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吳春花 한국중국문화학회 2001 中國學論叢 Vol.11 No.-
隨着中國政治經濟文化科技等領域的發展, 加入世界貿易組織的國際形式下, 社會需要的是複合型外語人才, 幷需要大批具有高水平的專業飜譯人才. 單一外語專業知識和基礎技能型人才已現現出對市場的不適應. 如何培養複合型外語` 飜譯人才已是外語敎學的大課題. 本論文從飜譯學角度論述飜譯的槪念, 以及飜譯類型, 還從韓國飜譯大學院的科程與筆者多年的現場敎學經驗來硏究` 摸索培養符合21世紀社會需要的飜譯人才的敎學目標爲基礎的有效飜譯敎學方案. 通過本論文的發表, 希望中韓學術界對中國韓國語敎育中的飜譯敎學有更深的了解, 幷能對飜譯敎學質量的提高作出微薄貢獻, 筆者將感到幕大欣慰.
Effect of fenpropathrin on the viability and homing ability of worker bees Apis mellifera
Chun-hua Liao,Jie Wu,Zi-long Wang,Zhi Jiang Zeng,Xiaobo Wu 한국응용곤충학회 2017 Journal of Asia-Pacific Entomology Vol.20 No.4
To explore the effect of fenpropathrin on survival and homing ability of honeybees Apis mellifera L., the newly emerged honeybee workers (< 12 h old) were randomly divided into 4 groups with 3 replicates in each group. Fenpropathrin (1/2 LD50, 1/4 LD50, 1/8 LD50 and 0% LD50) was added on the thorax of the bees. The viability of worker bees and their homing rate at 1 km distance away from colonies were analyzed, and the expression levels of two memory related genes (GluRA and Nmdar 1) in 20-day–old worker bees were also quantified. Overall, the lifespan and homing rates were significantly decreased with the increase of fenpropathrin dose (P < 0.05), but there was no significant difference between the least group (1/8 LD50) and the control group (0% LD50) (P > 0.05). The relative expression of Nmdar1 was decreased significantly with the increasing doses of fenpropathrin and the lower expression level of Nmdar 1 was found in the fenpropathrin-treaded groups. The expression level of GluRA of workers in 1/8 LD50 group and the control group were significantly higher than that in 1/2 LD50 group and 1/4 LD50 group (P < 0.05), whereas the expression level of GluRA of workers in 1/4 LD50 group was significantly higher than that in 1/2 LD50 group (P < 0.05), and there is no significant difference between 1/8 LD50 group and the control group (P > 0.05). In conclusion, the use of fenpropathrin for agricultural crops may show negative influence on the viability and homing ability of worker bees Apis mellifera L.
Chun-Hua Wu,Eun-Joo Hahn,Kee-Yoeup Paek 한국작물학회 2007 한국작물학회 학술발표대회 논문집 Vol.2007 No.11
Echinacea purpurea is an important medicinal plant native of North America, which contains caffeic acid derivatives, alkamides, glycoproteins, and polysaccharides. For commercial roduction of bioactive compounds from Echinacea purpurea, adventitious roots were induced and proliferated in 5 and 20-liter bioreactors under various cultural conditions (Medium composition, inoculum density, air volume, light, temperature et al.). In a 5-liter bioreactor, more than 10-fold of biomass increment was achieved after 5 weeks of culture. Increases of root biomass and bioactive compounds were correlated with increased consumption of sucrose and macro/micro nutrients in the medium. Elicitations have been conducted with UV-C, NO, and SA to increase the accumulation of total polyphenolics, flavonoids and caffeic acid derivatives in the adventitious root cultures, which resulted in 1.2-1.6 times of increment. Based on the previous experimental results, 500-liter and 1000-liter scale bioreactors were applied for large scale cultures of Echinacea purpurea adventitious roots. Total root biomass of 3.62 kg and 5.05kg (dry weight) were achieved in a 500 L and a 1000 L bioreactor, respectively. The accumulation of 22.55 mg/g DW cichoric acid, 4.92 mg/g DW chlorogenic acid and 3.99 mg/g DW caftaric acids were also achieved from the adventitious root in a 1000-liter scale bioreactor.
Wu, Chun-Hua,Murthy, Hosakatte Niranjana,Hahn, Eun-Joo,Paek, Kee-Yoeup 대한약학회 2007 Archives of Pharmacal Research Vol.30 No.8
The aim of this study was to produce caffeic acid derivatives from adventitious root cultures of Echinacea purpurea, which are of high pharmaceutical value. The effects of both media optimization and replenishment strategies were adopted to achieve improved production of E. purpurea adventitious roots and caffeic acid derivatives. Of the different media strengths (0.25 MS, 0.5 MS, 0.75 MS and 1 MS) tested for the culturing of adventitious roots in 5 L bioreactors, 0.5 MS medium was found to be most suitable for the growth of adventitious roots. The optima accumulation of biomass (73.6 g $L^{-1}$ FW and 10.03 g $L^{-1}$ DW), phenolics (61.14 mg $g^{-1}$ DW) and flavonoids (38.30 mg $g^{-1}$ DW) were achieved in this medium. Furthermore, fed batch cultivations (media replenishment with 0.25 MS, 0.5 MS, 0.75 MS and 1 MS at the end of $2^{nd}$ and $3^{rd}$ weeks) to further enhance the production of adventitious root biomass and metabolites were also attempted. High adventitious root biomasses (83.1 g $L^{-1}$ FW and 15.30 g $L^{-1}$ DW) were achieved with feeding of the 0.5 MS medium at the end of $2^{nd}$ week. This led to slight decreases in the total production of phenolics and flavonoids; however, this feeding was responsible for increases in the accumulation of caftaric acid (5.76 mg $g^{-1}$ DW) and cichoric acid (26.12 mg $g^{-1}$ DW).
Chun-Hua Wu,Hosakatte Niranjana Murthy,Eun-Joo Hahn,백기엽 대한약학회 2007 Archives of Pharmacal Research Vol.30 No.8
The aim of this study was to produce caffeic acid derivatives from adventitious root cultures of Echinacea purpurea, which are of high pharmaceutical value. The effects of both media optimization and replenishment strategies were adopted to achieve improved production of E. purpurea adventitious roots and caffeic acid derivatives. Of the different media strengths (0.25 MS, 0.5 MS, 0.75 MS and 1 MS) tested for the culturing of adventitious roots in 5 L bioreactors, 0.5 MS medium was found to be most suitable for the growth of adventitious roots. The optima accumulation of biomass (73.6 g L-1 FW and 10.03 g L-1 DW), phenolics (61.14 mg g-1 DW) and flavonoids (38.30 mg g-1 DW) were achieved in this medium. Furthermore, fed batch cultivations (media replenishment with 0.25 MS, 0.5 MS, 0.75 MS and 1 MS at the end of 2nd and 3rd weeks) to further enhance the production of adventitious root biomass and metabolites were also attempted. High adventitious root biomasses (83.1 g L-1 FW and 15.30 g L-1 DW) were achieved with feeding of the 0.5 MS medium at the end of 2nd week. This led to slight decreases in the total production of phenolics and flavonoids; however, this feeding was responsible for increases in the accumulation of caftaric acid (5.76 mg g-1 DW) and cichoric acid (26.12 mg g-1 DW).