Enhancement on antioxidant and antibacterial activities of Brightwell blueberry by extraction and purification

Liu Haonan,Wu Han,Wang Ying,Wang Fan,Liu Xiaoli,Zhou Jianzhong 한국응용생명화학회 2021 Applied Biological Chemistry (Appl Biol Chem) Vol.64 No.6

A blueberry anthocyanin extract was obtained from Brightwell blueberry fruits cultivated in eastern China and the extraction and purification conditions were optimized. The components of the anthocyanin extract were identified using ultra-performance liquid chromatography-electrospray ionization interface-mass spectrometer. The antioxidant and antibacterial activities of the blueberry fruit supernatant (BFS), blueberry anthocyanin crude extract (BCE), and blueberry anthocyanin rich extract (BRE) were evaluated. The extraction yield was 1.79 ± 0.0014 mg/g under the following optimal conditions: 1:20 solid-to-liquid ratio (v/w), 24 h, 34 °C, and 90% ethanol containing 0.21% (v/v) hydrochloric acid. With regard to purification, anthocyanin purity increased 19.1-fold. Nine fractions were identified as the glycosides of delphinidin, cyanidin, petunidin, and malvidin. The biological activities of the blueberry anthocyanin extract were improved through extraction and purification. Compared with BFS and BCE, BRE had a higher DPPH radical scavenging activity ( EC50 = 0.51 mg/mL), ABTS antioxidant capacity ( EC50 = 0.32 mg/mL), and oxygen radical absorbance capacity (0.43 mmol Trolox/g). Furthermore, BRE (2 mg/mL) showed a maximum of 84.64 ± 0.35% reduction in the biofilm biomass of Listeria monocytogenes and the inhibition zone given by BRE against Escherichia coli was 16.04 ± 0.38 mm. BRE showed the highest antioxidant capacities and obvious antibacterial effects against foodrelated microorganisms than the other samples. Therefore, BRE can be used as a natural antioxidant and antibacterial agent and has potential health advantages and food industry applications.

Glucocorticoid-induced expansion of classical monocytes contributes to bone loss

Liu Pei,Gao Youshui,Luo Pengbo,Yu Hongping,Guo Shang,Liu Fuyun,Gao Junjie,Xu Jianzhong,Wang Shengdian,Zhang Changqing 생화학분자생물학회 2022 Experimental and molecular medicine Vol.54 No.-

Classical monocytes are commonly involved in the innate inflammatory response and are the progenitors of osteoclasts. Excess endogenous glucocorticoids (GCs) can increase the levels of classical monocytes in blood and bone marrow. The role of this cell population in high-dose exogenous GC-induced osteoporosis (GIOP) remains to be elucidated. In this study, GIOP was established in rats and mice by daily methylprednisolone injection, and monocyte subsets were analyzed by flow cytometry. We demonstrated that classical monocytes accumulate in bone marrow during GIOP. Similarly, the monocyte proportion among bone marrow nucleated cells was also increased in patients with steroid treatment history. We sorted classical monocytes and analyzed their transcriptional profile in response to GCs by RNA sequencing. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis showed that classical monocytes isolated from GC-treated rats exhibited osteoclast differentiation potential. Deletion of classical monocytes by clodronate liposome treatment prevented GIOP via inhibition of osteoclastogenesis and restoration of CD31HiendomucinHi vessels. Regarding the molecular mechanism, classical monocytes express high levels of glucocorticoid receptors. In vitro treatment with GCs increased both the percentage and absolute number of monocytes and promoted their proliferation. In summary, classical monocytes mediated GC-induced bone loss and are a potential target for therapeutic intervention in GIOP treatment.

Study on the Reduction Properties of Thiourea Dioxide and Its Application in Discharge Printing of Polyester Fabrics

Xiaoyun Liu,Mengmeng Xie,Yanchao Li,Lan Zhou,Jianzhong Shao 한국섬유공학회 2018 Fibers and polymers Vol.19 No.6

The thiourea dioxide (TDO) was tentatively applied as a reductive discharging agent onto the discharge printing of polyester fabrics. The effect of aqueous solution temperature on the reducing capacity of TDO was thoroughly investigated by measuring oxidation-reduction potentials and hydrolysis kinetic curves. The effects of TDO on disperse dyes in aqueous solution and disperse dyes printed on the surfaces of glass substrates or polyester fabrics were investigated by analyzing the color changes before and after TDO discharge treatment. It was confirmed that TDO reduction became stronger with increasing aqueous solution temperature. TDO could destroy the chromogenic groups of the selected azo-dyes in aqueous solution and on the surfaces of glass substrates. Different from cotton and silk fabrics, the discharge effect of TDO directly depended on the compact structure of the polyester fibers. When a discharge accelerant was added into white discharge pastes with TDO, the compact structure of polyester fibers was opened up to achieve a good discharge effect. This study provides a new method for TDO development and a new strategy for the discharge printing of polyester fabrics.

Particle trajectory and orientation evolution of ellipsoidal particles in bounded shear flow of Giesekus fluids

Bingrui Liu,Jianzhong Lin,Xiaoke Ku,Zhaosheng Yu 한국유변학회 2021 Korea-Australia rheology journal Vol.33 No.4

The migration of ellipsoidal particles in bounded shear flow of Giesekus fluids is studied numerically using the direct forcing/fictitious domain method for the Weissenberg number ranging from 0.1 to 3.0, the mobility parameter α which quantifies the shear-thinning effect ranging from 0.1 to 0.7. The model and numerical method are validated by comparing the present results with available theoretical and numerical results in other literatures. The results show that the trajectory of particles depends on their initial orientation and vertical position, and the particle migration can be roughly classified into returning and passing pattern. The values of initial vertical position of particle corresponding to the separatrix between the returning and passing pattern decrease with increasing Weissenberg number regardless of the initial orientation of particle, and the shear thinning has the opposite effect. The evolution of particle orientation depends on the initial particle orientation. For the particles whose initial orientation is parallel to the shear plane, the particle rotates with the semi-major axis as radius in the shear plane. For the particles whose initial orientation is perpendicular to the shear plane, the particle rotates with the semi-minor axis as radius. For the particles whose initial orientation has a certain angle with the shear plane, the particle rotates with the vorticity axis and the orientation vector is gradually close to the vorticity vector. The evolution of the particle orientation becomes slow with increasing Wi whether it is in passing behavior or in returning behavior.

Efficient Expression and Purification of Recombinant Alcohol Oxidase in Pichia pastoris

Yunping Liu,Jianfeng Pan,Peilian Wei,Jianzhong Zhu,Lei Huang,Jin Cai,Zhinan Xu 한국생물공학회 2012 Biotechnology and Bioprocess Engineering Vol.17 No.4

In order to improve the production of alcohol oxidase (AOX), a recombinant Pichia pastoris (P. pastoris)system was constructed by transformation of the plasmid pPIC9K-AOX into P. pastoris GS115. The effects of different expression conditions on alcohol oxidase activity in the culture supernatant were investigated in the shake flask scale. The results showed that the highest extracellular activity (562 U/L) of alcohol oxidase was obtained after 56h induction with 4% methanol at OD600 1.0 in the medium containing 50 g/L maltose, which is about 4.2 folds higher than previously reported. High-purity functional recombinant AOX (>90%) was purified from the culture with the Ni-NTA affinity column and Sephadex G-100 chromatographical methods, with a total recovery rate of 68.9%. Further studies showed that the purified rAOX had similar enzymatic characteristics as the native enzyme, except that the thermal stability and resistance to H2O2 inhibition of rAOX were significantly greater compared to the previous report. The purified rAOX was well tolerant to various water-miscible organic solvents. This efficient expression and purification process will be promising for large-scale production of rAOX as an important diagnostic enzyme for alcohol detection in many areas.

Effects of high power pulsed microwave on the enhanced color and flavor of aged blueberry wine

Siyuan Liu,Shuangjian Li,Siyuan Li,Ying Wang,Linlin Fan,Jianzhong Zhou 한국식품과학회 2024 Food Science and Biotechnology Vol.33 No.1

A new method of high-power pulsed microwave (HPPM) was applied to accelerate the aging of blueberry wine. The color changes of blueberry wines during aging were investigated through Chemical Wine Age and CIE-LAB measurement. Results showed that the blueberry wines treated by HPPM at low frequencies (50 and 100 Hz) exhibited improved color characteristics with L* value reaching 47.04 at 100 Hz, an increased maturity of wine body, and a shortened chemical wine age from 90 days to 75 days. Moreover, the aroma changes determined by GC-MS showed that HPPM accelerated the formation of esters in blueberry wine, which were increased by 18.44% and 56.97% respectively under the conditions of 50 and 150 Hz. The formation of acid substances was reduced compared with the original wine, with contents of acetic acid, caproic acid, and octanoic acid of 29.46 µg/mL, 15.60 µg/mL, 17.74 µg/mL, respectively, displaying an enhanced wine flavor.

Correlation between Heart-type Fatty Acid-binding Protein Gene Polymorphism and mRNA Expression with Intramuscular Fat in Baicheng-oil Chicken

Yong Wang,Jianzhong He,Wenxuan Yang,Gemenggul Muhantay,Ying Chen,Jinming Xing,Jianzhu Liu 아세아·태평양축산학회 2015 Animal Bioscience Vol.28 No.10

This study aims to determine the polymorphism and mRNA expression pattern of the heart-type fatty acid-binding protein (H-FABP) gene and their association with intramuscular fat (IMF) content in the breast and leg muscles of Baicheng oil chicken (BOC). A total of 720 chickens, including 240 black Baicheng oil chicken (BBOC), 240 silky Baicheng oil chicken (SBOC), and 240 white Baicheng oil chicken (WBOC) were raised. Three genotypes of H-FABP gene second extron following AA, AB, and BB were detected by polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) strategy. The G939A site created AA genotype and G956A site created BB genotype. The content of IMF in AA genotype in breast muscle of BBOC was significantly higher than that of AB (p = 0.0176) and the genotype in leg muscle of WBOC was significantly higher than that of AB (p = 0.0145). The G939A site could be taken as genetic marker for higher IMF content selecting for breast muscle of BBOC and leg muscle of WBOC. The relative mRNA expression of H-FABP was measured by real-time PCR at 30, 60, 90, and 120 d. The IMF content significantly increased with age in both muscles. The mRNA expression level of H-FABP significantly decreased with age in both muscles of the three types of chickens. Moreover, a significant negative correlation between H-FABP abundance and IMF content in the leg muscles of WBOC (p = 0.035) was observed. The mRNA expression of H-FABP negatively correlated with the IMF content in both breast and leg muscles of BOC sat slaughter time.

ZnO Nanorod Array Modified PVDF Membrane with Superhydrophobic Surface for Vacuum Membrane Distillation Application

Wang, Manxiang,Liu, Guicheng,Yu, Hyunjin,Lee, Sang-Hyup,Wang, Lei,Zheng, Jianzhong,Wang, Tao,Yun, Yanbin,Lee, Joong Kee American Chemical Society 2018 ACS APPLIED MATERIALS & INTERFACES Vol.10 No.16

<P>The vacuum membrane distillation (VMD) is a promising technology for lots of applications. To solve the membrane fouling and wetting problems, in this paper, a novel ZnO nanorods 1<I>H</I>,1<I>H</I>,2<I>H</I>,2<I>H</I>-perfluorodecyltriethoxysilane (PDTS) modified poly(vinylidene fluoride) (PVDF) membrane with a micro/nanoscale hierarchical structure and a superhydrophobic surface has been prepared and applied to the VMD process for distilling highly salty water, for the first time. Among these, a pyrolysis-adhesion method is created to obtain the ZnO seeds and fasten them on the PVDF substrate firmly. The novel modified membrane shows a stable superhydrophobic surface with a water contact angle of 152°, easy cleaning property, excellent thermal and mechanical stability, because of the Cassie’s state caused by pocketing much air in the hydrophobized ZnO nanorods, the low surface energy of PDTS coating, and the strong adhesion between ZnO nanorods and PVDF membrane, which has built an ideal structure for VMD application. After 8 h VMD of 200 g L<SUP>-1</SUP> NaCl solution, compared to the virgin PVDF membrane, the novel membrane shows a similar permeate flux but a much higher quality permeated liquid because of its unique antifouling and antiwetting caused by the several microns gap between the feed and the membrane. Due to its easy cleaning property, the novel membrane also exhibits an excellent reusability.</P> [FIG OMISSION]</BR>

Mechanism underlying the effect of conventional drying on the grinding characteristics of Ximeng lignite

Yumeng Yang,Jianzhong Liu,Xiang He,Zhihua Wang,Junhu Zhou,Kefa Cen 한국화학공학회 2017 Korean Journal of Chemical Engineering Vol.34 No.4

Same amounts of moisture were removed from Ximeng lignite (XL) with different particle size ranges pretreated at different drying temperatures. The effect of conventional drying on the grindability of the XLs was investigated. Increasing the drying temperature improved the grindability of all the samples. The results of scanning electron microscopy and mercury intrusion porosimetry revealed that the dominant mechanism enhancing the grindability of XL with high moisture was the pore structure destruction induced by the steam jet flow generated with the removal of moisture. Especially, the development of large fractures had a strong connection with the change in the grindability. According to the pore size distribution, the internal structure of the 2.5-4.0mm coal samples did not develop well under high drying temperature because of the exceedingly short heating time. Therefore, coal particle size, drying temperature, and heating time must be coordinated well to achieve the enhanced drying effect. The grindability of XL had a negative linear correlation with the pore volume fractal dimension, revealing the possibility of fractal dimension for the analysis of lignite grindability.

Adsorption of copper on iminodisuccinic acid modified attapulgite: characterization and mechanism

Qiuzi Zhu,Jianzhong Zhu,Meng Su,Liang Liu,Cunshi Wang,Dongliang Ji,Wangjun Bai,Wentao Shen 대한환경공학회 2023 Environmental Engineering Research Vol.28 No.4

Heavy-metal ions are common pollutants in wastewater and are thus attracting considerable attention. Herein, an eco-friendly biodegradable adsorbent, iminodisuccinic acid (IDS) modified attapulgite (ATP) is prepared by graft-polymerization to reduce Cu(II) in water, referred as IDS-ATP. The equilibrium adsorption capacity of IDS-ATP for Cu(II) is increased by 329.5% and 272% compared with raw ATP and non-degradable chelator ethylenediaminetetraacetic acid-modified ATP (EDTA-ATP), respectively. Moreover, the adsorption capacities for Cu(II) in combined system increased by 186% compared with in single system. The structure and surface properties of IDS-ATP are characterized, demonstrating that the IDS moieties are anchored on the surface of ATP without structural damage. In the aqueous Cu(II) (64 mg /L), the best adsorption pH is 5.0, the best dosage is 800 mg/L, and the adsorption equilibrium time is 4 h. The adsorption of IDS-ATP is chemical adsorption and regenerated adsorbent still exhibits high adsorption capacity. The adsorption mechanism includes the coordination of amino groups with Cu(II), the chelation of -COOH on heavy metals (HMs), and the ion exchange. Taking Cu(II) as an example to study the process of IDS-ATP in water, it is beneficial to apply this degradable material to reduce the other HMs.