Rapid Detection and Monitoring Therapeutic Efficacy of Mycobacterium tuberculosis Complex Using a Novel Real-Time Assay

( Jiang Li Juan ),( Wen Juan Wu ),( Hai Wu ),( Son Sik Ryang ),( Jian Zhou ),( Wei Wu ),( Tao Li ),( Jian Guo ),( Hong Hai Wang ),( Shui Hua Lu ),( Yao Li ) 한국미생물 · 생명공학회 2012 Journal of microbiology and biotechnology Vol.22 No.9

We combined real-time RT-PCR and real-time PCR (R/P) assays using a hydrolysis probe to detect Mycobacterium tuberculosis complex (MTBC)-specific 16S rRNA and its rRNA gene (rDNA). The assay was applied to 28 nonrespiratory and 207 respiratory specimens from 218 patients. Total nucleic acids (including RNA and DNA) were extracted from samples, and results were considered positive if the repeat RT-PCR threshold cycle was ≤35 and the ratio of real-time RT-PCR and real-time PCR load was ≥1.51. The results were compared with those from existing methods, including smear, culture, and real-time PCR. Following resolution of the discrepant results between R/P assay and culture, the overall sensitivity, specificity, positive predictive values (PPV), and negative predictive values (NPV) of all samples (including nonrespiratory and respiratory specimens) were 98.2%, 97.2%, 91.7%, and 99.4%, respectively, for R/P assay, and 83.9%, 89.9%, 72.3%, and 94.7%, respectively, for real-time PCR. Furthermore, the R/P assay of four patient samples showed a higher ratio before treatment than after several days of treatment. We conclude that the R/P assay is a rapid and accurate method for direct detection of MTBC, which can distinguish viable and nonviable MTBC, and thus may guide patient therapy and public health decisions.

Clinical Determinants of Weight Loss in Patients with Esophageal Carcinoma During Radiotherapy: a Prospective Longitudinal View

Jiang, Nan,Zhao, Jin-Zhi,Chen, Xiao-Cen,Li, Li-Ya,Zhang, Li-Juan,Zhao, Yue Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.5

Purpose: The prevalence of weight loss in esophageal carcinoma patients is high and associated with impairment of physical function, increased psychological distress and low quality of life. It is not known which factors may contribute to weight loss in patients with esophageal carcinoma during radiotherapy in China. The objective of this study was to identify the associated demographic and clinical factors influencing weight loss. Methods: We evaluated 159 esophageal carcinoma patients between August 2010 and August 2013 in a crosssectional, descriptive study. Patient characteristics, tumor and treatment details, psychological status, adverse effects, and dietary intake were evaluated at baseline and during radiotherapy. A multivariate logistic regression analyss was performed to identify the potential factors leading to weight loss. Results: 64 (40.3%) patients had weight loss ${\geq}5%$ during radiotherapy. According to logistic regression analysis, depression, esophagitis, and loss of appetite were adverse factors linked to weight loss. Dietary counseling, early stage disease and total energy intake ${\geq}1441.3$ (kcal/d) were protective factors. Conclusions It was found that dietary counseling, TNM stage, total energy intake, depression, esophagitis, and loss of appetite were the most important factors for weight loss. The results underline the importance of maintaining energy intake and providing dietary advice in EC patients during RT. At the same time, by identifying associated factors, medical staff can provide appropriate medical care to reduce weight loss. Further studies should determine the effect of these factors on weight loss and propose a predictive model.

An Ionic Liquid Containing L-Proline Moiety as Highly Efficient and Recyclable Chiral Organocatalyst for Michael Addition

Jiang Li,Xia-Bing Li,Sha Sha Ma,Juan Liu,Ben Hao Li,Bao-Lin Li 대한화학회 2016 Bulletin of the Korean Chemical Society Vol.37 No.8

A novel chiral ionic liquid containing proline moiety was synthesized. It can be used as a highly efficient and recyclable chiral organocatalyst for Michael addition of cyclohexanone with (E)-β-nitroalkenes in methanol at room temperature. The Michael addition affords the corresponding products in satisfactory yields of isolated products (78–98%) with high diastereoselectivities and excellent enantioselectivities (up to 98% dr and up to 99% ee). The catalyst can be recycled up to five times without any decrease in yields and stereoselectivities.

Apoptosis induction by alantolactone in breast cancer MDA-MB- 231 cells through reactive oxygen species-mediated mitochondrion-dependent pathway

Li Cui,Weiquan Bu,Jie Song,Liang Feng,Tingting Xu,Dan Liu,Wenbo Ding,Jianhua Wang,Changyang Li,Binge Ma,Yi Luo,Ziyu Jiang,Chengcheng Wang,Juan Chen,Jian Hou,Hong-mei Yan,Lei Yang,Xiao-bin Jia 대한약학회 2018 Archives of Pharmacal Research Vol.41 No.3

Alantolactone is a sesquiterpene lactone isolatedfrom Inula helenium L. Although alantolactone possessesanti-inflammation and apoptosis-induction activities, theunderlying mechanism of anti-cancer effect on humanbreast cancer cells remains largely unknown. In this study,we explored the possibility of alantolactone as an apoptosis-inducing cytotoxic agent using MDA-MB-231 cells asin vitro model. Alantolactone significantly induced itsapoptosis, demonstrated by cell cycle analysis, annexinV-APC/7-AAD double staining and dUTP nick end labeling. Additionally, alantolactone triggered the mitochondrial-mediated caspase cascade apoptotic pathway, whichwas confirmed by increased Bax/Bcl-2 ratio, loss of MMP,release of cytc from mitochondria to cytoplasm, activationof caspase 9/3, and subsequent cleavage of PARP. Z-VADFMKpartially prevented apoptosis induced by alantolactone. Alantolactone provoked the production of ROS, whileNAC (a scavenger of ROS) reversed alantolactone-mediateddepolarization of MMP and apoptosis. Alantolactonemodulated the activities of MAPKs. As expected, cotreatmentwith SB203580, SP600125 or U0126 could reducedthe apoptotic rate. Furthermore, alantolactone decreasedthe protein expressions of p-NF-kB p65 and p-STAT3,increased p-c-Jun level in a dose-dependent manner. Thesefindings suggested that alantolactone possessed anticanceractivity via ROS-mediated mitochondrial dysfunctioninvolving MAPK pathway, and had an effect on the transcriptionfactors of NF-kB, AP-1 and STAT3.

Preparation of Novel Ionic Liquids and Their Applications in Brominating Reaction

Li, Hua,Liu, Juan,Zhu, Jiang,Wang, Hongkai Korean Chemical Society 2011 대한화학회지 Vol.55 No.4

새로운 산성 이온용액인1-(${\omega}$-sulfonicacid)propyl-3-methylimidazolium bromide ([$HSO_3$pmim]Br)과 1-(${\omega}$-sulfonicacid)-butyl-3-methylimidazolium bromide([$HSO_3$bmim]Br)을 제조하였다. 이 화합물들을 브롬화 시약 및 용매로 사용하여 $100^{\circ}C$, 2시간 반응시켰으며, 간단히 상분리에 의해 1,7-dibromoheptane을 95% 수율로 합성하였다. 이 산성 이온용액을 5회 재사용하였으나 수율 감소가 관찰되지 않았다. Novel acidic ionic liquids, 1-(${\omega}$-sulfonicacid)propyl-3-methylimidazolium bromide ([$HSO_3$pmim]Br)and 1-(${\omega}$-sulfonicacid)butyl-3-methylimidazolium bromide ([$HSO_3$bmim]Br), were prepared and used as brominating agents, catalysts and solvents in the synthesis of 1,7-dibromoheptane, respectively. 1,7-dibromoheptan with a yield of 95% was obtained at $100^{\circ}C$ for 2 h by simple phase separation. The acidic ionic liquid [$HSO_3$pmim]Br was recycled for 5 times and the yield of 1,7-dibromoheptane did not decrease remarkably, which indicates that catalysts still maintain good selectivity and activity after recycling. The structure of the acidic ionic liquid [$HSO_3$pmim]Br was characterized with IR, and it was found that [$HSO_3$pmim]Br had stronger acidity than other ionic liquid.

중국 자성재료 산업의 현황 및 발전 전망

Juan Jiang,Ying Li,Huiping Shao,Yang Luo 한국자기학회 2005 韓國磁氣學會誌 Vol.15 No.2

자성재료는 각종 전자품에서 빼놓을 수 없는 중요한 소재로서 가전제품, 컴퓨터, 통신설비, 자동차 및 국방산업과 직결되어 있다. 현재 중국은 각종 자성재료의 생산량이 세계 1위이며 자성재료 생산대국과 산업중심의 역할을 하고 있다. 중국 자성재료의중, 장기 시장 발전 전망이 매우 유망한 바, 중국의 자성재료 생산품의 전세계 에서의 인식은 한층 더 올라갈 것으로 본다. 과학 기술 독창능력, 기술개조와 기업의 관리수준을 반드시 강화시키고 산업구조를 조절하고 생산품의 등급을 한 단계 상승시켜 중국을 자성재료 대국으로부터 강국으로 진입하도록 인도해야 한다는 전략은 지난 11월초 중국 상해에서 개최한 “중국 자성재료 산업 중장기 발전 전략 포럼”에서 제기된 것이다. 본문에서는 이 포럼에서 발표된 내용과 산업계의 통계자료를 바탕으로 거시적인 각도에서 중국 자성체 산업의 전체적인 현황을 분석하였고 희토류 영구자석 특히 중국의 소결과 본드 NdFeB 자석의 산업현황을 소개했고, 희토류 영구자석에 대한 중국의 연구개발 상황을 소개했으며 자성체 산업발전의 전망에 대한 예측과 분석도 수행하였다.

Emotional Regulation and Executive Function Deficits in Unmedicated Chinese Children with Oppositional Defiant Disorder

Wenqing Jiang,Yan Li,Yasong Du,Juan Fan 대한신경정신의학회 2016 PSYCHIATRY INVESTIGATION Vol.13 No.3

ObjectiveaaThis study aims to explore the feature of emotional regulation and executive functions in oppositional defiant disorder (ODD) children. MethodsaaThe emotional regulation and executive functions of adolescents with ODD, as well as the relationship between the two factors were analyzed using tools including Adolescent Daily Emotional Regulation Questionnaire (ADERQ), Wisconsin Card Sorting Test (WCST) and Cambridge Neuropsychological Test Automated Battery (CANTAB), in comparison with attention deficit hyperactivity disorder (ADHD) children without behavioral problem and healthy children; the ADERQ assessed emotional regulation ability and others were used to assess executive function. ResultsaaCompared to normal children, the ODD group displayed significant differences in the scores of cognitive reappraisal, rumination, expressive suppression, and revealing of negative emotions, as well as in the score of cognitive reappraisal of positive emotions. WCST perseverative errors were well correlated with rumination of negative emotions (r=0.47). Logistic regression revealed that the minimum number of moves in the Stocking of Cambridge (SOC) test (one test in CANTAB) and negative emotion revealing, were strongly associated with ODD diagnosis. ConclusionaaChildren with ODD showed emotion dysregulation, with negative emotion dysregulation as the main feature. Emotion dysregulation and the lack of ability to plan lead to executive function deficits. The executive function deficits may guide us to understand the deep mechanism under ODD.

Comparative effect of eldecalcitol and alfacalcidol on bone microstructure: A preliminary report of secondary analysis of a prospective trial

Xiaolin Ni,Juan Feng,Yan Jiang,Li Zhang,Wei Yu,Ou Wang,Mei Li,Xiaoping Xing,Toshio Matsumoto,Weibo Xia 대한골다공증학회 2021 Osteoporosis and Sarcopenia Vol.7 No.2

Objectives: To compare the effect of eldecalcitol and alfacalcidol on skeletal microstructure by highresolution peripheral QCT (HR-pQCT). Methods: This was a substudy of a randomized, double-blind, active comparator trial. Five female osteoporotic patients with 1-year 0.75 ㎍/day eldecalcitol and 5 with 1-year 1.0 ㎍/day alfacalcidol completed HR-pQCT scans before and after treatment were enrolled. Results: Total vBMD [1.67 ± 1.06% (mean ± SD), P = 0.043 versus baseline] and trabecular vBMD (2.91 ± 1.72%, P = 0.043) at the radius increased in eldecalcitol group, while total, trabecular, and cortical vBMD tended to decrease in alfacalcidol group, with a significant reduction in cortical vBMD at the tibia (0.88 ± 0.62%, P = 0.043). Cortical area (1.82 ± 1.92%, P = 0.043) at the radius and thickness (0.87 ± 1.12%, P = 0.043) at the tibia increased in eldecalcitol group, while these parameters decreased with alfacalcidol at the tibia (1.77 ± 1.72%, P ¼ 0.043 for cortical area; 1.40 ± 2.14%, P = 0.042 for cortical thickness). Trabecular thickness at the radius (1.97 ± 1.93%, P = 0.042) and number at the tibia (3.09 ± 3.04%, P = 0.043) increased by eldecalcitol but did not increase by alfacalcidol. Trabecular separation decreased by eldecalcitol (2.22 ± 2.43%, P = 0.043) but tended to increase by alfacalcidol at the tibia. Conclusions: Eldecalcitol has the greater potential to improve cortical and trabecular microstructure at the peripheral bone than alfacalcidol which needs further more studies.

Influence of Psychological Factors in Primary Dysmenorrhea Patients on De qi : a Secondary Analysis of a Randomized Controlled Trial

Hu Ni-juan,Li Chun-hua,Wang Pei,Wu Gui-wen,Ma Liang-xiao,Zhu Jiang 사단법인약침학회 2023 Journal of Acupuncture & Meridian Studies Vol.16 No.1

Background: De qi , the needling sensation, is important in acupuncture treatment. Almost all studies believe that deep needling and manipulation could achieve a significant de qi sensation. However, relatively few studies have examined the effect of psychological factors on de qi, and those that did often reached different conclusions. Objectives: To explore the influence of psychologic factors on de qi in patients with primary dysmenorrhea (PD). Methods: Sixty-eight PD patients with cold and dampness stagnation were randomly allocated to de qi (deep insertion using thick needles, with manipulation, n=17) and non-de qi groups (shallow insertion using thin needles, without manipulation, n=51). Both groups received bilateral needling at Sanyinjiao (SP6) for 30 min. De qi was assessed using the Acupuncture De qi Clinical Assessment Scale (ADCAS). The patients’ acupuncturerelated anxiety and their expectations of the relationship between needle sensation and curative effect were evaluated using a five-point and four-point scale, respectively. Results: Within the de qi group, all patients experienced the de qi sensation, although anxiety levels were unrelated to de qi. Patients’ expectations correlated negatively with de qi timing, and positively with electric sensation. Within the non-de qi group, 59.5% of patients experienced de qi. Between those who experienced it and those who did not, no significant differences were found in anxiety levels, although patients’ expectations differed significantly. Among patients who experienced de qi sensations in the non-de qi group, anxiety and throbbing were positively correlated. Additionally, patients’ expectations correlated positively with de qi intensity, as well as coldness, and numbness. Conclusion: Psychological factors should be considered when studying de qi since PD patients’ expectations could influence the de qi sensation at SP6.

Preparation of Supported CTAB/MCM-41 and CTAB-Nafion/MCM-41 Mesoporous Molecular Sieve and Their Use in the Brominating Reaction

Hu, Guoqin,Li, Hua,Liu, Juan,Zhu, Jiang Korean Chemical Society 2011 대한화학회지 Vol.55 No.4

CTAB 혹은 CTAB-나피온 촉매를 속빈 실리콘 튜브형 MCM-41에 담지시켰다. 이들 촉매들을 XRD, SEM, BET 등으로 분석하였다. 이들을 1,7-heptanediol 의 브롬화 반응 촉매로 사용하였고 다른 촉매들과 비교하였다. 이들 촉매들은 CTAB 보다 효과가 좋았으며 CTAB-Nafion/MCM-41 이 성능이 가장 우수하였다. CTAB or CTAB-Nafion catalyst were successfully supported on siliceous hollow tubular MCM-41 which synthesized by hydrothermal method using CTAB as a single template or CTAB and Nafion-Na as mixed templates. The properties of two kinds of catalysts were characterized with XRD, SEM and adsorption and desorption isotherms of nitrogen and pore diameter distribution, respectively. Then they were applied to catalyze brominating reaction of 1,7-heptanediol, moreover the rate of brominating reaction with different catalysts was compared. The results showed that catalytic activities of CTAB/MCM-41 and CTAB-Nafion/MCM-41 are better than CTAB, and that of CTAB-Nafion/MCM-41 is the best because of its phase-transfer and strong acidity function. The two kinds of catalysts can be separated from the reactive products and recycled.