Numerical Simulation of Thermal Fluctuation of Hot and Cold Fluids Mixing in a Tee Junction

Kai Gao,Tao Lu 국제문화기술진흥원 2015 International Journal of Advanced Culture Technolo Vol.3 No.2

In this work, mixing processes of hot and cold fluids of three different jet types are predicted by large-eddy simulation (LES) on FLUENT platform. Temperature at different positions of internal wall and mixing conditions of T-junctions at different times are obtained, then the simulated normalized mean and root-mean square (RMS) temperature, temperature contour and velocity vector of every case are compared. The results indicate that, the mixing regions in the tee junction is related to the jet type, and temperature fluctuations on the pipe wall in the type of the deflecting jet is the least.

Comparison of rectum fecal bacterial community of finishing bulls fed high-concentrate diets with active dry yeast and yeast culture supplementation

Gao Kai,Geng Chunyin 아세아·태평양축산학회 2023 Animal Bioscience Vol.36 No.1

Objective: The objective of this study was to investigate the effects of feeding active dry yeast (ADY) and yeast culture (YC) on fecal bacterial community in finishing bulls fed highconcentrate diets in the same experimental environment. Methods: Forty-five healthy finishing cattle (Simmental×Chinese Luxi yellow bulls; 24 months; 505±29 kg) were randomly divided into three groups: i) CON group (control group, only fed basal diet), ii) ADY group (fed basal diet + active dry yeast), and iii) YC group (fed basal diet + yeast culture). At the end of the trial, nine rectum fecal samples were randomly selected from each group for bacterial DNA sequencing. Results: There was no difference among groups about alpha diversity indices (all p>0.05), including ACE, Chao 1, Shannon, and Simpson indices. Principal component analysis and non-metric multidimensional scaling analysis showed a high similarity among three groups. Compared with CON group, ADY and YC groups had greater relative abundance of c_Clostridia, o_Oscillospirales, and f_Oscillospiraceae, but lesser relative abundance of g_Megasphaera, and s_Megasphaera_elsdenii (all p<0.01). And, the relative abundances of p_Firmicutes (p = 0.03), s_Prevotella_sp (p = 0.03), o_Clostridiales (p<0.01), g_Clostridium (p<0.01), f_Caloramatoraceae (p<0.01), and f_Ruminococcaceae (p = 0.04) were increased in the ADY group. The PICRUSt2 prediction results showed that the metabolic pathways had no significant differences among groups (p>0.05). Besides, the relative abundance of c_Clostridia (r = 0.42), and f_Oscillospiraceae (r = 0.40) were positively correlated to average daily gain of finishing bulls (p<0.05). Conclusion: Both of ADY and YC had no effect on diversity of fecal bacteria in finishing bulls, but the supplementation of ADY and YC can improve the large intestinal function in finishing bulls by increasing the abundance of cellulolytic bacteria and altering the abundance of lactic acid-utilizing bacteria.

Numerical Simulation of Thermal Fluctuation of Hot and Cold Fluids Mixing in a Tee Junction

Gao, Kai,Lu, Tao The International Promotion Agency of Culture Tech 2015 International Journal of Advanced Culture Technolo Vol.3 No.2

In this work, mixing processes of hot and cold fluids of three different jet types are predicted by large-eddy simulation (LES) on FLUENT platform. Temperature at different positions of internal wall and mixing conditions of T-junctions at different times are obtained, then the simulated normalized mean and root-mean square (RMS) temperature, temperature contour and velocity vector of every case are compared. The results indicate that, the mixing regions in the tee junction is related to the jet type, and temperature fluctuations on the pipe wall in the type of the deflecting jet is the least.

Dynamic Beehive Detection and Tracking System Based on YOLO V5 and Unmanned Aerial Vehicle

Gao Peng,이강빈,쿠스위디얀토 루카스 위쿠,유승화,Hu Kai,Liang Gaotian,Chen Yufeng,Wang Weixing,Liao Fei,정유석,전문석,최인찬,한웅철 한국농업기계학회 2022 바이오시스템공학 Vol.47 No.4

Purpose With urban development and improvements in human living conditions, wild beehives in densely populated areas present a threat to human safety. The traditional manual method of clear beehives may result in secondary injury to humans. Methods This paper proposes a beehive detection model based on YOLO V5 by introducing the Shufe Block V2 and depthwise separable convolution (DSC) modules to decrease the original model parameters. The model can be deployed on edge computation devices such as Raspberry 4B with good detection accuracy. The PID algorithm and dual servo motors were combined with the object detection model to track the beehive automatically. The results of experiments showed that the inference speed of the improved beehive detection model was 92.5% faster than the original YOLO V5s model, although the detection accuracy and other indicators were not signifcantly diferent. Results The accuracy of the system in this study was as high as 96% in real-time detection, and the maximum recognition distance was 2.5 m. The performance test results of the system deployed on an unmanned aerial vehicle (UAV) showed that 90% of the beehive tracking process could be completed within 2 s, positioning the object in the center of the images collected by the camera. At the same time, when the UAV was moving at random, the detection and tracking system could still follow the beehive quickly and automatically. Conclusion The detection model and tracking system established in this paper provide important support to reduce the secondary damage to the rescue workers that may occur in beehive governance.

Relationship of Phenolic Compounds and Free-radical Scavenging Activity in Black and Red Rice Extract

Gao Sheng Hu,Kai Xiu Xu,Soon Jae Jeong,Doh Hoon Kim 한국육종학회 2010 한국육종학회지 Vol.42 No.2

Total phenolic content, total anthocyanin content and antioxidant activity were analyzed from rice samples collected in Korea, Japan and China. The results showed that the total phenolic content and free-radical scavenging activity differed significantly in these rice lines. The correlation between content and activity was subsequently investigated. The results showed that in black rice, anthocyanin was the major phenolic component and that both phenolic content and anthocyanin content were closely correlated with free-radical scavenging activity. Reverse Phase High Performance Liquid Chromatography (RP-HPLC) data showed that cyanidin-3-O-glucoside and peonidin-3-O-glucoside composed about 90% of the total anthocyanin content in black rice and in red rice. In the red rice extract, the total phenolic content produced a high correlation coefficient with antioxidant activity but correlated very poorly with the total anthocyanin content. The OD458 and the OD500 values which represent the proanthocyanidin content of the rice extract, produced high correlation coefficients with antioxidant activity and total phenolic content. These results suggest that the OD458 and the OD500 values can be used to evaluate the quality of red rice. In addition, based on the data obtained, a competitive accumulation model of anthocyanin and proanthocyanidin in black and red rice was proposed.

Long-term structural analysis and stability assessment of three-pinned CFST arches accounting for geometric nonlinearity

Kai Luo,Yong-Lin Pi,Wei Gao,Mark A.Bradford 국제구조공학회 2016 Steel and Composite Structures, An International J Vol.20 No.2

Due to creep and shrinkage of the concrete core, concrete-filled steel tubular (CFST) arches continue to deform in the long-term under sustained loads. This paper presents analytical investigations of the effects of geometric nonlinearity on the long-term in-plane structural performance and stability of three-pinned CFST circular arches under a sustained uniform radial load. Non-linear long-term analysis is conducted and compared with its linear counterpart. It is found that the linear analysis predicts long-term increases of deformations of the CFST arches, but does not predict any long-term changes of the internal actions. However, non-linear analysis predicts not only more significant long-term increases of deformations, but also significant long-term increases of internal actions under the same sustained load. As a result, a three-pinned CFST arch satisfying the serviceability limit state predicted by the linear analysis may violate the serviceability requirement when its geometric nonlinearity is considered. It is also shown that the geometric nonlinearity greatly reduces the long-term in-plane stability of three-pinned CFST arches under the sustained load. A three-pinned CFST arch satisfying the stability limit state predicted by linear analysis in the long-term may lose its stability because of its geometric nonlinearity. Hence, non-linear analysis is needed for correctly predicting the long-term structural behaviour and stability of three-pinned CFST arches under the sustained load. The non-linear long-term behaviour and stability of three-pinned CFST arches are compared with those of twopinned counterparts. The linear and non-linear analyses for the long-term behaviour and stability are validated by the finite element method.

Trichoderma biodiversity in major ecological systems of China

Kai Dou,Jinxin Gao,Chulong Zhang,Hetong Yang,Xiliang Jiang,Jishun Li,Yaqian Li,Wei Wang,Hongquan Xian,Shigui Li,Yan Liu,Jindong Hu,Jie Chen 한국미생물학회 2019 The journal of microbiology Vol.57 No.8

An investigation of Trichoderma biodiversity involving a large-scale environmental gradient was conducted to understand the Trichoderma distribution in China. A total of 3,999 isolates were isolated from forestry, grassland, wetland and agriculture ecosystems, and 50 species were identified based on morphological characteristics and sequence analysis of genetic markers. Trichoderma harzianum showed the largest proportion of isolates and the most extensive distribution. Hypocrea semiorbis, T. epimyces, T. konilangbra, T. piluliferum, T. pleurotum, T. pubescens, T. strictipilis, T. hunua, T. oblongisporum and an unidentified species, Trichoderma sp. MA 3642, were first reported in China. Most Trichoderma species were distributed in Jilin and Heilongjiang Provinces in northeast China and the fewest were distributed in Qinghai Province. Based on the division of ecological and geographic factors, forestry ecosystems and low-altitude regions have the greatest species biodiversity of Trichoderma.

CK2 phosphorylates AP-2α and increases its transcriptional activity

( Kai Qun Ren ),( Shuang Lin Xiang ),( Fang Li He ),( Wen Feng Zhang ),( Xiao Feng Ding ),( Yan Yang Wu ),( Li Ping Yang ),( Jian Lin Zhou ),( Xiang Gao ),( Jian Zhang ) 생화학분자생물학회(구 한국생화학분자생물학회) 2011 BMB Reports Vol.44 No.7

Transcription factor AP-2α involves in the process of mammalian embryonic development and tumorigenesis. Many studies have shown that AP-2α functions in association with other interacting proteins. In a two-hybrid screening, the regulatory subunit β of protein casein kinase 2 (CK2β) was identified as an interacting protein of AP-2α; we confirmed this interaction using in-vitro GST pull-down and in-vivo co-immunoprecipitation assays; in an endogenous co-immunoprecipitation experiment, we further found the catalytic subunit α of protein casein kinase 2 (CK2α) also exists in the complex. Phosphorylation analysis revealed that AP-2α was phosphorylated by CK2 kinase majorly at the site of Ser429, and such phosphorylation could be blocked by CK2 specific inhibitor 4,5,6,7-tetrabromobenzotriazole (TBB) in a dose-dependent manner. Luciferase assays demonstrated that both CK2α and CK2β enhanced the transcription activity of AP-2α; moreover, CK2β increased the stability of AP-2α. Our data suggest a novel cellular function of CK-2 as a transcriptional co-activator of AP-2α.

Adalimumab induction and maintenance therapy achieve clinical remission and response in Chinese patients with Crohn`s disease

( Kai Chun Wu ),( Zhi Hua Ran ),( Xiang Gao ),( Minhu Chen ),( Jie Zhong ),( Jian Qiu Sheng ),( Michael A Kamm ),( Simon Travis ),( Kori Wallace ),( Nael M Mostafa ),( Marisa Shapiro ),( Yao Li ),( Ro 대한장연구학회 2016 Intestinal Research Vol.14 No.2

Background/Aims: This was a Phase 2 study (NCT02015793) to evaluate the pharmacokinetics, safety, and efficacy of adalimumab in Chinese patients with Crohn`s disease (CD). Methods: Thirty, adult Chinese patients with CD (CD Activity Index [CDAI] 220-450; high-sensitivity [hs]-C-reactive protein [CRP] ≥3 mg/L) received double-blind adalimumab 160/80 mg or 80/40 mg at weeks 0/2, followed by 40 mg at weeks 4 and 6. An open-label extension period occurred from weeks 8-26; patients received 40 mg adalimumab every other week. Serum adalimumab concentration and change from baseline in fecal calprotectin (FC) were measured during the double-blind period. Clinical remission (CDAI <150), response (decrease in CDAI ≥70 points from baseline), and change from baseline in hs-CRP were assessed through week 26. Nonresponder imputation was used for missing categorical data and last observation carried forward for missing hs-CRP/FC values. No formal hypothesis was tested. Adverse events were monitored. Results: Mean adalimumab serum concentrations during the induction phase were 13.9-18.1 μg/mL (160/80 mg group) and 7.5-9.5 μg/mL (80/40 mg group). During the double-blind period, higher remission/ response rates and greater reductions from baseline in hs-CRP and FC were observed with adalimumab 160/80 mg compared to that with 80/40 mg. Adverse event rates were similar among all treatment groups. Conclusions: Adalimumab serum concentrations in Chinese patients with CD were comparable to those observed previously in Western and Japanese patients. Clinically meaningful remission rates and improvement in inflammatory markers were achieved with both dosing regimens; changes occurred rapidly with adalimumab 160/80 mg induction therapy. No new safety signals were reported. (Intest Res 2016;14:152-163)

STUDY OF INTELLIGENT ENGINEERING DRAWING PROCESSING SYSTEM

Gao Guoan,Gong Mao Kai,Zhang Wei,Shen Yun Feng 대한전자공학회 1992 대한전자공학회 Conference Vol.1992 No.2