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金三純,朴性五,李址烈 서울여자대학교 1972 서울여자대학논문집 Vol.- No.2
A qualitative survey was carried out on the soil microfungi especially in Phycomycets and Genus Aspergillus from the bamboo forest soil in Naju-kun, Damyang-kun Junnam province and Jaeju Province. 16speceis of fungi were isolated by soil plate method then identified. They were Absidia glauca, Cunninghamella elegans, Gongromella sp, Mortierella elongata, Mortierella isabellina, Mortierella ramanniana var. angulispora, Mucor circinelloides, Mucor fragilis, Mucor hiemalis, Mucor racemosus,Rhizopus nigricans, Zygrohynchus heterogamus, Zygorhynchus moelleri, Aspergillus giganteus, Aspergillus niger and Aspergillus oryzae. According to the results of the Saccharogenic amylase activity,Aspegillus oryzae showed the highest value (0.728) of the absorbance and A. giganteus and Cunninghamella elegans showed the lowest value of it. On the other hand Gongronella sp, Rhizopus nigricans, Mortierella spp, Muxor spp, Zygorhynchus spp, Absidia glauca showed the values of absorbance in the range of 0.445 and 0.176 as shown in Table 5.
Kim, Mal Geum,Kang, Tae Woong,Park, Joon Yeong,Park, Seung Hun,Ji, Yun Bae,Ju, Hyeon Jin,Kwon, Doo Yeon,Kim, Young Sik,Kim, Sung Won,Lee, Bong,Choi, Hak Soo,Lee, Hai Bang,Kim, Jae Ho,Lee, Bun Yeoul,Mi Elsevier 2019 Materials science & engineering. C, Materials for Vol.103 No.-
<P><B>Abstract</B></P> <P>We have designed and characterized an injectable, electrostatically bonded, <I>in situ</I>–forming hydrogel system consisting of a cationic polyelectrolyte [(methoxy)polyethylene glycol-<I>b</I>-(poly(ε-caprolactone)-<I>ran</I>-poly(L-lactic acid)] (MP) copolymer derivatized with an amine group (MP-NH<SUB>2</SUB>) and anionic BMP2. To the best of our knowledge, there have been hardly any studies that have investigated electrostatically bonded, <I>in situ</I>–forming hydrogel systems consisting of MP-NH<SUB>2</SUB> and BMP2, with respect to how they promote <I>in vivo</I> osteogenic differentiation of human turbinate mesenchymal stem cells (hTMSCs). Injectable formulations almost immediately formed an electrostatically loaded hydrogel depot containing BMP2, upon injection into mice. The hydrogel features and stability of BMP2 inside the hydrogel were significantly affected by the electrostatic attraction between BMP2 and MP-NH<SUB>2</SUB>. Additionally, the time BMP2 spent inside the hydrogel depot was prolonged <I>in vivo</I>, as evidenced by <I>in vivo</I> near-infrared fluorescence imaging. Biocompatibility was demonstrated by the fact that hTMSCs survived <I>in vivo</I>, even after 8 weeks and even though relatively few macrophages were in the hydrogel depot. The osteogenic capacity of the electrostatically loaded hydrogel implants containing BMP2 was higher than that of a hydrogel that was simply loaded with BMP2, as evidenced by Alizarin Red S, von Kossa, and hematoxylin and eosin staining as well as osteonectin, osteopontin, osteocalcin, and type 1α collagen mRNA expression. The results confirmed that our injectable, <I>in situ</I>–forming hydrogel system, electrostatically loaded with BMP2, can enhance <I>in vivo</I> osteogenic differentiation of hTMSCs.</P> <P><B>Highlights</B></P> <P> <UL> <LI> An injectable, electrostatically bonded, <I>in situ</I>–forming hydrogel system consisting of a cationic polyelectrolyte copolymer derivatized with an amine group and anionic BMP2 was prepared. </LI> <LI> The hydrogel features and stability of BMP2 inside the hydrogel were significantly affected by the electrostatic attraction between BMP2 and cationic polyelectrolyte copolymer. </LI> <LI> The electrostatically loaded hydrogel enhanced osteogenic differentiation of human turbinate mesenchymal stem cells better than one with simple loading of BMP2. </LI> </UL> </P>
Ji Eun Sung,Jun Young Choi,Ji Eun Kim,Hyun Ah Lee,Woo Bin Yun,Jin Ju Park,Hye Ryeong Kim,Bo Ram Song,Dong Seob Kim,Chung Yeoul Lee,Hee Seob Lee,Yong Lim,Dae Youn Hwang 한국실험동물학회 2017 Laboratory Animal Research Vol.33 No.2
The inhibitory effects of Asparagus cochinchinensis against inflammatory response induced by lipopolysaccharide (LPS), substance P and phthalic anhydride (PA) treatment were recently reported for some cell lines and animal models. To evaluate the hepatotoxicity and nephrotoxicity of A. cochinchinensis toward the livers and kidneys of ICR mice, alterations in related markers including body weight, organ weight, urine composition, liver pathology and kidney pathology were analyzed in male and female ICR mice after oral administration of 150, 300 and 600 mg/kg body weight/day saponin-enriched extract of A. cochinchinensis (SEAC) for 14 days. The saponin, total flavonoid and total phenol levels were found to be 57.2, 88.5 and 102.1 mg/g in SEAC, respectively, and the scavenging activity of SEAC gradually increased in a dose-dependent manner. Moreover, body and organ weight, clinical phenotypes, urine parameters and mice mortality did not differ between the vehicle and SEAC treated group. Furthermore, no significant alterations were measured in alkaline phosphatase (ALP), alanine aminotransferase (ALT), aspartate aminotransferase (AST), lactate dehydrogenase (LDH), blood urea nitrogen (BUN) and the serum creatinine (Cr) in the SEAC treated group relative to the vehicle treated group. Moreover, the specific pathological features induced by most toxic compounds were not observed upon liver and kidney histological analysis. Overall, the results of the present study suggest that SEAC does not induce any specific toxicity in the livers and kidneys of male and female ICR mice at doses of 600 mg/kg body weight/day.
Seon, Ji-Yun,Yoon, Young Joon,Choi, Jaekyoung,Kim, Hyo Tae,Kim, Chang-Yeoul,Kim, Jong-Hee,Baik, Hong Koo American Scientific Publishers 2013 Journal of Nanoscience and Nanotechnology Vol.13 No.11
<P>A dielectophoretic (DEP) device fabricated by a conventional low temperature co-fired ceramic (LTCC) process, for manipulating micro and nanostructure materials, such as spherical polystyrene microspheres, titanium dioxide (TiO2) nanotubes, and silver (Ag) nanowires, is described. To generate a non-uniform electric field, a castellated electrode configuration was applied to the LTCC-based DEP device using a screen printing method. The actual motions of the micro and nanostructure materials under both a positive and a negative DEP force were observed in detail and the findings compared with numerical simulation data for the electric field distribution. The performance of the LTCC-based DEP device for separating and trapping was evaluated and potential applications are discussed.</P>