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Clinical outcomes of vitrified-thawed embryo transfer using a pull and cut straw method
( Joon Gyo Lim ),( Young Tae Heo ),( Seung Gi Min ),( Byeong Yeol Min ),( Sang Jun Uhm ),( Nam Hyung Kim ) 대한산부인과학회 2013 Obstetrics & Gynecology Science Vol.56 No.3
Objective To compare the clinical outcomes of patients with vitrified-thawed embryos transferred using either the 0.25 mL straw method and the pull and cut straw (PNC) method. To evaluate the clinical outcomes of patients with transferred embryos that underwent assisted hatching at the cleaved embryo (day 3) or the blastocyst (day 5) stage. Methods The study population consisted of women who underwent vitrified-warmed embryo transfer between May 2000 and December 2011 and assisted hatching was performed after warming of embryos. Cycles of thawing between assisted hatching treated and non treated groups were compared for survival and pregnancy rates. Results The PNC vitrification method improved survival and pregnancy rates in partial lysed embryos. While assisted hatching did not affect the developmental and clinical pregnancy rates of the vitrified-warmed blastocyst group, it did increase the pregnancy rate of poor quality vitrified-warmed cleaved embryos. Conclusion These results suggest that PNC may increase the number of clinical pregnancies via the vitrification of both cleaved embryos and blastocysts. In addition, selective assisted hatching treatment of embryos that show a poor prognosis after warming may increase the rate of clinical pregnancy.
Joon-Soo Lee,Kyung-Hwan Lee,Sung-Sik Lee,Eun-Soo Kim,In-Ok Ahn,Jun Gyo In 고려인삼학회 2011 Journal of Ginseng Research Vol.35 No.4
Plant leaf cuticle is related to the prevention of moisture loss, transpiration, and diffusion of light reflection. The purpose of this study was to examine the morphological characteristics of ginseng leaves in ginseng plants resistant and susceptible to hightemperature injury (HTl) to be related with the leaf-burning. For the HTI resistant lines Yunpoong, high-temperature injury resistance (HTIR) I, HTIR 2, and HTTR 3, and the HTl-susceptible line Chunpoong, the cuticle densities were 53.0%, 46.2%, 44.9%,48.0%, and 17.0%; the adaxial leaf cuticle layers were 141.3, 119.7, 119.7, 159.4, and 85.0 nm in thickness; the abaxial leaf cuticle layers were 153.6, 165.8, 157.9, 199.6, and 119.4 nm in thickness; and the stomtal lengths were 21.7, 32.4,29.4,30.9, and 21.8 11m, respectively. All of these aspects suggest that HTI resistant lines have higher cuticle density, thickicker adaxial and abaxial leaf cuticle layers, and longer of stomta length than the HTI-susceptible Line, protecting leaves from moisture loss and excessive transpiration under high temperatures to be resistant against the leaf-burning.
( Joon Koo Kang ),( Jin Hong Kim ),( Sun Gyo Lim ),( Kee Myung Lee ),( Sung Jae Sin ) 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1
Objective: Esophageal dilatations with mercury weighted bougies were used for esophageal benign strictures. But, high esophageal restenosis rates and recurrent complications (esophageal perforation, mediastinitis, e.g.) were troublesome. And, many therapeutic modalities (pneumatic dilation, anti-fi brotic drug injection and stent insertion, e.g.) are developing. Therefore, we aimed to develop an appropriate porcine benign esophageal stricture model. Methods and Material: A total of ten mini pigs were sequentially divided into three groups by two, six and two pigs. Two pigs of fi rst group were injected into the four directions of esophagus by NaOH(0.10N) 2ml each. Six pigs of Second group were injected into the four directions of esophagus by NaOH(0.20N) 2ml each. Two pigs of third group were injected into the four directions of esophagus by NaOH(0.15N) 2mleach. We defi ned successful esophageal stenosis as unable endoscopic passage (scope diameter ;10mm) without immediate mortality. Results: Minimal esophageal strictures were noted at the two porcine esophagus of fi rst group. But, endoscopes could be passed through the esophageal stenosis. Moderate to severe esophageal strictures were noted at the all of porcine esophagus of second group. But four pigs (4/6, 80 %) were died within a month due to malnutrition and esophageal perforations. Moderate esophageal strictures were developed at the two porcine esophagus of third group without serious complications. Conclusions: Porcine benign esophageal strictures were developed successfully by NaOH(0.15N) 2ml injection into the four directions of esophagus each.