균 spheroplast 및 large unilamellar vesicle의 식물 protoplast로의 도입

주충노,강철호,이희봉 연세대학교 자연과학연구소 1987 學術論文集 Vol.19 No.-

A. tumefaciens의 식물 protoplast로의 도입을 확인하는 방법으로 방사성 인산(Na_2H^32PO_4)을 함유한 0.8% nutrient broth에서 증식한 Agrobacterium tumefaciens C58를 사용하여 방사성 spheroplast와 방사성 Ti plasmid encapsulated liposome을 만들어 Danus carota protoplast로의 도입을 시도한 결과 다음과 같은 실험 성적을 얻었다. 1. A. tumefaciens에서 추출한 Ti plasmid를 Reverse Phase Evaporation 법으로 phosphatidyl-choline, stearylamine과 함께 Ti plasmid encapsulated liposome을 제조한 결과 17%의 Ti plasmid가 liposome안으로 편입되었다. 2. 방사성 Ti plasmid encapsulated liposome과 Dacus carota root protoplast를 polyethyleneglycol-Ca^2+법으로 처리한 결과 introduction frequency는 38%이었다. 3. A. tumefaciens C58의 spheroplast와 Dacus carota root protoplast를 polyethyleneglycol Ca^2+ 법으로 처리한 결과 introduction frequency가 47.6%였다. 4. 본 연구에서 얻은 실험결과로 부터 liposome법, spheroplast법 모두 Ti plasmid의 식물 protoplast로의 도입은 용이하게 일어나는 것으로 생각된다. It was attempted in the present study to prepare radioactive Ti plasmid to identify the introduction frequency of Ti plasmid encapsulated liposome into Dacus carota root protoplast. Agrobcterium tumefaciens C58 was cultured in 0.8% nutrient broth containing radioactive phosphoric acid(H_3^32PO_4) and the radioactive Ti plasmid wad isolated. Radioactive Ti plasmid was encapsulated into phosphatidyl choline-stearylamine liposomes by reverse phase evaporation technique and the encapsulation frequency was found to be about 17%. Radioactive Ti plasmid encapsulated liposome and Dacus carota root protoplast was treated by polyethyleneglycol Ca^2+ procedure. The introduction frequency was 38%. The spheroplast of A. tumefaciens C38 was prepared from the bacteria cultured in 0.8% nutrient broth containing radioactive phosphoric acid using lysozyme. As much as 47% of the spheroplast were found to be introduced into Dacus carota protoplast.

쇼핑호스트에 대한 의사인간관계가 홈쇼핑 TV에 대한 태도 및 구매의도에 미치는 영향 : 지각된 위험과 지각된 혜택의 매개효과를 중심으로

주지혁,최명일,김봉철 한국방송광고공사 2008 광고연구 Vol.0 No.80

Home-shopping in cable TV is considered as one of the important channels by innovative changes for the shopping. In this process, shopping hosts take a great portion, but they are not weighted in other studies. This study explores various emotions for the shopping hosts in the field of parasocial interaction and examines how those emotions affect to purchase intention. Also, the study can see over how perceived risk, perceived benefit, and attitude for the CATV home-shopping have an impact on the purchase intention. In the result, as parasocial interaction with shopping-hosts would grow, perceived risk for home-shopping is reduced, meanwhile perceived benefit is grown. Perceived risk can mediate purchase intention, and perceived benefit also can moderate attitude for homeshopping and purchase intention. It could mean that consumers can have positive image for CATV home-shopping under the conditions that parasocial interaction with shopping-hosts would be grown. The result can show that CATV home-shopping should attempt to establish parasocial interaction in order to take upper hands against open markets and internet shopping malls.

프레탈정 (실로스타졸 100mg)에 대한 실로스탄정 (한국유나이티드 제약)의 생물학적 동등성

용철순,이경희,최진석,박병주,정세현,김용일,박상만,유봉규,이종달,최한곤 한국병원약사회 2003 병원약사회지 Vol.20 No.2

Bioequivalence of one cilostazol tablets, the Pletaal^(R)(Korea Otsuka Pharmaceutical Co., Ltd.) and Cilostan^(R)(Korea united Pharmaceutical Co., Ltd.), was evaluated according to the guideline of KFDA. Sixteen normal male volunteers(age 20~30 years old)were divided into two groups and a randomized 2×2 cross-over study was employed. After one tablet containing 100㎎ of cilostazol were orally administered, blood was taken at predetermined time intervals and the concentration of cilostazol in plasma was determined with an HPLC method using UV detector. The pharmacokinetic parameters(C_(max), T_(max) and AUC_(t)) were calculated and ANOVA was utilized for the statistical analysis of parameter. The results showed that the differences in C_(max), T_(max) and AUC_(t) between one tablet were 16.08%, 18.88% and 17.57%, respectively. The powers (1-β) for C_(max), T_(max) and AUC_(t) were 85.03%, 83.92% and 80.12%, respectively. Detectable differences(Δ) and confidence intervals were all less than 20%, and confidence interval of all the parameters were also less than 20% at the significance level(α) of 0.05. All of these parameters met the criteria of KFDA for bioequivalence, indicating that Cilostan^(R) tablet is bioequivalent to Pletaal^(R) tablet.

아세클로페낙 연질캡슬(클란자 에스 연질캡슬)의 개발

용철순,이경희,최진석,박병주,정세현,김용일,박상만,배명수,김귀자,김영식,유창훈,강성룡,유봉규,이종달,최한곤 한국약제학회 2004 Journal of Pharmaceutical Investigation Vol.34 No.1

To develop and aceclofenac soft capsule, four preparations with various solubilizers were prepared and their dissolution test was carried out. Among four preparations tested, a preparation with ethanolamine was selected a formula of aceclofenac soft capsule (Clanza S^(™), since it showed the fastest dissolution rate. Bioequivalence of aceclofenac tablet, Airtal^(™)(Dae-Woong Pharmaceutical Co., Ltd.) and aceclofenac soft capsule, Clanza S^(™)(Korea United Pharmaceutical Co., Ltd.) was evaluated according to the guideline of KFDA. Fourteen normal male volunteers (age 20-25 years old) were divided into two groups and a randomized 2×2 cross-over study was employed. After oral administration of one tablet or capsule containing 100 ㎎ of aceclofenac, blood ws taken at predetermined time intervals and the concentration of aceclofenac in plasma wa determined with an HPLC method under UV detector. The pharmacokinetic parameters (C_(max), T_(max) and AUC_(t)) were calculated and ANOVA was utilized for the statistical analysis of parameters using logarithmically transformed AUC_(t), C_(max) and T_(max) between Airtal tablet and Clanza soft capsule were 2.89%, 0.18% and 43.0%, respectively. There were no sequence effects between two formulations in these parameters. The 90% confidence intervals using logarithmically transformed data were within the acceptance range of log(0.8) to log(1.250(e.g.log(0.81) - log(1.23) and log(0.89) - log(1.14)) for AUC_(t) and C_(max), respectively. Thus, the criteria of the KDFA guidelines for the equivalence was satisfied, indicating that Clanza S^(™) soft capsule is bioequivalent to Airtal^(™) tablet.

한·미간의 사고유형과 문화유형의 분석 : 의식구조를 중심으로

申聖澈,黃鳳周,朴義載 충남대학교 어학연구소 1985 언어 Vol.6 No.-

雲吉山 演習林의 自然休養林 造成에 關한 硏究 : 自然環境 및 人文社會環境을 中心으로 With Focus on Natural and Cultural Environment

金峰柱,辛壽哲,尹和榮 동국대학교 생명자원과학대학 연습림 1995 연습림논문집 Vol.- No.4

The study was carried out to investigate the natural and cultural environment, the actual condition for the utilization, the propriety of preparation for recreation forests on experiment forests of Dongguk univ. in Mt. Ungil located at eastern area of Seoul and central parts of Kyunggi Province. The results are as follows: 1. As this area consists of beautiful landscape and famous temples such as Sejungsa and Soojongsa, visitors are increasing every year. Therefore the area showed as much visiting popluation of 5,840 man in the same period as those of 6,279 men during May∼August 1993(vacation season) of the adjacent place, Mt.Chungye which were prepared for natural recreation forest facilities. 2.This area is composed of diverse woody plants;52 Families, 110 Genera, 196 Species by Lee in 1984 and 23 Orders. 45 Families, 209 Species by Kim & Lee in 1981, which deserve to possess almost all the representative decidous broad leaf trees of middle temperate zone in Korean, medicinal plants of 15 Orders, 28 Families, 52 Species, birds 10 Orders, 26 Families, 67 Species including Brown hawk owl as a natural monument, mammalia of 11 Families, and amphibiaㆍreptile of 12 species. 3. As a result of the evalution items for the forest recreation site, Mt. Ungil had a potential area by marking total 67.5 points, summed with 15pts of landscape (suitable), 12pts of flora & fauna(suitable), 14pts of water(possible), 8pts of development condition(unsuitable), 11pts of accessibility (most suitable), and 7.5pts of recreation opportunity (unsuitable), respectively.

소비자의 개인적 성격 특성과 휴먼 브랜드 애착 및 충성도와의 관계 : 빅 파이브(Big Five) 성격모형을 중심으로

김봉철,안주아,최양호 한국방송광고공사 2007 광고연구 Vol.0 No.77

Attachment is a type of strong emotional relationship that people usually first experience as children with their parents; later in life, this attachment routinely develops with other“ target”, such as human brand. Celebrities or entertainers can be considered human brands because they could be professionally managed and they have additional associations and features of a brand. The purpose of this study is to investigate (1) attachment to human brand by consumer demographics such as gender and age, (2) the relationship between personal characters (Big Five model) and attachment to human brand, and (3) attachment to human brand and human brand loyalty(satisfaction, trust, and commitment). The study found that attachment to human brand by gender had a statistically significant difference but there was no significant difference of human brand attachment by age. The Big Five factors had significant correlations with attachment to human brand. The findings also showed that human brand attachment had significant correlations with satisfaction, trust, and commitment, respectively. The findings suggested that attachment to human brand had some positive relationships with consumer personal characters. In the future, we need more segmented approach on finding out consumer’s personal characters so that we could strategically manage human brand. 애착이란 자신과 가까운 사람에 대해서 느끼는 강한 감정적 유대관계로, 최근에는 소비자 행동에까지 확대 적용되고 있다. 특히 유명 연예인과 같은 휴먼 브랜드는 대중들이 이들 휴먼 브랜드에 대해 얼마나 애착을 갖고 있는가에 따라 인기가 좌우된다. 따라서 인구통계적 속성에 따른 휴먼 브랜드에 대한 애착을 살펴보고, 휴먼 브랜드에 대한 애착과 개인적 성격 특성(빅 파이브 성격 변인)의 관계 및 휴먼 브랜드에 대한 애착과 충성도(만족도, 신뢰도, 헌신)의 관계를 분석하였다. 연구결과, 인구통계적 속성에 따른 휴먼 브랜드에 대한 애착은 성별에 따라 통계적으로 유의미한 차이가 있었으나 연령에 따라서는 통계적으로 유의미하지 않았다. 또한 휴먼 브랜드에 대한 애착과 빅 파이브 성격 변인은 전체적으로 유의미한 상관관계를 보였으며 휴먼 브랜드에 대한 애착과 만족도, 신뢰도, 헌신도 모두 유의미한 상관관계가 있었다. 결국 휴먼 브랜드에 대해서는 성별에 따라 애착의 정도가 다르게 나타났으며 휴먼 브랜드에 대한 애착은 개인의 성격 차원과 상관관계가 있음을 알 수 있었다. 이는 브랜드 애착이 장기적으로 소비자와 휴먼 브랜드 관계에서 긍정적인 역할을 할 수 있음을 시사한다. 따라서 휴먼 브랜드의 전략적인 관리를 위해서는 소비자인 대중의 성격을 이해하고 이를 바탕으로 한 세분화된 접근이 필요하다.

早期英語敎育의 實踐方法 硏究 및 敎材開發

申聖澈,黃鳳周,權五良 충남대학교 어학연구소 1982 언어 Vol.3 No.-

최근의 대학 교양영어 교재에 대한 어 · 문학적 고찰

김봉주,신성철,유재익 충남대학교 인문과학연구소 1974 인문학연구 Vol.1 No.1

It is true that the general English textbooks which the Korean colleges and universities use recently have considerably improved for the past thirty years; but still, some of them are edited and published without any good principle. To reflect our attitude toward the compilation of textbooks, we review here some of the readers and laboratory workbooks which are used recently at the ten institutes we have chosen at random. Ten kinds of textbooks that we could collect are for reading, and eight for laboratory. The purpose of learning general English in college is to attain a basic knowledge of English that educated men should have in common and to foster the ability to comprehend the students major textbooks. Someone emphasize hearing or speaking rather than reading English. But in Korea we can not help emphasizing reading. If possible reading and speaking are expected to be well-balanced in learning. To fulfil the above mentioned purpose a suitable textbook of English for college students must be considered from the linguistic and literary point of view. So in this paper we investigate each of the ten colleges' books and found out the following characteristics : (1) The colleges are trying to pay attention to the compilation of better textbooks. (2) The simple style of the books is changing into a comparatively complex one. (3) They show a tendency to avoid taking special literary works only and to include some essays or many varieties of writings. (4) They put emphasis not only upon written English but also upon spoken English. (5) Most of the lessons in those books possess cultural and practical value. (6) Most of the lessons have remarkable notes and exercises. We would like to make following suggestions: (1) Compilers should study every unit more carefully. (2) Textbooks should be systematical. (3) The content of a book had better be a such one that students are interested in. (4) Archaic passages should be replaced with fresh ones. (5) Many varieties of contents should be taken. (6) Compilers and publishers must be conscientious.

피로와 의료이용, 업무상 사고 및 질병결근

장세진,고상백,강동묵,김성아,정진주,이철갑,강명근,현숙정,조정진,차봉석,박종구 大韓産業醫學會 2005 대한직업환경의학회지 Vol.17 No.4

Objectives: This study examined the relationship of fatigue to medical utilization, occupational accident and sickness absence. Subjects and Methods: Data were obtained from the National Study for Development and Standardization of Occupational Stress (NSDSOS Project, 2002~2004). A total of 30,146 employees categorized within the Korean Standard Industrial Classification and the Korean Standard Occupational Classification were recruited. A structured-questionnaire was used to assess the participants' sociodemo-graphics, job-related factors, health-related behaviors, personality trait, self=perceived fatigue (MFS) and the frequency of medical utilization, occupational accident and sickness absence. Data on medical utilization and occupational accident were confirmed from company health records. Chi-square test and logistic regression analysis were used to elucidate the relationship of self-perceived fatigue to medical utilization, occupational accident and sickness absence. Results: In logistic regression analyses, self-perceived fatigue was associated with medical utilization (outpatient), occupational accident and sickness absence although the strength of the associations was higher in the blue collar workers except for medical utilization. For medical utilization, the white collar worker's adjusted odds ratios of outpatient utilization for workers scoring in the third quartile and the highest quartile, compared to workers scoring in the lowest quartile were 1.50 (95% CI: 1.20-1.87) and 2.16 (95% CI: 1.69-2.75), respectively. The blue collar workers' adjusted odds ratios of outpatient utilization for workers scoring in the second (OR: 1.23, 95% CI: 1.06-1.43), third (OR: 1.42, 95% CI: 1.22-1.66) and highest quartile (OR:1.83, 95% CI: 1.55-2.17), respectively, compared to workers scoring in the lowest quartile were statistically significant. However, no associations were found between fatigue and inpatient utilization for either the white or blue collar workers. The blue collar workers' adjusted odds ratios of being injured for workers scoring in the second, third and highest quartile were 1.41 (95% CI: 1.00-2.00), 1.57 (95% CI: 1.10-2.22) and 2.41 (95% CI: 1.69-3.44), respectively, compared to those scoring in the lowest quartile, but, those of the white collar workers were not significant. There was more likely to an increased risk of sickness absence in workers scoring in the second, third and highest quartile compared to those scoring in the lowest quartile in both white and blue collar workers, but the magnitudes of risk were higher in the blue collar workers than the white collars workers. Conclusion: This result suggests that fatigue is a determinant predictor of medical utilization, occupational accident and sickness absence, and that the pattern of risks for the three outcomes differs according to the occupational type. Some limitations of this study and considerations for future study were also discussed.