Discovery of urinary metabolomic biomarkers for early detection of acute kidney injury

Won, A Jin,Kim, Siwon,Kim, Yoon Gyoon,Kim, Kyu-Bong,Choi, Wahn Soo,Kacew, Sam,Kim, Kyeong Seok,Jung, Jee H.,Lee, Byung Mu,Kim, Suhkmann,Kim, Hyung Sik The Royal Society of Chemistry 2016 Molecular bioSystems Vol.12 No.1

<P>The discovery of new biomarkers for early detection of drug-induced acute kidney injury (AKI) is clinically important. In this study, sensitive metabolomic biomarkers identified in the urine of rats were used to detect cisplatin-induced AKI. Cisplatin (10 mg kg(-1), i.p.) was administered to Sprague-Dawley rats, which were subsequently euthanized after 1, 3 or 5 days. In cisplatin-treated rats, mild histopathological alterations were noted at day 1, and these changes were severe at days 3 and 5. Blood urea nitrogen (BUN) and serum creatinine (SCr) levels were significantly increased at days 3 and 5. The levels of new urinary protein-based biomarkers, including kidney injury molecule-1 (KIM-1), glutathione S-transferase-alpha (GST-alpha), tissue inhibitor of metalloproteinase-1 (TIMP-1), vascular endothelial growth factor (VEGF), calbindin, clusterin, neutrophil, neutrophil gelatinase-associated lipocalin (NGAL), and osteopontin, were significantly elevated at days 3 and 5. Among urinary metabolites, trigonelline and 3-indoxylsulfate (3-IS) levels were significantly decreased in urine collected from cisplatin-treated rats prior to histological kidney damage. However, carbon tetrachloride (CCl4), a hepatotoxicant, did not affect these urinary biomarkers. Trigonelline is closely associated with GSH depletion and results in insufficient antioxidant capacity against cisplatin-induced AKI. The predominant cisplatin-induced AKI marker appeared to be reduced in urinary 3-IS levels. Because 3-IS is predominantly excreted via active secretion in proximal tubules, a decrease is indicative of tubular damage. Further, urinary excretion of 3-IS levels was markedly reduced in patients with AKI compared to normal subjects. The area under the curve receiver operating characteristics (AUC-ROC) for 3-IS was higher than for SCr, BUN, lactate dehydrogenase (LDH), total protein, and glucose. Therefore, low urinary or high serum 3-IS levels may be more useful for early detection of AKI than conventional biomarkers.</P>

국민건강보험의 의약품 가격전략: 산업과 보험의 공존

김원식 ( Won Shik Kim ),이규식 ( Kyu Sik Lee ) 한국사회보장학회 2008 社會 保障 硏究 Vol.24 No.4

Antiadhesive effect and safety of oxidized regenerated cellulose after thyroidectomy

Kyoung Sik Park,Kyu Eun Lee,Do Hoon Ku,Su-Jin Kim,Won Seo Park2,Hoon Yub Kim3,,Mi Ra Kwon1,Yeo-Kyu Youn1 대한외과학회 2013 Annals of Surgical Treatment and Research(ASRT) Vol.84 No.6

Purpose: To evaluate the antiadhesive effects and safety of an oxidized regenerated cellulose (Interceed) after thyroidectomy. Methods: Seventy-six thyroidectomized patients were prospectively randomized into two groups with regard to the use of Interceed. We evaluated each group for their adhesive symptoms using four subjective and four objective items at the 2nd week, 3rd and 6th month after thyroidectomy. All patients were examined for vocal cord motility by indirect laryngoscope at each period. Results: Total adhesion scores at each postoperative follow-up period decreased with time, but were not significantly different in each group. The median score for swallowing discomfort for liquid was significantly lower in the Interceed group than in the control group 2 weeks after surgery. In addition, the severity of skin adhesion to the trachea was reduced in the Interceed group compared with the control group 6 months after surgery. During the study, there were no adverse effects or significant differences in postoperative complications between the groups. Conclusion: Interceed appeared to be safe and effective in improving neck discomfort at early postoperative periods and preventing skin adhesion to the trachea 6 months after thyroidectomy.

데이터 마이닝을 활용한 한국 프로야구 구단의 승패예측과 승률 향상을 위한 전략 도출 연구

김원종 ( Kim Won-jong ),최연식 ( Choi Yeon-sik ),유동희 ( Yoo Dong-hee ) 한국스포츠산업경영학회 2018 한국스포츠산업경영학회지 Vol.23 No.3

본 연구에서는 데이터 마이닝 기법을 활용하여 한국 프로야구의 승패예측모형을 구축하는 실험을 진행하였다. 이를 위해, 2017년에 실시된 한국 프로야구 10개 구단의 전체 경기 중 무승부 경기를 제외한 1,418경기에 대한 자료를 사용하였다. 승패예측모형에는 의사결정나무, 베이즈넷, 인공신경망 알고리즘과 앙상블 기법인 배깅과 부스팅이 사용되었으며, 그 결과 배깅 기법에 인공신경망을 적용한 예측모형에서 가장 높은 예측률인 85.18%를 기록하였다. 다음으로 의사결정나무 기반 예측모형을 활용하여 한국 프로야구 전체 구단에 관한 8개의 승패규칙을 도출하였다. 여기에서 승패규칙은 승패예측에 영향을 미치는 주요 요인들인 팀출루율, 팀타율, 피안타, 안타, 타석, 타수로 표현되며, 도출된 규칙을 바탕으로 구단의 승률 향상에 도움을 주는 전략을 제안하였다. 또한 플레이오프 진출 구단과 미진출 구단에 관한 승리규칙을 각각 4개씩 도출하였고 이를 바탕으로 두 집단에 맞춤화된 승률 향상 전략을 제시하였으며, 실제 구단에서 선수를 영입한 방향과의 비교를 통해 제시된 전략의 활용 가능성을 확인하였다. This study conducted an experiment to develop win-loss prediction models for the Korean professional baseball league using data mining techniques. To this end, we used data on 1,418 games from all games played by the ten Korean professional baseball teams in 2017, except draw games. We developed win-loss prediction models using not only a decision tree, Bayse net, and artificial neural network algorithms, but also ensemble methods, such as bagging and boosting. As a result, first, we found that the artificial neural network-based prediction model using the bagging method reported the best accuracy (85.18%). Second, we derived eight win-loss rules for entire teams from the decision tree-based prediction model. These rules consist of six influential factors: team on base average, team batting average, hit by opponent, hit, plate appearances, and at bat. Using the derived rules, we proposed helpful strategies for improving the winning rate. Third, we derived four winning rules for both playoff teams and non-playoff teams; using the rules we proposed customized strategies for improving the winning rate of the two different groups. Finally, we confirmed the feasibility of the proposed strategies by comparing non-playoff teams’ actual player recruitment strategies.

Metagenomic Analysis of Fungal Communities Inhabiting the Fairy Ring Zone of Tricholoma matsutake

( Miae Kim1 ),( Hyeok Jun Yoon ),( Young Hyun You ),( Ye Eun Kim ),( Ju Ri Woo ),( Yeong Gyo Seo ),( Gyeong Min Lee ),( Young Ja Kim ),( Won Sik Kong ),( Jong Guk Kim ) 한국미생물 · 생명공학회 2013 Journal of microbiology and biotechnology Vol.23 No.10

Tricholoma matsutake, an ectomycorrhiza that has mutual relationships with the rootlet of Pinus denisflora, forms a fruiting body that serves as a valuable food in Asia. However, the artificial culture of this fungus has not been successful. Soil fungi, including T. matsutake, coexist with many other microorganisms and plants; therefore, complex microbial communities have an influence on the fruiting body formation of T. matsutake. Here, we report on the structures of fungal communities associated with the fairy ring of T. matsutake through the pyrosequencing method. Soil samples were collected inside the fairy ring zone, in the fairy ring zone, and outside the fairy ring zone. A total of 37,125 sequencing reads were obtained and 728 to 1,962 operational taxonomic units (OTUs) were observed in the sampling zones. The fairy ring zone had the lowest OTUs and the lowest fungal diversity of all sampling zones. The number of OTUs and fungal taxa inside and outside the fairy ring zone was, respectively, about 2 times and 1.5 times higher than the fairy ring. Taxonomic analysis showed that each sampling zone has different fungal communities. In particular, out of 209 genera total, 6 genera in the fairy ring zone, such as Hemimycena, were uniquely present and 31 genera, such as Mycena, Boletopsis, and Repetophragma, were specifically absent. The results of metagenomic analysis based on the pyrosequencing indicate a decrease of fungal communities in the fairy ring zone and changes of fungal communities depending on the fairy ring growth of T. matsutake.

Presurgical Naso-Alveolar Molding Appliance for Unilateral Cleft Lip and Palate

Baek, Seung-Hak,Yang, Won-Sik,Kim, Sukwha 대한치과교정학회 1998 대한치과교정학회지 Vol.28 No.6

본 연구의 목적은 1)편측성 순구개열 신생아 환자에 대한 술전 비치조 정형장치(PNAM)치료를 소개하고 2) 이 장치의 치조골 정형 효과를 평가하는 것이다. 서울대학교 병원 교정과에 내원하여 PNAM장치를 사용하여 치료받은 4명의 편측성 순구개열 호나자(남자 3명, 여자 1명, 평균 초진연령: 23.2일)을 대상으로 하였으며, 치조골에서 대분절과 소분절 파열부간의 평균거리는 8.27mm였고 치조골 정형치료의 평균기간은 9.7주였다. 초진시(Ti)와 성공적인 치조골 정형술이 이루어진 시기(T2)에 이들 환자의 인상을 채득하여 모형을 제작한 후, 사진촬영과 digital caliper를 사용하여 7개의 길이 항목과 5개의 각도항목을 계측하였다. SPSS win. ver 7.5프로그램을 사용하여 통계처리 하였고, 평균치의 차이를 비교하기 위하여 paired t-test를 사용하였다. 1. 신생아 치조골 후방부는 치조골 정형술을 시행하는 동안 안정된 구조물이었다. 2. 치조골 정형술에 의하여 대분절의 전방 성장이 억제되었다. 3. 치조골 정형술에 의한 파열부의 패쇄는 주로 대분절 전방부의 내측과 후방 굴곡 및 소분절 전체의 외측 굴곡에 의한 것이었다. The goals of this study were to present presurgical naso-alveolar molding(PNAM) appliance in unilaterla cleft lip and palate treatment and to evaluate the effects of PNAM appliance on alveolar molding. Samples were consisted of 4 unilateral cleft lip and palate infants(3 males and 1 female, mean age=23.2 days after birth) who were treated with PNAM appliances in Department of Orthodontics, Seoul National University Dental Hospital. Average alveolar cleft gap between the greater and lesser segment was 8.27 mm and average duration of alveolar molding treatment was 9.7 weeks. These patients' models were obtained at initial visit(T1) and after successful alveolar molding(T2). Seven linear and five angular variables were measured by using photometry and digital caliper. All statistical analyses were performed by SPSS win ver. 7.5 program. Paired t-test was used to compare the mean values. 1. The posterior part of alveolar segments are the stable structures during alveolar molding treatment period in infants. 2. Forward growth of th greater segment may be hindered by the action of alveolar molding. 3. The closure of cleft gap during alveolar molding were usually due to inward and backward bending of the anterior part of the greater segment and outward bending of the whole lesser segment.

물리치료(학)과 학생들이 지각한 물리치료사의 이미지와 자아존중감과의 관련성 분석

김은애 ( Eun Ae Kim ),이건철 ( Geon Cheol Lee ),배원식 ( Won Sik Bae ),김지혁 ( Chi Hyok Kim ) 대한물리의학회 2013 대한물리의학회지 Vol.8 No.1

PURPOSE: The purpose of this study is to look into relationship between image of physiotherapist and self-esteem of physiotherapy students and desirable image of physiotherapists offer to establish basic materials. METHODS: The subjects of this study are consist of 603 students of department of Physical therapy university and college in Busan and Kim-hae. Data are collected from June 9 to July 9 in 2011 by structured questionnaires. RESULTS: The result are as follows. 1. at the physiotherapist image score, the highest score question is Physiotherapist is specialist(3.45) and the lowest score question is Physiotherapist is treated samely with doctor(2.15). 2. at the self-esteem score, the highest score question is I think that I am at least as valuable as others(3.37) and the lowest score question is I can be poorly sometimes(2.81). 3. at the static results of physiotherapist image score gap by general characteristics, there are significantly different in gender(t=2.27, p=.02), similar profession distinction(t=4.30, p=.00), major satisfaction(t=5.74, p=.00), school system (t=-2.54, p=.01). 4. at the static results of self-esteem score gap by general characteristics, there are significantly different in years (f=5.14, p=.00), gender(t=4.09, p=.00), religion(f=2.87, p=.04), similar profession distinction(t=3.97, p=.00), major satisfaction(t=4.26, p=.00). 5. at the static results of good reputation of physiotherapists increase self-esteem of physiotherapy students score question are significantly different(r=0.53, p=.00). CONCLUSION: Accordingly, these results of this study that there is a very correlation between image of physiotherapist and self-esteem of physiotherapy students. Therefore, good reputation of physiotherapists increase self-esteem of physiotherapy students.

조현병 환자의 언어성 환청과 정신병리의 PANSS 요인들 간의다차원적 관계

신샘이,김세현,이남영,윤탁,김용식,정인원,Shin, Sam Yi,Kim, Se Hyun,Lee, Nam Young,Youn, Tak,Kim, Yong Sik,Chung, In Won 대한생물정신의학회 2015 생물정신의학 Vol.22 No.4

Objectives This study was aimed to examine the multidimensional relationship between auditory verbal hallucinations (AVHs) and Positive and Negative Syndrome Scale (PANSS) factors of psychopathology in the patients with schizophrenia. And we explored the differences between assessments to hallucination by the clinicians and patients. Methods 82 patients with schizophrenia who were assessed by the Hamilton Program for Schizophrenia Voices Questionnaire (HPSVQ), Psychotic Symptom Rating Scale-Auditory Hallucination (PSYRATS-AHS), and the PANSS were recruited. Hwang's five-factor model of PANSS, items and total scores of hallucination scales, Kim's and Haddock's factor models of hallucination were applied to examine the correlations between psychopathology and AVHs. AVH-positive patients was 50 in PANSS-HPSVQ group and 24 in PANSS-PSYRATS-AHS. These two groups were separately analyzed. Results Among the five factors of the PANSS, negative and depression/anxiety factors were correlated with the total scores of HPSVQ and PSYRATS-AHS, and positive and autistic preoccupation factors were correlated only with the total score of PSYRATS-AHS. The activation factor was correlated with none of the total scores of HPSVQ/PSYRATS-AHS. These correlation patterns of a total score of HPSVQ/PSYRATS-AHS were same in the emotional factor of HPSVQ and physical factor of PSYRATS-AHS respectively. In the items which showed significant correlations, correlation coefficients of PANSS-PSYRATS-AHS group ranged between 0.406-0.755 and those of PANSS-HPSVQ ranged between 0.283-0.420. Conclusions This study suggested that the psychopathological domains of schizophrenia were differentially correlated with AVHs and the assessment of AVHs by clinicians and patients showed substantial differences which should be integrated into the therapeutic interventions.

2010 해남, 평창, 원주지역 갈대 등 야초류의 이용실태와 사료가치 평가

서성(Sung Seo),한대덕(Dae Duk Han),장선식(Sun Sik Jang),김원호(Won Ho Kim),정민웅(Min Woong Jung),최진혁(Jin Hyuck Choi),김진숙(Jin Sook Kim),김하영(Ha Young Kim),이종경(Joung Kyong Lee) 한국초지조사료학회 2012 한국초지조사료학회지 Vol.32 No.1

본 연구는 부존 조사료자원 이용으로 수입사료를 줄이고 사료비를 절감하고자 2010년도에 전남 해남(간척지), 강원 평창(강변), 원주(섬강변), 전남 영광(와탄강변) 등 4개 지역에서 갈대와 억새 등 야초 자생지를 탐색하고, 수확·이용실태 조사와 함께 사료가치를 분석하였다. 전남 해남 해남목장 내 갈대(Phragmites communis) 위주의 야초 자생지는 300 ha로 예년에는 2회 곤포작업하여 연간 사일리지로 2,300~2,500톤(ha당 8톤)을 생산하였으나 2010년도는 1회 곤포조제로 사일리지 2,000톤(ha당 7톤)을 생산하였다. 유통가격은 롤 당 53천원으로 ㎏당 110원이었다. 곤포는 전량 자가소비하며, 외부 작업단에 롤 당 26천원의 작업비를 지불하였다. 연간 조수입은 2억 2,260만원이며, 소득은 1억원 수준(345천원/ha)이었다. 초장 150 ㎝의 갈대는 조단백질 8.4%, 상대사료가치 71.9 (건초 5등급), 건물 소화율 60.1%로 사료가치는 볏짚에 비해 약간 나은 것으로 평가되었다. 갈대 사일리지의 사료가치와 유기산 함량은 동일 지역에서 동일한 재료로 사일리지를 조제하더라도 수확시기에 따라 차이가 컸으며(CP 4.7~6.4%, RFV 62.2~78.9, 건물 소화율 41.9~53.9%), 갈대 사일리지의 품질은 수단그라스에 비해 불량하였다. 강원 평창 강변 자생갈대의 사료가치는 초장이 79, 117, 121, 142㎝로 생육이 진행됨에 따라 낮아져, 조단백질은 각각 13.9%에서 7.6%로, 상대사료가치는 90.7 (건초 3등급)에서 76.1 (건초 4등급)로, 건물 소화율은 72.9%에서 54.7%로 감소하였다. 원주 섬강 주변 야초류는 곤포수확 작업에 어려움이 있었으며, 초장 130 cm의 갈대는 조단백질 8.5%, 상대사료가치 82.3 (건초 4등급), 건물 소화율 70.2%로 사료가치는 높았으며, 220㎝의 성숙한 갈대는 조단백질 10.2%, 상대사료가치 65.1 (건초 5등급), 건물 소화율 48.9%로 볏짚 대비 조금 나은 것으로 평가되었다. 전남영광지역에서는 와탄천 주변에 자생하는 갈대를 수거 이용하고자 시도하였으나 경제성이 없어 중단하였다. 이상의 결과를 종합하여볼 때, 갈대, 억새 등 야초류는 현 상태에서 최대한 수거·이용하되, 사료가치를 유지하고 가축 기호성이 양호한 시기에 수확하는 것이 중요하며, 최소한 볏짚과 비슷하거나 그 이상의 사료가치를 기대하기 위해서는 7월 중순 이전(늦어도 7월까지)에 수확·이용하는 것이 권장된다. This study was carried out in 2010 to investigate the utilization and forage quality of native grasses, such as Phragmites communis which might reduce the cost of feeding domestic cattle. The regions surveyed were the Haenam ranch in Haenam, Pyeongchang, Wonju, and Yeonggwang. In Haenam, yearly silage production harvested from 300 ha was 2,000 MT (7 MT/ha). All of those round bale silages were self-consumed in that region, and marketing price was 50,000 55,000 won per roll (110 won/㎏). Phragmites communis of 150 ㎝ in length contained 8.4% crude protein (CP) with relative feed value (RFV) 71.9 and 60.1% in vitro dry matter digestibility (IVDMD), which was somewhat more favorable than forage quality of rice straw. The silage quality of Phragmites communis varied greatly by the time of harvest (CP 4.7 6.4%, RFV 62.2 78.9, and IVDMD 41.9 53.9%), even with the same district and of the same original forage materials. Generally, the quality of Phragmites communis silage of was poorer than that of sorghum * sudangrass hybrid. In Pyeongchang, forage quality of Phragmites communis was decreased from 13.9% to 7.6% in CP, 90.7 to 76.1 in RFV, and 72.9% to 54.7% in IVDMD, as plant was getting mature, from 79 ㎝ to 117 ㎝, 121 ㎝ or to 142 ㎝ in length. In Wonju, the quality values of Phragmites communis of 130 ㎝ in length were 8.5% CP, 82.3 RFV and 70.2% IVDMD, while those of matured grasses of 220 ㎝ in length were lower (10.2% CP, 65.1 RFV and 48.9% IVDMD), but this was a little more favorable than quality of rice straw. In Yeonggwang, feeding Phragmites communis was tried in a Hanwoo feed, but stopped due to low profitability. In conclusion, the overall quality of most native grasses including Phragmites communis in this survey was poor. Therefore, we recommend that Phragmites communis and native grasses should be harvested on June or July to obtain richer forage quality in forage values than rice straw.

온수배관을 이용한 온실의 난방성능

윤용철(Yong Cheol Yoon),신익수(Yik Soo Shin),김현태(Hyeon Tae Kim),배승범(Seoung Beom Bae),최진식(Jin Sik Choi),서원명(Won Myung Suh) (사)한국생물환경조절학회 2012 생물환경조절학회지 Vol.21 No.2

본 연구는 온실의 난방 에너지 절감을 목적으로 온실 내부에 알루미늄 온수배관을 설치하여 난방효과에 대한 기초자료를 구축하고자 수행되었다. 그 연구결과를 요약하면 다음과 같다. 전체 실험을 포함하여 온실내의 높이별 온도편차는 4.0~7.0℃ 정도의 범위로서 그 차이가 크게 나타났다. 팬코일유니트(FCU)를 작동시킨 경우가 작동시키지 않은 경우에 비해 유출입수의 온도차가 3.3℃ 정도 크고, 소비전력량은 36.2~40.1% 정도 증가하였으며, 시간당 방열량은 44.6~52.0% 정도 증가하는 것으로 나타났다. 실험기간동안 순환유량은 0.48~0.49L · s<SUP>?1</SUP> 정도의 범위에 있었고, 평균유속은 1.53~1.56m · s<SUP>?1</SUP> 정도였다. 유출입수의 평균 온도차는 6.24~11.50℃이었다. 최저 외기온 ?14.0~?0.6℃ 범위에서 설정온도별 방열량은 135,930~307,150㎉ 정도의 범위로서 시간당 9,610~19,630㎉ · h<SUP>?1</SUP> 정도의 범위에 있었다. 이것은 최대난방부하의 약 23~53% 정도의 난방에너지를 공급할 수 있을 것으로 나타났다. 전체 방열량과 소비전력량은 각각 2,548,306㎉ 및 3,075.7㎾h이다. 화석연료인 경유로 난방할 경우, 소요되는 경유의 총 소비량은 281.6L 정도이고 비용은 321,000won인 것으로 나타났다. 농가용 전력요금을 적용하면 전력사용에 대한 총비용은 110,730won 정도로서 경유 소비 비용의 33.5% 정도로 나타났다. 실험구의 온도가 대조구보다 약 8.3~14.6℃ 정도 높게 나타났다. This research was conducted to obtain basic data with regard to the heating performance that would be produced by installing an aluminum hot water pipe inside the greenhouse with the goal of reducing the heating energy in greenhouse. The research results are summarized as follows. The degree of difference in relation to the temperature by height within the greenhouse during the entire experiment was significant - within the range of 4.0~7.0℃. The temperature difference between incoming and outgoing water was about 3.3℃ greater when FCU was activated compared to when it was not activated. Meanwhile, the amount of energy consumed increased about 36.2~40.1%. The amount of pyrexia per hour also increased by 44.6~52.0%. During the experiment period, circulated flux was within the range of 0.48~0.49 L · s<SUP>?1</SUP> while average fluid speed was 1.53~1.56 m · s<SUP>?1</SUP>. The average temperature difference between incoming and outgoing water was 6.24~11.50℃. The amount of heating value by each set temperature within the minimum outdoor temperature range of ?14.0~?0.6℃ was 135,930~307,150 ㎉, and the range was within the 9,610~19,630 ㎉ · h<SUP>?1</SUP> per hour. This demonstrated that about 23~53% heating energy of the maximum heating load could be supplied. Total radiating value and amount of energy consumed were 2,548,306 ㎉ and 3,075.7 ㎾h, respectively. When heating takes place using oil, which is a fossil fuel, the total amount of light oil consumed was 281.6 L while the cost was 321,000 won. When the electricity cost for farms is applied, the total cost was about 110,730 won, which is about 33.5% of the cost required compared to oil consumption. The temperature at in the experiment area was about 8.3~14.6℃ higher compared to that of the control area.