Macrophage Inflammatory Protein 1α가 T세포성장 및 CD4, CD8 발현에 미치는 영향

최종선,김오환 大韓顎顔面成形再建外科學會 1996 Maxillofacial Plastic Reconstructive Surgery Vol.18 No.1

Macrophage inflammatory protein (MIP)-1αcytokine which produces wide range of bioctivities such as proinflammatory, immunomodulatory, and hematopoietic modulatory actions. To determine whether MIP-1αacts as a negative regulator on the functions of lymphocyte, [³H]-thymidine incorporation test and flow cytometric analysis were performed by using human tonsil T cell, human peripheral blood T cell, and murine cytolytic T lymphocyte (CTL) line CTLL-2, The mresults were as follow. 1. When human tonsil T lymphocytes were stimulated with anti-CD3 monoclonal antibody (mAb), rate of T cell proliferation was about four times increased. 200ng/ml of MIP-1α inhibited anti-CD3 mAb-mediated T cell growth as much as 60% (P<0.05)> 2. The suppression of human peripheral T cell proliferation produced by MIP-1αwas dramatic, but variable among T cells derived from different individuals (40%∼90%). 3. MIP-1αinhibited the mproliferation of murine CTL line CTLL-2 as much as 75%(P<0.001). 4. WHen the MIP-1αwas added to human peripheral T cell, cell proporation of CD4+ helper T cell and CD8+ CTL were not noticeably affected. The expression level of CD4, not of Cd8, however, was down regulated by MIP-1αtreatment (27%∼82%).

떨어진 코끝의 코성형술 : 분류 및 교정 Classification & Correction

이백권,송진경,이종원,안상태,오영환 大韓成形外科學會 2004 Archives of Plastic Surgery Vol.31 No.2

고 무기질 음료(π-water) 섭취시 럭비선수들의 근력, 근 지구력 및 유산소성 운동능력에 미치는 영향

손두옥,선우섭,윤우상,유재충,박영진,오종환 경희대학교 사회체육연구소 1996 體育科學論叢 Vol.- No.9

Most of elite sports player during the sports competing and trainning have used the mineral supplements to enhance on the human performance and, also a number of researcher have recommended the mineral supplements of RDA(Recommended Dietary Allowance) for them. However, It has not exactly known to the scientific interpretation and evidences of relationship between human performance and mineral. Thence, The purpose of this study was to prove whether the mineral supplements of high RDA could enhance the sport player's performance or not The subjects of this study were composed of sixteen Rugby football players(experimental group=8, high density multimineral water intake for 30days; control group=8, placebo intake)in K-H University. Both of groups were tested three times(pre=before intake, mid=after 15days supplements, post=after 30days supplments) about maximum anaerobic power(MAnp) and maximum oxygen uptake by bicycle ergometer(868), muscle strength and muscle endurance by isokinetic Cybex system(350). The statistical analysis employed this study was t-test by statistic 512+ program in machintosh computer. The result of this study were appeared that the difference in control and experimental group signified that the experimental group was high more than the control group in left knee extensor's peak torque at mid-test(P<.05), in left knee extensor's total work(%BW) at mid-test(P<.05), in left extensor's average power and average power(%BW) at mid-test(P<.05). Relative maximal oxygen uptake(㎖/LBMkg/min) in experimental group was higher than control group after 30 days the mineral supplements. These results suggest that the high density multimineral water intake should influence partially rugby football player's performance by the increment of muscle endurance and maximal oxygen uptake.

Regulation of Vacuolar H<SUP>⁢</SUP>-ATPase c Gene Expression by Oxidative Stress

Whan-Jong Kwak,Seong-Mook Kim,Min-Sung Kim,Jung-Hoon Kang,Dong-Jin Kim,Ho-Shik Kim,Oh-Joo Kown,In-Kyung Kim,Seong-Whan Jeong 대한생리학회-대한약리학회 2005 The Korean Journal of Physiology & Pharmacology Vol.9 No.5

By using differential display, we identified one of the genes encoding the multi-subunit complex protein V-ATPase, c subunit gene (ATP6L), and showed alterations of the gene expression by oxidative stresses. Expression of the ATP6L gene in Neuro-2A cells was increased by the treatment with H<SUB>2</SUB>O<SUB>2</SUB> and incubation in hypoxic chamber, implying that the expression of the ATP6L gene is regulated by oxidative stresses. To examine mechanisms involved in the regulation of the gene expression by oxidative stresses, the transcriptional activity of the rat ATP6L promoter was studied. Transcription initiation site was determined by primer extension analysis and DNA sequencing, and promoter of the rat ATP6L and its deletion clones were constructed in reporter assay vector. Significant changes of the promoter activities in Neuro-2A cells were observed in two regions within the proximal 1 kbp promoter, and one containing a suppressor was in ⁣195 to ⁣220, which contains GC box that is activated by binding of Sp1 protein. The suppression of promoter activity was lost in mutants of the GC box. We confirmed by electrophoretic mobility shift and supershift assays that Sp1 protein specifically binds to the GC box. The promoter activity was not changed by the H<SUB>2</SUB>O<SUB>2</SUB> treatment and incubation in hypoxic chamber, however, H<SUB>2</SUB>O<SUB>2</SUB> increased the stability of ATP6L mRNA. These data suggest that the expression of the ATP6L gene by oxidative stresses is regulated at posttranscriptional level, whereas the GC box is important in basal activities of the promoter.

Regulation of Vacuolar $H^+-ATPase$ c Gene Expression by Oxidative Stress

Kwak, Whan-Jong,Kim, Seong-Mook,Kim, Min-Sung,Kang, Jung-Hoon,Kim, Dong-Jin,Kim, Ho-Shik,Kown, Oh-Joo,Kim, In-Kyung,Jeong, Seong-Whan The Korean Society of Pharmacology 2005 The Korean Journal of Physiology & Pharmacology Vol.9 No.5

By using differential display, we identified one of the genes encoding the multi-subunit complex protein V-ATPase, c subunit gene (ATP6L), and showed alterations of the gene expression by oxidative stresses. Expression of the ATP6L gene in Neuro-2A cells was increased by the treatment with $H_2O_2$ and incubation in hypoxic chamber, implying that the expression of the ATP6L gene is regulated by oxidative stresses. To examine mechanisms involved in the regulation of the gene expression by oxidative stresses, the transcriptional activity of the rat ATP6L promoter was studied. Transcription initiation site was determined by primer extension analysis and DNA sequencing, and promoter of the rat ATP6L and its deletion clones were constructed in reporter assay vector. Significant changes of the promoter activities in Neuro-2A cells were observed in two regions within the proximal 1 kbp promoter, and one containing a suppressor was in -195 to -220, which contains GC box that is activated by binding of Sp1 protein. The suppression of promoter activity was lost in mutants of the GC box. We confirmed by electrophoretic mobility shift and supershift assays that Sp1 protein specifically binds to the GC box. The promoter activity was not changed by the $H_2O_2$ treatment and incubation in hypoxic chamber, however, $H_2O_2$ increased the stability of ATP6L mRNA. These data suggest that the expression of the ATP6L gene by oxidative stresses is regulated at posttranscriptional level, whereas the GC box is important in basal activities of the promoter.

Ancient Mitochondrial DNA Analyses of Ascaris Eggs Discovered in Coprolites from Joseon Tomb

Chang Seok Oh,Min Seo,Jong Ha Hong,Jong-Yil Chai,Seung Whan Oh,Jun Bum Park,Dong Hoon Shin 대한기생충학열대의학회 2015 The Korean Journal of Parasitology Vol.53 No.2

Analysis of ancient DNA (aDNA) extracted from Ascaris is very important for understanding the phylogenetic lineage of the parasite species. When aDNAs obtained from a Joseon tomb (SN2-19-1) coprolite in which Ascaris eggs were identified were amplified with primers for cytochrome b (cyt b) and 18S small subunit ribosomal RNA (18S rRNA) gene, the outcome exhibited Ascaris specific amplicon bands. By cloning, sequencing, and analysis of the amplified DNA, we obtained information valuable for comprehending genetic lineage of Ascaris prevalent among pre-modern Joseon peoples.

수은중독의 임상증상과 생물학적 폭로지표간의 연관성에 관한 조사 연구

吳惠淑,金光鍾,車喆煥 고려대학교 의과대학 1990 고려대 의대 잡지 Vol.27 No.1

This research focused on the association between clinical symptoms of mercury poisoning and mercury concentration in urine and blood. A total of 100 manual workers and 55 office workers in a fluorescent lamp manufacturing factory were selected and history-taking and physical examination were done from September 1988 to Octorber 1989. The results were as follows : 1. The urinary mercury concentration of manual workers in average was 125.9 ㎍/l(5.0-469.0㎍/l), which showed 10 times higher than that of the office workers, and the blood mercury concentration of manual workers in average was 6.3 ㎍/l00ml(0.2 -60.2 ㎍/l00㎖), which was 6.6 times higher than that of office workers. 2. The frequency of mercury poisoning symptoms appeared was higher in manual workers than in office workers. Among the symptoms, anorexia(33.0%), shyness(15.0%), gingivitis(11.0%) and stomatitis(11.0%) showed significantly higher in manual workers than in office workers. In every item of symptoms, the manual workers showed a higher average of urinary and blood mercury concentration than that of the office workers 3. The average urinary mercury concentration of symptom-holders(shyness, loss of memory and anorexia) among manual workers was higher than that of non-symptom-holders. The average blood mercury concentration of symptom-holders(excessive sweating and blood mercury concentration than that of the office workers. anxiety, loss of weight) was higher than that of non-symptom-holders. 4. Shyness, loss of memory, loss of patience, anorexia, loss of weight, dyspepsia, excessive sweating, respectively showed increase of the number of symptom - holders according to the urinary mercury level. However, there was no increase of the number of symptom-holders according to the blood mercury level.

일본잎갈나무 , 잣나무 , 신갈나무 임분 토양수 성질의 계절적 변이

오종민,진현오,손요환,주영특,정덕영,류성렬 한국환경농학회 2000 한국환경농학회지 Vol.19 No.1

토양수 내 이온 농도의 계절적 변이를 구명하기 위하여 경기도 광주군 퇴촌면에 위치한 26년생 일본잎갈나무, 잣나무, 신갈나무 임분의 O층, A층, B층에 zero tension lysimeter를 설치하고 1996년 9월부터 1999년 6월까지 토양수를 채취하여 분석하였다. 10월과 11월에 토양수 내 K^+, Mg^2+, Cl^-, NO_3^-등의 농도가 증가하였는데, 이는 가을철 낙엽 및 낙지의 유입에 의한 것으로 보이며, 3월과 4월에 Na^-, Ca^2+, K^+, Mg^2+ 등의 농도가 증가하는 것은 봄철에 빈번하게 발생하는 황사현상에 기인하는 것으로 사료된다. 또한 토양수 내 이온 가운데서 K^+의 농도가 연중 계절별로 가장 크게 변하는 것으로 나타났으며, 토양수 내 CI 농도는 K^+, Mg^2+ 농도와 양의 상관관계를 나타내었다. 단변량 시계열 분석 결과 연구기간 동안 토양수 pH는 지속적으로 감소하는 반면 Al^3+과 K^+농도는 증가하는 경향을 나타내었다. 이와 같은 경향이 지속될 경우 O층 토양수 내 Al^3+농도가 식물체에 피해를 줄 수 있는 최소농도 (0.018 meq/1)에 도달하려면 수종에 따라 차이는 있으나 10-20년이 소요될 것으로 추정되었다. To investigate seasonal variations of soil solution chemistry, samples were collected from zero tension lysimeters in O, A, and B horizons of 26-year-old Larix leptolepis. Pinus koraiensis and Quercus mongolica stands in Kwangju, Kyunggi Province from September, 1996 through June. 1999. Potassium, Mg^2+, Cl^-, and No_3^- concentrations increased in October and November concurrent with inputs of fresh litterfall and twigs. Sodium, Ca^2+, K^+, and Mg^2+ concentrations increased in March and April concurrent with the yellow sand effect. Potassium concentration showed the highest variation among ions, and Cl^- concentration showed positive correlations with K^+ and Mg^2+ concentrations. Soil solution pH decreased while Al^3+ and K^+ concentrations increased during the study period. If our data reflects long term trends. then Al^3+ concentration in the O horizon will reach the toxic level (0.180 meq/1) within 10-20 years depending on species

Variation of storage protein subunits in soybean germplasm, and protein and free amino acid content of the identified variants

Jong Min Ko,Jin Whan Lee,Hyun Tae Kim,Won Young Han,Ki Won Oh,In Youl Back,Geung Joo Lee,Geum Yong Par,Je Kyu Kim 한국육종학회 2007 한국육종학회 학술발표회 발표요지 Vol.39 No.1

문경지역에 분포하는 변성 염기성암과 변성 퇴적암에 대한 백악기 화강암의 열변성작용

오창환,김성원,김종섭 全北大學校 基礎科學硏究所 1994 基礎科學 Vol.16 No.-

문경지역의 변성 퇴적암류와 변성 염기성암류는 북측과 서측에서 대규모로 관입한 백악기 화강암체에 의해 변성작용을 받았다. 변성 염기성암내의 광물군은 화강암체로부터 멀어지면서 각섬석+사장석, 각섬석+사장석+녹염석 그리고 각섬석+사장석+녹염석+녹니석의 광물군으로 변화한다. 이들 변성 염기성암내의 각섬석과 사장석은 변성정도와 암석성분에 따라 매우 다양한 성분의 변화를 보여준다. 변성 염기성암과 함께 산출되는 변성 퇴적암은 대체적으로 백암기 화강암의 접촉부에서 멀어질수록 천매암질 편암 그리고 천매암 혹은 점판암으로 전이한다. 변성 퇴적암의 광물군은 백악기 화강암 근처에서는 근청석+흑운모+백운모+녹니석 혹은 홍주석+흑운모+백운모+녹니석 (근청석대)이며 접촉부에서 멀어지면서 흑운모+백운모+녹니석 광물군(흑운모대)를 거쳐 녹니석+백운모의 광물군(녹니석대)으로 전이한다. 문경지역의 변성 니질암으로부터 계산된 근청석대의 온도는 480∼580℃이다. 반경이 대략 4 km 이상인 연구지역의 백악기 화강암의 열변성작용에 관한 이론적인 연구로부터 화강암 관입체가 주변암에 미치는 변성정도는 주로 관입체의 온도에 의해 결정되며 화성암체의 크기에 의해서는 큰 영향을 받지 않는다는 사실이 인지되었다. 문경지역의 백악기 화강암이 2 km 반경내에 500 ℃ 이상의 변성작용을 야기시킨다는 이론적인 연구결과는 문경지역의 암석학적인 연구와 잘 일치한다. 결론적으로 문경지역의 백악기 화강암은 홍주석과 근청석을 수반하는 저압접촉 변성작용을 일으켜 접촉부에서 1∼2 km 이내의 암석에는 각섬암상의 변성작용을 그리고 2∼5 km 떨어진 암석에는 녹염석-각섬암상과 녹색편암상의 변성작용을 야기시켰다. Metabasites and metapelites in the Mungyong area were intruded by Cretaceous granites with radius of 4∼8 km. As the distance from granite body increases, the mineral assemblage of metabasite changes from amphibole+plagioclase through amphibole+plagioclase+epidote to amphibole+plagioclase+epidote+chlorite. The compositional variations of amphibole and plagioclase according to the change of metamorphic grade and bulk rock comositions are very complex. Towards the Mungyong Cretaceous granite body, the mineral assemblage of metapelite changes from chlorite+muscovite(chlorite zone) through biotite+chlorite+muscovite(biotite zone) to andalusite+biotite+muscovite±chlorite or cordierite+biotite+muscovite±chlorite(cordierite zone). The estimated metamorphic conditions of cordierite zone are 480∼580℃. 1.5∼3.3kb. The theoretical study on the thermal metamorphism caused by the Cretaceous granite with radius longer than 4 km in the Mungyong area suggests the followings: The degree of metamorphism is mainly determined not by the size of granite body but by the temperature of granite intrusion; The country rocks within 2 km from Creataceous granite have undergone metamorphism with temperature higher than 500℃, which is consistent with the petrological study in the Mungyong area. Mungyong Cretaceous granite caused a low P/T thermal metamorphism to the country rocks; the amphibolite facies metamorpism to the country rocks within 1∼2 km from the granite body and the epidote-amphibolite and greenschist facies metamorphism to the country rocks within 2∼5 km.