수부 주상골 요부의 전위골절에 대한 수술적 치료

조용만,이영기,강흥식,리건영,이천동,오상수 대한골절학회 1997 대한골절학회지 Vol.10 No.1

The authors surveyed the clinical study of 17cases of scaphoid fractures from March 1989 to September 1994 with minimal I year follow up at Department of Orthopedic Surgery, Lee-Rha general Hospital. The following conclusions were obtained through clinical survey. l. Of all l 7 cases, they were l3 males and 4 females; Among the 13 males, 10 cases were aged between 20 and 39 years. forming 56.8% of all. 2. All 17 cases were treated by open reduction. Unions were obtained at average l l.5 weeks in 7 cases treated with Herbert screw fixation and l 2 weeks in l 0 cases treated with K-wire fixation. 3. Two of ten K-wire treated cases resulted in delayed union, one of seven Herbert screw treated cases resulted in delayed union. 4. Unions were obtained at average 20 weeks in 3 cases of delayed union. We concluded that open reduction and K-wire fixation were recomanded for the compatible treatment method of displaced waist fracture of scaphoid.

톨루엔 노출 근로자에서 유전자적 요인, 생활습관 및 식이가 요중 마뇨산 배설에 미치는 영향

임현우,박정일,노영만,이강숙,이정민,이원철,이세훈,정치경 大韓産業醫學會 2000 대한직업환경의학회지 Vol.12 No.3

목 적 : 본 연구는 생물학적 지표 특성 연구를 위한 단면조사 연구로서 톨루엔 노출과 톨루엔의 생물학적 모니터링으로 이용되는 요중 마뇨산 농도간의 양-반응 관계에서 효과 변경인자로서 작용하는 유전자 ALDH2, CYPIAI, CYP2El의 다양성 분포를 조사하고, 이들 유전자가 요중 마뇨산 배설 농도에 미치는 영향을 알아보고자 하였다. 방 법 : 연구대상자는 경인지역에 위치한 22개의 사업체에서 페인트 제조 72명, 제조업에서 도장작업 50명, 인쇄업 12명, 접착작업 12명, 벽지의 코팅작업 14명등 160명의 근로자이었다. 성, 연령, 유기용제 작업장 근무경력, 흡연습관, 음주습관, 검사일 이전 밤에 음주 여부, 시료 채취전 6시간 이내에 섭취한 식품조사, 측정일 개인보호구의 착용 여부에 대하여 설문조사를 시행하였다. 톨루엔에 대한 개인노출량은 passive air sampler로 측정하였고, 요중 마뇨산은 요중 크레아틴으로 보정하여 측정하였다. 또한 aldehyde dehydrogenase 2 (ALDH2) , cytochrome P45O IAI(CYPIAI), 그리고 cytochrome P45O 2El(CYPfEl)의 유전적 다양성은 혈액으로부터 백혈구의 DNA를 추출하여 제한 효소 절단 길이 다양성 (restiction fragment length polymorphism, RFLP)법으로 검사하여 다음의 결과를 얻었다. 결 과 : 연구대상자의 요중 마뇨산 농도의 기하평균은 0.44g/g creatinine, 기하표준편차 2.80이었다. 톨루엔 노출 농도, 개인 보호구 착용여부, 안식향산이 함유된 식품의 섭취여부중 요중 마뇨산 배설농도에 영향을 미치는 인자는 톨루엔 노출 농도뿐이었다. ALDH2, CYPIAI, CYPfEl, 연령, 흡연, 근무기간에서 회귀계수간에 차이를 보이는 경향이 있었다. 요중 마뇨산 농도에 대한 다중회귀분석에서 톨루엔, ALDH2, CYPIAI, CYP2El유전자형의 회귀계수가 통계적으로 유의하였다. 결 론 : 톨루엔 노출로 인한 요중 마뇨산 배설 농도는 ALDH2, CYPIAI, CYP2El유전자 다양성에 의해 영향을 받기 때문에, 유전자형의 대사능의 차이를 고려하여 마뇨산 기준 농도를 찾는 연구가 이루어져야 하겠다. Objectives The purpose was to investigate the distributions and the effects of genetic polymorphism of aldehyde dehydrogenase 2(ALDH2), cytochrome P45O IAI (CYPIAI), and cytochrome P45O 2El (CYPfEl) on the toluene metabolism. Methods : The subacts consisted of 160 workers who were exposed to toluene in different industries such as paint manufacturing, painting on steel and wood products, printing, bonding, and coating. The exposed toluene level was monitored by passive air sam paler, and the questionnaire variables were age, sex, smoking, drinking, previous night's drinking, use of personal protectlve equipment, work duration, and taking benzoic acid containing food. The urinary hippurric acid collected in the end of shift was corrected by urinary creatinine concentration. The genotypes of ALDH2, CYPIAI, and CYP2El were investigated using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) methods with DNA extracted from venous blood. Results'The geometric mean and the geometric standard deviation of urinary hippuric acid concentration were 0.44 g/g creatinine and 2.80. The urinary hippuric acid concentration was significantly related to personal exposed toluene level among personal exposed toluene level, use of personal protective equipment, and benzoic acid containing food diet. The slope differences of the regression for ALDH2, CYPIAI, and CYP2E1 genetic polymorphism, age, smoking, and work duration tended to be significant. In multiple regression analysis, the regression coefficient of toluene, ALDH2, 7YPIAl, CYP2E1 genetic polymorphism were significant. Conclusions : From the above results, urinary hippuric acid level after toluene exposure was significantly affected by the genetic polymorphism of ALDH2, CYPIAI, CYPfEl. It is needed further investigation of the urinary hippuric acid level considering the effect of genetic polymorphism.

톨루엔 폭로 작업자에서 Aldehyde dehydrogenase2(ALDH2)의 유전자형과 요중 마뇨산

이강숙,노영만,박정일,허성옥 가톨릭대학 산업의학 센타 산업의학연구소 1995 韓國의 産業醫學 Vol.34 No.2

This study was conducted to evaluate the distribution and the effect to urinary hippuric acid excretion according to the polymorphism of aldehyde dehydrogenase2(ALDH2) which is major role in oxidaton of benzaldehyde to benzoic acid. For 39 workers exposed to toluene and 42 white collar workers, we extracted the DNA from venouse blood and confirmed the genotype of aldehyde dehydrogenase2 using the polymerase chain reaction(PCR) and artificial created restriction site method. In workers exposed to toluene, normal (NN) type were 34 (88%) and inactive(ND) type were (12%), but in white collar workers NN type were 27(65%) and ND type were 14(33%), DD type were 1(2%). It showed the significant lower rate of inactive type of ALDH2 in workers exposed to toluene than white collar workers. The results of workplace toluene measurement were below 20ppm. For urinary hippuric acid there was not significant differnce between normal and inactive type of ALDH2. It suggested that further evaluation should be conducted in high concentration of toluene exposure group.

생쥐 2-세포기 수정란의 초급속동결

강만종,이철상,한용만,유대열,이경광 제주대학교 농과대학 제주도축산문제연구소 1991 畜産論叢 Vol.6 No.1

This study was carried out in order to investigate eggects of cryoprotectant concentration and equilibration time on survival of ultrarapidly frozen 2-cell mouse embryos Mouse 2-cell embryos. fol-lowing dehydration by exposure to DMSO and sucrose. were directly immersed into liquid nitrogen and thawed in 37℃ water. Viability was defined by development rate to the blastocyst stage after in vitro culture for 72 hours. The results are summarized as follows ; 1. When 0.25M of sucrose was added into the freezing medium a t various concentrations of DMSO and dilution medium, higher development rate of embryo was obtained in 3.0M DMSO conentrations (82.6%). However, When sucrose concentrations of 0.25 and 0.5M were added to the freezing medium with 3.0M DMSO and dilution medium, development rate of embryos were 81.7% and 24.1%, respectively. 2. In the equilibration time at room temperature, higher development rate was attained after short period of time(2.5min) in 3.0M DMSO +0.25M sucrose(85.9%). 3. The development rate of embryos at in vitro 2-celL in vivo 2-celL solution control and untreated control was 84.6%, 90.9%, 89.9%. and 89.7%. respectively.

初乳의 分泌期間中의 成分變化 및 保存性에 관한 硏究

李鳳求,金相根,李晩徽,金武剛 충남대학교 수의과대학 동물의과학연구소 1995 動物醫科學硏究誌 Vol.3 No.-

The study was carried out to investigate the charges of hourly and daily components and preservation of colostrum produced 1st through 7th day after parturition of Holstein cows. The results obtained were summarized as follows : 1. The specific gravity in colostrum was 1.032-1.057 from 1 to 24 hrs after parturition and then showed slightly higher than normal milk. The pH and titerable acidity were 6.43 and 0.170% at 1 hrs after parturition respectively and thereater gradually increased. 2. The solids not-fat, fat and protein of colostrum were 16.24%, 6.60% and 14.20% at 1hrs after parturition respectively, and then showed markedly higher than normal milk. 3. The solids not-fat, fat and protein of colostrum were 9.02%, 6.66% and 3.92% at day 1 postpartum respectively and thereafter gradually decreased. But milk lactose ranged from 2.47% to 4.76% at day 1-7 postpartum and thereafter gradually increased. 4. The F, Mg and Fe of colostrum were 0.244%, 0.064% and 0.430% at day 1 postpartum respectively. The Ca and Na were 0.262% and 0.074% at day 1 postpartum and then showed markedly higher than normal milk and thereafter gradually decreased. 5. The pH from 1st through 5th days of milking was decreased from 6.4 to 5.3. The number of common bacteria reached a peak levels of 1×10^7.5/ml after 3-7 days and then slightly decreased. The number of coli from group reached a peak levels of 1×10^8/ml after 3 days and thereafter rapidly decreased to 10^3/ml after 20 days. 6. The number of common bacteria of colostrum added 0.5, 1.0 and 2.0% of propionic acid and acetic acid at 20℃ were inhibited from 1 to 20 days in addition of 0.5%-2% propionic acid and acetic acid. The number of coli form group was retarded from 3 to 7 days addition of 1.0%-2.0% propionic acid and acetic acid. The addition of propionic acid and acetic acid to colostrum were effective in retarding the growth of bacteria than acetic acid.

다발성 외상시 응급 초음파검사의 의의

이강현,임경수,김선만,김영식,황성오,유수영,강성준 대한응급의학회 1995 대한응급의학회지 Vol.6 No.1

The Evaluation of blunt abdominal trauma is clinically challenging. Abdominal computed to-mography(CT) and diagnostic peritoneal lavage(DPL) has become primary diagnostic modali-ty in advanced trauma life support(ATLS). But DPL is invasive study with potential for the patients. Monitoring and resuscitation are usually suboptimal during there examinations. We examined the efficacy and role of emergency ultrasonographic(US) studies in the initial abdominal evaluation of blunt trauma patients. A prospective study was carried out on 101 blunt trauma patients from 1994, June to September. 101 patients underwent real-time US ex-amination of the chest and abdomen. Indication for emergency US included the cases of shock (systolic blood pressure<90mmHg) or tachycardia (pulse rate/min.>100) in blunt trauma pa-tients. US showed a sensitivity of 96.2%, specificity 98%, accuracy 97% in regard to indica-tion for surgery in case of blunt abdominal or thoracic trauma. US saves time, and it can be performed during the emergency care in the emergency department. US showes high sensitivity and specificty, and is the method of first choice in the evaluation of blunt trauma.

Brevibacterium erythrogenes에 의한 스테롤로부터 1,4-Androstadiene-3,17-dione 생성

이은아,이강만 梨花女子大學校 藥學硏究所 1991 藥學硏究論文集 Vol.- No.1

한국의 토양으로부터 17-ketosteroid인 ADD를 생성하는 미생물을 분리하여 동정하고 배지조성 및 발효조건을 검토하였다. 집적배양법을 통하여 효소저해제 첨가법에 의해 ADD를 생성할 수 있는 미생물을 분리, 선별하여 9-15라 명명하였다. 이 균주의 형태학적, 생리학적, 생화학적 특성 및 DNA의 T_m을 측정한 결과 Brevibacterium erythrogenes로 동정하였다. ADD 생성의 최적 배지조건 및 발효조건을 검토한 결과 0.1% 콜레스테롤을 함유하는 발효배지에 lactose 0.2%, beef extract 0.2%, bentonite 0.5%를 첨가하고 pH를 7.4로 맞추어 멸균하여 배양 후 18시간에 효소저해제인 α, α´-dipyridyl(1 mM)을 첨가하고 발효시키는 것이 최적 조건임이 검토되었다. 이 조건에 따라 ADD생성을 검토한 결과 27.3%의 전환수율을 얻었으며, 한편 대조실험인 pH7의 0.1% cholesterol 발효배지를 사용하고 배양 후 20시간에 α, α´-dipyridyl을 가하는 배지 및 발효조건으로 배양한 경우는 18.5%의 전환수율을 나타내었다. Microbiological conversion of sterols to 17-ketosteroids has been recongnized as a source for commercal preparation of steroidal drugs. For the purpose of strain development, we isolated microorganisms through enrichment culture method and identified an isolate strain. The strain was closely related to Brevibacterium erythrogenes. The optimal conditions for 1,4-androstadiene-3,17-dione(ADD) formation were as follows; pH 7.4, lactose 0.2%, beef extract 0.2%, bentonite 0.5% in the chlolesterol fermentation medium. Maximum production was obtained with the addition of α, α'-dipyridyl (1 mM, final conc.) at 17-20 hours after incubation.

부안 유천리 도자기의 과학적 분석

강현삼,이한형,박기철,김건한,서만철,서정호,최기영 공주대학교 문화재보존과학연구소 2003 문화재과학기술 Vol.2 No.1

전북 부안 유천리 청자요지에서 수습된 청자편 11점의 태토 및 유약에 대하여 화학조성을 분석하고, 기 발표되어진 문헌의 청자데이터들을 종합하여, 이와 비교함으로써 본 시료의 도자특성과 및 산지에 대한 정보를 유추하였다. 태토의 주요산화물 분석에는 XRF를 이용하였으며, 유약의 주요산화물분석에는 EPMA를 이용하였다. 태토 중 17개 미량성분의 분석에는 ICP-MS를 이용하였고, 자료의 비교분석에는 Seger식에 의한 방법과 주성분분석법에 의한 통계처리방법을 병행하였다. 태토의 주요산화물에 대한 비교분석결과 유천리 청자는 Seger 식과 주성분분석에서 RO_(2)와 RO+R_(2)O가 6~8 및 0.3~0.38 mole로, 또 높은 SiO_(2)함량과 낮은 Na_(2)O, CaO의 함량으로 전북지역의 데이터 범위에 분포함을 볼 수 있었다. 유약의 주요산화물에 대한 비교분석결과에서는 Seger식과 주성분분석에서 모두 명확한 지역구분이 나타나지 않았으며, 따라서 유천리 청자 역시 명확한 지역적 특성을 구분하기 어려웠다. 태토중 미량성분의 주성분분석에 의한 통계처리결과 광주 충효동 도편들을 제외하고는 뚜렷한 지역간의 구분을 관찰하기 어려웠다. The characterization of chemical properties and analogy of manufactured origin for the eleven celadon sherds collected in Yuchunri, Buan were performed by analyzing their body and glaze compositions and compared with previous data reported. The chemical compositions of the body and glaze were determined by XRF and EPMA, respectively. The trace elements of the body were also determined by ICP-MS. The Seger analysis and PCA were used to compare the major compositions of body and glaze of the potteries we found with previous data. The Celadon sherds from Yuchunri are distributed in a range of Chonnam potteries considering RO_(2) and RO+R_(2)O are 6~ 8 and 0.3 ~O.38mole, respectively and also high in SiO_(2) and low in Na_(2)O and CaO concentrations in the comparison between the potteries for the major compositions of the body. Differences of the chemical characteristics of those potteries between the provinces were not clear in comparison of the glaze compositions and trace element of the body.

血小板凝集에 미치는 Adenosine Diphosphate Ribose의 影響

李康萬 全南大醫大 1970 전남의대학술지 Vol.7 No.4

Hematin의 溶血作用

李康萬 全南大醫大 1970 전남의대학술지 Vol.7 No.4