Polyurethane의 혈전방지처리가 Staphylococcus epidermidis의 정착에 미치는 영향

김성민,이규백,백경란,오명돈,민병구,최강원 대한감염학회 1996 감염 Vol.28 No.1

목적 : 최근 생체재료로 만든 각종 카테타와 인공관절, 인공심장 등 인공장치의 사용이 점차 증가하고 있으며, 인공장치의 사용을 제한하는 가장 큰 합병증은 생체재료를 중심으로 일어나는 감염과 혈전증이다. 그러므로 인공장치를 장기간 사용하기 위해, 이들 합병증을 예방할 수 있는 생체재료를 개발하는 것이 중요한 과제가 되고 있다. 인공장치는 체내에 들어가서 혈액과 접촉하게 되면, 혈장단백질과 혈소판이 부착하고 활성화되어 혈소판-섬유소 혈전을 만든다. 이렇게 형성된 혈전은 세균의 부착을 촉진하고, 부착한 세균이 인체방어기전이나 항균제의 영향을 받지 않고 증식 할 수 있는 공간이 된다. 또한 정착한 세균은 혈액과 접촉하여, 혈전의 형성을 촉진한다. 즉, 혈전과 세균정착은 서로 영향을 주면서 인공자치의 오랜 사용을 제한하게 된다. 폴리우레탄에 설폰산과 알부민을 표면처리하면 혈전의 형성이 감소하는 효과가 있다는 것은 보고된 바 있으나, 혈전방지를 위해서 표면처리에 사용한 설폰산, 알부민이 세균 정착 및 감염에 미치는 영향에 대해서는 아직 잘 알려져 있지 않다. 이에 저자들은 설폰산, 알부민, 파라벤으로 처리한 폴리우레탄이 세균의 정착에 미치는 영향에 대해 알고자 본 연구를 시행하였다. 방법 : S. epidermidis RP12 부유액(?/mL)에 대조 폴리우레탄과 설폰산과 알부민, paraben으로 각각 처리한 폴리우레탄을 원반모양으로 만들어 담근 다음, 24시간 또는 48시간 동안 배양하였다. 세균 정착의 정도는 세균 집락수 및 SEM 소견으로 판정하였다. 결과 : 24시간 째 및 48시간 째의 세균집락수는 대조 폴리우레탄이 각각 8.3x10⁴/mL, 1.1×?/mL이었고, 파라벤 처리 폴리우레탄이 각각 9.3x10⁴/mL, 2.1x10⁴/mL (p<0.05), 설폰산 처리 폴리우레탄이 각각 1.2×?/mL, 8.2x10⁴/mL,(p<0.05), 알부민 처리 폴리우레탄이 각각 7.3×?/mL, 1,4×?/mL (p<0.05)이었다. 즉, 세균 집락수가 파라벤 처리 폴리우레탄에서는 유의하게 감소하였으나, 설폰산 처리 폴리우레탄에서는 증가하였다. SEM 소견에서는 설폰산 및 알부민 처리 폴리우레탄에서 세균 정착의 감소를 관찰하지는 못하였다. 결론: 본 연구는 in vitro 실험으로, 파라벤으로 처리한 폴리우레탄은 세균의 정착을 감소시키는 효과가 있었으나, 항혈전성 폴리우레탄은 세균 정착을 감소시키지 못하였다. 인체내에서는 세균과 혈소판, 혈장단백질이 복잡하게 반응을 하면서 혈전과 세균 정착이 서로 영향을 주므로, 인체내에서는 항혈전성 폴리우레탄이 혈전 형성을 감소시키고, 2차적으로 세균 정착을 감소시키고 감염을 예방하는 효과가 있을 가능성도 있으므로 이에 대한 연구가 진행되어야 하겠다. Background : Infection and thromboembolism are major complications of prolonged use of prosthetic devices, such as various catheters, prosthetic joints, and artificial hearts, frequently resulting in severe morbidity, or death. Therefore, the prevention of infection and thrombosis is an important subject in developing new biomaterials of prosthetic devices. The first event on insertion of a prosthetic device into the blood is protein adsorption and activation with platelet adhesion, activation and aggregation, leading to the formation of platelet-fibrin thrombi. The thrombi are susceptible to bacterial incorporation and protect the bacteria from the effects of circulating antibodies, phagocytic cells, and antibiotics. Furthermore, adherent bacteria may induce fibrin and platelet adhesion. Sulfonated polyurethane and albumin-immobilized polyurethane were documented to diminish the adhesion and shape-change of platelet on the polymer in comparison with plain polyurethane, in vitro. The objective of this study was to evaluate the effect of these antithrombotic biomaterials on the bacterial colonization. Methods : Plain, sulfonated, albumin-immobilized, and paraben-incorporated polyurethane(PU) films were prepared as a disk form. Staphylococcus epidermidis RP12 was suspended at a concentration of ?/mL and four types of polymeric disks were immersed in the bacterial suspension. After incubation for 24 and 48 hours, the bacterial colonization on the each disk was determined by the colony forming units(CFU) counting method. The colonized disks were examined by scanning EM. Results : The paraben decreased significantly the number of CFU on PU surface to 2.1x10⁴/mL after 48 hours, compared with 1.1×?/mL on plain polymers. The number of CFU on sulfonated or albumin-immobilized PU was 8.2x10⁴/mL, and 1.4×?/mL, respectively. SEM micrographs showed no reduction of colonization on antithrombotic PUs. Conclusion : Antithrombotic treatments of polymers did not decrease the bacterial colonization on polymers, in vitro, that was compared to the decreasing effect of paraben. However, because the thrombi and bacteria interact with each other, the overall effect of the antithrombotic polyurethanes on the bacterial colonization and infection should be studied in vivo.

대전·충남지역 고등학교 1학년생의 풍진 항체보유율과 백신접종 후의 항체형성률

오성균,김수영,여경오,김윤태,최명한,이석구,조영채,이동배,이태용 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.2

For the purpose of investigating the positive rate of rubella antibody and sero-conversion rate after rubella vaccination, 863 high school students of the two regions were studied from April 21st, 1996 to September 20th, 1996. The main results were as follows; 1. Positive rate of IgG in male was higher than in female, and the rate of IgM was also higher in male than in female. 2. Positive rate of IgG by blood type was similar, but positive rate of IgM was higher in AB type than the others. 3. Positive rate of IgG by vaccination was similar to all groups, but positive rate of IgM was higher in which was not vaccinated group. 4. Positive rate of IgG and IgM, having vaccination card, was less than which was not having vaccination card. 5. Those who were vaccinated acquired IgG(+) regardless of an existence of IgG antibody, and 28.3% of IgG(-), IgM(+) male who was not vaccinated became IgM(+), IgG(+) after 3months later. As a result positive rate of male was higher than female. The author conclude that vaccination is necessary to prevent from rubella for them.

청정지역과 도시지역의 오존농도 특성 연구

장광미,강창희,서명석,허철구,박경윤,이호근,김영준 濟州大學校 基礎科學硏究所 1996 基礎科學硏究 Vol.9 No.2

A study has been performed on the characteristics of rural and urban surface ozone concentration for the period of March 1992 to February 1993. The monitoring station of rural ozone is located at Kosan, Cheju and other urban monitoring stations are located at Seoul, Pusan and Kwangju. Rural's and urban's ozone data exhibit a distinct features in many ways. First, annual mean of rural ozone concentration is very high(42 ppbv) but urban's are very low(10~15 ppbv). Second, rural ozone data shows a seasonal variation with it's maximum in spring, and minimum in summer, but urban's show a seasonal variation with it's maximum in spring, and minimum in winter. Third, diurnal variation of rural data is very small but that of urban's are very large. Fourth, monthly mean of daily minimum of rural data is not low and shows a large seasonal variation, but those of urban's data are extremely low(<3 ppbv) and have no seasonal variations.

CDK2AP1, a Cyclin-Dependent Kinase 2-Associated Protein, Interacts with Kinesin-1 through Kinesin Superfamily Protein 5A (KIF5A)

Myoung Hun Kim(김명훈),Se Young Pyo(표세영),Young Joo Jeong(정영주),Sung Woo Park(박성우),Mi Kyoung Seo(서미경),Won Hee Lee(이원희),Sang-Hwa Urm(엄상화),Mooseong Kim(김무성),Jung Goo Lee(이정구),Dae-Hyun Seog(석대현) 한국생명과학회 2023 생명과학회지 Vol.33 No.7

세포 내 수송 및 축삭 수송은 kinesin 및 cytoplasmic dynein과 같은 미세소관 의존성 모터단백질에 의해 운반된다. Kinesin은 미세소관을 따라 미세소관의 플러스 쪽 끝으로 이동하고, dynein은 미세소관의 마이너스 쪽 끝으로 이동한다. Kinesin-1은 kinesin superfamily protein (KIF)중에서 처음으로 확인된 kinesin으로, 카복실(C)-말단 영역과 cargo간 결합을 통해 세포내 소기관, 신경전달물질 수용체 및 mRNA-단백질 복합체를 포함한 다양한 cargo의 세포내 수송 기능을 수행한다. Kinesin-1은 다양한 cargo들을 수송하지만, kinsin-1과 cargo 사이를 매개하는 어댑터/스캐폴더 단백질은 아직 완전히 확인되지 않았다. KIF5A의 C-말단 영역과 상호 작용하는 어댑터 단백질을 규명하기 위해 효모 2-하이브리드 스크리닝을 하여, cyclin-dependent kinase 2-associated protein 1 (CDK2AP1)를 확인하였다. CDK2AP1은 KIF5A의 C-말단 영역에 결합하고 KIF3A, KIF5B, KIF5C 및 kinesin light chain 1 (KLC1)과는 결합하지 않았다. CDK2AP1의 C-말단 영역은 KIF5A와의 결합에 필수적이었다. HEK-293T 세포에 CDK2AP1 및 kinesin-1은 동시 발현하여 면역침강하면 CDK2AP1 및 kinesin-1은 같이 면역침강하였다. 그리고 CDK2AP1 및 kinesin-1은 세포내에서도 같은 위치에 발현하였다. 이러한 결과들은 KIF5A-CDK2AP1결합은 kinesin-1이 cargo를 운반할 때 kinesin-1과 cargo 사이를 연결하는 어댑터 단백질 역할을 시사한다. Intracellular and axonal transport is mediated by microtubule-dependent motor proteins, such as kinesins and cytoplasmic dynein. Kinesin moves along the microtubule to the positive end of the microtubule, while dynein moves to the negative end of the microtubule. Kinesin-1 was first identified as a kinesin superfamily protein (KIF) that functions in the intracellular transport of various cargoes, including organelles, neurotransmitter receptors, and mRNA-protein complexes, through interactions between the carboxyl (C)-terminal domain and the cargo. It interacts with other cargoes, but the adapter/scaffold proteins that mediate between kinesin-1 and the cargo have yet to be fully identified. In this study, a yeast two-hybrid screen was used to identify adapter proteins that interact with the C-terminal region of KIF5A. We found an association between the C-terminal region of KIF5A and the cyclin-dependent kinase 2-associated protein 1 (CDK2AP1), originally identified in malignant hamster oral keratinocytes. CDK2AP1 bound to the C-terminal region of KIF5A and did not interact with KIF3A (the motor of kinesin-2), KIF5B, KIF5C, and kinesin light chain 1 (KLC1). The C-terminal region of CDK2AP1 is essential for its interaction with KIF5A. When co-expressed in HEK-293T cells, CDK2AP1 and kinesin-1 co-immunoprecipitated and co-localized in the cells. These results suggest that the KIF5A-CDK2AP1 interaction serves as an adapter protein connecting kinesin-1 and the cargo when kinesin-1 transports cargo in cells.

Matrix-Assisted Laser Desorption/Ionization Time-of-Flight (MALDI-TOF)-Based Cloning of Enolase, ENO1, from Cryphonectria parasitica

( Myoung Ju Kim ),( Hea Jong Chung ),( Seung Moon Park ),( Sung Goo Park ),( Dae Kyun Chung ),( Moon Sik Yang ),( Dae Hyuk Kim ) 한국미생물생명공학회 2004 Journal of microbiology and biotechnology Vol.14 No.3

First Record of the Giant Grouper Epinephelus lanceolatus (Perciformes: Serranidae: Epinephelinae) from Jeju Island, South Korea

Myoung, Jung-Goo,Kang, Chung-Bae,Yoo, Jae Myung,Lee, Eun Kyung,Kim, Sung,Jeong, Choong-Hoon,Kim, Byung-Il The Korean Society of Fisheries and Aquatic Scienc 2013 Fisheries and Aquatic Sciences Vol.16 No.1

A single specimen of Epinephelus lanceolatus, belonging to Epinephelinae of the family Serranidae, was newly collected from Jeju Island, South Korea, in December 2009. Epinephelus lanceolatus is characterized by anterior lateral-line scales with branched tubules, cycloid scales, irregular white spots, and fins with numerous small black spots. We propose a Korean name, "Dae-wang-ba-ri' for this species.

Evaluation of Anti-inflammatory Components in Glycyrrhizae Radix and Identification of the Active Ingredients

KIM Yeong Shik,SHIN Eun Myoung,GOO Lianyu,HONG Yoo Na,CHOI Yong Hwan,KIM Hyun Pyo,KIM Jeong Ah,LEE Seung Ho,KANG Sam Shik 大韓藥學會 2006 大韓藥學會 總會 및 學術大會 Vol.2006 No.1

Experimental Study on Behavior Characteristics in Crankshaft Oil Seal

Han Goo Kim(김한구),Sae-il Jeon(전세일),Myung Koo Kim(김명구),Akio Nakamura,Se Jong Yoo(유세종),Mun-Ha Lee(이문하),Myoung-Hun Oh(오명훈),Jung-Kyou Kim(김정규) 한국자동차공학회 2006 한국자동차공학회 춘 추계 학술대회 논문집 Vol.- No.-

The behavior characteristics of the gasoline engine crankshaft oil seal regarding the assembling condition are being experimentally studied. Normally, most of the crankshaft oil seals are assembled onto the housing which is created after the cylinder block and the oil pan are assembled. Since the cylinder block and the oil pan are separate parts, mismatching quantity presence cannot be avoided which has influence on the crankshaft oil seal behavior characteristics. Thus, experimental study has been taken place for the leakage characteristic and the friction torque value change according to the mismatching amount variance and the engine oil temperature with the shaft velocities. The result shows that when the rotating shafts velocity is constant, leakage was present when it was over 0.6 ㎜ and friction torque increases proportional to the mismatching amount. From the experimental study, it was relevant that the assembling condition of the cylinder block and the oil pan has effects on the behavior characteristics for the crankshaft oil seal.

적응 퍼지 논리를 이용한 Mobile Vehicle의 Lateral 제어기 설계 및 적용

김명중(Myoung-Joong Kim),임형순(Hyung-soon Lim),이창구(Chang-Goo Lee),김성중(Sung-Joong Kim) 대한전기학회 1999 대한전기학회 학술대회 논문집 Vol.1999 No.7

흡음체를 이용한 ISO type 차음시험실의 음장조정에 관한 연구

김명수(Myoung Soo Kim),김형진(Hyung Jin Kim),구희모(Hee Mo Goo),김의간(Ue Kan Kim) 한국항해항만학회 2008 한국항해항만학회 학술대회논문집 Vol.2008 No.공동학술