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Song, Kilyoung,Hyun, Sang-Hwan,Shin, Taeyoung,Lee, Eunsong Wiley Subscription Services, Inc., A Wiley Company 2009 Molecular reproduction and development Vol.76 No.7
<P>The objective of this study was to examine the effect of cytochalasin B (CB) and/or demecolcine (Dc) on the remodeling of donor nuclei, nuclear ploidy, and development of somatic cell nuclear transfer (SCNT) and parthenogenetic (PA) pig embryos. SCNT and PA oocytes were either untreated (control), or treated with CB, Dc, or both CB and Dc after electric activation, and then cultured or transferred to surrogates. In SCNT, blastocyst formation was higher after treatment with CB and/or Dc (26–28%) than in the controls (16%). The number of oocytes that formed a single pronucleus (PN) was higher after treatment with Dc (86%) and CB + Dc (86%) than under control conditions (44%) or after treatment with CB (63%). In PA, blastocyst formation was higher after CB treatment (47%) than under control conditions (28%), while the formation of a single PN was higher after treatment with Dc (88%) and CB + Dc (84%) compared to controls (34%). The rate of formation of diploid embryos was higher after treatment with Dc and CB + Dc than under control conditions. Dc treatment resulted in a farrowing rate of 50% with 1.1% production efficiency, while controls showed a farrowing rate of 37.5% and a production efficiency of 0.7%. The results of our study demonstrate that post-activation treatment with Dc improves preimplantation development and supports normal in vivo development of SCNT pig embryos, probably because Dc induces formation of a single PN and this leads to normal nuclear ploidy. Mol. Reprod. Dev. 76: 611–619, 2009. © 2008 Wiley-Liss, Inc.</P>
Eunsong Lee,Kilyoung Song 대한수의학회 2007 Journal of Veterinary Science Vol.8 No.4
The objective of the present study was to examine the feasibility of the production of autologous porcine somatic cell nuclear transfer (SCNT) blastocysts using oocytes and donor cells from slaughtered ovaries. Therefore, we attempted to optimize autologous SCNT by examining the effects of electrical fusion conditions and donor cell type on cell fusion and the development of SCNT embryos. Four types of donor cells were used: 1) denuded cumulus cells (DCCs) collected from in vitro-matured (IVM) oocytes; 2) cumulus cells collected from oocytes after 22 h of IVM and cultured for 18 h (CCCs); 3) follicular cells obtained from follicular contents and cultured for 40 h (CFCs); and 4) adult skin fibroblasts. The DCCs showed a significantly (p < 0.01) lower rate of fusion than the CCCs when two pulses of 170 V/mm DC were applied for 50 μsec (19 ± 2% vs. 77 ± 3%). The rate of DCC fusion with oocytes was increased by the application of two DC pulses of 190 V/mm for 30 μsec, although this was still lower than the rate of fusion in the CCCs (33 ± 1% vs. 80 ± 2%). The rates of cleavage (57 ± 5%) and blastocyst formation (1 ± 1%) in the DCC-derived embryos did not differ from those (55 ± 6% and 3 ± 1%, respectively) in the CCC-derived SCNT embryos. Autologous SCNT embryos derived from CFCs (5 ± 2%) showed higher levels of blastocyst formation (p < 0.01) than CCC-derived autologous SCNT embryos (1 ± 0%). In conclusion, the results of the present study show that culturing cumulus and follicular cells before SCNT enhances cell fusion with oocytes and that CFCs are superior to CCCs in the production of higher numbers of autologous SCNT blastocysts.
You, Jinyoung,Song, Kilyoung,Lee, Eunsong CSIRO Publishing 2010 Reproduction, fertility, and development Vol.22 No.6
<P> The aim of the present study was to examine the effect of various intervals between electrofusion and activation (FA interval) on the nuclear remodelling and development of somatic cell nuclear transfer (SCNT) embryos in pigs. Reconstructed oocytes were activated at 0 (simultaneous fusion and activation; SFA), 1, 2 and 3 h (delayed activation) after electrofusion; these groups were designated as DA1, DA2 and DA3, respectively. When oocyte nuclear status was examined at 0.5, 1, 2 and 3 h after electrofusion, the incidence of chromosome scattering was increased (P < 0.01) as the FA interval was extended (0.0%, 12.0%, 77.3% and 78.0%, respectively). Extending the FA interval led to an increase (P < 0.01) in the percentage of oocytes containing multiple (≥3) pseudopronuclei (PPN) (0.0% of SFA; 5.3% of DA1; 21.7% of DA2; and 33.5% of DA3). The development of SCNT embryos to the blastocyst stage was decreased (P < 0.05) in DA2 (5.7%) and DA3 (5.0%) compared with SFA (18.1%) and DA1 (19.5%). Our results demonstrate that extending the FA interval impairs the development of SCNT pig embryos by inducing chromosome scattering and the formation of multiple PPN, which may result in increased nuclear aneuploidy. </P>
Influence of Factors during Superovulation on Embryo Production in Korean Holstein Cattle
LEE, WonYou,SONG, KilYoung,LIM, KwangTaek,LEE, SongJeon,LEE, ByeongChun,JANG, Goo Japanese Society of Veterinary Science 2012 The Journal of veterinary medical science Vol.74 No.2
<P>The aim of this study was to investigate the influence of key parameters (donor parity, milk production, post-parturient day, season and milk recording data) associated with efficiency of embryo recovery (ER) in Holstein cattle. Elite Holstein cows and heifers were selected for ER, while Holstein heifers were used as recipients. The numbers of transferable embryos (TEs) produced were not significantly different when analyzed in terms of donor parity, milk production, postparturient day and season. However, the numbers of TEs were significantly increased when the milk protein (%; P)/fat (%; F) ratio was over 0.95 and/or the milk urea nitrogen (MUN) was between 12 and 18 d<I>l</I>/m<I>l</I>. The results from ET showed no differences in pregnancy rates among Holstein heifers receiving other types, developmental stage codes and quality grades of embryos. The mean interval from ER to artificial insemination was 60.6 days. Moreover, 19 offspring that had milk recording data showed a similar milk yield performance to that of the donor cows. In conclusion, this study showed that in Holstein cows, embryos were recovered and transferred and resulted in production of viable calves. Furthermore, P/F ratio and MUN could be candidate indicators for selection of high-efficiency donor cows.</P>
Eunsong Lee,Kilyoung Song 대한수의학회 2007 Journal of Veterinary Science Vol.8 No.1
This study examined effects on the developmental competence of pig oocytes after somatic cell nuclear transfer (SCNT) or parthenogenetic activation (PA) of : 1) co-culturing of oocytes with follicular shell pieces (FSP) during in vitro maturation (IVM); 2) different durations of maturation; and 3) defined maturation medium supplemented with polyvinyl alcohol (PVA; control), pig follicular fluid (pFF), cysteamine (CYS), or β-mercaptoethanol (β-ME). The proportion of metaphase II oocytes was increased (p < 0.05) by co-culturing with FSP compared to control oocytes (98% vs. 94%). However, blastocyst formation after SCNT was not improved by FSP coculture (9% vs. 12%). Nuclear maturation of oocytes matured for 39 or 42 h was higher (p < 0.05) than that of oocytes matured for 36 h (95-96% vs. 79%). Cleavage (83%) and blastocyst formation (26%) were significantly higher (p < 0.05) in oocytes matured for 42 h than in other groups. Supplementation of a defined maturation medium with 100 μM CYS or 100 μM β-ME showed no stimulatory effect on oocyte maturation, embryo cleavage, or blastocyst formation after PA. β-ME treatment during IVM decreased embryo cleavage after SCNT compared to pFF or PVA treatments, but no significant difference was found in blastocyst formation (7-16%) among the four treatment groups. The results indicated that maturation of oocytes for 42 h was beneficial for the development of SCNT embryos. Furthermore, the defined maturation system used in this study could support in vitro development of PA or SCNT embryos.
Spectroscopy-H*(10) Rate Conversion Function G(E) for a Plastic Scintillation Detector
Jisung Hwang,Junhyeok Kim,Kilyoung Ko,Wonku Kim,Jaehyun Park,Gyohyeok Song,Sangho Lee,Gyuseong Cho 한국방사성폐기물학회 2022 한국방사성폐기물학회 학술논문요약집 Vol.20 No.2
Dose-rate monitoring instruments are indispensable to protect workers from the potential risk of radiation exposure, and are commonly calibrated in terms of the ambient dose equivalent (H*(10)), an operational quantity that is widely used for area monitoring. Plastic scintillation detectors are ideal equipment for dosimetry because of their advantages of low cost and tissue equivalence. However, these detectors are rarely used owing to the characteristics caused by low-atomic-number elements, such as low interaction coefficients and poor gamma-ray spectroscopy. In this study, we calculated the G(E) function to utilize a plastic scintillation detector in spectroscopic dosimetry applications. Numerous spectra with arbitrary energies of gamma rays and their H*(10) were calculated using Monte Carlo simulations and were used to obtain the G(E) function. We acquired three different types of G(E) functions using the least-square and first-order methods. The performances of the G(E) functions were compared with one another, including the conventional total counting method. The performance was evaluated using 133Ba, 137Cs, 152Eu, and 60Co radioisotopes in terms of the mean absolute percentage error between the predicted and true H*(10) values. In addition, we confirmed that the dose-rate prediction errors were within acceptable uncertainty ranges and that the energy responses to 137Cs of the G(E) function satisfied the criteria recommended by the International Commission.
김혜림(Hye Rim Kim),송성진(Seung Jin Song),김길영(Kilyoung Kim) 대한기계학회 2009 대한기계학회 춘추학술대회 Vol.2009 No.5
This study shows the theoretical analysis of surge dynamics in a turbo heat pump. The one-dimensional nonlinear model is developed. This is similar to the previous researches on unsteady analysis in simple compression system. But it is more complicated because the fluids in heat pumps undergo phase changes in heat exchangers and form closed-loop systems. So the two-phase flow effect and feedback effect from evaporator outlet to compressor inlet should be considered. The external conditions such as heat flux from cooling and chilled water are also important. Numerical results are presented to show the surge behavior in a turbo heat pump. It show that there are important parameters which determine the surge characteristics including Greitzer's B parameter and the other related to heat transfer in heat exchangers.