Adipose-Derived Stem Cell Exosomes Promoted Hair Regeneration

Wu Jinyan,Yang Quyang,Wu Sainan,Yuan Ruoyue,Zhao Xiansheng,Li Yue,Wu Wenyu,Zhu Ningwen 한국조직공학과 재생의학회 2021 조직공학과 재생의학 Vol.18 No.4

Background: Some scholars have found that dermal papilla spheroid–derived exosomes could promote the development of hair follicles. However, whether adipose-derived stem cell exosomes (ADSC-Exos) have a similar effect on hair growth has not been determined yet. Thus, the purpose of this article was to detect whether ADSC-Exos could promote hair regeneration. Methods: Adipose-derived stem cells (ADSCs) were isolated from 6-week-old C57BL/6 mice. Then, ADSC-Exos were isolated from the ADSCs. Western blotting was used to detect specific exosome markers. The particle size and distribution of the exosomes were analyzed by NanoSight dynamic light scattering. A total of 12 nude mice were randomly divided into two groups (n = 6 each): the ADSC-Exos group and the control group. For the control group, a mixture of freshly isolated dermal cells (DCs) and epidermal cells (ECs) was grafted. For the ADSC-Exos group, a mixture of DCs, ECs, and 50 μg/ml of ADSC-Exos was grafted. Gross evaluation of the hair regeneration was carried out 2–3 weeks after the transplantation, and the graft site was harvested for histology at the third week. Results: The existence of exosomes derived from ADSCs was evidenced by CD63, ALX1, and CD9 expression. Two or three weeks after the grafting, the number of regenerated hairs in the ADSC-Exos group was higher than that in the control group (p < 0.001). Histologically, the terminal hairs were remarkable in the ADSC-Exos group, whereas the hair follicles observed in the control group were comparatively immature. The ADSC-Exos group had a higher number of regenerated follicles than the control group (p < 0.001). In addition, we found that the skin tissues in the ADSC-Exos group had higher PDGF and vascular endothelial growth factor expressions and lower transforming growth factor beta 1 levels than those in the control group. Conclusion: Our results indicated that ADSC-Exos could promote in vivo hair follicle regeneration. Background: Some scholars have found that dermal papilla spheroid–derived exosomes could promote the development of hair follicles. However, whether adipose-derived stem cell exosomes (ADSC-Exos) have a similar effect on hair growth has not been determined yet. Thus, the purpose of this article was to detect whether ADSC-Exos could promote hair regeneration. Methods: Adipose-derived stem cells (ADSCs) were isolated from 6-week-old C57BL/6 mice. Then, ADSC-Exos were isolated from the ADSCs. Western blotting was used to detect specific exosome markers. The particle size and distribution of the exosomes were analyzed by NanoSight dynamic light scattering. A total of 12 nude mice were randomly divided into two groups (n = 6 each): the ADSC-Exos group and the control group. For the control group, a mixture of freshly isolated dermal cells (DCs) and epidermal cells (ECs) was grafted. For the ADSC-Exos group, a mixture of DCs, ECs, and 50 μg/ml of ADSC-Exos was grafted. Gross evaluation of the hair regeneration was carried out 2–3 weeks after the transplantation, and the graft site was harvested for histology at the third week. Results: The existence of exosomes derived from ADSCs was evidenced by CD63, ALX1, and CD9 expression. Two or three weeks after the grafting, the number of regenerated hairs in the ADSC-Exos group was higher than that in the control group (p < 0.001). Histologically, the terminal hairs were remarkable in the ADSC-Exos group, whereas the hair follicles observed in the control group were comparatively immature. The ADSC-Exos group had a higher number of regenerated follicles than the control group (p < 0.001). In addition, we found that the skin tissues in the ADSC-Exos group had higher PDGF and vascular endothelial growth factor expressions and lower transforming growth factor beta 1 levels than those in the control group. Conclusion: Our results indicated that ADSC-Exos could promote in vivo hair follicle regeneration.

Stem Cell-Derived Exosomes: A New Method for Reversing Skin Aging

Wu Jinyan,Wu Sai-Nan,Zhang Li-Ping,Zhao Xiansheng,Li Yue,Yang Quyang,Yuan Ruoyue,Liu Jian-Lan,Mao Hong-Ju,Zhu Ningwen 한국조직공학과 재생의학회 2022 조직공학과 재생의학 Vol.19 No.5

Senescence is an inevitable natural life process that involves structural and functional degeneration of tissues and organs. Recently, the process of skin aging has attracted much attention. Determining a means to delay or even reverse skin aging has become a research hotspot in medical cosmetology and anti-aging. Dysfunction in the epidermis and fibroblasts and changes in the composition and content of the extracellular matrix are common pathophysiological manifestations of skin aging. Reactive oxygen species and matrix metalloproteinases play essential roles in this process. Stem cells are pluripotent cells that possess self-replication abilities and can differentiate into multiple functional cells under certain conditions. These cells also possess a strong ability to facilitate tissue repair and regeneration. Stem cell transplantation has the potential for application in anti-aging therapy. Increasing studies have demonstrated that stem cells perform functions through paracrine processes, particularly those involving exosomes. Exosomes are nano-vesicular substances secreted by stem cells that participate in cell-to-cell communication by transporting their contents into target cells. In this chapter, the biological characteristics of exosomes were reviewed, including their effects on extracellular matrix formation, epidermal cell function, fibroblast function and antioxidation. Exosomes derived from stem cells may provide a new means to reverse skin aging.

Soft High-Loading TiO2 Composite Biomaterial Film as an Efficient and Recyclable Catalyst for Removing Methylene Blue

Jinyan Hu,Dingsheng Wu,Quan Feng,Anfang Wei,Beibei Song 한국섬유공학회 2020 Fibers and polymers Vol.21 No.8

Currently, the preparation of novel efficient and recyclable photocatalysts for pollutant degradation in water hasbecome a research hotpot. In this study, a TiO2-loaded non-woven polypropylene/bacterial cellulose (TiO2-loaded NPBC)composite film was prepared by a biological culturing method. The TiO2 nanoparticles were spontaneously embedded intothe prepared composite film. Then, the composite film was used to degrade methylene blue (MB) and its degradation effectswere explored. The morphology of the composite film was investigated. It was clear that a large amount of TiO2nanoparticles were embedded into the composite membrane during the biological culturing method. The experimental resultsconfirmed that the TiO2-loaded NPBC composite film had good degradation performance and reusability. When the reactiontime was 120 min, the removal rate of MB by the film was 92.8 %. The removal rate remained above 85 % after 5degradation cycles.

The Structure and Evolution of Renewable Energy Trade Networks in the RCEP Region: Application of SNA Method

Jinyan Tian,Qianli Wu,손총영,LIUZIYANG 대외경제정책연구원 2024 East Asian Economic Review Vol.28 No.1

This paper utilizes social network analysis to examine the structural characteristics and trade dynamics of the renewable energy (hydropower, wind energy, and solar energy) trade network within the RCEP region from 2011 to 2020. The findings reveal: (1) The renewable energy trade network within the RCEP exhibits dynamism, heterogeneity, and an uneven development. The solar energy network is the most balanced and stable, while the wind energy network lags and shows marked fluctuations, with the hydropower network falling between these two. This demonstrates the diversity of energy trade within the region. (2) China, Singapore, and Japan are identified as the key exporting and importing countries, with Vietnam showing substantial growth potential. Individual analyses shed light on the stark disparities in trade status among nations, reflecting the diverse roles and future potential of member countries. (3) The QAP regression analysis reveals a significant influence of environmental pressure, particularly carbon dioxide emissions, on the renewable energy trade network. This study contributes to promoting environmental sustainability and energy security in the RCEP region and provides empirical evidence for global renewable energy trade strategies.

Evaluate the Gray Code in Distributed Fields for Tracking

Xianyun Wu,Kai Liu,Jinyan Cao,Yunsong Li,Li Wang 보안공학연구지원센터 2016 International Journal of Signal Processing, Image Vol.9 No.11

Distribution Fields (DFs) for tracking achieved a better performance than traditional algorithms due to its special representation that allows smoothing the objective function without destroying information about pixel values. DFs descriptor can satisfy both the specificity and smooth landscape requirements of a good tracking algorithm. In this paper, we evaluate the Gray code in original DFs algorithm by replacing the pixel values using Gray code instead of original binary code. Experimental results show that the Gray code can improve the tracking efficiency in certain way.

Effect of cement as mineral filler on the performance development of emulsified asphalt concrete

Liu, Baoju,Wu, Xiang,Shi, Jinyan,Wu, Xiaolong,Jiang, Junyi,Qin, Jiali Techno-Press 2020 Advances in concrete construction Vol.10 No.6

Cold-mixed asphalt mixture is a widely recommended asphalt pavement materials with potentially economic and environmental benefits. Due to the reduction of natural non-renewable mineral resources, powder minerals with similar properties are considered as new mineral fillers in asphalt mixtures. This study explored the feasibility of using cement to replace natural limestone powder (LP) in emulsified asphalt concrete modified by styrene-butadiene styrene copolymer. The experimental tests, including compressive strength, Marshall stability as well as moisture susceptibility test, were used to investigate the mechanical properties, the Marshall stability, flow value, as well as the moisture damage. In addition, the influence of material composition on the performance of asphalt concrete is explained by the microstructure evolution of the pore structure, the interface transition zone (ITZ), and the micromorphology. Due to mineralogical reactivity of cement, its replacement part of LP improved the mechanical properties, Marshall stability, but it will reduce the moisture susceptibility and flow value. This is because with the increase of the cement substitution rate, the pore structure of the asphalt concrete is refined, the width of ITZ becomes smaller, and the microstructure is more compact. In addition, asphalt concrete with a larger nominal particle size (AC-16) has relatively better performance.

Selection and identification of single-domain antibody against Peste des Petits Ruminants virus

Dan Liu,Lingxia Li,Xiaoan Cao,Jinyan Wu,Guoyu Du,Youjun Shang 대한수의학회 2021 Journal of Veterinary Science Vol.22 No.4

Background: Peste des petits ruminants (PPR) is an infectious disease caused by the peste des petits ruminants virus (PPRV) that mainly produces respiratory symptoms in affected animals, resulting in great losses in the world's agriculture industry every year. Single-domain variable heavy chain (VHH) antibody fragments, also referred to as nanobodies, have high expression yields and other advantages including ease of purification and high solubility. Objectives: The purpose of this study is to obtain a single-domain antibody with good reactivity and high specificity against PPRV. Methods: A VHH cDNA library was established by immunizing camels with PPRV vaccine, and the capacity and diversity of the library were examined. Four PPRV VHHs were selected, and the biological activity and antigen-binding capacity of the four VHHs were identified by western blot, indirect immunofluorescence, and enzyme-linked immunosorbent assay (ELISA) analyses. ELISA was used to identify whether the four VHHs were specific for PPRV, and VHH neutralization tests were carried out. ELISA and western blot analyses were used to identify which PPRV protein was targeted by VHH2. Results: The PPRV cDNA library was constructed successfully. The library capacity was greater than 2.0 × 106 cfu/mL, and the inserted fragment size was approximately 400 bp to 2000 bp. The average length of the cDNA library fragment was about 1000 bp, and the recombination rate was approximately 100%. Four single-domain antibody sequences were selected, and proteins expressed in the supernatant were obtained. The four VHHs were shown to have biological activity, close affinity to PPRV, and no cross-reaction with common sheep diseases. All four VHHs had neutralization activity, and VHH2 was specific to the PPRV M protein. Conclusions: The results of this preliminary research of PPRV VHHs showed that four screened VHH antibodies could be useful in future applications. This study provided new materials for inclusion in PPRV research.

Inhibition of caspase-1-dependent apoptosis suppresses peste des petits ruminants virus replication

Lingxia Li,Shengqing Li,Shengyi Han,Pengfei Li,Guoyu Du,Jinyan Wu,Xiaoan Cao,Youjun Shang 대한수의학회 2023 Journal of Veterinary Science Vol.24 No.5

Background: Peste des petits ruminants (PPR), caused by the PPR virus (PPRV), is an acute and fatal contagious disease that mainly infects goats, sheep, and other artiodactyls. Peripheral blood mononuclear cells (PBMCs) are considered the primary innate immune cells. Objectives: PBMCs derived from goats were infected with PPRV and analyzed to detect the relationship between PPRV replication and apoptosis or the inflammatory response. Methods: Quantitative real-time polymerase chain reaction was used to identify PPRV replication and cytokines expression. Flow cytometry was conducted to detect apoptosis and the differentiation of CD4+ and CD8+ T cells after PPRV infection. Results: PPRV stimulated the differentiation of CD4+ and CD8+ T cells. In addition, PPRV induced apoptosis in goat PBMCs. Furthermore, apoptosis and the inflammatory response induced by PPRV could be suppressed by Z-VAD-FMK and Z-YVAD-FMK, respectively. Moreover, the virus titer of PPRV was attenuated by inhibiting caspase-1-dependent apoptosis and inflammation. Conclusions: This study showed that apoptosis and the inflammatory response play an essential role in PPR viral replication in vitro, providing a new mechanism related to the cell host response.

Nicotine Induces the Expression of C-Reactive Protein via MAPK-Dependent Signal Pathway in U937 Macrophages

Junjun Mao,Juntian Liu,Xiaoming Pang,Ming Li,Jinyan Song,Chunjie Han,Di Wu,Shuyue Wang 한국분자세포생물학회 2012 Molecules and cells Vol.34 No.5

Atherosclerosis is an inflammatory disease in the vessel wall. Nicotine, a major component of cigarette smoke, is an independent risk factor for cardiovascular diseases including atherosclerosis. As an inflammatory molecule, C- reactive protein (CRP) participates in atherogenesis. Although it has been confirmed that CRP level in smoking patient is significantly higher than non-smokers and cigarette withdrawal,it is unknown whether nicotine induces CRP expression in macrophages. The present study was to observe effect of nicotine on CRP production and the related signal pathway in U937 macrophages. The results showed that nicotine significantly increased mRNA and protein expression of CRP in U937 macrophages in time- and concentration-dependent ways. Nicotinic acetylcholine receptor (nAChR) blocker hexamethonium, MEK1/2 inhibitor PD98059, p38 MAPK inhibitor SB203580 and NF-B inhibitor PDTC almost completely abolished nicotine-induced CRP expression in mRNA and protein levels in U937 macrophages. The further study indicated that hexa-methonium, PD98059, and SB203580 significantly inhibited ERK1/2 and p38 MAPK phosphorylation. These demonstrate that nicotine has ability to induce CRP ex-pression in macrophages through nAChR-ERK1/2/p38 MAPK-NF-B signal pathway, which contributes to better understanding of the pro-inflammatory and pro-atherosclerotic effects of nicotine in cigarette smokers.