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Hormone Therapy in the Era of the COVID-19 Pandemic: A Review
( Sung Wook Choi ),( Juhee Kim ),( Jae Hoon Lee ),( Seul Ki Kim ),( Sa Ra Lee ),( Sung Hoon Kim ),( Hee Dong Chae ) 대한폐경학회 2022 대한폐경학회지 Vol.28 No.1
The coronavirus disease 2019 (COVID-19) pandemic has impacted the medical, social, and reproductive health of millions of people since its outbreak. The causative virus transmits, reproduces, and manifests through the respiratory tract. COVID-19 can invade any system of the body, including the cardiovascular and endocrine systems, through a secondary immune response. In particular, because the fatality rate is high in those over the age of 50 years, special attention is required during the medical care of this population. However, considering the benefit of therapy and the risk of COVID-19, high-quality evidence regarding individualized management in relation to hormone therapy is still insufficient in the field of gynecology. Furthermore, this review aims to serve as a reference for clinical application by analyzing and summarizing the results of studies reported to date regarding female hormone therapy in the context of the COVID-19 pandemic.
Inhibitory effect of Butanol fraction of Ecklonia cava on inflammatory mediators in RAW 264.7 cells
Chae, Hee-Sung,Choi, Jang-Ki,Kwon, Dong-Yeul The Korean Society of Oriental Pharmacy 2009 Journal of oriental pharmacy Vol.2 No.1
Objectives : The purpose of this study was to investigate the anti-inflammatory effects of Ecklonia cava butanol extract (BFEC) on RAW 264.7 cells. Method : To evaluate of anti-inflammatory of BFEC, We examined cytokine and Nitric oxide(NO) production in lipopolysacchride (LPS)-induced RAW 264.7 cell. Result : Extract of BFEC inhibit LPS-induced interleukin (IL)-6, NO production in human monocyte RAW 264.7 cells. Conclusion : BFEC down-regulated LPS-induced IL-6, NO production, which may be provide a clinical basis for anti-inflammatory properities of BFEC.
Chae, Hansong,Han, Seung Jung,Kim, Su-Young,Ki, Chang-Seok,Huh, Hee Jae,Yong, Dongeun,Koh, Won-Jung,Shin, Sung Jae American Society for Microbiology 2017 Journal of clinical microbiology Vol.55 No.9
<P>The prevalence of tuberculosis continues to be high, and nontuberculous mycobacterial (NTM) infection has also emerged worldwide. Moreover, differential and accurate identification of mycobacteria to the species or subspecies level is an unmet clinical need. Here, we developed a one-step multiplex PCR assay using whole-genome analysis and bioinformatics to identify novel molecular targets. The aims of this assay were to (i) discriminate between the Mycobacterium tuberculosis complex (MTBC) and NTM using rv0577 or RD750, (ii) differentiate M. tuberculosis (M. tuberculosis) from MTBC using RD9, (iii) selectively identify the widespread M. tuberculosis Beijing genotype by targeting mtbk_20680, and (iv) simultaneously detect five clinically important NTM (M. avium, M. intracellulare, M. abscessus, M. massiliense, and M. kansasii) by targeting IS1311, DT1, mass_3210, and mkan_rs12360. An initial evaluation of the multiplex PCR assay using reference strains demonstrated 100% specificity for the targeted Mycobacterium species. Analytical sensitivity ranged from 1 to 10 pg for extracted DNA and was 10(3) and 10(4) CFU for pure cultures and nonhomogenized artificial sputum cultures, respectively, of the targeted species. The accuracy of the multiplex PCR assay was further evaluated using 55 reference strains and 94 mycobacterial clinical isolates. Spoligotyping, multilocus sequence analysis, and a commercial real-time PCR assay were employed as standard assays to evaluate the multiplex PCR assay with clinical M. tuberculosis and NTM isolates. The PCR assay displayed 100% identification agreement with the standard assays. Our multiplex PCR assay is a simple, convenient, and reliable technique for differential identification of MTBC, M. tuberculosis, M. tuberculosis Beijing genotype, and major NTM species.</P>
Sestrin2 Attenuates Cellular Senescence by Inhibiting NADPH Oxidase 4 Expression
Chae Young Hwang,Ying-Hao Han,Seung-Min Lee,Sang-Mi Cho,Dae-Yeul Yu,Ki-Sun Kwon 대한노인병학회 2020 Annals of geriatric medicine and research Vol.24 No.4
Background: Sestrin2 (Sesn2) is involved in the maintenance of metabolic homeostasis and aging via modulation of the 5' AMP-activated protein kinase-mammalian target of rapamycin (AMPK-mTOR) pathway. Methods: Wild-type and Sesn2 knockout (KO) mice of the 129/SvJ background were maintained in a pathogen-free authorized facility under a 12-hour dark/light cycle at 20°C–22°C and 50%–60% humidity. Mouse embryonic fibroblasts (MEFs) were prepared from 13.5-day-old embryos derived from Sesn2-KO mice mated with each other. Results: The MEFs from Sesn2-KO mice showed enlarged and flattened morphologies and senescence-associated β-galactosidase activity, accompanied by an elevated level of reactive oxygen species. These senescence phenotypes recovered following treatment with N-acetyl-cysteine. Notably, the mRNA levels of NADPH oxidase 4 (NOX4) and transforming growth factor (TGF)-β were markedly increased in Sesn2-KO MEFs. Treatment of Sesn2-KO MEFs with the NOX inhibitor diphenyleneiodonium and the TGF-β inhibitor SB431542 restored cell growth inhibited by Sesn2-KO. Conclusion: Sesn2 attenuates cellular senescence via suppression of TGF-β- and NOX4-induced reactive oxygen species generation and subsequent inhibition of AMPK.