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박치영,모성환,문철호,곽재정,김태종,전용준,박유환,정춘해 朝鮮大學校 附設 醫學硏究所 1995 The Medical Journal of Chosun University Vol.20 No.2
von Willebrand's disease (vWD) is the most common autosomal-dominant inherited disorder resulting from a quantitative or a qualitative defect of von Willebrand factor (vWF). The most diagnostic pattern is the combination of a prolonged bleeding time, a reduction in plasma vWF concentration, a parallel reduction in ristocetin cofactor activity, and reduced factor Ⅷ activity, In this case, ristocetin-induced platelet aggregation data were compatible with that of vWD. Bleeding times were prolonged over 4 minutes, vWF antigen levels were 45%. vWF ristocetin cofactor activities were 0~1% and factor Ⅷ levels were 31%, when compared to the normal control. We report the case of a family with vWD. characterized by a quantitative defect in vWF
기니이픽 장관의 c-Kit 및 NK 1R 면역반응 세포구조에 대한 공초점 주사현미경적 연구
장인엽,김종중,문정석,김현곤,박찬국,전제열,전규배,조철희,유호진 조선대학교 2001 The Medical Journal of Chosun University Vol.26 No.1
Background and Objectives: Immunolabelling of interstitial Cajal(IC) cells in the intestinal wall has recently been developed by using a specific marker, the anti-c-Kit antibody. Substance-P is a well-known neurotransmitter in the gastro-intestinal tract. Since the gastro-intestinal wall structures have already been well documented in the guinea pig, immunohistochemistry was done for the c-Kit-positive IC network and substance-P receptor(NK1R) in an attempt to provide a morphological basis for the mechanism regulating gastro-intestinal movement. Materials and Methods: Cryosection and whole-mount preparations of guinea pig small intestine and colon were single and double immunolabelled using the anti-c-Kit and NK1R antibodies. Immunolabelled specimens were observed under a confocal laser scanning microscope. Results : According to a three dimensional reconstruction study, it was found that (1) the c-Kit-positive celluar networks were widely distributed in the intestinal wall, (2) c-Kit-positive celluar networks encircled the ganlion, with strands in reticular configurations, and (3) the c-Kit-positive cells showed colocalization with NK1R in circular muscle(CM), not myenteric plexus(MY). Conclusion: The charateristic profiles of IC containing c-Kit-positive celluar networks and the relationship between c-Kit-positive and NK1R-positive structures provide a morphological basis upon the mechanism regulating gastro-intestinal motility.
중추에서 Prostaglandin계가 Renin-angiotensin System에 미치는 영향
최영태,김종승,문성호,오형균,김재훈,전제열,염철호,윤평진 朝鮮大學校 附設 醫學硏究所 1997 The Medical Journal of Chosun University Vol.22 No.1
Role of prostaglandins on the renin-angiotensin system in the central nervous system was examined in normotensive and 2-kidney, 1 clip (2K1C) hypertensive rats. The experiment was done under thiopental (50 ㎎/㎏, IP) anesthesia. Captopril and indomethacin were injected into the right lateral cerebral ventricle. Arterial blood pressure and heart rate were recorded from the femoral artery. Intracerebroventricular (ICV) captopril (1 ㎎) caused a decrease in mean arterial pressure in both normotensive and 2K1C hypertensive rats. The depressor response to captopril was more sensitive in hyper-tensive rats than in normotensive rats. Indomethacin treatment (ICV, 200 ㎎) altered the depressor response to captopril neither in normotensive nor in hypertensive rats. These results suggest that the cardiovascular effect of renin-angiotensin system in the central nervous system may not be mediated via prostaglandin systems in normotensive and 2KlC hypertensive rats.
師範系 體育敎育科 志望生의 一般運動能力檢査 基準値 設定에 關한 硏究
梁點紅,高基俊,黃喆文,金泰運,金重雄,白永鎬,表內淑,黃太相,徐國雄 釜山大學校 附設 體育科學硏究所 1989 體育科學硏究所 論文集 Vol.5 No.-
This study was analyzed the validity and the reliability of evaluation norm. which was used in choosing and grading events of the General Motor Ability Test. The General Motor Ability Test has been done for applicants for entrance to the department of physical education, college of education, Pusan National University. The subjects of this study were 433 applicants(men :387, women :46) for three years. They were tested in seven events : 1) 100m Dash, 2) 1500m Run(man), 800m Run (woman), 3) Throwing Hand-Ball, 4) Broad Jump, 5) Chinning(man), Flexed-Arm Hang(woman), 6) Zig-Zag Run, 7) Trunk Flexion. The results could be summerized as follows ; 1. In 100m Dash, 1500m Run, and Throwing Hand-Ball, man's average records were shown normal distribution which was inclined toward low ll grade. Therefore, the interval size of those three events must be regulated to lower. 2. Average records of Broad Jump in man were distributed into high (1) grade. So, the interval size must be adjusted to higher. 3. In Chinning, Zig-Zag Run, ad Trunk Flexion, man's average records were shawn normal distribution, and the interval size was similar to present records. 4. For woman, it was impossible to analyze correctly in all seven events because of the small number of subjects. So, it was for the sake of the reference. 5. The record of applicants for three years were operated to the variable quantity analysis. And the restults were these ; 100m Dash was F=3.0865(p<0.05), Chinning F=3.9309(p<0.05), Broad Jump F=14.7338(p<0.001), Throwing Hand-Ball F=4.9716(p<0.01), and Zig-Zag Run F=6.8297(p<0.001). Then, it was shown statistical meaningful difference in three groups. 6. In investigating the validity in choosing seven events, it was concluded that the events have been chosen reasonably. Because all events except for 100m Dash, and Zig-Zag Run in man were independent. From the analysis of the General Motor Ability Test, we could realized the necessity of regulating the standard and the interval size in several events. Then, we proposed the new evaluation norm which was suitable to present situation.
Jun-Cheol Moon,Ju-Hee Kim,Cheol Seong Jang 한국육종학회 2015 한국육종학회 심포지엄 Vol.2015 No.07
In this study, we report that the development of a multiplex PCR method using species-specific primers for the simultaneous detection of Poaceae family members, including adlay, barley, maize, rice and wheat, based on the sequence polymorphism of the DNA-directed RNA polymerase beta'' chain (rpoC2) genes Species-specific primers were constructed with common forward primer and each reverse primers which have differences on the basis of sequences. Each primer pairs could amplify PCR products of 443 bp for rice, 346 bp for barley, 278 bp for adlay, 221 bp for wheat and 96 for maize, respectively, from the five chloroplast DNAs. The series of template DNA concentrations were identified by the sensitivity of multiplex PCR. The band of products were clearly amplified from the DNA concentration range of 0.01 to 10 ng/μL. In addition, the species-specific primers were examined for the detection of seven commercial flour mixed products. The combination of the sensitivity of a multiplex PCR with the specificity of the primers for the detection of species would allow to be applied in analyses of processed foods.
Transcription factor for gene function analysis in maize
Jun-Cheol Moon,Kitae Song,Jae Yoon Kim,Seong-Bum Baek,Young-Up Kwon,Byung-Moo Lee 한국육종학회 2014 한국육종학회 심포지엄 Vol.2014 No.07
Maize is one of the most important food and feed crops in the world including Southeast Asia. In spite of numberous efforts with conventional breeding, the maize productions remain low and the loss of yields by drought and downy mildew are still severe in Asia. Genetic improvement of maize has been performed with molecular marker and genetic engineering. Because maize is one of the most widely studied crop for its own genome and has tremendous diversity and variant, maize is considered as a forefront crop in development and estimation of molecular markers for agricultural useful trait in genetics and breeding. Using QTL (Quantitative Trait Loci) and MAS (Marker Assisted Breeding), molecular breeders are able to accelerate the development of drought tolerance or downy mildew resistance maize genotype. The present paper overviews QTL/MAS approaches towards improvement of maize production against drought and downy mildew. We also discuss here the trends and importance of molecular marker and mapping population in maize breeding.