Paricalcitol attenuates lipopolysaccharide-induced inflammation and apoptosis in proximal tubular cells through the prostaglandin E₂ receptor EP4

( Yu Ah Hong ),( Keum Jin Yang ),( So Young Jung ),( Yoon Kyung Chang ),( Cheol Whee Park ),( Chul Woo Yang ),( Suk Young Kim ),( Hyeon Seok Hwang ) 대한신장학회 2017 Kidney Research and Clinical Practice Vol.36 No.2

Background: Vitamin D is considered to exert a protective effect on various renal diseases but its underlying molecular mechanism remains poorly understood. This study aimed to determine whether paricalcitol attenuates inflammation and apoptosis during lipopolysaccharide (LPS)-induced renal proximal tubular cell injury through the prostaglandin E₂ (PGE₂) receptor EP4. Methods: Human renal tubular epithelial (HK-2) cells were pretreated with paricalcitol (2 ng/mL) for 1 hour and exposed to LPS (1 μg/mL). The effects of paricalcitol pretreatment in relation to an EP4 blockade using AH-23848 or EP4 small interfering RNA (siRNA) were investigated. Results: The expression of cyclooxygenase-2, PGE₂, and EP4 were significantly increased in LPS-exposed HK-2 cells treated with paricalcitol compared with cells exposed to LPS only. Paricalcitol prevented cell death induced by LPS exposure, and the cotreatment of AH-23848 or EP4 siRNA offset these cell-protective effects. The phosphorylation and nuclear translocation of p65 nuclear factor-kappaB (NF-κB) were decreased and the phosphorylation of Akt was increased in LPS-exposed cells with paricalcitol treatment. AH-23848 or EP4 siRNA inhibited the suppressive effects of paricalcitol on p65 NF-κB nuclear translocation and the activation of Akt. The production of proinflammatory cytokines and the number of terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling-positive cells were attenuated by paricalcitol in LPS exposed HK-2 cells. The cotreatment with an EP4 antagonist abolished these anti-inflammatory and antiapoptotic effects. Conclusion: EP4 plays a pivotal role in anti-inflammatory and antiapoptotic effects through Akt and NF-κB signaling after paricalcitol pretreatment in LPS-induced renal proximal tubule cell injury.

한국인 직무 스트레스 측정도구의 개발 및 표준화

장세진,고상백,강동묵,김성아,강명근,이철갑,정진주,조정진,손미아,채창호,김정원,김정일,김형수,노상철,박재범,우종민,김수영,김정연,하미나,박정선,이경용,김형렬,공정옥,김인아,김정수,박준호,현숙정,손동국 大韓産業醫學會 2005 대한직업환경의학회지 Vol.17 No.4

Background and Purposes: Over the past three decades, numerous studies performed in Korea have reported that job stress is a determinant risk factor for chronic diseases and work disability. Every society has its own culture and occupational climate particular to their organizations, and hence experiences different occupational stress. An occupational stress measurement tool therefore needs to be developed to estimate it objectively. The purpose of this study is to develop and standardize the Korean Occupational Stress Scale (KOSS) which is considered to be unique and specific occupational stressors in Korean employees. Subjects and Methods: Data were obtained from the National Study for Development and Standardization of Occupational Stress (NSDSOS Project: 2002-2004). A total of 12,631 employees from a nationwide sample proportional to the Korean Standard Industrial Classification and the Korean Standard Occupational Classification were administered. The KOSS was developed for 2 years (2002-2004). In the first year, we collected 255 items from the most popular job stress measurement tools such as JCQ, ERI, NIOSH and OSI, and 44 items derived from the a qualitative study (depth interview). Forty-three items of KOSS, in the second year, were retained for use in the final version of the KOSS by using Delphi and factor analysis. Items were scored using conventional 1-2-3-4 Likert scores for the response categories. Results: We developed eight subscales by using factor analysis and validation process: physical environment (3 items), job demand (8 items), insufficient job control (5 items), interpersonal conflict (4 items), job insecurity (6 items), organizational system (7 items), lack of reward (6 items), and occupational climate (4 items). Together they explained 50.0% of total variance. Internal consistency alpha scores were ranged from 0.51 to 0.82. Twenty-four items of the short form of the KOSS (KOSS-SF) were also developed to estimate job stress in the work setting. Because the levels of the subscales of occupational stress were gender dependent, gender-specific standard norms for both the 43-item full version and the 24-item short form using a quartile for the subscales of KOSS were presented. Conclusion: The results of this study suggest that KOSS might be an appropriate measurement scale to estimate occupational stress of Korean employees. Further and more detailed study needs to be conducted to improve the validity of this scale.

곡류 중의 섬유질이 흰쥐의 무기질 흡수율에 미치는 영향

정경아,장유경 漢陽大學校 韓國生活科學硏究所 1996 韓國 生活 科學 硏究 Vol.- No.14

This study was performed to investigate the effect of cereal fibers on mineral apparent absorption in rats. Male Sprague-Dawley rats weighing 180±4g were divided into five groups by completely randomized block design : R, BR, B, W and F. Each group fed a diet containing a kind of cereal among rice (R), brown rice (BR), barley (B), whole wheat (W) and wheat flour (F) for 5 weeks. The results were summarized as follows : 1) Food intake, weight gain and feed efficiency ratio were not significantly different among the groups 2) Fecal Ca was significantly the most in the W group compared to the others. And Ca apparent absorption was significantly lower in the W, B and BR group than in the other two groups. 3) Fecal P was significantly more in the W, B and BR groups than in the other two groups. And P apparent absorption was significantly lower in the W, B, and BR groups than in the other two groups. 4) Fecal Mg was significantly more in the W, B, BR groups than in the other two groups. And Mg apparent absorption was significantly lower in the W, B, BR groups than in the other two groups. 5) Fecal Zn was significantly the most in the W group compared to the others. And Zn apparent absorption was significantly the lowest in the W group compared to the others. 6) Comparing absorption of minerals at the first and last week, absorption of minerals except Zn was increased at the last week. From the results of this study, we can decide that cereals containing much insoluble dietary fiber can interrupt the apparent absorption of minerals

Helicobacter pyloir 양성 소화성 궤양에서 lansoprazole 포함 삼제요법의 제균율 및 제균판정에 있어서 요소호기검사의 유용성

정혜경,곽재진,유민아,배기선,권정미,이종수,김도영,문일환 대한감염학회 2003 감염과 화학요법 Vol.35 No.3

목적 : 본 연구는 Helicobacter pylori (이하 H. polyri) 양성인 소화성 궤양 환자에서 lansoprazole 포함 삼제요법의 제균율을 알아보고, 제균 치료 후 제균 판정에 있어서 요소 호기 검사(Urea breath test, 이하 UBT) 및 신속요소분해(rapid urease test, 이하 RUT test)와의 일치율에 대하여 알아보고자 하였다. 방법 : 상부위장관내시경 검사에서 H. polyri 양성인 소화성 궤양환자에서 lansoprazole 60㎎+amoxicillin 2g+clarithromycin 1g의 약제를 2회 분복하여 1주간 복용하는 삼제요법을 실시하였다. 최소 4주후 추적 상부위장관내시경을 실시하였고, 전정부와 체부에서 각각 RUT를 실시하였으며 UBT(5분, 20분)를 시행하였다. 결과 : 대상환자는 총 46명으로 남자 34명(48±13세), 여자 12명(53±14세)이었다. LAC 삼제요법의 제균율은 40/46명(87.0%)이었고, 궤양의 치유율도 42/46명(91.3%)이었다. RUT와 20분 UBT 결과가 모두 음성이었던 경우는 41예, 모두 양성이었던 경우는 4예로 RUT와 UBT는 97.8% (45/46)의 일치율을 보였고, 1예는 UBT는 음성이었으나 체부에서 시행한 RUT가 양성이었다. 5분 UBT 검사가 양성이었던 예는 14/34 (41.2%) 이었고, 이 14예 중 12예(85.7%)는 20분 UBT 검사 음성, RUT 음성이었다. 결론 : Lansoprazole과 amoxicillin, clarithromycin 1주일 병합요법은 H. polyri 양성인 소화성 궤양 환자에서 87.0%의 제균율을 나타내어 우수한 효과를 보였고, 요소호기검사는 제균 치료 후 제균 판정에 유용한 비침습적인 방법임을 확인할 수 있었다. Background : There are only a few studies on Helicobacter pylori (H. pylori) for its eradication rates of lansoprazole-based triple therapy in Korea, and the results are controversial. Therefore, we undertook to investigate the eradication rate of lansoprazole-based triple therapy, and compare the concordance rate of urea breath test (UBT) and rapid urease test (RUT) in evaluating H. pylori eradication. Methods : Patients with acute peptic ulcer who were H. pylori-positive were recruited by prospective, consecutive manner. They received lansoprazole 30 ㎎ b.d., amoxicillin 1 g b.d. and clarithromycin 500 ㎎ b.d. for 1 week. Upper endoscopy was performed after 4 weeks to check for ulcer healing, and UBT and RUT were performed to evaluate H. pylori eradication status. Results : A total of 46 patients were recruited, and they were all compliant. H. pylori eradication rate was 87.0% (40/46) and ulcer healing rate was 91.3% (42/46). Forty one patients showed negative in both UBT and RUT, and 4 patients revealed positive in both tests, therefore, the concordance rate of UBT and RUT was 97.8% (45/46). Conclusion : Our study showed that 1-week lansoprazole-based triple therapy was effective in H. pylori eradication and ulcer healing. UBT can be an effective, noninvasive method for evaluating H. pylori status after H. pylori eradication.

폐경 후 여성의 심혈관질환 예방을 위한 식사중재에 대한 준수도(adherence) 평가

정경아,김상연,우정익,장유경 漢陽大學校 韓國生活科學硏究所 2001 韓國 生活 科學 硏究 Vol.- No.19

The purpose of this study is to assess the adherence to dietary intervention for prevention of coronary heart disease (CHD) in postmenopausal women by observing changes of antioxidant vitamins and fatty acid composition in dietary intake and seam level. The subjects with hypercholesterolemia (TC≥240mg/dl) were treated with one of hormone replacement therapy (HRT group), dietary intervention (DIET group) or combination of hormone replacement therapy and dietary intervention (HRT+DIET group) for 12 weeks. The results were as followed. Mean daily nutrient intakes were not significantly changed in the HRT group, but were significantly changed in the DIET group and the HRT+DIET group. Especially, dietary vitamin A and E intakes were increased from less than 75% to more than 90% of RDA for Korean in the two groups. With changes of dietary intakes, serum vitamin A and E levels were also significantly increased in the DIET group and tended to increase in the HRT+DIET group. Dietary fatty acid composition was also not significantly changed in the HRT group, but was significantly chanced in the DIET group and the HRT+DIET group. With changes of dietary fatty acid composition, serum phospholipid fatty acid composition was significantly changed in the DIET group and the HRT+DIET group although there was tendency of returning to initial value after 12 weeks. Judging from changes in serum phospholipid fatty acid composition, subjects' adherence to dietary intervention tended to decrease after 12 weeks. However, the changes of antioxidant vitamins and fatty acid composition in dietary intake were compatible with the changes of those in serum level, and we can conclude that dietary intervention was adhered quite well by subjects.

신장기증자들의 심리적 특성

정승아,김재환,강종명,이장한,김경욱 漢陽大學校 精神健康硏究 1998 精神健康硏究 Vol.17 No.-

상거래 주체간 신뢰성을 제공하는 CORBA 기반 보안 서비스

장경아,유정채,이용진 高麗大學校附設 컴퓨터科學技術硏究所 1999 JOURNAL OF COMPUTER SCIENCE & ENGINEERING TECHNOLO Vol.1 No.-

대단위 분산 환경에서 전자상거래를 활성화하기 위해 필수적으로 참여자 인증 서비스가 제공되어야 한다. 본 연구에서는 OMG에서 제시한 CORBA 보안 명세를 기반으로 개방형 분산 환경에 대한 보안 서비스를 전자상거래에 적용하여 원격 상거래 주체 사이의 상호 인증 구조로 제안한다. 상거래 참여자들에 대한 객체 단위 주체 인증 기법과 인증 키 교환 기법으로 공급자와 수요자, 관련 응용 서비스들 사이의 상호 신뢰성을 제공하도록 한다. 이 기법으로 상대 거래 주체의 신원 확인 뿐 아니라 거래 진행 중 취득한 정보의 근원을 파악할 수 있도록 하였으며 OMG에서 제시한 도메인 접근 정책을 통합하여 보안 수준을 향상시킬 수 있었다. 또한 CORBA 미들웨어에 기반하여 수행되므로 기술적 중립성을 바탕으로 대규모 개방형 분산 시스템 확장에 유리할 것이다. Most of all there must be provided entity authentication for activation of electronic commerce in large scale distributed computing system. In this paper, we propose the mutual authentication scheme for Electronic Commerce entity based on CORBA security service specification that OMG defined. And this scheme is provided authentication of object-oriented commerce entity and authenticated key exchange. These techniques provide the identity of a commerce partner entity, but also have confidence that data associated to commerce flows originates with this partner. Additionally, as the used CORBA Security Service Specification provides domain access control policy, it is more efficiently applicable to a mutual authentication. Therefore, as the CORBA application has provided the technology of neutral, it is useful for the distributed system to scalable.

Scanning Probe Microscopy를 이용한 자기조립 구조 Bio-molecule 및 DNA의 특성 분석

兪炅我,姜致中,金容商 明知大學校 産業技術硏究所 2005 産業技術硏究所論文集 Vol.24 No.-

In this paper, we characterized of assembly-structure of bio-molecule on the gold surface by Scanning probe microscopy(SPM), aiming to apply to the biosensors and bioelectronic systems. For the formation of the molecular structures, gold substrate was deposited on the silicon substrate first, and the cystamine terminated with thiol and amine group. Thiol group of cystamine was covalently immobilized on the gold surface. By characterizing this self assembled interaction with SPM. And Bio-molecules such as DNA, protein might interact with substrate where they are deposited, resulting in the change of conformation and physical, electrical properties. So, it is important to analyze properly the local characteristics of those molecules w.r.t. the ambient environment. By using scanning probe microscopy, we could manipulate DNA molecules electrically and measure their properties.

백혈병 세포에서 Multidrug Resistance Gene-1 (mdr1)의 과발현이 ^99m Tc-sestaMIBI 섭취에 미치는 영향

천경아,이재태,이상우,강도영,손상균,이종기,정준기,전수한,이규보 경북대학교 의학연구소 2000 경북대학교병원의학연구소논문집 Vol.4 No.1

Purpose: To determine whether 99mTc-MIBI is recognized by the multidrug resistant P-glycoprotein (Pgp), we have measured quantitatively 99mTc-MIBI uptake in cancer cells. The effects of various Pgp reversing agents on cellular 99mTc-MIBI uptake were also investigated in the presence of multidrug resistance gene-1(mdr1 gene) overexpression. Materisls and Methods: We measured percentage uptake of 99mTc-MIBI at different incubation temperatures both in mdr1 positive and negative cells. The effects of verapamil, cyclosporin, and dipyridamole on cellular uptake of 99mTc-MIBI were also evaluated with or withouts overexpression of mdr1 gene in Cultured murine leukemia L1210 cells. Results: The mdr1 gene expressing cell lines were effectively induced in in vitro with continuous application of low-dose adriamycin or vincristine. Cellular uptake of 99mTc-MIBI was higher in mdr1 negative L 1210 cells than those of mdr1 positive cells, and higher when incubated in 37℃ than 4℃. In the presence of verapamil, cyclosporin or dipyridamole, 99mTc-MIBI uptake was increased upto 604% in mdr1 positive cells. Conclusion: Cellular uptake of 99mTc-MIBI is lower in leukemia cells over-expressing mdr1 gene, and MDR-reversing agents increase cellular uptake. These results suggest that 99mTc-MIBI can be used for characterizing Pgp expression and developing MDR-reversing agents in vitro. (Korean J Nucl Med 1999;33: 152-62)

팔굽관절에서 측정방법에 따른 고유수용성감각 차이

이정아,김덕화,신화경,최규환,권오윤 한국전문물리치료학회 2003 한국전문물리치료학회지 Vol.10 No.3