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Xinpeng Zhang,Jinshan Jia,Ming-yuan Zhao,Cheng Li,Xu Han,Zongda Xu 한국원예학회 2022 Horticulture, Environment, and Biotechnology Vol.63 No.1
Paeonia suffruticosa ‘Shima Nishiki’ is a valuable double-color cultivar, but the mechanism of its double-color formationis still unknown at the post-translational level. Moreover, many studies have shown that microRNAs (miRNAs) play a keyrole in regulating anthocyanin biosynthesis. In this study, small RNA sequencing was performed to identify and characterizemiRNAs in six libraries of the red (LH) and pink (LF) petals. A total of 591 conserved and 53 novel miRNAs wereidentified, among which 68 conserved and 2 novel miRNAs were differentially expressed between the LH and LF libraries. Meanwhile, the corresponding target genes for these differentially expressed miRNAs (DEMs) were successfully predictedand annotated. Among them, two DEMs (miR858 and miR156a-5p) and their corresponding target genes possibly relatedto anthocyanin biosynthesis were screened. We further confirmed the relative expression of 15 candidate miRNAs (2 DEMsand other 13 miRNAs) and the 4 corresponding target genes of these DEMs by quantitative real-time PCR (qRT-PCR). Among these four target genes, only CL2846.Contig2_All [Annotation: transcription factor MYB12] showed a substantiallyhigher differential expression between the red and pink petals. Based on the above results, we concluded that miR858 andmiR156a-5p (especially miR858) are possibly involved in mediating double-color formation. These results provide a newunderstanding about the mechanism of double-color formation in P. suffruticosa ‘Shima Nishiki’.
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