Cloning and characterization of a novel gene with alternative splicing in murine mesenchymal stem cell Line C3H/10T1/2 by gene trap screening

( Ming Ke Wang ),( Hui Qin Sun ),( Fan Jiang ),( Jing Han ),( Feng Ye ),( Tao Wang ),( Yong Ping Su ),( Zhong Min Zou ) 생화학분자생물학회 (구 한국생화학분자생물학회) 2010 BMB Reports Vol.43 No.12

A novel gene, designated mgt-6, containing four splicing variants, was isolated from a gene trap clone library of C3H/10T1/ 2 cells transfected with retroviral promoterless gene-trap vector, ROSAFARY. The transcript variants were differentially expressed in murine tissues and cell lines and differentially responded to diverse stimuli including TGF-β1 and mitogen-activated protein kinase (MAPK) inhibitors. The mgt-6 gene encoded a protein of 37 or 11 amino acid residuals with cytoplasmic distribution. However, when C3H/10T1/2 cells were treated with 5-azacytidine, the protein translocated into cell nucleus as indicated by fused LacZ or C-terminally tagged EGFP. Our preliminary results suggest that further study on the role of mgt-6 gene in cell transformation and differentiation may be of significance. [BMB reports 2010; 43(12): 789-794]

Depth Distributions of Bi+ Ions Implanted into Ni, Si and SiO₂, Films

Ke-Ming Wang,Feng Chen,Xue-Lin Wang,Jian-Hua Zhang,Xiang-Dong Liu 한국진공학회(ASCT) 2002 Journal of Korean Vacuum Science & Technology Vol.6 No.1

Ni, Si and SiO₂ films were implanted by 350 keV Bi^+ ions at room temperature with fluences of 1×10^(16) and 2×10^(16) ions/㎤. The depth distributions of implanted Bi^+ ions in Ni, Si and SiO₂ films were by investigated by Rutherford backscattering. The results show that the depth distributions of implanted Bi^+ ions into Ni, Si and SiO₂ films have obeyed nearly Gaussian distributions. The maximum difference between experimental and calculated values is less than 18 % for mean projected range. Experimental range straggling deviated significantly from calculated value. The possible reasons are discussed.

Characteristics of Refractive Index Profiles at Different Temperatures in LiNbO₃ and KTiOPO₄ Waveguide Formed by 350 keV Light Ions

Ke-Ming Wang,Feng Chen,Hui Hu,Hui-Hao Xia,Xue-Lin Wang,Bo-Rong Shi,Qing-Ming Lu 한국진공학회(ASCT) 2003 Applied Science and Convergence Technology Vol.12 No.S1

Both LiNbO₃ and KTiOPO₄ samples were implanted with 350 keV H^+ and He^+ ions at different doses ranging from 1×10^(16) to of 5×10^(16) ions/㎠. Single and multi-energy implantations were performed at room temperature. Mono-mode or a few modes in both LiNbO₃ and KTiOPO₄ waveguides were observed. The effect of temperature on the refractive index profiles of LiNbO₃ and KTiOPO₄ waveguids was studied. The temperature covered from room temperature, 200℃, 194.5 K (dry ice) and 77K (liquid nitrogen). Different mechanisms are needed to interpret the observed behavior. A n_e increased mono-mode LiNbO₃ waveguide was formed by multi-energy keV He^+ ions.

游牧者的?? : 匈奴?卑?西羌游牧社?及其??帝?的互?

王明珂(Wang Ming-Ke) 중국고중세사학회 2007 중국고중세사연구 Vol.18 No.-

유목이란 농업 자원이 극도로 부족한 환경에 적응하기 위한 수단의 하나이지만, 자급자족할 수가 없기 때문에 다른 생계활동에 종사함으로써 생존에 필요한 물자를 얻는다. 흉노부락의 유목은 광대한 몽고초원에서 이루어졌다. 이곳에서의 유목 생계는 매우 비슷하였다. 초원에는 아무런 지리적 장애물이 없었기 때문에 만약 서로 약탈해서 자원부족을 해결하려고 한다면 피차 쓰러질 때까지 싸우는 수밖에 없었다. 따라서 초기 초원유목부족들은 집결해서 '국가’를 형성하고, 대내적으로는 초원을 분배하여 부락 간의 평화와 안정을 유지하였으며, 대외적으로는 경제 영역을 중국, 서역, 鳥桓, 丁令 등지까지 확장했다. 국가의 역량으로 무역노선을 보호하였고, 무력으로 정주 취락에게서 징세를 하였으며, 전쟁과 약탈을 통해 외부 자원을 얻었다. 西羌이 유목을 했던 곳은 河湟지역인데, 이곳의 환경은 몽고초원과 크게 다르다. 하황지구는 본래 유라시아 초원과 화북평원과 격리되어 있고, 그 내부는 높은 산과 계곡으로 작은 구역으로 분할되어 있다. 서강 부락은 대외적으로 먼 곳에서 생활 자원을 얻기 어려웠지만, 산과 계곡의 자원을 잘 이용해 생존에 크게 문제는 없었다. 이곳에서의 유목과 기타 자원은 산과 계곡에서 얻어야 했으므로 서로 약탈이 끊이지 않았다. 따라서 부락간의 정치적 결합이 어려웠다. 오환과 선비가 활동했던 西遼河지역과 대흥안령 남쪽 지역은 환경상 삼림에서 초원까지 다양하다. 삼림초원 지구에서 유목하였던 부족은 주변의 흉노와 화하 등 항상 다른 경제형태의 부족과 접촉하였는데 이 때문에 부락연맹을 결성하여 대내적으로는 부락간의 자원충돌을 줄이고 대외적으로는 무역과 약탈을 해나갔다. 오환과 선비는 서강에 비해 정치의 중앙화를 갖출 수 있었기 때문에 대외적으로 자원을 확장해 나가는 목표를 이룰 수 있었다. 진한시대부터 위진시대까지 약 500년간 화하제국의 자원 경계선 바깥에 놓여 있던 북방 유목민들은 각기 자발적으로 전문화된 유목 경제를 발전시켰으며 그에 맞추어 사회조직이 발전하였다. 그러나 華夏의 자원봉쇄 혹은 확장 시에 흉노는 국가로서, 오환과 선비는 부락연맹으로서, 서강은 부락 및 단기간의 부락연맹으로서 각각 화하제국과 대응하였다. 이후 청말까지 1500년간 세 종류의 화하 북방 경계지역의 상황은 계속 이어졌다.

ppGalNAc T1 as a Potential Novel Marker for Human Bladder Cancer

Ding, Ming-Xia,Wang, Hai-Feng,Wang, Jian-Song,Zhan, Hui,Zuo, Yi-Gang,Yang, De-Lin,Liu, Jing-Yu,Wang, Wei,Ke, Chang-Xing,Yan, Ru-Ping Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.11

Objectives: To investigate the effect of glycopeptide-preferring polypeptide GalNAc transferase 1 (ppGalNAc T1 ) targeted RNA interference (RNAi) on the growth and migration of human bladder carcinoma EJ cells in vitro and in vivo. Methods: DNA microarray assays were performed to determine ppGalNAc Ts(ppGalNAc T1-9) expression in human bladder cancer and normal bladder tissues. We transfected the EJ bladder cancer cell line with well-designed ppGalNAc T1 siRNA. Boyden chamber and Wound healing assays were used to investigate changes of shppGalNAc T1-EJ cell migration. Proliferation of shppGalNAc T1-EJ cells in vitro was assessed using [3H]-thymidine incorporation assay and soft agar colony formation assays. Subcutaneous bladder tumors in BALB/c nude mice were induced by inoculation of shppGalNAc T1-EJ cells and after inoculation diameters of tumors were measured every 5 days to determine gross tumor volumes. Results: ppGalNAc T1 mRNA in bladder cancer tissues was 11.2-fold higher than in normal bladder tissues. When ppGalNAc T1 expression in EJ cells was knocked down through transfection by pSUPER-shppGalNAc T1 vector, markedly reduced incorporation of [3H]-thymidine into DNA of EJ cells was observed at all time points compared with the empty vector transfected control cells. However, ppGalNAc T1 knockdown did not significantly inhibited cell migration (only 12.3%). Silenced ppGalNAc T1 expression significantly inhibited subcutaneous tumor growth compared with the control groups injected with empty vector transfected control cells. At the end of observation course (40 days), the inhibitory rate of cancerous growth for ppGalNAc T1 knockdown was 52.5%. Conclusion: ppGalNAc T1 might be a potential novel marker for human bladder cancer. Although ppGalNAc T1 knockdown caused no remarkable change in cell migration, silenced expression significantly inhibited proliferation and tumor growth of the bladder cancer EJ cell line.

Annealing Characteristics of Planar Waveguides in Lanthanum Gallium Silicate Crystal by Oxygen Implantation

Xue-Lin Wang,Feng Chen,Lei Wang,Ke-Ming Wang 한국물리학회 2008 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.52 No.-

Planar waveguides have been fabricated in z-cut lanthanum gallium silicate (LGS) crystal by 6.0 MeV oxygen-ionsimplantation with a dose of 6×10^15 ions/cm². The waveguides were characterized by the prism-coupling method. The dark modes are measured before and after annealing at 600, 700, 750 and 800℃ for different times in air. The refractive index profiles are reconstructed by using a reflectivity calculation method. TRIM'98 code is used to simulate the process of the oxygen-ion implantation into LGS. It is found that the refractive-index change may be partly due to the damage induced by nuclear collision, and the waveguide has thermal stability below 600℃. Planar waveguides have been fabricated in z-cut lanthanum gallium silicate (LGS) crystal by 6.0 MeV oxygen-ionsimplantation with a dose of 6×10^15 ions/cm². The waveguides were characterized by the prism-coupling method. The dark modes are measured before and after annealing at 600, 700, 750 and 800℃ for different times in air. The refractive index profiles are reconstructed by using a reflectivity calculation method. TRIM'98 code is used to simulate the process of the oxygen-ion implantation into LGS. It is found that the refractive-index change may be partly due to the damage induced by nuclear collision, and the waveguide has thermal stability below 600℃.

A new species of gall midge (Diptera: Cecidomyiidae) damaging flower buds of goji berry Lycium barbarum (Solanaceae)

Ke-Long Jiao,Xiao-Yi Zhou,Hai-Li Qiao,Hao Wang,Jun Chen,Bai-Ming Liu,Wen-Jun Bu,Peter Kolesik 한국응용곤충학회 2020 Journal of Asia-Pacific Entomology Vol.23 No.4

Larvae of a previously undescribed gall midge species have been feeding within malformed flower buds of goji berry Lycium barbarum L. (Solanaceae) in China, preventing fruit development and causing damage to the fruit industry. The new species is named Gephyraulus lycantha Jiao & Kolesik, its morphology is described, the COI mitochondrial gene segment is sequenced, and the biology is outlined. In order to taxonomically accommodate the new gall midge, the scope of the genus Gephyraulus is broadened and now encompasses also species that lack dorso-apical sensoria on the female cercus.

Peptidoglycans Promotes Human Leukemic THP-1 Cell Apoptosis and Differentiation

Wang, Di,Xiao, Pei-Ling,Duan, Hua-Xin,Zhou, Ming,Liu, Jin,Li, Wei,Luo, Ke-Lin,Chen, Jian-Jun,Hu, Jin-Yue Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.12

The innate immune system coordinates the inflammatory response to pathogens. To do so, its cells must discriminate self from non-self utilizing receptors that identify molecules synthesized exclusively by microbes. Toll-like receptors have a crucial role in the detection of microbial infection in mammals and insects. In mammals, they have evolved to recognize conserved products unique to microbial metabolism. These include lipopolysaccharide (LPS), lipotechoic acids, and peptidoglycans (PGN). We show here that TLRs, including TLR2, are expressed on the THP-1 human leukemia cell line. Activation of TLR2 signaling in THP-1 by PGN induces the synthesis of various soluble factors and proteins including interleukin-$1{\beta}$, interleukin-8 and TNF-${\alpha}$ and apoptosis of THP-1 with PGN dose and time dependence. Moreover, in this study we show that PGN induces apoptosis of THP-1 cells in a TNF-${\alpha}$-dependent manner. These findings indicate that TLR2 signaling results in a cascade leading to tumor apoptosis and differentiation, which may suggest new clinical prospects using TLR2 agonists as cytotoxic agents in certain cancers.

Preparation of Copper Sulfide by Microwave Assisted Method and Its Application on Superhydrophobic, Anti-UV and Photocatalytic Cotton Fabric

Ke-Cheng Yang,Li-Hui Xu,Hong Pan,Li-Ming Wang,Yong Shen,Ying Ding 한국섬유공학회 2020 Fibers and polymers Vol.21 No.6

In this work, solid spherical and hollow spherical CuS were prepared by microwave assisted method withPEG4000 as dispersant. The CuS was characterized by particle size analysis, scanning electron microscope(SEM), nitrogenadsorption and desorption, and X-ray diffraction (XRD), respectively. The effects of PEG4000, ratio of thiourea: copper, andmicrowave power on particle size of CuS were discussed, and the effects of particle size on photocatalytic property wereevaluated. The solid spherical CuS had average particle size of 189.1 nm and specific surface of 9.54 m2/g. The hollowspherical CuS had average particle size of 502.7 nm and specific surface area of 16.01 m2/g. The results showed the hollowspherical CuS had better photocatalytic property. In addition, the cotton fabric was treated by hollow spherical CuS/polydimethylsiloxane (PDMS) to prepare multifunctional textile in this work. The treated cotton fabric was characterized bySEM. The treated cotton fabric achieved the superhydrophobicity with contact angle of 156.9 o and sliding angle of 8 o, goodphotocatalytic properties for methylene blue, and exceptional UV resistance with UPF of 223.52.

Preparation of CuS/SiO2 Composite Modified Aerogel and Its Superoleophobic and Photocatalytic Properties

Ke-Cheng Yang,Li-Hui Xu,Hong Pan,Li-Ming Wang,Yong Shen,Ying Ding 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2020 NANO Vol.15 No.03

In this study, CuS/SiO2 composite modified aerogel was prepared by the incorporation of hollow spherical CuS into methyltrimethoxysilane-based SiO2 sol and modification with hexadeca-fluorodecyltriethoxysilane via acid-base catalyzed sol–gel reaction and drying under ambient pressure. The CuS/SiO2 composite modified aerogel was characterized by Fourier-transform infrared (FT-IR) spectrometry, scanning electron microscope (SEM), nitrogen gas adsorption and desorption and X-ray diffraction (XRD), respectively. The effects of CuS and fluorosilane concentration on density and porosity of aerogel, oleophobic and photocatalytic properties were evaluated. The results showed that structure and physical properties of aerogel had some effect by introducing CuS and fluorosilane, and the CuS/SiO2 composite modified aerogel with density of 0.146 g/cm3 and specific surface area of 241 m2/g achieved super-oleophobicity with oil contact angle of 152.8fl and sliding angle of 10fl, and good photocatalytic properties for methylene blue.