Effects of puerarin on the Akt signaling pathway in bovine preadipocyte differentiation

Yun, Jinyan,Yu, Yongsheng,Zhou, Guoli,Luo, Xiaotong,Jin, Haiguo,Zhao, Yumin,Cao, Yang Asian Australasian Association of Animal Productio 2020 Animal Bioscience Vol.33 No.1

Objective: Puerarin has the potential of regulating the differentiation of preadipocytes, but its mechanism of action has not yet been elucidated. Adipocytes found in adipose tissue, the main endocrine organ, are the main sites of lipid deposition, and are widely used as a cell model in the study of in vitro fat deposition. This study aimed to investigate the effects of puerarin on adipogenesis in vitro. Methods: Puerarin was added to the culture medium during the process of adipogenesis. The proliferation and differentiation of bovine preadipocytes was measured through cell viability and staining with oil red O. The content of triacylglycerol was measured using a triglyceride assay kit. The mRNA and protein expression levels of adipogenic genes, peroxisome proliferator-activated receptor-γ (PPARγ) and CCAAT/enhancer-binding protein-α, were measured using quantitative real-time polymerase chain reaction and western blotting, respectively. Results: The addition of puerarin significantly increased adipogenesis of bovine preadipocytes and enhanced the mRNA and protein level expression of PPARγ (p<0.01). The expression of P-Akt increased after adipogenic hormonal induction, whereas puerarin significantly increased PPARγ expression by promoting the Akt signaling component, P-Akt. The mechanism of adipogenesis was found to be related to the phosphorylation level of Ser473, which may activate the downstream signaling of the Akt pathway. Conclusion: Puerarin was able to promote the differentiation of preadipocytes and improve fat deposition in cattle. The mechanism of adipogenesis was found to be related to the phosphorylation level of Ser473.

Comparative study on degradation of p-nitrophenol in aqueous solution by mFe/Cu/O3 and mFe0/O3 processes

Zhaokun Xiong,Jinyan Cao,Bo Lai,Ping Yang 한국공업화학회 2018 Journal of Industrial and Engineering Chemistry Vol.59 No.-

The performance of mFe/Cu/O3 and mFe0/O3 processes was comparatively investigated through optimized the key operational parameters for degradation of p-nitrophenol (PNP). The COD removal attained by mFe/Cu/O3 process was higher than that of mFe0/O3 process under the corresponding conditions. Additionally, under the optimal conditions, the COD removal (93.6%) obtained by mFe/Cu/O3 process was more than twice of the sum (44.6%) of that by ozone (35.3%) and mFe/Cu alone (9.3%). Finally, the key active materials in mFe/Cu/O3 system, degradation pathway of PNP and reaction mechanism of mFe/Cu/O3 process were proposed according to the analysis results of intermediate products, SEM–EDS and XRD.

Evaluate the Gray Code in Distributed Fields for Tracking

Xianyun Wu,Kai Liu,Jinyan Cao,Yunsong Li,Li Wang 보안공학연구지원센터 2016 International Journal of Signal Processing, Image Vol.9 No.11

Distribution Fields (DFs) for tracking achieved a better performance than traditional algorithms due to its special representation that allows smoothing the objective function without destroying information about pixel values. DFs descriptor can satisfy both the specificity and smooth landscape requirements of a good tracking algorithm. In this paper, we evaluate the Gray code in original DFs algorithm by replacing the pixel values using Gray code instead of original binary code. Experimental results show that the Gray code can improve the tracking efficiency in certain way.

Cell Growth Inhibition and Gene Expression Regulation by (-)-Epigallocatechin-3-Gallate in Human Cervical Cancer Cells

Yanyan Qiao,Xiaolin Shi,Jinyan Cao,Liangqun Xie 대한약학회 2009 Archives of Pharmacal Research Vol.32 No.9

EGCG [(-)-epigallocatechin-3-gallate] has shown its antitumor ability and perhaps a potential regimen for cancer patients. The goal of this study was to investigate the effect of EGCG on human papilloma virus (HPV) positive cervical cancer cell lines. EGCG inhibited the growth of CaSki (HPV16 positive) and HeLa (HPV18 positive) cells in a time- and concentration-dependent manner. Cell cycle arrest and apoptosis were observed in two cell lines after EGCG exposure. More importantly, we focused on EGCG regulation ability on pivotal genes involved in cervical cancer: viral oncogenes E6/E7, estrogen receptor (ER) and aromatase. Our results suggested that EGCG may be suitable for prevention and treatment of cervical cancer.

Selection and identification of single-domain antibody against Peste des Petits Ruminants virus

Dan Liu,Lingxia Li,Xiaoan Cao,Jinyan Wu,Guoyu Du,Youjun Shang 대한수의학회 2021 Journal of Veterinary Science Vol.22 No.4

Background: Peste des petits ruminants (PPR) is an infectious disease caused by the peste des petits ruminants virus (PPRV) that mainly produces respiratory symptoms in affected animals, resulting in great losses in the world's agriculture industry every year. Single-domain variable heavy chain (VHH) antibody fragments, also referred to as nanobodies, have high expression yields and other advantages including ease of purification and high solubility. Objectives: The purpose of this study is to obtain a single-domain antibody with good reactivity and high specificity against PPRV. Methods: A VHH cDNA library was established by immunizing camels with PPRV vaccine, and the capacity and diversity of the library were examined. Four PPRV VHHs were selected, and the biological activity and antigen-binding capacity of the four VHHs were identified by western blot, indirect immunofluorescence, and enzyme-linked immunosorbent assay (ELISA) analyses. ELISA was used to identify whether the four VHHs were specific for PPRV, and VHH neutralization tests were carried out. ELISA and western blot analyses were used to identify which PPRV protein was targeted by VHH2. Results: The PPRV cDNA library was constructed successfully. The library capacity was greater than 2.0 × 106 cfu/mL, and the inserted fragment size was approximately 400 bp to 2000 bp. The average length of the cDNA library fragment was about 1000 bp, and the recombination rate was approximately 100%. Four single-domain antibody sequences were selected, and proteins expressed in the supernatant were obtained. The four VHHs were shown to have biological activity, close affinity to PPRV, and no cross-reaction with common sheep diseases. All four VHHs had neutralization activity, and VHH2 was specific to the PPRV M protein. Conclusions: The results of this preliminary research of PPRV VHHs showed that four screened VHH antibodies could be useful in future applications. This study provided new materials for inclusion in PPRV research.

Inhibition of caspase-1-dependent apoptosis suppresses peste des petits ruminants virus replication

Lingxia Li,Shengqing Li,Shengyi Han,Pengfei Li,Guoyu Du,Jinyan Wu,Xiaoan Cao,Youjun Shang 대한수의학회 2023 Journal of Veterinary Science Vol.24 No.5

Background: Peste des petits ruminants (PPR), caused by the PPR virus (PPRV), is an acute and fatal contagious disease that mainly infects goats, sheep, and other artiodactyls. Peripheral blood mononuclear cells (PBMCs) are considered the primary innate immune cells. Objectives: PBMCs derived from goats were infected with PPRV and analyzed to detect the relationship between PPRV replication and apoptosis or the inflammatory response. Methods: Quantitative real-time polymerase chain reaction was used to identify PPRV replication and cytokines expression. Flow cytometry was conducted to detect apoptosis and the differentiation of CD4+ and CD8+ T cells after PPRV infection. Results: PPRV stimulated the differentiation of CD4+ and CD8+ T cells. In addition, PPRV induced apoptosis in goat PBMCs. Furthermore, apoptosis and the inflammatory response induced by PPRV could be suppressed by Z-VAD-FMK and Z-YVAD-FMK, respectively. Moreover, the virus titer of PPRV was attenuated by inhibiting caspase-1-dependent apoptosis and inflammation. Conclusions: This study showed that apoptosis and the inflammatory response play an essential role in PPR viral replication in vitro, providing a new mechanism related to the cell host response.