05) at 3% and 6% BV. BUN concentration was increased(P<0.05) at 3% BV. In carcass characteristics the longissimus muscles of all BV treatments showed no significant(P<0.05) effects, but back fat thickness was decreased significantly(P<0.05) in 6% BV. Marbling score was increased significantly(P<0.05) at 3% BV, thus improving the meat quality. Cut meat production was not difference by treatment. Crude fat content of proximate chemical composition in longissimus muscle was increased(P<0.05) in 3% BV. Shear force and cholesterol contents were decreased(P<0.05) in 3% and 6% BV. The 16:0 of fatty acid composition in longissimus was decreased(P<0.05) whereas 18:1 was increased(P<0.05) at 3% and 6% BV. The composition of saturated fatty acids(SFA) was decreased(P<0.05), whereas unsaturated fatty acids(USFA) was increased(P<0.05) in 3% BV. Odor and appearance of sensory evaluation were not difference by treatment. Taste was improved significantly (P<0.05) in 3% and 6% BV with the peculiar and savory taste of Hanwoo being more emphasized. The results of this experiment indicated that 3% BV improved the marbling score and crude fat content, decreased the shear force and cholesterol contents, increased the USFA composition. and improved the taste of sensory evaluation in Korean native Hanwoo cows.

항균제 내성 대장균의 생태적 분포 Ⅲ. : Rifampicin, nalidixic acid, oxolinic acid와 sulfa 제제에 관하여

이광호,차창룡,국윤호,최명식,김익상,장우현,이승훈,신정식 대한화학요법학회 1983 대한화학요법학회지 Vol.1 No.1

We studied the differences in the MIC distribution of E. coli isolates, from a doctorless remote arwqwang Won. Do province+WD), a leprosarium (So Rock Do=SRD) and Seoul National University Gospital (SNUH> against rifampicin. nalidixic acid, oxolinic acid &a sulfa drags including co-trinoxawle. . So the widespread and prolonged- use of antimicrobial agents is presumed to facilitate the increase of drng-resistapt strains among the normal flora of the residents in a comunity that .the isolates from SNUH- were further divided into two groups, SNUH in-patient (IP) and SNUH out-patient(OP) and the isolates f p m SRD into DDS (isolates. from.leprosy patients.who are taking DDS only) and R f (isolates from leprosy patients who are taking rifampicin-.and DQS) grougs. Nnmber of, E. coli isolates studied were 93 st;ains from SNUH patients (SNUH-IP, 46; SNUF-OP, 43, 150 strains from SRD patients (SRD-DDS, 102; SRD-Rf,' 48) and 119 strains from 'KWD residents. DisLribntion of E. mli- strains a.mording to thir respective MTC's shows that E. coli possesses not only an intrinsic component in resistance (or susceptibility) to the drugs (rif-ampicin, 2-32 pg/ml; nafii%Xic a,cid, 1-16 pg/d; oxolinic acid, * 0.13-2 pg/ml; DDS, 32-1024 pg/ml; sulfadiazine, 2-256 pg/d, sulfamera&& 4-256 pg/ml; sulfadimethoxine, .16~1024 pg/ml; sdfamonornethoxine, ~-12~,pg&d; sulfamethoxazde, 2-128 pg/m&. trimetk0p-Irim, 0.06-2 pg/fnl; co-trimoxwole, 0,. 25-4 pg/ml) but also distinct MIC values of acquired resistance quite different from the intrinsic ones. The .carriage rate of rifampicin resistant strains in the SRD-Rf patients was the higheest among the study:groups while t m e , of SNUH patients and of the KWD residents were equally negligible, as expectediy. Drug-resistant E. coli strains against nalidixic acid and oxolinic acid were uncommon i n the SNUH, SRD and KWD isolates. However, resistance to sulfa drugs were prevalent in the SNUH, SRD and KWD isolates in the decreasing order. Taken together, these results strongly suggest that drug resistznce in Korea is a localized problem in some community under a high selection pressure of antimicrobial agents rather than a nation-wide one. Trimethoprim resistance gave rise to the high-level resistance 'to co-trimoxazde while the sulfamethoxazole resistance t o the low level resistance against the co-trimoxazole.

Structure-Activity Relationships of Truncated D- and L-4´-Thioadenosine Derivatives as Species-Independent A₃Adenosine Receptor Antagonists¹

Jeong, Lak Shin,Shantanu Pal,Choe, Seung Ah,Choi, Won Jun,Kenneth A. Jacobson,Zhan-Guo Gao,Athena M. Klutz,Xiyan Hou,Kim, Hea Ok,Lee, Hyuk Woo,Lee, Sang Kook,Dilip K. Tosh,Moon, Hyung Ryong 이화여자대학교 약학연구소 2009 藥學硏究論文集 Vol.- No.19

Novel D- and L-4´-thioadenosine derivatives lacking the 4´-hydroxymethyl moiety were synthesized, starting from D-mannose and D-gulonic γ-lactone, respectively, as potent and selective species-independent A₃ adenosine receptor (AR) antagonists. Among the novel 4´-truncated 2-H nucleosides tested, a N^(6)-(3-chlorobenzyl) derivative 7c was the most potent at the human A₃ AR (K_(i) = 1.5 nM), but a N^(6)-(3-bromobenzyl) derivative 7d showed the optimal species-independent binding affinity.