사춘기 이전의 아동에 있어서 야간 수면 중의 성장 호르몬, 코티졸 분비의 남녀 차이에 관한 연구

김진관,신철,이창규,이승관 高麗大學校 倂設 保健大學 保健科學硏究所 2001 保健科學論集 Vol.27 No.1

Growth hormone is secreted episodically from the pituitary gland in relation to the onset of SWS, with maximal secretion occurring in the first half of the night, whereas cortisol secretion inhibited by SWS in the first half of night, increased in response to activation of hypothalamic-pituitary-adrenal axis tone in the second half of the night. But numerous studies have delineated association between sleep parameter and neuroendocrine function in the adult, few investigation have study in prepubertal children. As the present study, we investigated the relationship between GH, Cortisol secretion and sleep parameter during nocturnal sleep in prepubertal childrens. 19 childrenstmale 10, female 9) were recruited to study from community advertisement, performend polysomnography in sleep laboratory and serially collected 30 min interval blood sampling for hormone measurement from pm 10:00 to am 6:30. Sex difference were not significantly in total secretion of GH, Cortisol level, but GH secretion was sexually dimorphic with a more pulsatile release in female than male childrens during the second half of night. The first half of night SWS(%) was significantly correlated with integrated GH levels from 22:00 to 23:30, 24:00(r=0.532 p<0.05, r=0.55 p<0.05), the REM sleep(%) during night was significantly correlated with integrated cortisol levels (r=0.830,p<0.001), this results represent that SWS may be a partial stimulating factor of GH secretion, REM sleep may be a activation of HPA axis in prepubertal children

성인 남성에 있어서 수면 무호흡 증후군에 예측인자에 관한 연구

김진관,신철,이승관,이창규 高麗大學校 倂設 保健大學 保健科學硏究所 2001 保健科學論集 Vol.27 No.2

Obstructive Sleep Apnea Syndromes (OSAS) is charicterized by episodes of complete or partial pharyngeal obstruction during sleep. Epidemiological studies estimate that 2 to 5% of the population meet the minimal diagnostic criteria, and some community based studies have found that about 2% of women and 4% of men between 30 and 60 years old are affected by OSAS. We studied 150male OSAS patient during a period of one year and recorded age and anthropomorphic data. We performed polysomnography. Several polysomngrophic data were compard between OSAS and control group. There were differences in sleep indices such as sleep stage 1, stage REM LDP, snoring, Aarousal index and RDI between two group. RDI and LDP correlated positively with BMI WHR chest circumference and Neck circumference. It can be concluded that obesity is a predisposing risk factor of OSAS.

Black Rice (Oryza sativa L. var. japonica) Hydrolyzed Peptides Induce Expression of Hyaluronan Synthase 2 Gene in HaCaT Keratinocytes

( Gwan Sub Sim ),( Dong Hwan Lee ),( Jin Hwa Kim ),( Sung Kwan An ),( Tae Boo Choe ),( Tae Jong Kwon ),( Hyeong Bae Pyo ),( Bum Chun Lee ) 한국미생물생명공학회 2007 Journal of microbiology and biotechnology Vol.17 No.2

Phenolic Extractives from Ligularia ficheri Leaves

Jin Kyu Kim,Seong Su Hong,Jin Gwan Kwan,Changon Seo,Wonsik Jeong,Yun-Hyeok Choi,Chunwhan Choi,Joa Sub Oh 한국약용작물학회 2013 한국약용작물학술대회 발표집 Vol.2013 No.05

Phenolic Extractives from Ligularia ficheri Leaves

Jin Kyu Kim(김진규),Seong Su Hong(홍성수),Jin Gwan Kwan(권진관),Wonsik Jeong(정원식),Yun-Hyeok Choi(최윤혁),Changon Seo(서찬곤),Chunwhan Choi(최춘환),Joa Sub Oh(오좌섭) 한국약용작물학회 2013 한국약용작물학회 학술대회논문집 Vol.2013 No.1

성인 남성에 있어서 수면 무호흡 증후군의 예측인자에 관한 연구

김진관(Jin-Kwan Kim),신철(Chol Shin),이승관(Seung-Gwan Lee),이창규(Chang-Kyou Lee) 고려대학교 보건과학연구소 2001 보건과학논집 Vol.27 No.2

Shattering-related Characteristics and Germination Speed Based on Grain Type and Esterase Isozyme Zymogram in Korean Off-rype Rice

Dong Kwan Kim,Il Doo Jin,Yeon Gyu Kim,Kyung Soo Min,Byung Gwan Jung,Yong Jae Kim 韓國作物學會 2002 Korean journal of crop science Vol.47 No.1

This study was carried out to investigate the characteristics of shattering habit and germination of offtype rice plants collected in Korea which were classified into seven groups based on grain characteristics. In the long-grain red rice group, the short-grain red rice group, the long-grain normal rice group, and the long-grain waxy rice group, the tensile strength of grain and primary rachis branch was relatively low and the fiber cell of the primary rachis branch was short. Characteristics of shattering-related traits and germination in relation to grain and esterase isozyme zymogram type of off-type rice plants. In most lines of the extremely late and sterile rice group, the tensile strength of grain was low, while that of primary rachis branch was high, and the fiber cell was long. However, in the type 1 esterase isozyme zymogram(EIZ) lines among the long-grain normal rice group and the type 6 and 5+6 EIZ plants among the extremely late and sterile rice group, the tensile strength of grain was relatively higher. The long-grain waxy rice group and the type 3 EIZ lines among the long-grain red rice group, showed higher germinability than did cultivars which are known to germinate well even in low-temperature. The other off-type rice group, except for the short-grain waxy rice group and type 1 and 7 EIZ lines among the long-grain normal rice group, had higher germination speed than that of the cultivars.

폐쇄성 수면 무호홉증에서의 Homocysteine Level과 MTHFR 유전자 다형성

김진관 ( Jin Kwan Kim ),신철 ( Chol Shin ),이창규 ( Chang Kyu Lee ),이승관 ( Seung Gwan Lee ) 대한임상검사과학회 2002 대한임상검사과학회지(KJCLS) Vol.34 No.2

배경 : 폐쇄성 수면 무호흡은 독립적으로 고혈압, 심혈관 질환, 치매의 위험요인으로 알려져 있다. 이러한 연관성에 대한 정확한 기전은 아직 밝혀져 있지 않지만, 야간의 hypoxicsπess로 인한 혈관손상이 이러한 질병을 유발하는 것으로 최근 보고되고 있다. 이에 본 연자는 폐쇄성 수면 무호흡 환자에서 심혈관계 질환의 위험요인으로 알려진 homocystein 농도와의 연관성에 대하여 알아보고자 본 연구를 시행하였다. 방법 Community-based ansan study 가운데, 야간의 portable 수면검사를 실시한 총81명의 중년남성을 대상으로 시행하였으며, 이 가운데 RDI ≥ 20 (moderate to severe OSA, group A),5 RDI<20 (mild OSA, group B), RDI<5 (control, group C)로 정의하였다. 연구에 포함된 대상군은 설문지를 이용하여 과거력 및 수면 위생에 대하여 조사하였고, 혈중 homocysteine,vitamin B12, folic acid 농도를 측정하였으며, DNA 추출 후 MTHFR 의 유전자 다형성에 대하여 조사하였다. 결 과 : 세 군간의 biochemical data 및 homocysteine 의 평 균비 교에 있어서 , biochechemicaldata는 유의한 차이가 없었지만, homocysteine 농도는 세 군간에 유의한 차이가 있었다. (Gr. A:14.72.7 Gr. B : 9.74.4 Gr.C : 7.73.7, p<0.001). 그러나 수면 무호흡군에 있어서 MTHFR 유전자 다형성 (C677T)의 유의한 차이를 보이지 않았다. (p<0.175). 또한 homocysteine 농도에 대한multiple regression analysis 분석 결과, RDI, MTHFR 유전자의 동형 접합성 유전자 변이(T677T)가 유의한 연관성이 있는 것으로 분석되었다. 결론 : 야간의 심한 수면 무호흡은 독립적으로 homocysteine 농도에 영향을 줄수 있으며, 이러한 결과는 수면 무호흡과 섬혈관계 질환의 연관성에 대한 부분적인 근거를 마련할수 있을 것으로 생각되며, 원인에 대한 추가적인 연구가 필요할 것으로 사료된다. Obstructive sleep apnea (OSA) is characterized by repetitive obstruction of upper airway, leading to repeated arterial oxygen desaturation. The prevalence of cardiovascular disease, including arterial hypertension, myocardial infarction, atherosclerosis and stroke is increased among patients with OSA. Although exact mechanism for this reason is not understood, it is postulated that hypoxic stress induced by OSA may be involved in the etiology of cardiovascular disorder. Homocysteine (Hey), which has been reported as independent risk factors for vascular disease in many studies, is a sulfur-containing amino acid formed during the metabolism of methionine. Therefore, we hypothesized that OSA induced hypoxic stress might be correlated with oxidative stress, we investigated association between homocysteine and MTHFR gene mutation in OSA patients. Levels of homocysteine, cholesterol, low-density lipoprotein, high-density lipoprotein, try glycerides, creatinine, vitamin B 12 and folic acid were determined in 82 participants after overnight fasting. All subjects underwent computerized portable polysomnogram (PSG) in the home. Among these study participants, inclusion criteria were Apnea Hypopnea Index (AHI)>20 for moderate to severe OSA (Group A), 5 ~AHI ~20 for mild OSA subjects (Group B) and AHI<5 for control subjects (Group C) matched by age. Moderate to severe OSA patients has significantly higher homocysteine levels (14.72.7 umolfL, P<0.001) than other groups. MTHFR (C677T) gene polymorphism did not differ between patients and control. Homocysteine level showed positive correlation with square root transformed AHI (r=0.663, P<0.001). Also, AHI and MTHFR gene mutation (T677T) have an independent effect on homocysteine levels in multiple regression models. Severe OSA is an independent effect on elevated homocysteine levels. We hypothesize that these results may be explained by endothelial dysfunction combined with excess free-radical formation in OSA patients. Further study will be warranted to elucidate the relationship between hypoxic stress and homocysteine levels in OSA patients.

인공 강우 시뮬레이터의 강우 특성 분석을 통한 최적 운영방법 제안

김진관 ( Jin Kwan Kim ),강재원 ( Jae Won Kang ),정관수 ( Gwan Soo Jeong ),최훈 ( Hoon Choi ),김민석 ( Min Seok Kim ) 한국지리학회 2016 한국지리학회지 Vol.5 No.1

산사태, 토사유출 실내실험을 위한 인공강우기의 특성과 이의 운영은 매우 중요하다. 본 고에서는 새로 고안된 인공강우기의 강우에너지 특성분석을 통해 인공강우기의 최적 운영 방법에 대해 연구하였다. 이를 위하여 세 개의 사례(Case)로 구분하여 사례별 강우강도와 강우입자의 에너지 분포를 측정하였다. Case 1은 각각의 노즐 조합별로 입력유량 차이에 따른 강우강도 분포를 측정하였으며, Case 2는 동일조건에서 노즐조합 차이에 따른 강우강도 분포를 측정하였고, 마지막 Case 3은 노즐직경 차이에 따른 강우강도 분포를 측정하였다. 강우강도의 공간적 분포를 고려하여 2가지 최적 강우운영조건, 즉 Condition 1과 Condition 2를 각각 제시하였다. 본 연구에서의 인공강우 시뮬레이터는 자연강우에 비하여 직경이 큰 강우입자의 부재로 인하여 자연상태의 강우에 비하여 강우에너지가 크게 적은 것으로 나타났다. 하지만, 기존의 연구에서 사용된 중소 규모의 여러 인공강우 시뮬레이터의 특성과 비교한 결과 큰 차이가 없는 것으로 밝혀졌다. 앞으로 보다 더 정확한 실험을 위해서는 자연강우특성에 가까운 인공강우기 제작이 요구된다. The characteristics of rainfall simulator are so important for the laboratory experiment of landslide and sediment runoff. To analyze the characteristics of the newly designed rainfall simulator and decide its optimum operating methods, we measured the rainfall intensity and the kinetic energy of raindrops with three cases; Case I: different nozzle combinations and different input currents, Case II: only different nozzle combinations, and Case III: only different nozzle sizes. On the base of the measuring results, we suggested two optimum conditions (condition 1 and 2) for the artificial rain simulation. Although there was large difference between the characteristics of natural rainfall and those of simulated rainfall, the kinetic energy distribution of newly designed rainfall simulator was similar to those of many previous studies. However, the new design of rainfall simulator having the characteristics of natural rainfall will be needed for further detailed laboratory study reflecting the natural conditions.

발아 검은쌀 올리고펩타이드의 각질형성세포에서 Hyaluronan Synthase 발현과 피부 탄력 개선 효과

심관섭 ( Gwan Sub Sim ),이동환 ( Dong Hwan Lee ),김진화 ( Jin Hwa Kim ),이범천 ( Bum Chun Lee ),안성관 ( Sung Kwan Ahn ),최태부 ( Tae Boo Choe ),표형배 ( Hyeong Bae Pyo ) 대한화장품학회 2006 대한화장품학회지 Vol.32 No.1

본 연구에서는 발아 검은 쌀로부터 분리한 올리고펩타이드($Oligosproutin^®$)의 피부 탄력 개선 효과에 대하여 연구하였다. 올리고펩타이드가 각질형성세포의 유전자 발현에 미치는 영향을 알아보고자 DNA microarray를 수행하였다. 각질형성세포에 올리고펩타이드를 처리하여 유전자의 발현이 2배 이상 증가하는 유전자는 745개이고, 2배 이상 감소하는 유전자는 1011개로 나타났다. 이 중 hyaluronan synthase 2 (HAS2)의 유전자 발현이 2배 이상 증가함을 확인하였다. 올리고펩타이드를 처리한 각질형성세포에서 세포활성 증가, HAS2 유전자 발현 증가, 세포내 항산화 효과를 확인하였다. 올리고펩타이드를 함유한 O/W 에멀젼을 이용한 임상실험에서 피부의 탄력 개선 효과를 확인하였다. 본 연구를 통하여 발아 검은 쌀로부터 분리한 올리고펩타이드는 피부 탄력 개선을 주는 항노화 화장품 개발에 응용될 수 있을 것이라 기대된다. In this study, the effect of oligopeptide ($Oligosproutin^®$) from sprouted black rice was evaluated for possible improvement in skin elasticity. We examined the changes in gene expression on oligopeptide-treated HaCaT cells using DNA microarray analysis. As a result, oligopeptide treatment showed a differential expression ratio of more than 2-fold : 745 genes were activated and 1011 genes were repressed. One of the most interesting findings is a 2-fold increase in hyaluronan synthase 2 (HAS 2) gene expression by oligopeptide. We also found that oligopeptide increased cell proliferation, HAS2 mRNA expression and intracellular ROS scavenging activity in HaCaT cells. A human clinical study which oil-in-water emulsion with oligopeptide was topically applied showed significant increase in skin elasticity. These results suggest that the sprouted black rice oligopeptide ($Oligosproutin^®$) can be effective anti-aging ingredient for cosmetics.