Helicobacter pylori 항원을 이용한 면역우유 생산 및 항체특성에 관한 연구

배만종,김수정,예은주,김병기,박창호,김미경 경산대학교 생명자원개발연구소 2003 생명자원과 산업 Vol.7 No.-

본 연구는 위염, 위궤양, 위림프종 및 위암과 같은 소화기 질환의 원인균으로 알려진 Helicobacter pylori균을 항원으로 하여 젖소에 면역시킨 후 생산된 우유의 anti-H. pylorigkdcp의 생성능을 검토하고, 백신투여량과 항체 생성과의 관계, 항원 항체의 특이성, H. Pylori균 응집력, 항체의 산과 열에 대한 안정성, 그리고 백신투여가 젖소에 미치는 영향을 알아보았다. 1. 백신 투여량에 따른 혈청과 유청내의 anti-H. pylori항체의 함량은 10㎖, 20㎖, 30㎖ 백신투여 모든 군에서 대조구에 비해서 높은 양의 항체 생성을 확인하였다. 유의적인 차이는 없었으나 20㎖투여가 항체 생성이 가장 많은 것으로 조사되었다. 백신 투여량에 따른 유청내의 anti-H. pylori항체 생성량은 혈청에서 나타난 결과와 유사한 양상으로 형성되었다. 2. Anti-H. pylori항체의 SDS-PAGE에 의한 분자량측정 결과 heavy chain은 50kDa정도, light chain은 24kDa정도로 확인 되었다. 3. H. pylori항원 단백질의 분자량측정 결과 12개의 band가 형성되었다. Anti-H. pylori의 항원 특이성을 알아보기 위해 western bloting을 한 결과 혈청, 혈청정제, 유청, 유청 정제 모두 7개의 항원성 물질을 확인할 수 있었고, 주 항 원성 물질은 분자량이 97, 66, 34kDa 이었다. 4. 응집반응결과 유청속의 anti-H. pylori항체가 H. pylori균에 대해 1/10의 응집가를 나타내었다. 5. Anti-H. pylori항체의 산·알칼리에 대한 안정성 실험에서는 pH 5 ∼ pH 10 범위에서 안정한 상태로 100%의 활성을 나타내었다. 6. Anti-H. pylori항체의 열에 대한 안정성 실험에서는 60℃에서 60분간 안정한 상태를 보였고, 70℃에서도 비교적 안정한 상태였으나 60분 경과후 40%정도 활성이 감소하였다. 80℃에서는 4분간 처리했을 때 77%의 활성을 유지하였고, 100℃에서도 1분간은 비교적 안정한 상태였다. 7. 백신투요로 인하여 유량이 12% 감소하는 경향을 나타냈고, 최장 1주일 정도 지나면서 회복되었다. 8. 백신투여 후 젖소의 체온을 측정한 결과 대조구에 비해 정상적인 범위 내에서 체온이 상승하는 경향을 나타내었다. This study has been to examine bio-function of anti-H. pylori antibodies of milk produced from cows immune with antigen germ of Helicobacter pylori and search the relation between vaccine dosage volume and antibody formation, peculiarity of antigen antibody, cohesion of H. pylori germ, stability about add and heat of antibody, and impact of vaccine dosage on cows. The content of serum and Anti-H. pylori antibody within whey in accordance with vaccine dosage volume has confirmed the formation of high-quantified antibody compared to the controlled conditions in all groups vaccine dosages of 10㎖, 20㎖, and 30㎖. It has been turned out that the antibody was farmed most in 20㎖ dosage on while there was no attention difference. The molecular weight of Anti-H. pylori antibody measured by SDS-PAGE were turned out as about 50kDa in the heavy chain and about 24kDa in the light chain. 12 bands were formed as the result of measured molecular weight of antibody protein. The western blotting was performed in order to examine the antigen peculiarity of Anti-H. pylori that all 7 antigen substances including serum, serum refining, whey and whey refining could be confirmed and the main antigen substances were 97, 66, 34kDa of molecular weight. As a result of cohesive response Anti-H. pylori antibody in whey showed 1/10 cohesive rate about H. pylori germ. In stability test about acid and alkali of antibody there was 100% activated in the range of pH 5-pH 10. In stability test about heat it showed stable condition in 60℃: for 60 minutes and comparatively stable condition in 70℃, but reduced activation to 40% after 60 minutes. It maintained 77% activation in 80℃ for 4 minutes and comparatively stable in 100℃ for I minute. It was inclined to reduce to 12% of flow caused by vaccine injection, but recovered after about maximum 1 week. In measurement of body temperature of cows after vaccine injected, it was inclined to rise with the normal scope in comparison with the controlled conditions.

초등교육용 온라인 멀티미디어 코스웨어 개발을 위한 설계 전략

배종수,최성우 서울교육대학교 과학교육연구소 1997 科學과 數學敎育 論文集 Vol.23 No.-

직류전위차법에 의한 피로균열 진전속도 측정시스템의 제작

배성인,차정환,김종섭,최수준 國立 昌原大學校 産業技術硏究所 1995 産技硏論文集 Vol.9 No.-

Automatic fatigue crack length measuring system using DC electrical potential method and the system control program for automatic fatigue testing under random load condition were made in this study. And using these system and control program, fatigue tests were executed under constant and random load condition. As the result, the propagation of crack in random loading can be represented Paris equation and log normal probability function. But, constant and random load test show different crack propagation properties.

數學 및 科學 敎育 授業現場에서의 Portfolio의 適用 : 基礎 硏究(Ⅰ) Preliminary Study(Ⅰ)

裵鐘洙,徐熹愛 서울교육대학교 과학교육연구소 1995 科學과 數學敎育 論文集 Vol.21 No.-

The term portfolio has become a popular word in areas of educational research. Unfortunately, the background of educational theories and philosophical perspectives has not been clearly established to support the effectiveness of the use of portfolios in classrooms. In this study, we have reviewed the definitions of portfolio, have analyzed the effectiveness of portfolio in mathematics and science education, and have discussed suggestions and guidelines for teachers how to use portfolios in their classroom. Further, from this study, we proposed that portfolio is grounded in learning process which aligned with Constructivism and individualized instruction. Learning characteristics of students in portfolio classroom include self-refelction upon their own learing, continuous growth in learning, and self-motivated learning. Students also improve academic, personal management, and teamwork skills in the process of learning. Particularly, the use of portfolio in science and mathematics classrooms enhance student's problem solving skill, creativity, reasoning skill, scientific thinking skill, and ability to apply knowledge to real world. It was recommended that teachers and teacher educators need to understand the nature of portfolio in order to effectively implement portfolios in classrooms. Changes in current classroom culture toward portfolio approach would stimulate more effective student learning and improve science and mathematics education in the country.

肝吸蟲에 대한 Albendazole의 殺蟲效果에 관한 實驗的 硏究

裵平元,金洙鎭,林漢鍾 고려대학교 의과대학 1984 고려대 의대 잡지 Vol.21 No.3

Newly developed albendazole(zentel®) is a broad spectrum anthelmintic which high activity against intestinal nematodas as well as trematodes and cesstodes infections. The present study was undertaken to evaluato in vitro and in vivo activities of albendazoles against adult worms of clonorchis sinensis. The morphological change of C. sinensis obtained from experimentally infected rats treated with various dosages of albendazole were studied by means of scaning and transmission electron microscope. The results are as follows: 1. The adult worms of C. sinensis were incubated in the medium containing albendazole with various concentrations. By 24 hours after incubation, none of the worms were dead in concentrations of 0.01μg/ml of albendazole. 2. In order to evaluate the effect of albendazole in vivo, experimentally infected rats with 50 metacencariae of C. sinensis each were treated orally with various dosages of albendazole. In the result, anthelmintic effect was confirmed at the dosage of albendazole with 2×25㎎/㎏×2 days (total amounts 100㎎/㎏). 3. Macrocopital examination was to the clonorchis worms which were collected from the bile ducts of the rats autopsied at 24 hours after treatment with albendazole. The lack of intestinal contents was observed in most of worms from the groups treated with 1×200 ㎎/㎏ and 2×25 ㎎/㎏×2 or 4 days, and the elongation of the anterior portion of the worm body was also observed n the worms from the groups treated with 2×25 ㎎/㎏×2 or 4 days. 4. In the scanning electron microscopic observation, many blebs were fromed on the surface of the damaged tegument of the worms at 24 huors after treatment with albendazole(1×200 ㎎/㎏ and 2×25 ㎎/㎏×2 or days). 5. The transmissions electron microscopic observation indicated that degeneration of mitochonriae and destruction of tegumental syncytium of the worms at 24 hours after treatment with albendazole(1×200 ㎎/㎏ and 2×25㎎/㎏ or 4 days). Inaddition to the findings the degeneration of the intestinal lamellae in the caeca was also observed in the worms from the groups treated with same dosages of albendazole.

서울敎育大學敎 學內 電算網 構築 方案에 관한 硏究

裵鍾洙,洪命熹,張豪演 서울교육대학교 과학교육연구소 1994 科學과 數學敎育 論文集 Vol.20 No.-

計算機를 活用하여 問題 解決 指導를 위한 資料 開發에 대한 硏究

배종수 서울교육대학교 과학교육연구소 1997 科學과 數學敎育 論文集 Vol.23 No.-

Cation基의 분포가 다른 Cationic Polyacrylamides(C-PAM)의 펄프에의 흡착성 : 흡착시간의 영향에 대하여

朴成培,趙鍾洙,尹承洛,田中浩雄 진주산업대학교 농업기술연구소 1999 農業技術硏究所報 Vol.12 No.-

The adsorption of two cationic polyacrylamides (C-PAM), with similar molecular weights (MW) 5.3·10^5 and 5.1·10^5 and charge density (CD) 1.19 and 1.25 meq/g but with different distribution of cationic groups respectively has been studied. There are two kinds of substrates ; one is cellulose fiber without fine fraction, and the other is cellulose fibers with fine fraction. At the stirring speed used, the adsorption of these C-PAMs onto cellulose fibers was rapid regadless of cationic distribution, and the adsorption amount of C-PAM with homogeneous cationic distribution was a little greater than that with heterogeneous cationic distribution. This small difference is probably due to the existence of irregularities on the surface of cellulose fibers and the greater diffusion of the C-PAM with homogeneous cationic distribution. That only a small difference is observed is due primarily to the strong attraction between opposite charges. The adsorbed amounts of C-PAM gradually increased both for cellulose fibers, most rapidly for Cellulose fibers with fine fraction and most slowly for Cellulose fibers without fine fraction. These results can be attributed to the porous nature of the cellulose fibers and the adsorption area of the fine fraction.

타액 오염하에서 수복방법에 따른 컴포머의 미세누출에 관한 연구

공석배,김종수,유승훈 대한소아치과학회 2007 大韓小兒齒科學會誌 Vol.34 No.1

소아 환자의 인접면 우식을 치료할 때 컴포머는 불소를 방출하는 재료로 유용하게 사용될 수 있다. 하지만 구강내 환경은 항상 습윤한 상태로 타액은 컴포머와 치질 사이의 접착을 방해할 수 있다. 수복시에 타액이 게재하게 되면 미세누출이 발생할 가능성이 높아지게 되며 그로 인해서 수복의 실패가 일어날 수 있다. 이에 본 연구에서는 컴포머를 수복할 시에 타액의 영향과 수복 방법에 따른 미세누출 정도를 평가하기 위해서 시행하였다. 컴포머로서 Dyract AP^(®)(Dentsply. Germany)를 사용하였으며 Dentin bonding agent로는 Prime and Bond^(®) NT (Dentsply, Germany)를 사용하였고. 광중합을 위해서 Elipar Trilight (3M ESPE, USA)를 사용하였다. 구강내 환경을 재현하기 위해서 saliva pool을 제작하였다. 소구치 2개를 인접하여 시편을 제작한 후에 2급 와동을 형성하여 수복 환경을 다르게 하여 컴포머를 충전한 후에 500회 thermocycling하였다. 그 후 0.5% methylene blue용액에 24 시간 동안 담근 후에 실체 현미경을 통해서 교합면과 치은면에서의 미세누출 정도를 측정하였다. Kruskal-Wallis Test와 Mann-Whitney Test를 이용하여 각 군간 유의성을 검정하여 다음과 같은 결과를 얻었다. 1. 교합면에서의 각 군간 통계학적 차이는 없었다(p>0.05). 2. 치은면에서 Oraseal^(®)을 이용하여 수복한 3군이 타액 오염을 시키지 않은 4군과 통계학적 차이가 없었다(p>0.05). 3. 치은면에서 1군과 2군 사이에는 통계학적 차이가 없었다(p>0.05). 4. 치은면에서 Oraseal^(®)을 이용하여 수복한 3군이 1,2군보다 통계학적으로 더 낮은 미세누출 정도를 보였다(p<0.05). Compomer that release fluoride could be used on proximal caries of child effectively. But oral cavity is always wet, so saliva inhibits bonding of tooth and compomer. When the saliva exist on bonding, it can be occured microleakages. The purpose of this study was to evaluate the influence of salivary contamination on compomer restoration and degree of microleakage according to restoration methods. Dyract AP^(®) and prime and bond^(®) NT was applied by the manufacture s instructions. Elipar Trilight was applied for light curing. Saliva pool was made for reconstruction of oral cavity. Two premolar was embedded in acrylic resin. After class II cavity preperation, Dyract AP^(®) was restored under several condition, the specimen was thermocycled 500 times with 30 second dwell time. 0.5% methylene blue was used for microleakage test. Micoleakage was measured by the ratio of the infiltration length to occlusal and gingival side interface. Data were analyzed statistically using Kruskal Wallis Test, Mann-Whitney Test. The Result were as follows; 1. In occlusal side, there were no statistical differences. 2. In gingival side, there were no statistical differences in Group III(ContactMatrix^(TM), Rubber dam, Oraseal^(®), Group IV (No saliva contamination). 3. In gingival side, there were no statistical differences in Group I(ContactMatrix^(TM)), II(ContactMatrix^(TM), Rubber dam). 4. In gingival side, there were statistical differences in Group I(ContactMatrix^(TM)), II(ContactMatrix^(TM), Rubber dam).

위에서 발생한 원발성 순수편평상피암 1예

이수형,배용목,권균홍,조용건,이현승,김규종 고신대학교의과대학 2007 고신대학교 의과대학 학술지 Vol.22 No.2

A case of pure squamous cell carcinoma of the stomach in a 41-year-old female is reported. Its pathogenesis is still unknown and epidermoid carcinomas of the stomach without a glandular cell component are a rare tumor described in only about 20 patients since 1905. In Korea, six patients have reported to date. In this case upper gastrointestinal endoscopy showed a large ulceroinfiltrating mass with marginal irregularities in the lower body of the stomach. Histologic finding of biopsy specimen was squamous cell carcinoma. Abdominal computerd CT showed metastatic invasions to the spleen, abdominal wall and small bowel loop.