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Yuanzheng Wu,Hetong Yang,전영진,이민영,Jishun Li,신현재 한국생물공학회 2014 Biotechnology and Bioprocess Engineering Vol.19 No.4
A copper(I)-catalyzed azide-alkyne cycloaddition(CuAAC) reaction was exploited for the surface modificationof cowpea chlorotic mottle virus (CCMV). The exposedcarboxyl residues of the CCMV capsids were modifiedwith an alkyne and then further modified with an azide,using a triazole connection in the presence of CuSO4,tris(2-carboxyethyl)phosphine hydrochloride (TCEP), anda bathocuproin disulfonic acid disodium salt (BCDS). Fluorogenic coumarin was successfully grafted onto theCCMV capsids and monitored by fast protein liquidchromatography (FPLC) and UV-irradiated SDS-PAGE. An oligo-ethylene glycol (OEG) short chain and an Arg-Gly-Asp (RGD) peptide were also connected to the CCMVcapsids via the CuAAC reaction. Size-exclusion FPLC,transmission electron microscopy (TEM), and dynamiclight scattering (DLS) analyses confirmed the modificationand integrity of the viral capsids. Interestingly, OEG-CCMVdisplayed a unique phenomenon of connected bridges withthe intact capsids crosslinked to each other. Coumarin-CCMV, OEG-CCMV, and RGD-CCMV were absorbedonto APTES slides for cell binding with HeLa cells. Theopposite adhesion behavior of OEG-CCMV and RGDCCMVindicated the inhibition effect of OEG and thepromotion effect of RGD for cell attachment. This providesa generalized method for chemical modification of thesurface of virus capsids with multivalent ligands, whichdemonstrates the potential applications in bioimaging, tissueengineering, and drug delivery.
Fungal and mushroom hydrophobins: A review
Yuanzheng Wu,Jishun Li,Hetong Yang,Hyun-Jae Shin 한국버섯학회 2017 한국버섯학회지 Vol.15 No.1
Hydrophobins are surface active proteins that are produced by filamentous fungi including mushrooms. Their ability to self-assemble into an amphipathic membrane at any hydrophilic–hydrophobic interface is most intriguing. These small secreted proteins comprise of eight conserved cysteine residues which form four disulfide bridges and an extraordinary hydrophobic patch. Hydrophobins play critical roles in fungal (and/or mushrooms) growth as structural components and in the interaction of fungi and mushrooms with the environment. The biophysical and biochemical properties of the isolated proteins are remarkable, such as strong adhesion, high surface activity and the formation of various self-assembled structures. With the increasing demands of hydrophobins from fungi and mushroom sources, production and purification in large scale is under challenge. Various applications, ranging from food industries, cosmetics, nanotechnology, biosensors and electrodes, to biomaterials and pharmaceuticals are emerging and a bright future is foreseen.
Yuanzheng Wu,Jishun Li,Hetong Yang,성지현,임호동,김근중,신현재 한국생물공학회 2017 Biotechnology and Bioprocess Engineering Vol.22 No.6
Our aim was to devise targeted drug delivery systems using genetically modified cowpea chlorotic mottle virus (CCMV) capsids by fusion expression with tumorhoming peptide F3 for efficient delivery of therapeutic substances into tumor cells. The RNA-binding domain at the N terminus (amino acid residues 1–25) of CCMV capsid protein (CP) was selectively deleted, and F3 was inserted for the expression in Pichia pastoris. After chromatographic purification, F3-CCMV capsids were obtained via selfassembly of the F3-CP fusion protein and then analyzed by transmission electron microscopy and dynamic light scattering analysis, which revealed spherical nanoparticles (NPs) ca. 18 nm in diameter with regular monodispersity. Near-infrared fluorescent dye IR780 iodide, which has been applied for cancer imaging, photodynamic therapy, and photothermal therapy, was encapsulated in F3-CCMV NPs. The resultant F3-CCMV-IR780 NPs showed excellent molecular targeting to nucleolin receptor overexpressed on the surface of MCF-7 tumor cells. Furthermore, the in vitro cellular uptake and cell viability assay proved a photothermal effect by a single dose of near-infrared laser irradiation. The present system may offer a programmable nanoscaffoldbased drug delivery system vehicle for fabrication of promising therapeutic substances for cancer therapy.
Viruses as self-assembled nanocontainers for encapsulation of functional cargoes
신현재,Yuanzheng Wu,Hetong Yang 한국화학공학회 2013 Korean Journal of Chemical Engineering Vol.30 No.7
Viruses naturally exhibit an incredible variety of sophisticated nanostructures, which makes them ideal biological building blocks for nanoengineered material research. By mimicking their spontaneous assembly process,tremendous advances have been made towards utilizing virus and virus-like particles (VLPs) as protein cages, scaffolds,and templates for nanomaterials in the last few years. This review outlines recent progress in the field of bionanotechnology in which viruses are introduced to encapsulate various functional cargoes in a precise and controlled fashion. The encapsulation mechanisms are summarized into three main strategies: electrostatic interaction, chemical conjugation,and covalent attachment by genetic manipulation. The combination with chemical modification and genetic engineering heralds a brilliant future for fabrication of functional nanomaterials. These well-defined architectures will find attractive applications in biosensing, drug delivery, enzyme confinement, light-harvesting system, and pharmaceutical therapy.
Fungal and mushroom hydrophobins: A review
Wu, Yuanzheng,Li, Jishun,Yang, Hetong,Shin, Hyun-Jae The Korean Society of Mushroom Science 2017 한국버섯학회지 Vol.15 No.1
Hydrophobins are surface active proteins that are produced by filamentous fungi including mushrooms. Their ability to self-assemble into an amphipathic membrane at any hydrophilic-hydrophobic interface is most intriguing. These small secreted proteins comprise of eight conserved cysteine residues which form four disulfide bridges and an extraordinary hydrophobic patch. Hydrophobins play critical roles in fungal (and/or mushrooms) growth as structural components and in the interaction of fungi and mushrooms with the environment. The biophysical and biochemical properties of the isolated proteins are remarkable, such as strong adhesion, high surface activity and the formation of various self-assembled structures. With the increasing demands of hydrophobins from fungi and mushroom sources, production and purification in large scale is under challenge. Various applications, ranging from food industries, cosmetics, nanotechnology, biosensors and electrodes, to biomaterials and pharmaceuticals are emerging and a bright future is foreseen.
Trichoderma biodiversity in major ecological systems of China
Kai Dou,Jinxin Gao,Chulong Zhang,Hetong Yang,Xiliang Jiang,Jishun Li,Yaqian Li,Wei Wang,Hongquan Xian,Shigui Li,Yan Liu,Jindong Hu,Jie Chen 한국미생물학회 2019 The journal of microbiology Vol.57 No.8
An investigation of Trichoderma biodiversity involving a large-scale environmental gradient was conducted to understand the Trichoderma distribution in China. A total of 3,999 isolates were isolated from forestry, grassland, wetland and agriculture ecosystems, and 50 species were identified based on morphological characteristics and sequence analysis of genetic markers. Trichoderma harzianum showed the largest proportion of isolates and the most extensive distribution. Hypocrea semiorbis, T. epimyces, T. konilangbra, T. piluliferum, T. pleurotum, T. pubescens, T. strictipilis, T. hunua, T. oblongisporum and an unidentified species, Trichoderma sp. MA 3642, were first reported in China. Most Trichoderma species were distributed in Jilin and Heilongjiang Provinces in northeast China and the fewest were distributed in Qinghai Province. Based on the division of ecological and geographic factors, forestry ecosystems and low-altitude regions have the greatest species biodiversity of Trichoderma.
Yuanzheng Wu(오원정),Inhwa Han(한인화),Byung-Geon Park(박병건),Jishun Li(이지순),Hetong Yang(양해통),Hyun-Jae Shin(신현재) 한국생물공학회 2016 KSBB Journal Vol.31 No.3
The effects of medicinal mushroom (Sparassis crispa) powder supplementation on the rheological property of dough and the quality of bread were investigated. Naturally dried S. crispa powders (NDSCP) and freeze-dried ones (FDSCP) were prepared. Farinograph profiles of dough showed that mushroom powder addition at levels higher than 1% led to reduced dough stability suggested by decreased development time and increased weakness. The incorporation of S. crispa powders was significantly (p<0.05) lower compared to other mushrooms which might be attributed to its high content of beta-glucan. FDSCP was chosen over NDSCP because of its fragrant flavor which could be ascribed to the preservation of volatile polyphenol components. The quality of FDSCP bread was evaluated with respect to specific volume, texture, color, and organoleptic qualities. The specific volume of bread with S. crispa powder decreased compared to the control, while textural properties, such as hardness, chewiness, and gumminess, were enhanced. Sensory evaluation showed that 0.3% FDSCP incorporation presented the highest bread quality. With its daily consumption, S. crispa supplemented bread can provide consumers with multiple health benefits.