The impact of pathologic differentiation (well/ poorly) and the degree of Ki-67 index in patients with metastatic WHO grade 3 GEP-NECs.

Kim, Seung Tae,Lee, Su Jin,Lee, Jeeyun,Park, Joon Oh,Park, Young Suk,Lim, Ho Yeong,Kang, Won Ki Grune & Stratton 2017 Journal of clinical oncology Vol.35 No._suppl15

<P> e15686 </P><P> Background: Herein, we investigated the impact of pathologic differentiation (well or poorly differentiated) in metastatic grade 3 GEP-NEC patients receiving etoposide and platinum-based therapy. Simultaneously, we evaluated a more exact Ki67 index cut-off point to select patients with grade 3 GEP-NEC who might benefit from etoposide plus platinum (EP)-based therapy. Methods: Among patients pathologically diagnosed with metastatic grade 3 GEP-NECs at Samsung Medical Center between June 2013 and March 2016, 31 GEP-NEC patients receiving etoposide and platinum-based therapy were included in this study. Results: Primary sites included 13 foregut-derived GEP-NECs [stomach (n = 4), duodenum (n = 4), and pancreas (n = 5)] and 2 hindgut-derived GEP-NECs of the rectum. Sixteen unclassified GEP-NECs originated from 7 gall-bladder (GB), 6 liver and 3 unknown primary sites. According to pathologic differentiation, 14 patients had well differentiated and 17 had poorly differentiated grade 3 GEP-NECs. Between well differentiated and poorly differentiated grade 3 GEP-NECs, there was a significant difference in the distribution of Ki67 index. There was no significant difference of treatment efficacy between well and poorly differentiated grade 3 GEP-NECs (RR; 35.7% vs. 41.2%, p = 0.525). Tumor response to EP occurred in 5 of 7 patients with Ki67 > 60% and 7 of 24 with Ki67≤60%, which was significantly different (RR; 71.4% vs. 29.2%, P = 0.043). There was no significant difference in PFS according to pathologic differentiation (well differentiated vs. poorly differentiated) and Ki67 index ( > 60% vs ≤60%). Conclusions: Grade 3 GEP-NECs could be morphologically classified into well and poorly differentiated NETs. Additionally, among grade 3 GEP-NECs, there was a significant difference in ranges of Ki67 index between well and poorly differentiated NECs. Higher levels ( > 60%) of Ki67 index might be a predictive marker for efficacy of EP as a standard regimen in grade 3 GEP-NECs. </P>

인체 대장암 및 자궁경부암에서 PCR-SSCP법을 이용한 Ki-ras 암유전자의 점돌연변이에 관한 연구

박영홍,백낙환,김현찬,김상효,홍관희,김기태,이기영 인제대학교 1994 仁濟醫學 Vol.15 No.2

인체 대장암 20례와 자궁경부암 10례의 암조직을 대상으로 c-Ki-ras 유전자 codon 12와 13에서의 점돌연변이를 알아보고자 polymerase chain reaction(PCR)과 single-strand conformation polymorphism(SSCP) 검사법을 시행하였다. 대장암의 경우 20례중 9례(45%)에서, 자궁경부암의 경우 10례중 1례(10%)에서 양성으로 나왔다. 대장암에서는 codon 12에서 GGT가 TGT로의 치환이 4례로서 가장 많았으며, AGT, CGT로의 치환이 각 1례였고 codon 13에서는 GGC가 GAC로의 치환이 2례, TGC로의 치환이 1례인 것으로 나타났다. 자궁경부암의 경우 codon 12에서 GGT가 AGT로의 치환이 1례인 것으로 나타났다. In an attempt to clarify the role of genetic alteration in the genesis of human colorectal and cervical cancers, tissue specimens from 20 patients with colorectal cancer and 10 patients with cervical cancer were examined for the presence of point mutation in K-ras2 exon 1 by single strand conformation polymorphism analysis of PCR product. Exon I of c-Ki-ras2 was amplified by polymerase chain reaction(PCR) and comparison was made between the normal and mutated genes by nondenaturing polyacrylamide gel electrophoresis(PAGE) of PCR product and nucleotide sequence analysis using asymmetric PCR with direct sequencing. Genomic DNA from white blood cells were used as normal control and those from A427 cell line were used as mutated control. 1.PCR product from A427 cell line showed a distinct migration shift pattern compared to the normal control in PAGE and the direct sequencing indicated that nucleotide sequence of codon 12 was mutated from GGT to GAT. In addition, there was a loss of normal allele in A427 cell line. 2.PCR product from 9 cases(45%) out of a 20 colon cancer patients showed migration shifts in PAGE, and all of these 9 patients invariably demonstrated mutation of ras gene, either in codon 12(6 cases:30%) or 13(3 cases:15%). The base substitutions in codon 12 were: from GGT to AGT(1), CGT(1), or TGT(4). Base changes in codon 13 were from GGC to TGC(1) or GAC(2). 3.PCR product from 1 cases(10%) out of a 10 cervical cancer patients showed migration shrifts in PAGE, Invariably demonstrated mutation of ras gene in codon 12. The base substitutions was from GGT to AGT(1) TQE incidence of point mutation of c-Ki-ras in colon cancer was high, however, it was low in cervical cancer, which showed the relation of ruts gene mutation with colon cancer. PCR -SSCP analysis is a simple, rapid and efficient method of detection of point mutation, especially when dealing with multiple samples.

단일공법 복강경하 질식자궁절제술 110예의 고찰 및 다공법과의 비교

박병준 ( Byung Joon Park ),김용욱 ( Yong Wook Kim ),노덕영 ( Duck Yeong Ro ),김태응 ( Tae Eung Kim ),류기성 ( Ki Sung Ryu ),김장흡 ( Jang Heup Kim ) 대한산부인과학회 2010 Obstetrics & Gynecology Science Vol.53 No.7

목적: 통상적인 복강경기구만을 사용한 단일공법 복강경하 질식자궁절제술을 다공법 복강경하 질식자궁절제술과 비교하여 안전성 및 유용성을 평가하고자 하였다. 연구 방법: 2007년 4월부터 2009년 11월까지 인천성모병원에서 자궁근종 또는 자궁샘근증의 질환으로 단일공법 복강경하 질식 자궁절제술을 시행받은 환자 110명과 다공법 복강경하 질식자궁절제술을 시행받은 환자 110명, 총 220명의 환자를 대상으로 하였다. 우리는 모든 수술에서 통상적으로 사용되는 구부러지지 않는 일직선의 복강경기구를 사용하였다. 또한 단일공법 및 다공법 복강경하 질식자궁절제술 둘 다에서 수술자 (Kim, YW)의 이름을 따서 명명한 새로운 질단부 봉합 방법인 김씨 질구개 현수법 (Kim`s Vaginal Vault Suspension Method)을 사용하였다. 결과: 환자 나이, 수술 시간, 자궁의 무게, 혈색소 수치의 변화, 수혈 빈도, 수술 후 발열에서 두 군 간에 유의한 차이를 보이지 않았다. 환자들의 평균 나이는 46.7±7.0세(단일공법)와 45.5±6.3세(다공법)이었다. 평균 수술 시간은 87.2±21.0분(단일공법)과 83.3±20.3분(다공법), 평균 자궁의 무게는 261.4±139.7 g(단일공법)과 257.8±132.9 g(다공법), 평균 혈색소 수치의 변화는 1.1±0.7 g/dL(단일공법)와 1.2±0.6 g/dL(다공법)이었다. 두 군 모두에서 수술 중 장 또는 요로의 손상은 없었다. 두 군 모두에서 각각 한 건의 개복수술로의 전환이 있었다. 단일공법을 시행한 군에서 입원 기간이 유의하게 짧았다(2.6±0.6일 [단일공법], 3.3±0.7일 [다공법], P<0.05). 결론: 통상적으로 사용하는 구부러지지 않는 일직선의 복강경기구를 이용한 단일공법 복강경하 질식자궁절제술은 다공법 복강경하 질식자궁절제술에 대한 안전하고 유용한 대체 수술이 될 수 있다. Objective: To evaluate the safety and feasibility of single-port access laparoscopically assisted vaginal hysterectomy (SPA-LAVH) using conventional laparoscopic instruments compared to multi-port access laparoscopically assisted vaginal hysterectomy (MPA-LAVH). Methods: We reviewed the medical records of 220 patients with uterine leiomyoma or adenomyosis who underwent 110 SPA-LAVH and 110 MPA-LAVH in Incheon St. Mary`s Hospital between April 2007 and November 2009. We performed SPA-LAVH with conventional rigid straight laparoscopic instruments in all cases. We also performed a new vaginal cuff closure method, Kim`s Vaginal Vault Suspension Method, named after the operator (Kim, YW) in both SPA-LAVH and MPA-LAVH. Results: There was no significant difference in patients` age, operating time, uterine weight, hemoglobin change, frequency of blood transfusion, and incidence of postoperative fever between the two groups. The patients` mean age was 46.1±7.0 years (SPA-LAVH) and 45.5±6.3 years (MPA-LAVH). The mean operating time was 87.2±21.0 minutes (SPA-LAVH) and 83.3±20.3 minutes (MPA-LAVH). The mean uterine weight was 261.4±139.7 g (SPA-LAVH) and 257.8±132.9 g (MPA-LAVH). The mean hemoglobin change was 1.1±0.7 g/dL (SPA-LAVH) and 1.2±0.6 g/dL (MPA-LAVH). Neither bowel injury nor urinary tract injury occurred during the operation in the two groups. One of the SPA-LAVH and one of the MPA-LAVH cases were converted to abdominal total hysterectomy. The mean hospital stay time was shorter with SPA-LAVH (2.6±0.6 days [SPA-LAVH] and 3.3±0.7 days [MPA-LAVH], P<0.05). Conclusion: SPA-LAVH using conventional rigid straight laparoscopic instruments can be offered as a safe and feasible alternative to MPA-LAVH.

알루미늄 합금과 고장력 강판 접합을 위한 헬리컬 SPR의 단조공정 설계

김원영, 김동범, 김기호, 김도훈, 이인환, 조해용 충북대학교 산업과학기술연구소 2013 산업과학기술연구 논문집 Vol.27 No.2

A self-piercing rivet(SPR) is a mechanical component for joining dissimilar materials. It is used for joining dissimilar material sheets such as aluminum alloy and steel. The self-piercing rivet directly pierces into sheets without drilling sheets beforehand unlike conventional rivets. However, the self-piercing rivet occurs buckling when piercing high strength steel. Therefore, a helical SPR which can pierce high strength steel sheet was designed. In this study, forging process was designed for manufacturing the helical SPR and simulated by using commercial FEM code DEFORM-3D. In the simulation of forging process for manufacturing rivet, process sequence, formability, forging load were investigated. The suitable forging process could be designed by comparisons of simulated results. Experimental trials have been carried out for same conditions as simulated ones. The simulated results agreed with the experimental ones. So, the analytic approach in this thesis is useful to save cost and time in the forging industry

Soccer에 있어서 敏捷性 Training에 關한 一硏究

金永煥,金基學 慶北大學校 師範大學 1973 敎育硏究誌 Vol.15 No.-

For the soccer, physical energy is the essential foundation in utilizing the skills and tactics in the field, the more efficient the physical ability is, the better the training in the skills and tactics is in quality as well as quantity. To develop the ability to get the higher scores at the goalshooting, it is necessary for the team to be given the Speed Training such as runing faster than the opponents, the better and quicker control and seizing of the ball. To achive thus goals We should train the team as the following, (1) Motors of the track and field Charateristics. (2) Motors of gymnastics Charateristics. (3) Motors using the ball. (4) Motors with the Characteristics of the game.

이미지 개선 및 선 정보를 이용한 자동차 번호판 추출

김기영,최영우 숙명여자대학교 자연과학연구소 2000 자연과학논문집 Vol.- No.11

본 논문은 실외 도로에서 획득한 낮은 질의 차량 영상에서 이미지를 개선하고 선 정보를 이용하여 자동차 번호판을 추출하는 방법을 제안한다. 이미지의 질을 개선하기 위해서 명암 대비를 이용한 스트레칭 방법과 수정된 Niblack의 이진화 방법을 사용하여 전처리를 수행한다. 선 정보추출은 기존에 많이 사용된 허프 변환이 시간과 메모리 소모의 단점이 있으므로, 이진화한 영상에서 에지를 추출하여 만든 런(Run)의 연결 요소를 분석하여 선으로 병합하는 방법을 제안한다. 추출된 선 가운데 수직선을 기준으로 후보 영역을 결정하며, 번호판 영역의 명암 변화도 값, 명암 변화도 패턴 및 구조적인 정보를 이용하여 검증한다. 제안한 방법을 적용한 결과 불규칙한 조명이나 노면 상태 등에서 획득한 낮은 질의 이미지에 대해서도 50%이상의 번호판 추출률을 보였다. This paper propose a method fur extracting license plate areas from low quality vehicle images using image enhancement and line information. To improve the quality of the images we apply an improved histogram stretching method and a modified Niblack's binariBation method to the images in the preprocessing stage. And the lines are extracted from the image by combining multiple runs of horizontal and vertical edges instead of using computation intensive Hough transforms. Candidate areas of the licence plate are assumed by combining pairs of the vortical lines first and then by composing rectangular areas in the image. The candidate areas are verified by analyzing gray level variations and their patterns with plate's structural information. We have applied the proposed method to the various kinds of the low quality vehicle images and obtained the extraction rate of more than 50%.

體育活動을 通한 새마을 運動의 成果 擧揚 方案 : 새마을 農民體操 創案을 中心으로 Centering around creative Saemaul Farmer's Exercises

金正默,金基學,申榮吉 慶北大學校 師範大學 1979 敎育硏究誌 Vol.21 No.-

The research is pertinent to the actual health conditions of farmers in this country. Among the diseases from which they suffer are those involving stomach, spinal column, shoulders, and the lower limbs. For that matter, the Saemaul Farmer's Exercises are made up with the goal of refreshment, preventive correction and distraction as well. Futhermore, the exercises of the total body aim at making progress in physical strength. The exercises consist of thirteen different motion; the movements are done in two-dimension, these exercises are for both begginers and for skillful people. The frequency of repeating motions becomes different according to sex, and physical strength. Ustally sixteen respiratory intervals are for the weak, the old, and females; thirty-two respiratory intervals are used by the strong and young, predominatly by male. The exercises themselves have flexibility which makes it possible for individuals to increase or reduce the amount of motion. There are thirteen kinds of movements in the following order: 1) Stretching up 2) Bending and Swinging neck 3) Twisting and Revolving Arms 4) Widening, Bending, and Straightening Limbs 5) Stretching Arms while Tightening Chest Muscular 6) Side Stretching 7) Stretching Body Back and Forth 8) Twisting from side to Side 9) Lifting Thighs in turns and Turning Waist Over 10) Twisting and Stretching Body Back and Forth 11) Jumping on Tiptoe and Hopping 12) Lifting heels and Putting Weight of Body on Toes 13) Controlling Breath, both Inhaling and exhaling

이기종 운영체계에 기반한 고성능 트랜잭션 처리 시스템의 개발

김기형,남영환,조행래 嶺南大學校 工業技術硏究所 1998 工業技術硏究所論文集 Vol.26 No.2

Recently, workstation clusters have been utilized for high performance transaction processing systems. In such systems, transactions are usually processed by multi-threads. Workstations in the clusters can have heterogeneous operating systems in the viewpoint of the thread processing mechanism. This paper focuses on the portability issues which should be considered from the design stage of the workstation cluster-based transaction processing systems. Especially, this paper analyzes the differences between single-threaded and multi-threaded operating systems. Based on this analysis this paper presents the architecture of a high performance transaction processing system which works on both operating systems.

미래사회에 요구되는 교육적 인간상 : 그 성격과 과제

김기민,전영곤 昌原大學校 學生生活硏究所 1996 학생생활연구 Vol.9 No.-

동결속도제어에 의한 동결건조기 성능개선 및 동결건조품의 물리적 특성에 관한 연구

김정한,신영우,김민용,박기원,강정길 여수대학교 1998 論文集 Vol.12 No.2

It is essential to establish optimum operating condition in order to decrease freeze drying time and energy cost on freez-drying process. In this study freezing point, thermal condutivity and permeability of vapour on freeze-drying process of coffee solution were measured. The results of abtained were summarized as follows: 1) thermal conductivity were not affected by freezing rate but were increased with increasing concentration of coffee solution 2) permeability of vapour was increased with decreasing freezing rate and concentration of coffee solution These result suggested that freezing temperature of freeze-drying material must be determined through measuring freezing point of material for the purpose of saving energy cost and ahortening freezing time 1) thermal conductivity depend on solids porosity and was not concerned with size and shape of pore 2) permiability decrease with lowering freezing temperature beacuse radius of freeze drying layer was smaller and permiability decrease with higher concentration because of porosity was decreased