Reactive Oxygen Species Generated by 17β-estradiol Play a Role in the Up-regulation of GPX4 Protein in MCF-7 Breast Cancer Cells

Sang-Han Lee,Hee Jeong Kim2,Hyo Jin Kang,이윤진,남해선,배인수 한국유방암학회 2009 Journal of breast cancer Vol.12 No.3

Purpose: Estrogen is known to act as both a growth factor and a survival factor for breast cancer. The responsible molecular mechanisms remain, however, to be fully elucidated. We hypothesize that the effect of estrogen relates to its ability to induce the cellular antioxidant defense enzymes. Methods: In the presence study, we examined the ability of 17β-estradiol (E2) to regulate the level of phospholipid hydroperoxide glutathione peroxidase (GPX4) protein, which is an anti-oxidative enzyme that can directly reduce both phospholipids and cholesterol-hydroperoxides located in the cell membranes and lipoproteins. Results: E2 elicited a dose- and time-dependent increase in the GPX4 expression in the MCF-7 breast cancer cells, and this up-regulation was blocked by the free radical scavenger N-acetylcysteine (NAC). Additionally, we confirmed that E2 triggered a rapid and transient increase in the intracellular reactive oxygen species (ROS) levels, and this E2-induced increase in the ROS levels was inhibited by pretreatment with NAC. Moreover, such ROS inducers as TGF-β, TNF-αand insulin induced an increase in the level of GPX4 protein. However, estrogen receptor (ER)αknockdown by transfection with ERα-siRNA did not significantly change the GPX4 protein level that was induced by E2. Furthermore, pre-incubation with the ER antagonist ICI 182,780 did not inhibit E2-mediated GPX4 induction. Conversely, pretreatment of cells with LY294002, a pharmacological inhibitor of phosphatidylinositol 3-kinase inhibitor, suppressed the E2-augmented GPX4 expression. Conclusion: Collectively, our data show that E2 may partly provide a survival advantage through the regulation of cellular oxidative homeostasis in MCF-7 breast cancer cells.

Korean English Teacher's Disempowerment in English-only Classes

Sung-Sam Hwang,Hyo-Sun Seo,Tae-Young Kim 한국사회언어학회 2010 사회언어학 Vol.18 No.1

Hwang, Sung-Sam, SeD, Hyo-Sun, Kim, Tae-Young. 2010. Korean English Teacher's Disempowerment in English-only Classes: A Case Study Focusing on Korea-specific Cultural Aspects. The Sociolinguistic journal of Korea 18(1). In this case study, we have focused on the disempowerment of a Korean English teacher (KET) in English-only classrooms. Six English as a foreign language (EFL) classrooms in a Korean high school were observed and analyzed by using interpretive discourse analysis. Out of the six classes, three representative classrooms were both video- and audio-taped. The results show that the English-only classes could weaken the KETs' power via three mechanisms: (a) calling the KETs' last name for choral greetings at the beginning of the class, (b) students' group solidarity and peer pressure between classmates, and (c) the students' excessively collaborative approach to peer assistance. It is argued that the exclusive English-only instruction disempowers the KETs' status and dismantles their vocational identity. In order to remedy the current situation, within a scaffolding supported by the eclectic use of first language where necessary for effective class management, KETs are empowered and overcome constraints imposed by the limited L2 oral competence of some non-native English teachers.

전래동화에 기초한 장단-말놀이 활동이 유아의 언어능력, 국악능력, 그리기표상능력에 미치는 효과

장효지(Hyo-Ji Jang),김성희(Sung-Hee Kim) 중앙대학교 한국교육문제연구소 2016 한국교육문제연구 Vol.34 No.2

본 연구의 목적은 만 5세 유아를 대상으로 전래동화에 기초한 장단-말놀이 활동을 개발하고 유아의 언어능력, 국악능력, 그리기표상능력에 미치는 효과를 분석하여 유아교육 현장 적용 및 타당성을 검증하는데 있다. 연구대상은 K시에 소재한 실험집단 C유치원 만 5세 유아 20명과 비교집단 R유치원 만 5세 유아 20명을 연구 대상으로 선정하였으며 연구대상의 평균연령은 실험집단 72.05개월, 비교집단 72.40개월로 동질 집단인 것으로 나타났다. 연구도구는 ‘구문의미 이해력 검사’(서울장애인종합복지관, 2009), 유아국악능력 검사 도구(박형신, 2006), 그리기표상능력(지성애, 2001)을 사용하였다. 연구결과는 SPSS 18.0 프로그램을 사용하여 공변량분석(ANCOVA)를 실시하였다. 본 연구 결과는 ‘전래동화에 기초한 장단-말놀이 활동’은 유아의 언어능력, 국악능력 증진에 효과적인 것으로 밝혀졌다. The purpose of this study is to analyze the effects of a Korean folktale based rhythm-word play activity on children’s language, drawing representation, and traditional Korean music ability in an early childhood education. ‘The Construction Meaning Comprehension Test’ is used to measure children’s language ability and it was developed and standardized by Seoul Community Rehabilitation Center(2009), ‘Traditional Korean Music Ability Test’ developed by Park Hyung-Shin(2006), ‘The Drawing Representation Inventory’ developed by Chi Sung-Ae(2001). In conclusion, a ‘Korean folktale based rhythm-word play activity’ has positive effects on language and traditional Korean music ability development. This study proves the validity of applying ‘ a Korean folktale based rhythm-word play activity’ in young children's education. Therefore, children’s language ability and traditional Korean music ability can be effectively increased by actively applying ‘a Korean folktale based rhythm-word play activity’ teaching-learning method in young children's education.

A Functional Polymorphism in the CHRNA3 Gene and Risk of Chronic Obstructive Pulmonary Disease in a Korean Population

유승수,( Hyo Gyoung Kang ),( Jin Eun Choi ),( Hyo Sung Jeon ),( Jae Hee Lee ),( Shin Yup Lee ),( Seung Ick Cha ),( Chang Ho Kim1 ),( Jae Yong Park ) 대한결핵 및 호흡기학회 2012 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.114 No.-

Purpose: A genome-wide association study has identified the 15q25 region as being associated with the risk of chronic obstructive pulmonary disease (COPD) in Caucasians. This study intended as a confirmatory assessment of this association in a Korean population. Methods: The rs6495309C>T polymorphism in the promoter of nicotinic acetylcholine receptor alpha subunit 3 (CHRNA3) gene was investigated in a case-control study that consisted of 406 patients with COPD and 394 healthy control subjects. Results: The rs6495309 CT or TT genotype was associated with a significantly decreased risk of COPD when compared to the rs6495309 CC genotype (adjusted odds ratio=0.69, 95% confidence interval=0.50-0.95, P=0.02). The effect of the rs6495309 C>T on the risk of COPD was more evident in moderate to very severe COPD than in mild COPD under a dominant model for the variant T allele (P-value of test for homogeneity=0.02). Conclusions: We confirmed the association between the 15q25 region and the risk of COPD in a Korean population.

A Case of Favorable Responses after Gefitinib in a Patient with EGFR Mutated Adenosquamous Lung Carcinoma

Nam Jun Cho,Ho-Sung Lee,Si-Hyong Jang,Jae Sung Choi,Ju Ock Na,Ki Hyun Seo,Yong Hoon Kim,Hyo-Chul Kang,Dae-Yeon Kim1 순천향의학연구소 2013 Journal of Soonchunhyang Medical Science Vol.19 No.2

In patients with advanced non-small cell lung cancer harboring epidermal growth factor receptor (EGFR) mutations, the epidermal growth factor receptor-tyrosine kinase inhibitors (EGFR-TKIs) are used as first treatment option. Because adenosquamous carcinoma (ASC) is a rare histologic subtype, evidences about EGFR-TKIs as first treatment option for advanced ASC are lacking. We report a case of an advanced ASC patient with the EGFR mutation, who showed good responses during 4-month treatment with gefitinib. And we will review about a necessity of EGFR mutation test and efficacy of EGFR-TKIs in ASC patients from the recent studies.

Do We Still Use The Camitta"s Criteria For Severe Aplastic Anemia?

( Hyun Hwa Yoon1 ),( Sung Hyun Kim1 ),( Ji Hyun Lee ),( Seok Jae Huh ),( Suee Lee ),( Sung Yong Oh ),( Hyuk Chan Kwon ),( Hyo Jin Kim ) 대한내과학회 2011 대한내과학회 추계학술대회 Vol.2011 No.-

장기표면의 내외봉한관과 봉한소체의 형태학적 관찰

안성훈 ( Seong Hun Ahn ),김민수 ( Min Su Kim ),이상훈 ( Sang Hun Lee ),권오상 ( O Sang Kwon ),김재효 ( Jae Hyo Kim ),소광섭 ( Kwang Sup Soh ),손인철 ( In Chul Sohn ) 대한경락경혈학회 2009 Korean Journal of Acupuncture Vol.26 No.1

Objective: In 1960`s Bonghan Kim`s team found BongHan(BH) ducts which were presumed as acupuncture meridians and BH corpuscles. They asserted Bonghan theory and SanAl theory which was involved in cell division and cell restoration. However, many other experiments which had been operated to demonstrate and find the existence of BH ducts had failed because of the secret of blue stain drugs. During the last several years, BongHan theory has been revived through experimental researches to find the anatomical structures of BH ducts and corpuscles by Soh`s Biomedical Physics Lab. Soh`s research team used the staining with Janus Green B, Alcian blue, nanoparticles and Acridine Orange. We used DAPI staining to find the existence of BH ducts and the corpuscles and to observe nuclear arrangement. Methods: We used japan white rabbits as experimental animals. BH ducts and corpuscles were stained with DAPI. The nucleus configuration in BH ducts stained with DAPI were observed with microscope. Results: In this study, we found thread like structures in silver white color distinguished from the blood vessels, nerves and lymph vessels. These thread like vessels in the linear duct shape were connected to same colored mass in the ball shape. Thread like structures we found could be separated easily from the surrounding other organ mass. The nuclei of the thread like structure in DAPI staining, are about 10~20㎛ length, in rod shape and linear arrangement. Conclusion: We concluded that the thread like structure we found was same vessel reported by Soh`s research team, BongHan ducts and corpuscle.

견관절 불안정성 환자를 대상으로 한 부하 방사선 사진의 임상적 유용성에 대한 평가

박진영(Jin-Young Park),김영복(Youngbok Kim),임화경(Hwa-Kyung Lim),문상원(Sang Won Moon),방진영(Jin-Young Bang),장효석(Hyo-Seok Jang) 대한정형외과학회 2016 대한정형외과학회지 Vol.51 No.4

목적: 견관절 불안정성 환자에서 부하 방사선 사진의 임상적 유용성을 평가하였다. 대상 및 방법: 56명 환자와 20명 정상인에서 90도 외전과 0도 외회전에서 전후방으로(AER0/PER0), 외회전만 60도로 하여 전후방으로(AER60/PER60) 15 daN의 힘을 가하여 전위를 분석하고 신체검사법과 비교하였다. 결과: 환자군의 환측에서 모든 사진이 유의한 큰 전위를 보였고(p<0.05) AER60이 유의한 최소 전위를 보였으나(p<0.05) 건측에서는 특이 전위는 없었다(p=0.27). 정상인과의 비교에서 환측 PER0, PER60만 유의한 큰 전위를 보였다(p<0.05). 신체검사법은 16명의 전방 탈구 환자에서 마취하 anterior drawer test와 AER0가 양의 상관관계를 보였고(Spearman rho=0.56, p<0.024), 환자를 3 mm 전위를 기준으로 재구분하였을 때 load and shift test의 등급이 사진상의 전방 전위와(p=0.028), Kim’s test 양성 빈도가 후방전위와 상관성을 보였다(p=0.005). 결론: Telos GA-IIE 견관절 부하 방사선 사진은 건측과 환측을 구분할 수 있고 신체 검사법과 일부 상관성을 보였다. Purpose: The purpose of this study is to examine the clinical applicability of stress radiography in patients presenting with shoulder instability. Materials and Methods: Fifty-six patients diagnosed with shoulder instability and 20 healthy volunteers participated in the study. Degree of displacement of the humeral head as measured on stress radiography using a Telos GA-IIE device was compared with the results of the physical examinations. Four types of stress radiography were captured while applying 15 daN of force anteriorly (AER0 and AER60) and posteriorly (PER0 and PER60) at two different positions: (1) 90° of abduction combined with 0° of external rotation, and (2) 90° of abduction combined with 60° of external rotation. Results: The degree of displacement of affected shoulders of 44 patients showed significantly larger displacement than normal shoulders (p<0.05), and the comparison between 56 affected shoulders of the patients and 40 normal shoulders of the volunteers showed significantly larger displacement only in PER0 and PER60 of the patients (p<0.05). Among the four radiographs of affected shoulders, AER60 showed significantly less displacement (p=0.046). The anterior drawer test under anesthesia of 16 patients who underwent surgery for anterior instability showed positive correlation with AER0 (Spearman’s rho=0.56, p<0.024). Significantly larger anterior displacement of the load and shift test was observed in the subgroup with anterior displacement more than 3 mm (p=0.028), and higher positive frequency of the Kim’s test was observed in the subgroup with posterior displacement more than 3 mm (p=0.005). Conclusion: Stress radiography using a Telos GA-IIE device could discriminate the affected shoulder. Although it could not replace individual physical examinations, the degree of displacement correlates with some physical examinations for shoulder instability.

Recent Advancements in the Pediatric Liver Transplantation : A Single-center Study of 235 Patients Over 27 Years

( Sung-woo Ahn ),( Nam-joon Yi ),( Kyung Chul Yoon ),( Suk Kyun Hong ),( Hyo-sin Kim ),( Hyeyoung Kim ),( Youngrok Choi ),( Kwang-woong Lee ),( Kyung-suk Suh ) 대한간학회 2016 춘·추계 학술대회 (KASL) Vol.2016 No.1

Purpose: Pediatric liver transplantation (PLT) has been the key therapy for end stage liver disease and the outcome has been excellent. However, still surgical complication associated with small recipient is the main cause of graft loss. In the present study, we assessed recent advances in outcome of PLTs through our experience. Methods: A total of 235 PLTs performed between Mar 1988 and July 2015 were analyzed. Three chronological periods were investigated: the initial period (1988-2007, n=117), the mid-term period in which our PLT management protocol was settled down (2008-2011, n=62), and the period in which surgical procedures were refined for small children (2012-2015, n=56). Results: The grafts’ (84% vs. 82% vs. 98%) and patients’ (84% vs. 87% vs. 98%) survival have been improved (p<0.05), whereas the number of biliary atresia (31% vs. 45% vs. 50%) deceased and the proportion of deceased donor (24% vs. 36% vs. 52%) and split PLT (7%vs. 16% vs. 46%) increased (p<0.05). The number of re-LT (3% vs. 9% vs. 2%) has been changed. ABO incompatible PLT has introduced on the last period (9%). Conclusion: The quality of the PLT has recently been standardized through a large volume of experience, and the operation has been proven to improve the survival outcome. However, a constant evaluation of our experience is critical for further progress.

2D WPS System for Measuring the Location Changes in Real Time of PAL-XFEL Devices

Hyo-Jin Choi,Sang-Bong Lee,Hong-Gi Lee,SoungYoul Back,Seung-Hwan Kim,Heung-Sik Kang 한국물리학회 2018 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.73 No.8

Several parts that comprise the large scientific equipment should be installed and operated at precise three-dimensional location coordinates X, Y , and Z through survey and alignment to ensure their optimal performance. As time goes by, however, the ground uplifts and subsides, which changes the coordinates of the installed components and leads to alignment errors X, Y , and Z. As a result, the system parameters change, and the performance of the large scientific equipment deteriorates accordingly. Measuring the change in the locations of the systems comprising the large scientific equipment in real time would make it possible to predict alignment errors, locate any region with greater changes, realign components in the fast changing region and shorten the time of survey and alignment. For this purpose, a hydrostatic leveling sensor (HLS) with 0.2 m resolution was installed and is operated in the PAL-XFEL building. In addition, a 2D wire position sensor (WPS) with a 0.1 m resolution was installed and is operated in the undulator sections where measurements of the two-dimensional changes of the systems (vertical & horizontal) are necessary. This paper is designed to introduce the operating principle of the 2D WPS, the installation and operation of the WPS system, and the way in which the WPS system is utilized in order to ensure beam stability.