Brachybacterium kimchii sp. nov. and Brachybacterium halotolerans subsp. kimchii subsp. nov., isolated from the Korean fermented vegetables, kimchi, and description of Brachybacterium halotolerans subsp. halotolerans subsp. nov.

Yujin Kim,Yeon Bee Kim,Juseok Kim,Joon Yong Kim,Tae Woong Whon,Won-Hyong Chung,Eun-Ji Song,Young-Do Nam,Se Hee Lee,Seong Woon Roh 한국미생물학회 2022 The journal of microbiology Vol.60 No.7

Two Gram-stain-positive, oxidase-negative, catalase-positive, and coccus-shaped bacterial strains, designated CBA3104T and CBA3105T, were isolated from kimchi. Strain CBA3104T and CBA3105T grew at 10–35°C (optimum, 25°C and 30°C, respectively), at pH 6.0–8.5 (optimum, pH 6.5), and in the presence of 0–15% (w/v) NaCl (optimum, 5%). A phylogenetic analysis based on 16S rRNA gene sequences revealed that strain CBA3104T formed a distinct phylogenetic lineage within the genus Brachybacterium whereas strain CBA3105T was closely positioned with Brachybacterium halotolerans MASK1Z-5T. The 16S rRNA gene sequence similarity between strains CBA3104T and CBA3105T was 99.9%, but ANI and dDDH values between strains CBA3104T and CBA3105T were 93.61% and 51.5%, respectively. Strain CBA3104T showed lower ANI and dDDH values than species delineation against three closely related strains and type species of the genus Brachybacterium, however, strain CBA3105T showed 96.63% ANI value and 69.6% dDDH value with Brachybacterium halotolerans MASK1Z-5T. Among biochemical analysis results, strain CBA3104T could uniquely utilize bromo-succinic acid whereas only strain CBA3105T was positive for alkaline phosphatase and α-fucosidase among two novel strains, closely related strains, and type species of the genus Brachybacterium. Compared with strain CBA3105T and Brachybacterium halotolerans JCM 34339T, strain CBA3105T was differentially positive for acid production of D-arabinose, D-adonitol, and potassium 5-ketogluconate and enzyme activity of β-glucuronidase. Both strains contained menaquinone-7 as the dominant quinone. The cell-wall peptidoglycan of two novel strains contained meso-diaminopimelic acid. The major fatty acids of strains CBA3104T and CBA3105T were anteiso-C15:0, anteiso-C17:0, and iso-C16:0. The major polar lipids of both strains were phosphatidylglycerol and diphosphatidylglycerol. Strain CBA3104T possessed a uniquely higher abundance of tRNA (97 tRNAs) than four Brachybacterium strains used for comparative taxonomic analysis (54–62 tRNAs). Both the CBA3104T and CBA3105T strain harbored various oxidoreductase, transferase, hydrolase, and lyase as strain-specific functional genes compared to closely related strains and Brachybacterium type species. The results of biochemical/physiological, chemotaxonomic, and genomic analyses demonstrated that strains CBA3104T and CBA3105T represent a novel species of the genus Brachybacterium and a novel subspecies of B. halotolerans, respectively, for which the names Brachybacterium kimchii sp. nov. and B. halotolerans subsp. kimchii subsp. nov. are proposed. The type strains of the novel species and the novel subspecies are CBA3104T (= KCCM 43417T = JCM 34759T) and CBA3105T (= KCCM 43418T = JCM 34760T), respectively.

[PA-0021] Identification of noble candidate gene associated with sensitivity to phytotoxicity of etofenprox in soybean

Ji-Min Kim(Ji-Min Kim),Jungmin Ha(Jungmin Ha),Ilseob Shin(Ilseob Shin),Juseok Lee(Juseok Lee),Jung-Ho Park(Jung-Ho Park),Jeong-Dong Lee(Jeong-Dong Lee),Sungteag Kang(Sungteag Kang) 한국육종학회 2022 한국육종학회 공동학술발표집 Vol.2022 No.-

Solvent-responsive polymer nanocapsules with controlled permeability: encapsulation and release of a fluorescent dye by swelling and deswelling

Kim, Eunju,Lee, Jiyeong,Kim, Dongwoo,Lee, Kyung Eun,Han, Sung Sik,Lim, Namseok,Kang, Juseok,Park, Chan Gyung,Kim, Kimoon Royal Society of Chemistry 2009 Chemical communications Vol.2009 No.12

<P>A solvent-responsive polymer nanocapsule swells and deswells in response to a change in solvent composition, and the permeability of the shell of the nanocapsule can be controlled simply by swelling and deswelling; encapsulation and release of a fluorescent dye is achieved by a swelling–deswelling–swelling cycle.</P> <P>Graphic Abstract</P><P>A solvent-responsive polymer nanocapsule swells and deswells in response to a change in solvent composition, and the permeability of the shell of the nanocapsule can be controlled simply by swelling and deswelling. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=b823110a'> </P>

Paracoccus jeotgali sp. nov., isolated from Korean salted and fermented shrimp

Juseok Kim,Joon Yong Kim,Hye Seon Song,In-Tae Cha,Seong Woon Roh,Se Hee Lee 한국미생물학회 2019 The journal of microbiology Vol.57 No.6

A Gram-stain-negative and facultatively aerobic bacterium, designated as strain CBA4604T, was isolated from a traditional Korean salted and fermented shrimp food (saeu-jeot). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain CBA4604T formed a clearly distinct phyletic lineage from closely related species within the genus Paracoccus. Strain CBA4604T was the most closely related to P. koreensis Ch05T (97.5% 16S rRNA gene sequence similarity) and other type strains (≤ 97.0%). The genome comprised a chromosome and two plasmids of 3,299,166 bp with 66.5% G+C content. The DNA-DNA relatedness values between strain CBA4604T and P. koreensis Ch05T, P. alcaliphilus DSM 8512T, and P. stylophorae KTW-16T were 30.5%, 22.9%, and 16.7%, respectively. Cells of the strain were short rod-shaped and oxidase- and catalase-positive. The growth of strain CBA- 4604T was observed at 10–40°C (optimum, 37°C), pH 6.0–10.0 (optimum, pH 7.0), and in the presence of 0–8.0% (w/v) NaCl (optimum, 0–2.0%). Strain CBA4604T contained ubiquinone 10 as the sole isoprenoid quinone and summed feature 8 (C18:1 ω7c/C18:1 ω6c) and C18:0 as the major cellular fatty acids. The polar lipids consisted of phosphatidylcholine, phosphatidylglycerol, diphosphatidylglycerol, phospholipid, an unidentified aminolipid, an unidentified glycolipid, and three unidentified lipids. Based on its phylogenetic, genomic, phenotypic, and chemotaxonomic features, we concluded that strain CBA- 4604T represents a novel species in the genus Paracoccus and we propose the name Paracoccus jeotgali sp. nov. The type strain is CBA4604T (= KACC 19579T = JCM 32510T).

Cosmetic outcomes of cesarean section scar; subcuticular suture versus intradermal buried suture

( Juseok Yang ),( Ki Hyung Kim ),( Yong Jung Song ),( Seung-Chul Kim ),( Nayoung Sung ),( Haneul Kim ),( Dong Hyung Lee ) 대한산부인과학회 2018 Obstetrics & Gynecology Science Vol.61 No.1

Objective The objective of the study was to compare cosmetic outcomes and overall satisfaction rate of cesarean section scar between conventional subcuticular suture and intradermal buried vertical mattress Methods Patients were enrolled to the study by chart review. A scar assessment was obtained retrospectively through a telephone survey. The patient component of the patient and observer scar assessment scale (POSAS) was utilized along with the overall satisfaction of the patient regarding their cesarean section scar and their willingness to choose the same skin closure technique when anticipating their next cesarean section. Results A total of 303 cases of cesarean section was recruited, 102 finished telephone surveys were calculated for the analyses. Subcuticular suture was regarded as control group (n=52) and intradermal buried suture as test group (n=50). The PSAS score of the test group (mean, 21.8) was lower than that of the control group (mean, 28), with a statistical significance (P=0.02). Overall satisfaction rate did not differ between the two groups. Two parameters of the PSAS score and the level of overall satisfaction showed significant correlation (Pearson’s r, -0.63; P<0.01). Conclusion We suggested the use of intradermal buried vertical mattress as a cosmetically superior skin closure method for application in cesarean sections over subcuticular stitch.

Effect of Electrolyte Composition on The Formation Behavior of Plasma Electrolytic Oxidation Films on Al1050 Alloy

Juseok Kim,Sungmo Moon,Myunghoon Oh 한국표면공학회 2017 한국표면공학회 학술발표회 초록집 Vol.2017 No.11

알루미늄 2024 합금의 플라즈마 전해산화 피막의 형성 거동 및 피막 물성에 미치는 인산나트륨 농도의 영향

김주석(Juseok Kim),신헌철(Heon-cheol Shin),문성모(Sungmo Moon) 한국표면공학회 2020 한국표면공학회지 Vol.53 No.6

Formation behavior and properties of PEO (Plasma Electrolytic Oxidation) film on AA2024 were investigated under application of pulsed current as a function of Na₃PO₄ concentration in 0.05 M Na₂SiO₃ solution by analyzing voltage–time behavior, in-situ observation of arc generation, observation of surface morphology and measurements of thickness and surface roughness. Arc generation voltage decreased with increasing Na₃PO₄ concentration. Color difference of PEO films between edge and inner part disappeared by addition of Na₃PO₄. It was also observed that size of nodules on PEO film decreased with increasing Na₃PO₄ concentration. Thickness of PEO films formed on AA2024 increased with increasing Na₃PO₄ concentration. Whereas, surface roughness of PEO films decreased with increasing Na₃PO₄ concentration up to 0.05 M of Na₃PO₄ which is attributed to the deceased size of nodules on the PEO films. However, the surface roughness increased with increasing Na₃PO₄ concentration more than 0.07 M of Na₃PO₄ which seems to be due to the formation of non-uniform PEO films with smooth surface and large size pores formed by orange-colored big arcs. The experimental results suggest that added sodium phosphate less than 0.2 M in an alkaline silicate solution can contribute to the formation of relatively thick and uniform thickness of PEO films under arc generation voltage lower than 300 V.

한국어 Hedge 문장 인식을 위한 태깅 말뭉치 및 단서어구 패턴 구축

정주석(Juseok Jeong),김준혁(Junhyeouk Kim),김해일(Haeil Kim),오성호(Sungho Oh),강신재(Sinjae Kang) 한국지능시스템학회 2011 한국지능시스템학회 학술발표 논문집 Vol.21 No.2

보험계약 체결 전 정보제공의무와 우리의 보험영업실무

김주석 ( Juseok Kim ) 고려대학교 법학연구원 2016 고려법학 Vol.0 No.81

보험상품은 무형의 추상적 서비스를 그 내용을 하고 있어 내부적 정보의 비대칭성에 따른 불완전판매의 가능성이 높은 것이 현실이다. 이러한 현실을 반영하여 최근 보험소비자 보호 문제가 세계적인 큰 조류가 되고 있다. 그 중에서도 특히 정보의 불균형을 해소하기 위해 보험자의 정보제공의무에 관한 입법화가 주목된다. 이와 관련하여 세계 주요선진국의 입법례를 살펴보고, 우리의 현행 법령내용 검토 및 정보제공의무의 입법화 필요성에 대해 보험실무를 중심으로 고찰해 보았다. 유럽 주요국가들의 경우, 유럽보험계약법준칙상의 정보제공의무 관련규정에 근거하여 각국의 사정을 고려하여 국내 입법화 또는 지침화를 통해 시행하고 있다. 영국은 오래 전부터 판례상 최대선의의 원칙에 근거하여 중요한 사항에 대한 보험자의 고지의무를 긍정해오다, 유럽지침을 반영한 ICOBS에 의해 보험자의 정보제공의무를 인정하고 있다. 그러나, 보험종류 또는 판매방식에 따라 정보제공의 내용이 상이하며, 서면 제공 시 설명의무까지는 부과되어 있지 않다. 독일은 보험계약법의 개정으로 정보제공의무와 조언의무를 인정하고 있으나, 설명의무는 부과되어 있지 않다. 정보제공의무 위반에 따른 효과에 대해서도 명확한 규정이 없다. 프랑스의 경우도 1980년대부터 생명보험과 손해보험 분야에서 필요에 따라 점진적으로 정보제공의무를 추가하여 왔으나, 위반 시 효과에 대한 규정은 존재하지 않는다. 미국은 각 주마다의 법규가 다르기 때문에 이를 일률적으로 평가할 수 없으나, 뉴욕주의 경우에 있어서는 생명보험에 대해 일정한 사항을 제공하도록 보험법에 규정을 두고 있다. 일본은 보험법상에 정보제공의무 또는 설명의무 관련 명문규정을 두고 있지는 않고, 다만, 소비자계약법이나 보험업법 등에 정보제공 또는 설명을 해줄 의무를 인정하고 있다. 이와 같이 각국은 그 법적, 경제적 환경에 따라 보험소비자 보호를 위한 보험정보제공을 법령화하고 있다고 할 수 있다. 우리나라는 상법상 약관 중요사항 설명의무를 규정하고, 보험업법 및 감독규정 등을 통해 설명의무 조항을 신설하여 부과하고 있는 상황이다. 설명의무의 이행은 필연적으로 그 설명 대상이 되는 정보의 제공을 동반할 수밖에 없다. 그러한 관점에서 우리나라는 정보제공의무를 명문화하고 있지 않지만, 상법 및 약관규제법상 약관 설명의무와 보험업법상 설명의무를 부과함으로써 다른 외국들에 비해 오히려 더 보험소비자 지향적이다. 왜냐하면 정보제공의무 사항과 설명의무 사항은 거의 유사하지만 우리나라의 경우 설명까지 행해야 하기 때문이다. 우리의 보험계약 체결 실무를 보면 수 차례의 보험설계와 상담 등을 통해 보험계약자에 대해 중요사항의 정보제공과 설명 또는 상담이 이루어지고 있는데, 세계 각국의 실무내용과 비교해볼 때 대동소이하다. 결국, 정보제공의무의 명문 규정 유무가 중요한 것이 아니라, 현행 법령내용이 영업실무에서 제대로 실천될 수 있도록 해야 한다. 다만, 상법상 약관 설명의무와 보험업법상 설명의무의 중복적 규정으로 인해 개념 및 상호관계에 관한 혼란이 존재할 수 있어 이에 관한 개선이 필요하다. 또한 실무상 보험계약자에게 제공되는 보험안내자료나 상품설명서의 내용에 대한 체계적인 정비, 설명의무의 이행과 이에 관한 입증근거 보완도 이루어져야 한다. 최근 IT산업과 핀테크산업 발전에 따라 이를 활용한 다양한 형태의 정보제공 및 설명이행의 방법도 법제화할 필요가 있다. 보험계약자로서도 본인이 원하는 보험상품에 관심을 갖고 보험모집인으로부터 보험안내자료나 가입설계서 및 상품설명서를 꼼꼼히 확인하고 계약체결을 하는 자기책임의식을 가져야 한다. 보험회사나 금융감독원 등의 공시자료 및 상담창구를 통해 본인이 필요로 하는 보험상품의 정보를 사전에 획득하고 파악할 수 있다. 이러한 상황에서 정보제공의무의 법제화만이 능사는 아니며, 오히려 실무상의 과도한 불편과 비용부담, 비효율성이 있을 수 있다. 따라서, 현행 법령 하에서 보험회사의 소비자를 위한 적절한 자율적 정보제공과 법령상 설명의무 이행, 보험소비자의 건전한 책임의식 사이의 적절한 긴장과 균형적 관계를 형성시키고, 우리의 보험 운영실태에 입각하여 책임의식 있는 보험소비자의 보호를 위한 다각적이고 실질적인 개선이 이루어져야 할 것이다. From years ago in our country there have been discussed a lot concerning with ``the disclosure duty of information by the insurer in the midst of the legislative trends for insurance consumer protection, and many insurance professors insist it is required to legislate the duty into the insurance contract law. So in this article I reviewed the obligation of providing insurance information in the relevant regulations of another countries, and then compared with our insurance law as well as insurance sales practice of insurance companies. The information duty of insurer is very important for the insurance consumer to select properly suitable insurance products for their own needs. In Europe, many countries including U.K., France, Germany, regulate information duty provisions required by the European Union`s insurance regulation, and they are applying the information duty rule to protect the insurance consumer through minimizing the information asymmetry. On the business practice, explanation about the insurance product is also provided to the customers by the insurer or intermediaries in those countries. On the other hand, in the U.S.A, it could not be told in uniform because the insurance regulations of each states are different. For instance, the New-York state has information duty provisions of an insurance law by which the insurer were obligated to provide information about insurance product especially on the life insurance part, on the contrary there is no special regulations for indemnity insurance. In Japan, the insurance contract law has no provision of the information duty except the duty of providing the policy, but the Insurance Business Act and Consumer Contract Act recognize a duty to give an explanation and information. In Korea, although the present insurance contract law(§383-3) and Insurance Business Act(§95-2) have no provisions of the information duty, but there are provisions of explanation duty about the important facts of insurance clauses and contract in those laws. Furthermore in the Insurance Business Act(§95) there is a regulation that the buyer`s guide which is provided to the prospective customer or contractor to know about the insurance product. The guide should contain specific information necessarily like as the name of insurance company or insurance intermediaries, matters connected with the rights and obligations of the insurance contract, protection written by the insurance clauses, limited payment(exceptions or exclusions), cash value on termination, and so on. I think the explanation duty in our country is very similar to the information duty of other countries theoretically and functionally, and generally the important information is provided on the process of insurance consulting in business practice. So it`s not necessary to add the information duty on the present regulations, but to improve the explanation contents regulated in Insurance Business Act and ordinance more clearly and systemically. Furthermore it is also necessary to add regulations which make insurance solicitor write the confirmation of intention and fulfill the explanation duty more actually in that act and ordinance.

이정보 시조와 여항시정문화권

김주석 ( Juseok Kim ) 성균관대학교 대동문화연구원 2015 大東文化硏究 Vol.91 No.-

이정보는 벼슬에서 물러난 이후 음악을 즐기며 閭巷市井文化圈의 가악그룹을 후원한다. 이 과정에서 그의 시조들은 이전의 사대부들이 지향하였던 시조 관습을 유지해 가는 한편, 여항시정문화권의 享有·生成 관습을 따르게 된다. ‘閣氏’는 이 시기 독특한 소재 유형으로 그 산출 기반은 여항시정문화권이다. ‘각시’는 주로 ‘妓女’를 가리키는데, ‘각시’를 통하여 風流房 現場의 향유 양상으로서의 ‘각시에게 말걸기’가 확인된다. 이정보의 시조는 ‘소재 선택’ 면에서는 ‘興味性’을, ‘소재 가공’ 면에서는 ‘戱作性’을 보인다. ‘흥미성’과 ‘희작성’은 여항시정문화권의 향유·생성 관습과 관련이있다. 이정보 역시 이러한 향유·생성 관습을 따랐다. 이때 발견되는 이정보만의 창작 전략은 ‘풍속의 연출’이다. 이정보는 연출자의 입장에서 閭巷市井의 흥미로운 인물들을 캐스팅하고 또 이들이 시조라는 구도 안에서 생동하게 함으로써 극적인 장면들을 다양하게 연출해낸다. Since retired from government service Lee Jeong-Bo(李鼎輔) enjoys music. And sponsored music group of ``YeohangSijeong cultural area``. He maintained Sijo-style of the Sadaebu(士大夫) of earlier times. Meanwhile, he accepts text customs in urban entertainment-atmosphere. For example, It can be found in ``Gaksi(閣氏)``. ``Gaksi`` is ``Ginyeo(妓女)``. ``Gaksi`` shows urban entertainment-atmosphere of ``YeohangSijeong(閭巷市井) cultural area``. The aspect of enjoyment is ``chat-up Gaksi``. Sijo of Lee Jeong-Bo shows ``choosing an interesting target`` and ``to process an interesting situation``. This is text customs of ``YeohangSijeong cultural area``. In particular, his own sijo writing strategy is ``to act out folkways``.