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Xinpeng Zhang,Jinshan Jia,Ming-yuan Zhao,Cheng Li,Xu Han,Zongda Xu 한국원예학회 2022 Horticulture, Environment, and Biotechnology Vol.63 No.1
Paeonia suffruticosa ‘Shima Nishiki’ is a valuable double-color cultivar, but the mechanism of its double-color formationis still unknown at the post-translational level. Moreover, many studies have shown that microRNAs (miRNAs) play a keyrole in regulating anthocyanin biosynthesis. In this study, small RNA sequencing was performed to identify and characterizemiRNAs in six libraries of the red (LH) and pink (LF) petals. A total of 591 conserved and 53 novel miRNAs wereidentified, among which 68 conserved and 2 novel miRNAs were differentially expressed between the LH and LF libraries. Meanwhile, the corresponding target genes for these differentially expressed miRNAs (DEMs) were successfully predictedand annotated. Among them, two DEMs (miR858 and miR156a-5p) and their corresponding target genes possibly relatedto anthocyanin biosynthesis were screened. We further confirmed the relative expression of 15 candidate miRNAs (2 DEMsand other 13 miRNAs) and the 4 corresponding target genes of these DEMs by quantitative real-time PCR (qRT-PCR). Among these four target genes, only CL2846.Contig2_All [Annotation: transcription factor MYB12] showed a substantiallyhigher differential expression between the red and pink petals. Based on the above results, we concluded that miR858 andmiR156a-5p (especially miR858) are possibly involved in mediating double-color formation. These results provide a newunderstanding about the mechanism of double-color formation in P. suffruticosa ‘Shima Nishiki’.
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Cordyceps militaris Extract Attenuates D-Galactose-Induced Memory Impairment in Mice
Zaixin Li,Zhi Zhang,Jinshan Zhang,Jing Jia,Jie Ding,Rongzhen Luo,Zhangqin Liu 한국식품영양과학회 2012 Journal of medicinal food Vol.15 No.12
Memory impairment is one of main clinical symptoms of brain senescence. To address the effects of Cordyceps militaris Link extract (CE) on memory impairment, a D-galactose (D-Gal)–induced aging mouse model was employed. Mice injected with D-Gal showed a significant learning and memory impairment that was rescued by CE treatment. The mechanism was further investigated by analyzing the protein level and activity of oxidant and antioxidant molecules, including malondialdehyde (MDA), monoamine oxidase (MAO), total super-oxide dismutase (T-SOD), total antioxidant capacity (T-AOC), glutathione (GSH), and glutathione peroxidase (GSH-px), which played critical roles in the development of brain senescence. The results showed that CE treatment resulted in a significant decrease in the oxidative activity of MAO and the level of MDA, and significantly increased the antioxidant activities of T-SOD and T-AOC in the cerebral cortices. Moreover, the level of GSH and the activity of antioxidant enzymes GSH-px in serum were significantly upregulated after CE treatment. Taken together, our results suggest that Cordyceps militaris extract could ameliorate experimental memory impairment in mice with D-Gal-induced aging through its potent antioxidant activities.
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Yan, XiangMin,Wang, Jia,Li, Hongbo,Gao, Liang,Geng, Juan,Ma, Zhen,Liu, Jianming,Zhang, Jinshan,Xie, Penggui,Chen, Lei Asian Australasian Association of Animal Productio 2021 Animal Bioscience Vol.34 No.9
Objective: With the rapid development of proteomics sequencing and RNA sequencing technology, multi-omics analysis has become a current research hotspot. Our previous study indicated that Xinjiang brown cattle have better meat quality than Kazakh cattle. In this study, Xinjiang brown cattle and Kazakh cattle were used as the research objects. Methods: Proteome sequencing and RNA sequencing technology were used to analyze the proteome and transcriptome of the longissimus dorsi muscle of the two breeds of adult steers (n = 3). Results: In this project, 22,677 transcripts and 1,874 proteins were identified through quantitative analysis of the transcriptome and proteome. By comparing the identified transcriptome and proteome, we found that 1,737 genes were identified at both the transcriptome and proteome levels. The results of the study revealed 12 differentially expressed genes and proteins: troponin I1, crystallin alpha B, cysteine, and glycine rich protein 3, phosphotriesterase-related, myosin-binding protein H, glutathione s-transferase mu 3, myosin light chain 3, nidogen 2, dihydropyrimidinase like 2, glutamate-oxaloacetic transaminase 1, receptor accessory protein 5, and aspartoacylase. We performed functional enrichment of these differentially expressed genes and proteins. The Kyoto encyclopedia of genes and genomes results showed that these differentially expressed genes and proteins are enriched in the fatty acid degradation and histidine metabolism signaling pathways. We performed parallel reaction monitoring (PRM) verification of the differentially expressed proteins, and the PRM results were consistent with the sequencing results. Conclusion: Our study provided and identified the differentially expressed genes and proteins. In addition, identifying functional genes and proteins with important breeding value will provide genetic resources and technical support for the breeding and industrialization of new genetically modified beef cattle breeds.
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